Searched for: subject%3A%22Protein%255C%2Bsynthesis%22
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TNO Kwaliteit van Leven (author), Tak, P.P. (author), van Kuijk, A.W.R. (author), de Groot, J. (author), Koeman, R.C. (author), Sakkee, N. (author), Baeten, D.L. (author), Gerlag, D.M. (author)
Background: There is growing interest in soluble biomarkers that could be used on the group level for screening purposes in small proof of principle studies during early drug development. We investigated early changes in serum levels of several candidate biomarkers involved in cartilage and bone metabolism following the initiation of adalimumab...
article 2010
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Rubbens, M.P. (author), Mol, A. (author), van Marion, M.H. (author), Hanemaaijer, R. (author), Bank, R.A. (author), Baaijens, F.P.T. (author), Bouten, C.V.C. (author), TNO Kwaliteit van Leven (author)
Similar to native cardiovascular tissues, the mechanical properties of engineered cardiovascular constructs depend on the composition and quality of the extracellular matrix, which is a net result of matrix remodeling processes within the tissue. To improve tissue remodeling, and hence tissue mechanical properties, various mechanical...
article 2009
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Karnaukhova, E. (author), Ophir, Y. (author), Trinh, L. (author), Dalal, N. (author), Punt, P.J. (author), Golding, B. (author), Shiloach, J. (author), TNO Kwaliteit van Leven (author)
Background: Human α1-proteinase inhibitor (α1-PI), also known as antitrypsin, is the most abundant serine protease inhibitor (serpin) in plasma. Its deficiency is associated with development of progressive, ultimately fatal emphysema. Currently in the United States, α1-PI is available for replacement therapy as an FDA licensed plasma-derived (pd...
article 2007
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Halter, D. (author), Neumann, S. (author), van Dijk, S.M. (author), Wolthoorn, J. (author), de Mazière, A.M. (author), Vieira, O.V. (author), Mattjus, P. (author), Klumperman, J. (author), van Meer, G. (author), Sprong, H. (author), TNO Kwaliteit van Leven (author)
Glycosphingolipids are controlled by the spatial organization of their metabolism and by transport specificity. Using immunoelectron microscopy, we localize to the Golgi stack the glycosyltransferases that produce glucosylceramide (GlcCer), lactosylceramide (LacCer), and GM3. GlcCer is synthesized on the cytosolic side and must translocate...
article 2007
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Bsibsi, M. (author), Bajramovic, J.J. (author), van Duijvenvoorden, E. (author), Persoon, C. (author), Ravid, R. (author), van Noort, J.M. (author), Vogt, M.H.J. (author), TNO Kwaliteit van Leven (author)
Human astrocytes express a limited repertoire of Toll-like receptor (TLR) family members including TLR1-4, which are expressed on the cell surface. Also, TLR3 but not TLR4 activation on astrocytes induces expression of several factors involved in neuroprotection and down-regulation of inflammation rather than in the onset of traditional pro...
article 2007
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Verhoeckx, K.C.M. (author), Korthout, H.A.A.J. (author), van Meeteren-Kreikamp, A.P. (author), Ehlert, K.A. (author), Wang, M. (author), van der Greef, J. (author), Rodenburg, R.J.T. (author), Witkamp, R.F. (author), TNO Kwaliteit van Leven (author)
There is a great interest in the pharmacological properties of cannabinoid like compounds that are not linked to the adverse effects of Δ9-tetrahydrocannabinol (THC), e.g. psychoactive properties. The present paper describes the potential immuno-modulating activity of unheated Cannabis sativa extracts and its main non-psychoactive constituent Δ9...
article 2006
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Schaafsma, G. (author), TNO Kwaliteit van Leven (author)
The increasing prevalence in many countries of people with overweight and obesity is undoubtedly one of the biggest threats to public health. Dietary proteins, because of their positive effects on satiation/ satiety, may help to reduce energy intake and promote a healthy body composition with less body fat. Several mechanisms have been put...
article 2006
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van Erk, M.J. (author), Blom, W.A.M. (author), van Ommen, B. (author), Hendriks, H.F.J. (author), TNO Kwaliteit van Leven (author)
Background: Application of transcriptomics technology in human nutrition intervention studies would allow for genome-wide screening of the effects of specific diets or nutrients and result in biomarker profiles. Objective: The aim was to evaluate the potential of gene expression profiling in blood cells collected in a human intervention study...
article 2006
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Korsheninnikova, E. (author), van der Zon, G.C.M. (author), Voshol, P.J. (author), Janssen, G.M. (author), Havekes, L.M. (author), Grefhorst, A. (author), Kuipers, F. (author), Reijngoud, D.-J. (author), Romijn, J.A. (author), Ouwens, D.M. (author), Maassen, J.A. (author), TNO Kwaliteit van Leven (author)
Aims/hypothesis: Activation of nutrient sensing through mammalian target of rapamycin (mTOR) has been linked to the pathogenesis of insulin resistance. We examined activation of mTOR-signalling in relation to insulin resistance and hepatic steatosis in mice. Materials and methods: Chronic hepatic steatosis and hepatic insulin resistance were...
