Searched for: subject:"Protein%5C%2BBinding"
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Hissink, A.M. (author), Oudshoorn, M.J. (author), van Ommen, B. (author), Haenen, G.R.M.M. (author), van Bladeren, P.J. (author)
The oxidative biotransformation of 1, 2-dichlorobenzene (1, 2-DCB) was investigated using hepatic microsomes from male Wistar, Fischer-344 and Sprague-Dawley (SD) rats, phenobarbital (PB)- and isoniazid (ISO) pretreated male Wistar rats and from man. In addition, microsomes from cell lines selectively expressing one cytochrome P450 (P4502E1, 1A1...
article 1996
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Sakharov, D.V. (author), Lijnen, H.R. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
Staphylokinase (STA), a protein of bacterial origin, induces highly fibrin-specific thrombolysis both in human plasma in vitro and in pilot clinical trials. Using fluorescence microscopy, we investigated the spatial distribution of fluorescein isothiocyanate (FITC)-labeled STA during lysis of a plasma clot and its binding to purified fibrin...
article 1996
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Rijken, D.C. (author), Jie, A.F.H. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)
Reteplase is a protein consisting of the kringle-2 and protease domains of tissue-type plasminogen activator (t-PA). Because intravenous heparin will be used as an adjunct to thrombolytic therapy with reteplase, we investigated the interactions in vitro between heparin and reteplase as well as between heparin and recombinant t-PA (alteplase) as...
article 1996
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de Man, F.H.A.F. (author), de Beer, F. (author), van der Laarse, A. (author), Smelt, A.H.M. (author), Havekes, L.M. (author), Gaubius Instituut TNO (author)
An in vitro assay to study lipolysis of very low density lipoproteins (VLDL) by heparan sulfate proteoglycan (HSPG-bound lipoprotein lipase (LPL) was developed. Optimal conditions for VLDL lipolysis by HSPG-bound LPL were obtained by incubating plastic wells with 0.5 μg HSPG and 1.5 μg LPL, subsequently. Control experiments with heparinase...
article 1997
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Noorman, F. (author), Barrett-Bergshoeff, M.M. (author), Bekkers, M. (author), Emeis, J.J. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
Dextran is used during surgery as a prophylactic agent to prevent deep venous thrombosis. Recently it has been shown that dextran increases t-PA plasma concentrations in patients. As dextran is a potential ligand for the mannose receptor, we studied whether this glucose-polymer would be able to inhibit mannose receptor-mediated clearance of t-PA...
article 1997
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Mulder, M. (author), Kohnert, U. (author), Fischer, S. (author), van Hinsbergh, V.W.M. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)
The Escherichia coli-expressed recombinant plasminogen activator (r-PA) comprising the kringle 2 and protease domains of human tissue-type plasminogen activator (t-PA) has a four-fold longer half-life time in the circulation than t-PA, possibly resulting in an increased opportunity for r-PA to interact with the endothelial lining. In the present...
article 1997
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Laman, J.D. (author), van Meurs, M. (author), de Smet, B.J.G.L. (author), Schoneveld, A. (author), TNO Preventie en Gezondheid (author)
The abundant presence of macrophages and activated T cells in atherosclerotic plaques suggests an active involvement of the immune system in this cardiovascular disease. However, insight into cellular interactions inducing relevant effector functions is still limited. Here, Jon Laman and colleagues propose that local interactions between CD40...
article 1997
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Koopman, J.L. (author), Slomp, J. (author), de Bart, A.C.W. (author), Quax, P.H.A. (author), Verheijent, J.H. (author), Gaubius Instituut TNO (author)
The structural and functional properties of the urokinase-type plasminogen activator (u-PA) that are involved in the mitogenic effect of this proteolytic enzyme on human melanoma cells M14 and IF6 and the role of the u-PA receptor (u-PAR) in transducing this signal were analyzed. Native u- PA purified from urine induced a mitogenic response in...
article 1998
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Gaubius instituut TNO (author), Jong, M.C. (author), Willems-van Dijk, K. (author), Dahlmans, V.E.H. (author), van der Boom, H. (author), Kobayashi, K. (author), Oka, K. (author), Siest, G. (author), Chan, L. (author), Hofker, M.H. (author), Havekes, L.M. (author)
We have shown previously that human apolipoprotein (apo)Cl transgenic mice exhibit hyperlipidaemia, due primarily to an impaired clearance of very-low-density lipoprotein (VLDL) particles from the circulation. In the absence of at least the low-density-lipoprotein receptor (LDLR), it was shown that APOCI overexpression in transgenic mice...
article 1999
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Noort, D. (author), Hulst, A.G. (author), Fidder, A. (author), van Gurp, R.A. (author), de Jong, L.P.A. (author), Benschop, H.P. (author), Prins Maurits Laboratorium TNO (author)
The development of procedures for retrospective detection and quantitation of exposure to phosgene, based on adducts to hemoglobin and albumin, is described. Upon incubation of human blood with [14C]phosgene (0-750 μM), a significant part of radioactivity (0-13%) became associated with globin and albumin. Upon Pronase digestion of globin, one of...
