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de Jong, G.A.H. (author), Jayasena, S. (author), Johnson, P. (author), Marsh, J. (author), Apostolovic, D. (author), van Hage, M. (author), Nordlee, J. (author), Baumert, J. (author), Taylor, S.L. (author), Roucairol, C. (author), de Jongh, H. (author), Koppelman, S.J. (author)The 2S albumin Ara h 6 is one of the most important peanut allergens. A post-translationally cleaved Ara h 6 (pAra h 6) was purified from Virginia type peanuts, and the cleavage site was mapped using high-resolution mass spectrometry. Compared to intact Ara h 6, pAra h 6 lacks a 5-amino acid stretch, resembling amino acids 43-47 (UniProt...article 2018
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Apostolovic, D. (author), Luykx, D. (author), Warmenhoven, H. (author), Verbart, D. (author), Stanic-Vucinic, D. (author), de Jong, G.A.H. (author), Velickovic, T.C. (author), Koppelman, S.J. (author)Conglutins, the major peanut allergens, Ara h 2 and Ara h 6, are highly structured proteins stabilized by multiple disulfide bridges and are stable towards heat-denaturation and digestion. We sought a way to reduce their potent allergenicity in view of the development of immunotherapy for peanut allergy. Isoforms of conglutin were purified,...article 2013
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Lindeman, J.H.N. (author), Ashcroft, B.A. (author), Beenakker, J.-W.M. (author), van Es, M. (author), Koekkoek, N.B.R. (author), Prins, F.A. (author), Tielemans, J.F. (author), Abdul-Hussien, H. (author), Bank, R.A. (author), Oosterkamp, T.H. (author), TNO Kwaliteit van Leven (author)An aneurysm of the aorta is a common pathology characterized by segmentalweakeningof the artery.Althoughit isgenerally accepted that the vessel-wall weakening is caused by an impaired collagen metabolism, a clear association has been demonstrated only for rare syndromes such as the vascular type Ehlers-Danlos syndrome. Here we show that vessel...article 2010
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van Noort, J.M. (author), TNO Kwaliteit van Leven (author)Stress proteins or heat shock proteins (HSPs) are ubiquitous cellular components that have long been known to act as molecular chaperones. By assisting proper folding and transport of proteins, and by assisting in the degradation of aberrant proteins, they play key roles in cellular metabolism. The frequent accumulation of insoluble protein...article 2008
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Kudryashova, E.V. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author), KvL (author)The possibility of modulating the mesoscopic properties of food colloidal systems by the dielectric constant is studied by determining the impact of small amounts of ethanol (10%) on the adsorption of egg white ovalbumin onto the air-water interface in the absence and presence of pectin. The adsorption kinetics was monitored using tensiometry....article 2008
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van Boxtel, E.L. (author), van den Broek, L.A.M. (author), Koppelman, S.J. (author), Gruppen, H. (author), TNO Kwaliteit van Leven (author)Legumin proteins Ara h 3 from peanuts and glycinin from soybeans are increasingly described as important allergens. The stability of an allergen's IgE binding capacity towards heating and digestion is considered an important characteristic for food allergens. We investigated the effects of heating and digestion on the IgE binding of Ara h 3 and...article 2008
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Glycolipid-dependent sorting of melanosomal from lysosomal membrane proteins by lumenal determinantsGroux-Degroote, S. (author), van Dijk, S.M. (author), Wolthoorn, J. (author), Neumann, S. (author), Theos, A.C. (author), de Mazière, A.M. (author), Klumperman, J. (author), van Meer, G. (author), Sprong, H. (author), TNO Kwaliteit van Leven (author)Melanosomes are lysosome-related organelles that coexist with lysosomes in mammalian pigment cells. Melanosomal and lysosomal membrane proteins share similar sorting signals in their cytoplasmic tail, raising the question how they are segregated. We show that in control melanocytes, the melanosomal enzymes tyrosinase-related protein 1 (Tyrp1)...article 2008
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Weijers, M. (author), Broersen, K. (author), Barneveld, P.A. (author), Cohen Stuart, M.A. (author), Hamer, R.J. (author), de Jongh, H.H.J. (author), Visschers, R.W. (author), TNO Kwaliteit van Leven (author)The effect of ovalbumin net charge on aggregate morphology and visual properties was investigated using chromatography, electrophoresis, electron microscopy, and turbidity measurements. A range of differently charged ovalbumin variants (net charge ranging from -1 to -26 at pH 7) was produced using chemical engineering. With increasing net charge...article 2008
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Emeis, J.J. (author), Jirouskova, M. (author), Muchitsch, E.-M. (author), Shet, A.S. (author), Smyth, S.S. (author), Johnson, G.J. (author), TNO Preventie en Gezondheid (author)Murine blood coagulation factors and function are quite similar to those of humans. Because of this similarity and the adaptability of mice to genetic manipulation, murine coagulation factors and inhibitors have been extensively studied. These studies have provided significant insights into human hemostasis. They have also provided useful...article 2007
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Wierenga, P.A. (author), Egmond, M.R. (author), Voragen, A.G.J. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)Unfolding of proteins has often been mentioned as an important factor during the adsorption process at air-water interfaces and in the increase of surface pressure at later stages of the adsorption process. This work focuses on the question whether the folding state of the adsorbed protein depends on the rate of adsorption to the interface,...article 2006
