Searched for: subject:"Protein%5C+Binding"
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Noort, D. (author), Hulst, A.G. (author), Fidder, A. (author), van Gurp, R.A. (author), de Jong, L.P.A. (author), Benschop, H.P. (author), Prins Maurits Laboratorium TNO (author)
The development of procedures for retrospective detection and quantitation of exposure to phosgene, based on adducts to hemoglobin and albumin, is described. Upon incubation of human blood with [14C]phosgene (0-750 μM), a significant part of radioactivity (0-13%) became associated with globin and albumin. Upon Pronase digestion of globin, one of...
article 2000
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Noort, D. (author), Hulst, A.G. (author), Jansen, R. (author), Prins Maurits Laboratorium TNO (author)
Covalent binding of various clinically important nitrogen mustards to the cysteine-34 residue of human serum albumin, in vitro and in vivo, is demonstrated. A rapid method for detection of these adducts is presented, based on liquid chromatography-tandem mass spectrometry analysis of the adducted tripeptide Cys*-Pro-Phe after digestion of the...
article 2002
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Broersen, K. (author), Voragen, A.G.J. (author), Hamer, R.J. (author), de Jongh, H.H.J. (author), TNO Voeding (author)
In this article we show how various degrees of glycosylation can be used to control the thermal stability of proteins. The primary amines of β-lactoglobulin were glycosylated with glucose or fructose within a range of non-denaturing reaction parameters. The modified fractions were characterized and analyzed for structural stability and...
article 2004
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de Man, F.H.A.F. (author), de Beer, F. (author), van der Laarse, A. (author), Smelt, A.H.M. (author), Havekes, L.M. (author), Gaubius Instituut TNO (author)
An in vitro assay to study lipolysis of very low density lipoproteins (VLDL) by heparan sulfate proteoglycan (HSPG-bound lipoprotein lipase (LPL) was developed. Optimal conditions for VLDL lipolysis by HSPG-bound LPL were obtained by incubating plastic wells with 0.5 μg HSPG and 1.5 μg LPL, subsequently. Control experiments with heparinase...
article 1997
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Damsten, M.C. (author), Commandeur, J.N.M. (author), Fidder, A. (author), Hulst, A.G. (author), Touw, D. (author), Noort, D. (author), Vermeulen, N.P.E. (author), TNO Defensie en Veiligheid (author)
Covalent binding of reactive electrophilic intermediates to proteins is considered to play an important role in the processes leading to adverse drug reactions and idiosyncratic drug reactions. Consequently, both for the discovery and the development of new drugs, there is a great interest in sensitive methodologies that enable the detection of...
article 2007
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Smit, E. (author), Oling, F. (author), Demel, R. (author), Martinez, B. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Lactobacillus acidophilus, like many other bacteria, harbors a surface layer consisting of a protein (SA-protein) of 43 kDa. SA-protein could be readily extracted and crystallized in vitro into large crystalline patches on lipid monolayers with a net negative charge but not on lipids with a net neutral charge. Reconstruction of the S-layer from...
article 2001
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Kosters, H.A. (author), Broersen, K. (author), de Groot, J. (author), Simons, J.W.F.A. (author), Wierenga, P. (author), de Jongh, H.H.J. (author), TNO Voeding (author)
Processing of ovalbumin may result in proteins that differ more than 23°C in denaturation temperature while the structural fold is not significantly affected. This is achieved by 1) conversion of positive residues into negative ones (succinylation); 2) elimination of negative charges (methylation); 3) reducing the proteins hydrophobic exposure ...
article 2003
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van der Schans, G.P. (author), Noort, D. (author), Mars-Groenendijk, R.H. (author), Fidder, A. (author), Chau, L.F. (author), de Jong, L.P.A. (author), Benschop, H.P. (author), Prins Maurits Laboratorium TNO (author)
As part of a program to develop methods for diagnosis of exposure to chemical warfare agents, we developed immunochemical methods for detection of adducts of sulfur mustard to keratin in human skin. Three partial sequences of keratins containing glutamine or asparagine adducted with a 2-hydroxyethylthioethyl group at the ω-amide function were...
article 2002
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Noort, D. (author), Hulst, A.G. (author), de Jong, L.P.A. (author), Benschop, H.P. (author)
To develop a mass spectrometric assay for the detection of sulfur mustard adducts with human serum albumin, the following steps were performed: quantitation of the binding of the agent to the protein by using [14C] sulfur mustard and analysis of acidic and tryptic digests of albumin from blood after exposure to sulfur mustard for identification...
article 1999
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Arts, C.J.M. (author), Govers, C.A.R.L. (author), van den Berg, H. (author), Wolters, M.G.E. (author), van Leeuwen, P. (author), Thijsen, J.H. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)
Within the framework of experiments related to the association between dietary fiber and breast cancer an in vitro test system was used to study the binding of estrogens to various fibers (e.g. cholestyramin, lignin and cellulose) and fiber sources (e.g. wheat bran, cereals, seeds and legumes). Furthermore, the in vivo apparent digestibility of...