article 2006
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Mandelin, J. (author), Hukkanen, M. (author), Li, T.F. (author), Korhonen, M. (author), Liljeström, M. (author), Sillat, T. (author), Hanemaaijer, R. (author), Salo, J. (author), Santavirta, S. (author), Konttinen, Y.T. (author), TNO Kwaliteit van Leven (author)
Healthy bone is a rigid yet living tissue that undergoes continuous remodeling. Osteoclasts resorb bone in the remodeling cycle. They secrete H+-ions and proteinases to dissolve bone mineral and degrade organic bone matrix, respectively. One of the main collagenolytic proteinase in osteoclasts is cathepsin K, a member of papain family cysteine...
article 2006
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Weenink, X.O. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Ram, A.F.J. (author), TNO Kwaliteit van Leven (author)
Although filamentous fungi have a unique property of secreting a large amount of homologous extracellular proteins, the use of filamentous fungi as hosts for the production of heterologous proteins is limited because of the low production levels that are generally reached. Here, we report a general screening method for the isolation of mutants...
article 2006
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Schaafsma, G. (author), TNO Kwaliteit van Leven (author)
Loss of muscle mass as a consequence of changes in protein metabolism during periods of catabolic stress is a serious complication in a variety of conditions. These conditions are weight loss programs, sarcopenia in the elderly and several clinical states. It appears from many studies that improved protein nutrition by ingestion of high-quality...
article 2006
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TNO Kwaliteit van Leven (author), Wildwater, M. (author), Campilho, A. (author), Perez-Perez, J.M. (author), Heidstra, R. (author), Blilou, I. (author), Korthout, H. (author), Chatterjee, J. (author), Mariconti, L. (author), Gruissem, W. (author), Scheres, B. (author)
The maintenance of stem cells in defined locations is crucial for all multicellular organisms. Although intrinsic factors and signals for stem cell fate have been identified in several species, it has remained unclear how these connect to the ability to reenter the cell cycle that is one of the defining properties of stem cells. We show that...
article 2005
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Ram, A.F.J. (author), Arentshorst, M. (author), Damveld, R.A. (author), van Kuyk, P.A. (author), Klis, F.M. (author), van den Hondel, C.A.M.J.J. (author), TNO Voeding (author)
Perturbation of cell wall synthesis in Saccharomyces cerevisiae, either by mutations in cell wall synthesis-related genes or by adding compounds that interfere with normal cell wall assembly, triggers a compensatory response to ensure cell wall integrity. This response includes an increase in chitin levels in the cell wall. Here it is shown that...
article 2004
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Sigoillot, C. (author), Record, E. (author), Belle, V. (author), Robert, J.L. (author), Levasseur, A. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Fournel, A. (author), Sigoillot, J.C. (author), Asther, M. (author), TNO Voeding (author)
Three laccases, a natural form and two recombinant forms obtained from two different expression hosts, were characterized and compared for paper pulp bleaching. Laccase from Pycnoporus cinnabarinus, a well known lignolytic fungus, was selected as a reference for this study. The corresponding recombinant laccases were produced in Aspergillus...
article 2004
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Heijne, W.H.M. (author), Slitt, A.L. (author), van Bladeren, P.J. (author), Groten, J.P. (author), Klaassen, C.D. (author), Stierum, R.H. (author), van Ommen, B. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
Rats were exposed to three levels of bromobenzene, sampled at 6, 24, and 48 h, and liver gene expression profiles were determined to identify dose and time-related changes. Expression of many genes changed transiently, and dependent on the dose. Few changes were identified after 6 h, but many genes were differentially expressed after 24 h, while...
article 2004
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Joosten, V. (author), Lokman, C. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), TNO Voeding (author)
In this review we will focus on the current status and views concerning the production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will focus on single-chain antibody fragment production (scFv and VHH) by these lower eukaryotes and the possible applications of these proteins. Also the coupling of...
article 2003
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Heijne, W.H.M. (author), Stierum, R.H. (author), Slijper, M. (author), van Bladeren, P.J. (author), van Ommen, B. (author)
Toxicogenomics is a novel approach integrating the expression analysis of thousands of genes (transcriptomics) or proteins (proteomics) with classical methods in toxicology. Effects at the molecular level are related to pathophysiological changes of the organisms, enabling detailed comparison of mechanisms and early detection and prediction of...
article 2003
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Centraal Instituut voor Voedingsonderzoek TNO (author), Gennari, A. (author), Bol, M. (author), Seinen, W. (author), Penninks, A. (author), Pieters, R. (author)
The organotin compounds di-n-butyltin dichloride (DBTC) and tri-n-butyltin chloride (TBTC) induce thymus atrophy in rats. At low doses they inhibit immature thymocyte proliferation, whereas at higher doses in particular TBTC induces apoptotic cell death. In vitro, a similar concentration-effect relationship was observed, i.e. low concentrations...
article 2002
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Noort, D. (author), Hulst, A.G. (author), Fidder, A. (author), van Gurp, R.A. (author), de Jong, L.P.A. (author), Benschop, H.P. (author), Prins Maurits Laboratorium TNO (author)
The development of procedures for retrospective detection and quantitation of exposure to phosgene, based on adducts to hemoglobin and albumin, is described. Upon incubation of human blood with [14C]phosgene (0-750 μM), a significant part of radioactivity (0-13%) became associated with globin and albumin. Upon Pronase digestion of globin, one of...
article 2000
Searched for: subject%3A%22Protein%255C%2Bsynthesis%22
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