Tissue factor pathway inhibitor in endothelial cells colocalizes with glycolipid microdomains/caveolae: Regulatory mechanism(s) of the anticoagulant properties of the endothelium

Tissue factor pathway inhibitor (TFPI), the main downregulator of the procoagulant activity of tissue factor·factor VIIa complex, locates in human endothelial cells (EC) in culture as well-defined clusters uniformly distributed both on the cell surface and intracellularly. We here demonstrate by immunofluorescence that TFPI colocalizes in EC...

The influence of α-galactosidase supplementation on the ileal digestibility of lupin seed carbohydrates and dietary protein in young pigs

Eighteen cannulated piglets were used in two experiments (six in Experiment 1 and 12 in Experiment 2) to determine the effect of supplementation of the diet with microbial α-galactosidase on the ileal digestibility of the raffinose family oligosaccharides and other nutrients of lupin-based diets. Three semi-purified diets with the seed meal of...

TEMPO-mediated oxidation of pullulan and influence of ionic strength and linear charge density on the dimensions of the obtained polyelectrolyte chains

The applied pH during the TEMPO-mediated (2,2,6,6-tetramethylpiperidine-1-oxyl) oxidation of the polysaccharide pullulan has a substantial influence on the molar mass distribution of the products. The least degradation was found in the pH range 9.2-9.7 and especially at higher pH, significant degradation occurs due to β-elimination. Four samples...

Low density lipoprotein receptor internalizes low density and very low density lipoproteins that are bound to heparan sulfate proteoglycans via lipoprotein lipase

It has previously been shown that lipoprotein lipase (LPL) enhances the binding of low density lipoproteins (LDL) and very low density lipoproteins (VLDL) to HepG2 cells and fibroblasts, up to 80-fold. This increase in binding is LDL receptor-independent and is due to a bridging of LPL between extracellular heparan sulfate proteoglycans (HSPG)...

The role of fungal polysaccharidases in the hydrolysis of cell wall materials from sunflower and palm-kernel meals

Main fractions from multi-component polysaccharidase preparations (Driselase, Gamanase and an experimental preparation of fungal origin), previously used for the enzymic treatment of cell wall materials from sunflower and palm-kernel meals, were sub-fractionated by different chromatographic techniques to evaluate the contribution of each of...

Nutritional implications of D-xylose in pigs

Hemicellulose consists primarily of pentose sugars, joined together in a polysaccharide chain with D-xylose as the most abundant component. Ileal digestibility and urinary excretion of D-xylose and associated effects of this pentose sugar on ileal and faecal digestibility of dry matter (DM), organic matter (OM), gross energy (GE) and nitrogen...