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Kaijzel, E.L. (author), Koolwijk, P. (author), van Erck, M.G.M. (author), van Hinsbergh, V.W.M. (author), de Maat, M.P.M. (author), TNO Kwaliteit van Leven (author)Background: During wound repair, fibrin acts both as a barrier to prevent blood loss and as a temporary matrix for the invasion and ingrowth of endothelial and tissue cells. A well-controlled angiogenesis process in the fibrinous exudate matrix is crucial for optimal wound healing. The composition and structure of the fibrin matrix are important...article 2006
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van Boxtel, E.L. (author), van Beers, M.M.C. (author), Koppelman, S.J. (author), van den Broek, L.A.M. (author), Gruppen, H. (author), TNO Kwaliteit van Leven (author)Ara h 1, a major peanut allergen, is known as a stable trimeric protein. Nevertheless, upon purification of native Ara h 1 from peanuts using only size exclusion chromatography, the allergen appeared to exist in an oligomeric structure, rather than as a trimeric structure. The oligomeric structure was independent of the salt concentration...article 2006
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Kudryashova, E.V. (author), Visser, A.J.W.G. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)In this study the relation between the ability of protein self-association and the surface properties at air-water interfaces is investigated using a combination of spectroscopic techniques. Three forms of chicken egg ovalbumin were obtained with different self-associating behavior: native ovalbumin, heat-treated ovalbumin-being a cluster of 12...article 2005
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Zuurmond, A.M. (author), Slot van der-Verhoeven, A.J. (author), van Dura, E.A. (author), de Groot, J. (author), Bank, R.A. (author), TNO Kwaliteit van Leven (author)Collagen deposits in fibrotic lesions often display elevated levels of hydroxyallysine (pyridinoline) cross-links. The relation between the occurrence of pyridinoline cross-links and the irreversibility of fibrosis suggests that these cross-links contribute to the aberrant accumulation of collagen. Based on its inhibitory effect on lysyl...article 2005
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Wierenga, P.A. (author), Meinders, M.B.J. (author), Egmond, M.R. (author), Voragen, A.G.J. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)In this study a set of chemically engineered variants of ovalbumin was produced to study the effects of electrostatic charge on the adsorption kinetics and resulting surface pressure at the air-water interface. The modification itself was based on the coupling of succinic anhydride to lysine residues on the protein surface. After purification of...article 2005
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Koppelman, S.J. (author), Nieuwenhuizen, W.F. (author), Gaspari, M. (author), Knippels, L.M.J. (author), Penninks, A.H. (author), Knol, E.F. (author), Hefle, S.L. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)The high resistance of Brazil nut 2S albumin, previously identified as an allergen, against proteolysis by pepsin was examined in this work. Although the denaturation temperature of this protein exceeds the 110 °C at neutral pH, at low pH a fully reversible thermal denaturation was observed at ∼82 °C. The poor digestibility of the protein by...article 2005
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van Teeffelen, A.M.M. (author), Broersen, K. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)Chemical glycosylation of proteins occurs in vivo spontaneously, especially under stress conditions, and has been linked in a number of cases to diseases related to protein denaturation and aggregation. It is the aim of this work to study the origin of the change in thermodynamic properties due to glucosylation of the folded β-lactoglobulin A....article 2005
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Broersen, K. (author), Voragen, A.G.J. (author), Hamer, R.J. (author), de Jongh, H.H.J. (author), TNO Voeding (author)In this article we show how various degrees of glycosylation can be used to control the thermal stability of proteins. The primary amines of β-lactoglobulin were glycosylated with glucose or fructose within a range of non-denaturing reaction parameters. The modified fractions were characterized and analyzed for structural stability and...article 2004
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Knippels, L.M.J. (author), van Wijk, F. (author), Penninks, A.H. (author), TNO Voeding (author)Purpose of review This review summarizes selected articles on animal models of food allergy published in 2003. The research areas that are covered include mechanistic studies, the search for new therapies, as well as screening models for hazard identification of potential allergens. Recent findings Novel treatment options of both prevention and...article 2004
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Kralj, S. (author), van Geel-Schutten, G.H. (author), Dondorff, M.M.G. (author), Kirsanovs, S. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)Members of the genera Streptococcus and Leuconostoc synthesize various α-glucans (dextran, alternan and mutan). In Lactobacillus, until now, the only glucosyltransferase (GTF) enzyme that has been characterized is gtfA of Lactobacillus reuteri 121, the first GTF enzyme synthesizing a glucan (reuteran) that contains mainly α-(1 → 4) linkages...article 2004