Searched for: subject:"Proteinase"
(1 - 20 of 34)

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van der Tuin, S.J. (author), Kühnast, S. (author), Berbée, J. (author), Verschuren, L. (author), Pieterman, E.J. (author), Havekes, L.M. (author), van der Hoorn, J.W. (author), Rensen, P.C. (author), Jukema, J.W. (author), Princen, H.M. (author), Willems van Dijk, K. (author), Wang, Y. (author)
Recently, we showed in APOE*3-Leiden.CETP mice that anacetrapib attenuated atherosclerosis development by reducing (V)LDL-C rather than by raising HDL-C. Here, we investigated the mechanism by which anacetrapib reduces (V)LDL-C and whether this effect was dependent on the inhibition of CETP. APOE*3-Leiden.CETP mice were fed a Western type diet...
article 2015
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Molhoek, E.M. (author), van Dijk, A. (author), Veldhuizen, E.J.A. (author), Haagsman, H.P. (author), Bikker, F.J. (author)
A truncated version of host defense peptide chicken cathelicidin-2, C1-15, possesses potent, broad spectrum antibacterial activity. A variant of this peptide, F2,5,12W, which contains 3 phenylalanine to tryptophan substitutions, possesses improved antibacterial activity and lipopolysaccharide (LPS) neutralizing activity compared to C1-15. In...
article 2011
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Adav, S.S. (author), Li, A.A. (author), Manavalan, A. (author), Punt, P. (author), Sze, S.K. (author), TNO Kwaliteit van Leven (author)
The natural lifestyle of Aspergillus niger made them more effective secretors of hydrolytic proteins and becomes critical when this species were exploited as hosts for the commercial secretion of heterologous proteins. The protein secretion profile of A. niger and its mutant at different pH was explored using iTRAQ-based quantitative proteomics...
article 2010
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Treijtel, N. (author), Eijkeren, J.C.H.v. (author), Nijmeijer, S. (author), van der Greef de - Sandt, I.C.J. (author), Freidig, A.P. (author), TNO Kwaliteit van Leven (author)
The metabolism and active transport of ritonavir and saquinavir were studied using sandwich-cultured rat hepatoyctes and rat liver microsomes. For ritonavir four comparable metabolites were observed in the sandwich-culture and in microsomes. For saquinavir eight metabolites were observed in sandwich-culture and 14 different metabolites in...
article 2009
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Abdul-Hussien, H. (author), Hanemaaijer, R. (author), Verheijen, J.H. (author), van Bockel, J.H. (author), Geelkerken, R.H. (author), Lindeman, J.H.N. (author), TNO Kwaliteit van Leven (author)
Background: Matrix metalloproteinase-9 (MMP-9) is thought to play a central role in abdominal aortic aneurysm (AAA) initiation. Doxycycline, a tetracycline analogue, has direct MMP-9-inhibiting properties in vitro, and it effectively suppresses AAA development in rodents. Observed inhibition of AAA progression, and contradictory findings in...
article 2009
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Plaisier, M. (author), Dennert, I. (author), Rost, E. (author), Koolwijk, P. (author), van Hinsbergh, V.W.M. (author), Helmerhorst, F.M. (author), TNO Kwaliteit van Leven (author)
BACKGROUND : Decidual vascular development is important for implantation. This study analysed decidual vascular adaptation to implantation in correlation with miscarriage in decidual secretory endometrium (DSE), decidua parietalis (DP) and decidua basalis (DB) of miscarriage patients and matched controls. METHODS : Decidua was obtained during...
article 2009
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de Jonge, J.D. (author), Ezendam, J. (author), Knippels, L.M.J. (author), Penninks, A.H. (author), Pieters, R. (author), van Loveren, H. (author), TNO Kwaliteit van Leven (author)
Probiotics are claimed to beneficially affect the immune system and their involvement in allergy prevention is being investigated extensively. However, the efficacy of probiotics in allergy prevention remains controversial. We investigated whether the probiotic Lactobacillus casei Shirota (LcS) could modulate the food allergic response against...
article 2008
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Punt, P.J. (author), Schuren, F.H.J. (author), Lehmbeck, J. (author), Christensen, T. (author), Hjort, C. (author), van den Hondel, C.A.M.J.J. (author), TNO Kwaliteit van Leven (author)
Expression of several Aspergillus niger genes encoding major secreted, but not vacuolar, protease genes including the major acid protease gene pepA, was shown to be affected in the previously isolated A. niger protease mutant, AB1.13 [Mattern, I.E., van Noort, J.M., van den Berg, P., Archer, D.A., Roberts, I.N., Hondel, C.A.M.J.J., 1992....
article 2008
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Plaisier, M. (author), Koolwijk, P. (author), Willems, F. (author), Helmerhorst, F.M. (author), van Hinsbergh, V.W.M. (author), TNO Kwaliteit van Leven (author)
Proteolysis is essential for decidual development during embryonic implantation, but little is known regarding the expression and functions of membrane-type matrix metalloproteinases (MT-MMPs) and urokinase-type plasminogen activator (uPA) and its receptor uPAR in decidua. Therefore, their protein and mRNA levels were analysed in three first...
article 2008
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de Jonge, J.D. (author), Ezendam, J. (author), Knippels, L.M.J. (author), Odink, J. (author), Pourier, M.S. (author), Penninks, A.H. (author), Pieters, R. (author), van Loveren, H. (author), TNO Kwaliteit van Leven (author)
Other factors than the allergen itself may be of importance in the development of food allergy. This report describes the influence of the immunosuppressive compound bis(tributyltin)oxide (TBTO), present in the food chain, on the development of food allergy to peanut or ovalbumin in Brown Norway (BN) rats. To study these effects BN rats were...