article 1991
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Otter, M. (author), van Berkel, T.J.C. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
In this study, binding and degradation of tissue-type plasminogen activator (t-PA) by the human hepatoma cell line Hep G2 was investigated. Binding at 4°C was time-dependent and reached a maximum after ca. 2 hours. Scatchard analysis of saturation experiments showed about 170,000 high affinity binding sites for t-PA per cell with an apparent Kd...
article 1989
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van Noort, J.M. (author), van den Berg, P. (author), Mattern, I.E. (author), Medisch Biologisch Laboratorium TNO (author)
A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel...
article 1991
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Nieuwenhuizen, W. (author), Voskuilen, M. (author), Hermans, J. (author), Gaubius instituut TNO (author)
The present study was undertaken as a step to delineate further the localization of the calcium-binding sites in fibrinogen and to assess the anticlotting properties of fibrinogen degradation products. To this purpose, fragments Y were prepared by plasmin digestion of human fibrinogen in the presence of added Ca2+, and purified. We found that,...
article 1982
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Kralj, S. (author), van Geel-Schutten, G.H. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
Lactobacillus reuteri strain 121 uses sucrose for synthesis of a unique, soluble glucan ('reuteran') with mainly α-(1→4) glucosidic linkages. The gene (gtfA) encoding this glucansucrase enzyme had previously been characterized. Here, a detailed biochemical and molecular analysis of the GTFA enzyme is presented. This is believed to be the first...
article 2004
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Koopman, J. (author), Haverkate, F. (author), Grimbergen, J. (author), Lord, S.T. (author), Mosesson, M.W. (author), DiOrio, J.P. (author), Siebenlist, K.S. (author), Legrand, C. (author), Soria, J. (author), Soria, C. (author), Caen, J.P. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
The molecular defect in the abnormal fibrinogen Dusart (Paris V) that is associated with thrombophilia was determined by sequence analysis of genomic DNA that had been amplified using the polymerase chain reaction. The propositus was heterozygous for a single base change (C → T) in the Aα- chain gene, resulting in the amino acid substitution Aα...
article 1993
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Noort, D. (author), Benschop, H.P. (author), Black, R.M. (author), Prins Maurits Laboratorium TNO (author)
In this report an overview of the methods currently available for detection of exposure to a number of chemical warfare agents (CWA), i.e., sulfur mustard, lewisite and nerve agents, is presented. Such methods can be applied for various purposes, e.g., diagnosis and dosimetry of exposure of casualties, confirmation of nonexposure, verification...
article 2002
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Hissink, A.M. (author), Oudshoorn, M.J. (author), van Ommen, B. (author), Haenen, G.R.M.M. (author), van Bladeren, P.J. (author)
The oxidative biotransformation of 1, 2-dichlorobenzene (1, 2-DCB) was investigated using hepatic microsomes from male Wistar, Fischer-344 and Sprague-Dawley (SD) rats, phenobarbital (PB)- and isoniazid (ISO) pretreated male Wistar rats and from man. In addition, microsomes from cell lines selectively expressing one cytochrome P450 (P4502E1, 1A1...
article 1996
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Verwei, M. (author), Arkbåge, K. (author), Mocking, H. (author), Havenaar, R. (author), Groten, J. (author), TNO Voeding (author)
Despite its low natural folate concentration, milk is responsible for 10-15% of the daily folate intake in countries with a high dairy consumption. Milk products can be considered as a potential matrix for folate fortification, e.g., with synthetic folic acid, to enhance the daily intake of folate. In untreated milk, the natural folate, 5...
article 2004
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End, C. (author), Bikker, F.J. (author), Renner, M. (author), Bergmann, G. (author), Lyer, S. (author), Blaich, S. (author), Hudler, M. (author), Helmke, B. (author), Gassler, N. (author), Autschbach, F. (author), Ligtenberg, A.J.M. (author), Benner, A. (author), Holmskov, U. (author), Schirmacher, P. (author), Nieuw Amerongen, A.V. (author), Rosenstiel, P. (author), Sina, C. (author), Franke, A. (author), Hafner, M. (author), Kioschis, P. (author), Schreiber, S. (author), Poustka, A. (author), Mollenhauer, J. (author)
Deleted in malignant brain tumors 1 (DMBT1) is a secreted glycoprotein displaying a broad bacterial-binding spectrum. Recent functional and genetic studies linked DMBT1 to the suppression of LPS-induced TLR4-mediated NF-kappaB activation and to the pathogenesis of Crohn's disease. Here, we aimed at unraveling the molecular basis of its function...
article 2009
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Jantos, R. (author), Schuhmacher, M. (author), Veldstra, J.L. (author), Bosker, W.M. (author), Klöpping-Ketelaars, I. (author), Touliou, K. (author), Sardi, G.M. (author), Brookhuis, K.A. (author), Ramaekers, J.G. (author), Mattern, R. (author), Skopp, G. (author)
For forensic toxicological investigations only whole blood, but no serum is often available. Pharmacokinetic data are helpful for interpreting the results, but most of these studies indicate serum or plasma concentrations. In order to obtain reliable conversion factors which also take intersubject variability into account, the blood/serum ratios...
article 2011
Searched for: subject:"Protein%5C+Binding"
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