Searched for: subject%3A%22Protein%255C%2Bsecretion%22
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van Avesaat, M. (author), Troost, F.J. (author), Ripken, D. (author), Hendriks, H.F. (author), Aam, M. (author)
BACKGROUND: Activation of the ileal brake, by infusing lipid directly into the distal part of the small intestine, alters gastrointestinal (GI) motility and inhibits food intake. The ileal brake effect on eating behavior of the other macronutrients is currently unknown. OBJECTIVE: The objective of this study was to investigate the effects of...
article 2015
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van Avesaat, M. (author), Troost, F.J. (author), Ripken, D. (author), Peters, J. (author), Hendriks, H.F.J. (author), Masclee, A.A.M. (author)
Background: Taste receptors are expressed not only in taste buds but also in the gastrointestinal tract. It has been hypothesized that these receptors may play a role in satiety and food intake. Objective: This study investigated the effect of intraduodenal tastant infusions (bitter, sweet, and umami) on food intake, hunger and fullness,...
article 2015
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van Noort, J.M. (author), Bsibsi, M. (author), Gerritsen, W.H. (author), van der Valk, P. (author), Bajramovic, J.J. (author), Steinman, L. (author), Amor, S. (author), TNO Kwaliteit van Leven (author)
We present the first comparative analysis of serum immunoglobulinG reactivity profiles against the full spectrum of human myelin-associated proteins in multiple sclerosis patients and healthy control subjects. In both groups, serum antibodies display a consistent and prominent reaction to αB-crystallin (CRYAB) versus other myelin proteins. As an...
article 2010
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Hamer, H.M. (author), Jonkers, D.M.A.E. (author), Renes, I.B. (author), Vanhoutvin, S.A.L.W. (author), Kodde, A. (author), Troost, F.J. (author), Venema, K. (author), Brummer, R.-J.M. (author), TNO Kwaliteit van Leven (author)
INTRODUCTION: The colonic mucus layer plays an important role in the protection of the intestinal epithelium and mainly consists of mucin glycoproteins (primarily MUC2 in the colon) trefoil factor 3 (TFF3) and secretory IgA. Butyrate is a major end product of fermentation of dietary fibres and is associated with beneficial effects on colonic...
article 2010
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Rubbens, M.P. (author), Mol, A. (author), van Marion, M.H. (author), Hanemaaijer, R. (author), Bank, R.A. (author), Baaijens, F.P.T. (author), Bouten, C.V.C. (author), TNO Kwaliteit van Leven (author)
Similar to native cardiovascular tissues, the mechanical properties of engineered cardiovascular constructs depend on the composition and quality of the extracellular matrix, which is a net result of matrix remodeling processes within the tissue. To improve tissue remodeling, and hence tissue mechanical properties, various mechanical...
article 2009
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Li, Y. (author), de Vries, R. (author), Slaghek, T. (author), Timmermans, J. (author), Cohen Stuart, M.A. (author), Norde, W. (author), TNO Kwaliteit van Leven (author)
A novel biocompatible and biodegradable microgel system has been developed for controlled uptake and release of especially proteins. It contains TEMPO-oxidized potato starch polymers, which are chemically cross-linked by sodium trimetaphosphate (STMP). Physical chemical properties have been determined for microgels of different weight ratios of...
article 2009
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Neyraud, E. (author), Heinzerling, C.I. (author), Bult, J.H.F. (author), Mesmin, C. (author), Dransfield, E. (author), TNO Kwaliteit van Leven (author)
Saliva from parotid glands plays a role in taste perception. Parotid saliva is also stimulated by tastants. The aim of this work is to investigate the effects of different tastants on the parotid salivary response in six subjects. Five tastants were given in different concentrations in solution and held in the mouth for 10 s. The flow rate,...
article 2009
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Henneman, P. (author), Schaap, F.G. (author), Havekes, L.M. (author), Rensen, P.C.N. (author), Frants, R.R. (author), van Tol, A. (author), Hattori, H. (author), Smelt, A.H.M. (author), van Dijk, K.W. (author), TNO Kwaliteit van Leven (author)
Objective: The recently discovered apoAV is hypothesized to affect triglyceride metabolism by stimulating the lipolysis of triglycerides in VLDL and chylomicrons. We set out to determine the association between increased serum TG levels, plasma apoAV levels, and polymorphism of the APOA5 gene, with specific emphasis on the APOA5 S19W variation....
article 2007
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Mandelin, J. (author), Hukkanen, M. (author), Li, T.F. (author), Korhonen, M. (author), Liljeström, M. (author), Sillat, T. (author), Hanemaaijer, R. (author), Salo, J. (author), Santavirta, S. (author), Konttinen, Y.T. (author), TNO Kwaliteit van Leven (author)
Healthy bone is a rigid yet living tissue that undergoes continuous remodeling. Osteoclasts resorb bone in the remodeling cycle. They secrete H+-ions and proteinases to dissolve bone mineral and degrade organic bone matrix, respectively. One of the main collagenolytic proteinase in osteoclasts is cathepsin K, a member of papain family cysteine...
article 2006
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te Biesebeke, R. (author), Boussier, A. (author), van Biezen, N. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), TNO Kwaliteit van Leven (author)
Filamentous growth of Aspergillus oryzae on solid cereal substrates involves secretion of substrate converting enzymes and a solid substrate specific polarised hyphal growth phenotype. To identify proteins produced under these specific conditions, the extracts of A. oryzae grown on wheat-based media were analysed using N-terminal sequence...