article 2007
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Hawinkels, L.J.A.C. (author), Verspaget, H.W. (author), van Duijn, W. (author), van der Zon, J.M. (author), Zuidwijk, K. (author), Kubben, F.J.G.M. (author), Verheijen, J.H. (author), Hommes, D.W. (author), Lamers, C.B.H.W. (author), Sier, C.F.M. (author), TNO Kwaliteit van Leven (author)
Transforming growth factor-β1 (TGF-β1), a tumour suppressing as well as tumour-promoting cytokine, is stored as an extracellular matrix-bound latent complex. We examined TGF-β1 activation and localisation of TGF-β1 activity in gastric cancer. Gastric tumours showed increased stromal and epithelial total TGF-β1 staining by immunohistochemistry....
article 2007
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den Boer, M.A.M. (author), Berbée, J.F.P. (author), Reiss, P. (author), van der Valk, M. (author), Voshol, P.J. (author), Kuipers, F. (author), Havekes, L.M. (author), Rensen, P.C.N. (author), Romijn, J.A. (author), Gaubius Instituut TNO (author)
Objective - The use of the HIV protease inhibitor ritonavir (RTV) is frequently associated with hypertriglyceridemia and lipodystrophy. The aim of our study was to determine the mechanism underlying the observed hypertriglyceridemia. Methods and Results - Feeding female APOE*3-Leiden transgenic mice a Western-type diet supplemented with RTV (35...
article 2006
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van Hinsbergh, V.W.M. (author), Engelse, M.A. (author), Quax, P.H.A. (author), TNO Kwaliteit van Leven (author)
Pericellular proteases play an important role in angiogenesis and vasculogenesis. They comprise (membrane-type) matrix metalloproteinases [(MT-)MMPs], serine proteases, cysteine cathepsins, and membrane-bound aminopeptidases. Specific inhibitors regulate them. Major roles in initiating angiogenesis have been attributed to MT1-matrix...
article 2006
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Pouvreau, L. (author), Gruppen, H. (author), van Koningsveld, G. (author), van den Broek, L.A.M. (author), Voragen, A.G.J. (author), TNO Voeding (author)
The thermal unfolding of potato serine protease inhibitor (PSPI), the most abundant protease inhibitor group in potato tuber, was measured using far UV CD spectroscopy, fluorescence spectroscopy, and DSC. The results indicate that the thermal as well as the guanidinium-induced unfolding of PSPI occurs via a non-two-state mechanism in which at...
article 2005
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te Biesebeke, R. (author), van Biezen, N. (author), de Vos, W.M. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), TNO Kwaliteit van Leven (author)
Solid-state fermentation (SSF) with Aspergillus oryzae results in high levels of secreted protein. However, control mechanisms of gene expression in SSF have been only poorly studied. In this study we show that both glucoamylase (glaB) and protease (alpA, nptB) genes are highly expressed during surface cultivation on wheat-based solid medium,...
article 2005
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te Biesebeke, R. (author), Record, E. (author), van Biezen, N. (author), Heerikhuisen, M. (author), Franken, A. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), TNO Kwaliteit van Leven (author)
To study the relation between the number of hyphal tips and protein secretion during growth on a solid substrate, we have constructed two mutant strains of Aspergillus oryzae with increased hyphal branching. We have analysed hydrolytic enzyme activities during growth on wheat kernels (WK) of A. oryzae strains carrying the disrupted allele of the...
article 2005
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Sluijter, J.P.G. (author), Verloop, R.E. (author), Pulskens, W.P.C. (author), Velema, E. (author), Grimbergen, J.M. (author), Quax, P.H. (author), Goumans, M.J. (author), Pasterkamp, G. (author), de Kleijn, D.P.V. (author), TNO Kwaliteit van Leven (author)
Background: Furin-like proprotein convertases (PCs) are proteolytic activators of proproteins, like membrane type 1-matrix metalloproteinase (MT1-MMP) and transforming growth factor β (TGF-β), that are described in the arterial response to injury. However, the involvement of furin-like PCs in the arterial response to injury has not been studied...
article 2005
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Lindeman, J.H.N. (author), Hanemaaijer, R. (author), Mulder, A. (author), Dijkstra, P.D.S. (author), Szuhai, K. (author), Bromme, D. (author), Verheijen, J.H. (author), Hogendoorn, P.C.W. (author)
Giant cell tumor (GCT) of bone is a neoplasm of bone characterized by a localized osteolytic lesion. The nature of GCT is an enigma and the cell type(s) and protease(s) responsible for the extensive localized clinicoradiological osteolysis remain unresolved. We evaluated protease expression and cellular distribution of the proteolytic machinery...
article 2004
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Rijken, D.C. (author), Barrett-Bergshoeff, M.M. (author), Jie, A.F.H. (author), Criscuoli, M. (author), Sakharov, D.V. (author), Gaubius Instituut TNO (author)
Amediplase (K2tu-PA) is a hybrid plasminogen activator, consisting of the kringle 2 domain of alteplase and the protease domain of urokinase. The objective of this study was to determine the in vitro clot penetration of amediplase in relation to its fibrin binding and to compare the properties with those of alteplase. The clot lysis activity of...
article 2004
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Koppelman, S.J. (author), Lakemond, C.M.M. (author), Vlooswijk, R. (author), Hefle, S.L. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
Several tests for the detection of soy proteins in foods have been described in the literature, and some are commercially available. This article gives an overview of these methods and discusses the advantages and disadvantages of each individual method. Based on the conclusions of this inventory, an experimental approach was designed to improve...
article 2004
Searched for: subject:"Proteinase"
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