Searched for: subject:"Protein%5C+domain"
(1 - 10 of 10)
document
Kuhlmann, K.F.D. (author), van Geer, M.A. (author), Bakker, C.T. (author), Dekker, J.E.M. (author), Havenga, M.J.E. (author), Oude Elferink, R.P.J. (author), Gouma, D.J. (author), Bosma, P.J. (author), Wesseling, J.G. (author), TNO Kwaliteit van Leven (author)
Survival of patients with pancreatic cancer is poor. Adenoviral (Ad) gene therapy employing the commonly used serotype 5 reveals limited transduction efficiency due to the low amount of coxsackie-adenovirus receptor on pancreatic cancer cells. To identify fiber-chimeric adenoviruses with improved gene transfer, a library of Ad vectors based on...
article 2009
document
Goosen, C. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)
The purified exo-inulinase enzyme of Aspergillus niger N402 (AngInuE; heterologously expressed in Escherichia coli) displayed a sucrose:inulin (S/I) hydrolysis ratio of 2.3, characteristic for a typical exo-inulinase. The enzyme also had significant transfructosylating activity with increasing sucrose concentrations, producing various...
article 2008
document
Bansal, S. (author), Gaspari, M. (author), Raj, H.G. (author), Kumar, A. (author), Cuda, G. (author), Verheij, E. (author), Tyagi, Y.K. (author), Ponnan, P. (author), Rastogi, R.C. (author), Parmar, V.S. (author), TNO Kwaliteit van Leven (author)
Our earlier investigations identified acetoxy drug: protein transacetylase (TAase), a unique enzyme in the endoplasmic reticulum (ER) catalyzing the transfer of acetyl groups from polyphenolic acetates (PA) to certain functional proteins. Recently we have established the identity of TAase with ER protein calreticulin (CR) and subsequently...
article 2008
document
Levasseur, A. (author), Pagès, S. (author), Fierobe, H.-P. (author), Navarro, D. (author), Punt, P. (author), Belaïch, J.-P. (author), Asther, M. (author), Record, E. (author), TNO Voeding (author)
A chimeric enzyme associating feruloyl esterase A (FAEA) from Aspergilhis niger and dockerin from Clostridium thermocellum was produced in A. niger. A completely truncated form was produced when the dockerin domain was located downstream of the FAEA (FAEA-Doc), whereas no chimeric protein was produced when the bacterial dockerin domain was...
article 2004
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Smit, E. (author), Pouwels, P.H. (author)
The N-terminal repeat (SAC1) of the S-protein of Lactobacillus acidophilus bound efficiently and specifically to cell wall fragments (CWFs) when fused to green fluorescent protein, whereas the C-terminal repeat (SAC2) did not. Treatment of CWFs with hydrofluoric acid, but not phenol, prevented binding. Apparently, SAC1 is necessary and...
article 2002
document
van der Maarel, M.J.E.C. (author), van der Veen, B. (author), Uitdehaag, J.C.M. (author), Leemhuis, H. (author), Dijkhuizen, L. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Starch is a major storage product of many economically important crops such as wheat, rice, maize, tapioca, and potato. A large-scale starch processing industry has emerged in the last century. In the past decades, we have seen a shift from the acid hydrolysis of starch to the use of starch-converting enzymes in the production of maltodextrin,...
article 2002
document
Verzijl, N. (author), de Groot, J. (author), Bank, R.A. (author), Bayliss, M.T. (author), Bijlsma, J.W.J. (author), Lafeber, F.P.J.G. (author), Maroudas, A. (author), TeKoppele, J.M. (author), Gaubius Instituut (author)
During aging, non-enzymatic glycation results in the formation and accumulation of the advanced glycation endproduct pentosidine in long-lived proteins, such as articular cartilage collagen. In the present study, we investigated whether pentosidine accumulation also occurs in cartilage aggrecan. Furthermore, pentosidine levels in aggrecan...
article 2001
document
Kuiper, J. (author), van 't Hof, A. (author), Otter, M. (author), Biessen, E.A.L. (author), Rijken, D.C. (author), van Berkel, T.J.C. (author), Division of Biopharmaceutics, Leiden/Amsterdam Center for Drug Research, Sylvius Laboratory, P. O. Box 9503, 2300 RA Leiden, Netherlands Gaubius Laboratory, TNO Prevention and Health, P.O. Box 430, 2300 AK Leiden, Netherlands (author)
The fibrin-specific thrombolyticum tissue-type plasminogen activator (t-PA) has proven to be a potent drug in several clinical trials, but its clinical application is complicated by the rapid clearance of t-PA from the circulation. The rapid plasma clearance of t-PA results from the uptake of t-PA in the liver. t-PA consists of several domains...
article 1996
document
de Munk, G.A.W. (author), Molinari, A. (author), Rijken, D.C. (author), Gaubius Laboratory Netherlands Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
Chemicals/CAS: amino acid, 65072-01-7; prourokinase, 82657-92-9; thrombin, 9002-04-4; thrombomodulin, 112049-68-0; Enzyme Precursors; Receptors, Cell Surface; Receptors, Thrombin; saruplase, 99149-95-8; Thrombin, EC 3.4.21.5; Urinary Plasminogen Activator, EC 3.4.21.73
article 1993
document
Emeis, J.J. (author), Verheijen, J.H. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)
Chemicals/CAS: Recombinant Proteins; Tissue Plasminogen Activator, EC 3.4.21.68
article 1992
Searched for: subject:"Protein%5C+domain"
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