article 2006
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Verhoeckx, K.C.M. (author), Gaspari, M. (author), Bijlsma, S. (author), van der Greef, J. (author), Witkamp, R.F. (author), Doornbos, R.P. (author), Rodenburg, R.J.T. (author), TNO Kwaliteit van Leven (author)
Two-dimensional difference gel electrophoresis (DIGE) in combination with univariate (Student's t-test) and multivariate data analysis, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used to study the anti-inflammatory effects of the β2-adrenergic receptor (β2-AR) agonist zilpaterol. U937...
article 2005
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te Biesebeke, R. (author), Record, E. (author), van Biezen, N. (author), Heerikhuisen, M. (author), Franken, A. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), TNO Kwaliteit van Leven (author)
To study the relation between the number of hyphal tips and protein secretion during growth on a solid substrate, we have constructed two mutant strains of Aspergillus oryzae with increased hyphal branching. We have analysed hydrolytic enzyme activities during growth on wheat kernels (WK) of A. oryzae strains carrying the disrupted allele of the...
article 2005
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TNO Kwaliteit van Leven (author), Grefhorst, A. (author), Hoekstra, J. (author), Derks, T.G.J. (author), Ouwens, D.M. (author), Baller, J.F.W. (author), Havinga, R. (author), Havekes, L.M. (author), Romijn, J.A. (author), Kuipers, F. (author)
Accumulation of triglycerides (TG) in the liver is generally associated with hepatic insulin resistance. We questioned whether acute hepatic steatosis induced by pharmacological blockade of β-oxidation affects hepatic insulin sensitivity, i.e., insulin-mediated suppression of VLDL production and insulin-induced activation of phosphatidylinositol...
article 2005
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Al-Sheikh, H. (author), Watson, A.J. (author), Lacey, G.A. (author), Punt, P.J. (author), MacKenzie, D.A. (author), Jeenes, D.J. (author), Pakula, T. (author), Penttilä, M. (author), Alcocer, M.J.C. (author), Archer, D.B. (author), TNO Voeding (author)
We describe a new endoplasmic reticulum (ER)-associated stress response in the filamentous fungus Aspergillus niger. The inhibition of protein folding within the ER leads to cellular responses known collectively as the unfolded protein response (UPR) and we show that the selective transcriptional downregulation of the gene encoding glucoamylase,...
article 2004
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Levasseur, A. (author), Pagès, S. (author), Fierobe, H.P. (author), Navarro, D. (author), Punt, P. (author), Belaïch, J.P. (author), Asther, M. (author), Record, E. (author)
A chimeric enzyme associating feruloyl esterase A (FAEA) from Aspergilhis niger and dockerin from Clostridium thermocellum was produced in A. niger. A completely truncated form was produced when the dockerin domain was located downstream of the FAEA (FAEA-Doc), whereas no chimeric protein was produced when the bacterial dockerin domain was...
article 2004
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Punt, P.J. (author), Drint-Kuijvenhoven, A. (author), Lokman, B.C. (author), Spencer, J.A. (author), Jeenes, D. (author), Archer, D.A. (author), van den Hondel, C.A.M.J.J. (author)
We have characterized growth and protein processing characteristics of Aspergillus niger strains carrying a disrupted allele of the previously cloned and characterized kexB gene [Appl. Environ. Microbiol. 66 (2000) 363] encoding a furin-type endoprotease. Deletion of the single-copy gene confirms it to be non-essential but disruptant strains...
article 2003
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Schulter, V. (author), Koolwijk, P. (author), Peters, E. (author), Frank, S. (author), Hrzenjak, A. (author), Graier, W.F. (author), van Hinsbergh, V.W.M. (author), Kostner, G.M. (author), Gaubius Instituut TNO (author)
Angiostatin, which consists of the kringle I-IV domains of plasminogen and which is secreted into urine, is an efficient inhibitor of angiogenesis and tumor growth. Because N-terminal apolipoprotein(a) [apo(a)] fragments, which also contain several types of kringle IV domains, are found in urine as well, we evaluated the potential angiostatic...
article 2001
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Sierksma, A. (author), van der Gaag, M.S. (author), Kluft, C. (author), Hendriks, H.F.J. (author), TNO Voeding (author)
In a diet-controlled, crossover trial with 10 middle-aged men and 9 postmenopausal women, baseline concentrations of fibrinogen influenced the magnitude of decrease of fibrinogen after moderate alcohol consumption. The mechanism of reduction is specific for fibrinogen and unrelated to a reduction in C-reactive protein.
conference paper 2001
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Krasevec, N. (author), van de Hondel, C.A.M.J.J. (author), Komel, R. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A gene-fusion expression strategy was applied for heterologous expression of human lymphotoxin alpha (LTα) in the Aspergillus niger AB1.13 protease-deficient strain. The LTα gene was fused with the A. niger glucoamylase GII-form as a carrier-gene, behind its transcription control and secretion signals. Special attention was paid to the influence...
article 2000
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Krasevec, N. (author), van den Hondel, C.A.M.J.J. (author), Komel, R. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A gene-fusion expression strategy was applied for the heterologous expression of hTNF-α in A. niger AB1.13. The TNF-α gene was fused with the A. niger glucoamylase GII form as a carrier-gene, behind its transcription control and secretion signal. The protein was expressed in the cells in the form of a glucoamylase-fusion protein, but was not...
article 2000
Searched for: subject%3A%22Protein%255C%2Bsecretion%22
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