article 2000
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Albertini, R.J. (author), Anderson, D. (author), Douglas, G.R. (author), Hagmar, L. (author), Hemminki, K. (author), Merlo, F. (author), Natarajan, A.T. (author), Norppa, H. (author), Shuker, D.E.G. (author), Tice, R. (author), Waters, M.D. (author), Aitio, A. (author), TNO Kwaliteit van Leven TNO Bibra (author)
The purpose of these guidelines is to provide concise guidance on the planning, performing and interpretation of studies to monitor groups or individuals exposed to genotoxic agents. Most human carcinogens are genotoxic but not all genotoxic agents have been shown to be carcinogenic in humans. Although the main interest in these studies is due...
article 2000
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Fidder, A. (author), Noort, D. (author), Hulst, A.G. (author), de Jong, L.P.A. (author), Benschop, H.P. (author), Prins Maurits Laboratorium TNO (author)
The development of a procedure for retrospective detection and quantitation of exposure to the arsenical dichloro(2-chlorovinyl)arsine (lewisite; L1) has been initiated. Upon incubation of human blood with [14C]L1 (20 nM-0.2 mM) in vitro, more than 90% of the total radioactivity was found in the erythrocytes and 25-50% of the radioactivity...
article 2000
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van Nieuw Amerongen, G.P. (author), Delft, S.V. (author), Vermeer, M.A. (author), Collard, J.G. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Endothelial cells (ECs) actively regulate the extravasation of blood constituents. On stimulation by vasoactive agents and thrombin, ECs change their cytoskeletal architecture and small gaps are formed between neighboring cells. These changes partly depend on a rise in [Ca2+](i) and activation of the Ca2+/calmodulin-dependent myosin light chain...
article 2000
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Noort, D. (author), Hulst, A.G. (author), Jansen, R. (author), Prins Maurits Laboratorium TNO (author)
Covalent binding of various clinically important nitrogen mustards to the cysteine-34 residue of human serum albumin, in vitro and in vivo, is demonstrated. A rapid method for detection of these adducts is presented, based on liquid chromatography-tandem mass spectrometry analysis of the adducted tripeptide Cys*-Pro-Phe after digestion of the...
article 2002
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van der Schans, G.P. (author), Noort, D. (author), Mars-Groenendijk, R.H. (author), Fidder, A. (author), Chau, L.F. (author), de Jong, L.P.A. (author), Benschop, H.P. (author), Prins Maurits Laboratorium TNO (author)
As part of a program to develop methods for diagnosis of exposure to chemical warfare agents, we developed immunochemical methods for detection of adducts of sulfur mustard to keratin in human skin. Three partial sequences of keratins containing glutamine or asparagine adducted with a 2-hydroxyethylthioethyl group at the ω-amide function were...
article 2002
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Noort, D. (author), Benschop, H.P. (author), Black, R.M. (author), Prins Maurits Laboratorium TNO (author)
In this report an overview of the methods currently available for detection of exposure to a number of chemical warfare agents (CWA), i.e., sulfur mustard, lewisite and nerve agents, is presented. Such methods can be applied for various purposes, e.g., diagnosis and dosimetry of exposure of casualties, confirmation of nonexposure, verification...
article 2002
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Walker, J.B. (author), Hughes, B. (author), James, I. (author), Haddock, P. (author), Kluft, C. (author), Bajzar, L. (author), Gaubius Instituut TNO (author)
Two competitive inhibitors of TAFIa (activated thrombin-activable fibrinolysis inhibitor), 2-guanidinoethyl-mercaptosuccinic acid and potato tuber carboxypeptidase inhibitor, variably affect fibrinolysis of clotted human plasma. Depending on their concentration, the inhibitors shortened, prolonged, or had no effect on lysis in vitro. The...
article 2003
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Nguyen, M.T. (author), Beck, J. (author), Lue, H. (author), Fünfzig, H. (author), Kleemann, R. (author), Koolwijk, P. (author), Kapurniotu, A. (author), Bernhagen, J. (author), Gaubius Instituut TNO (author)
Macrophage migration inhibitory factor (MIF) is a cytokine that participates in the host inflammatory response. A Cys-Xaa-Xaa-Cys (CXXC)-based thiol-protein oxidoreductase activity of MIF is associated with certain biological functions. Peptides spanning the CXXC region of thiol-protein oxidoreductases retain some biochemical properties of the...
article 2003
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Kleemann, R. (author), Gervois, P.P. (author), Verschuren, L. (author), Staels, B. (author), Princen, H.M.G. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)
C-reactive protein (CRP) is a major acute-phase protein in humans. Elevated plasma CRP levels are a risk factor for cardiovascular disease. CRP is predominantly expressed in hepatocytes and is induced by interleukin-1 (IL-1) and IL-6 under inflammatory situations, such as the acute phase. Fibrates are hypolipidemic drugs that act through the...
article 2003
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Verwei, M. (author), Arkbåge, K. (author), Mocking, H. (author), Havenaar, R. (author), Groten, J. (author), TNO Voeding (author)
Despite its low natural folate concentration, milk is responsible for 10-15% of the daily folate intake in countries with a high dairy consumption. Milk products can be considered as a potential matrix for folate fortification, e.g., with synthetic folic acid, to enhance the daily intake of folate. In untreated milk, the natural folate, 5...
article 2004
Searched for: subject:"Protein%5C%2BBinding"
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