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TNO Preventie en Gezondheid (author), Bayley, J.P. (author), Bakker, A.M. (author), Kaijzel, E.L. (author), Wierenga, E.A. (author), van der Pouw-Kraan, T.C.T.M. (author), Huizinga, T.W.J. (author), Verweij, C.L. (author)The occurrence of monoallelic expression of cytokine genes in single cells has been convincingly demonstrated, but there have been few reports of this phenomenon in T cell clones. Here we describe studies on the expression of alleles of the human genes encoding IL-2, IL-3, IL-4, and IL-13 in human CD4+ T cell clones. In contrast to the results...article 2003
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Sierksma, A. (author), van der Gaag, M.S. (author), van Tol, A. (author), James, R.W. (author), Hendriks, H.F.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Background: The inverse association between moderate drinking and coronary heart disease mortality is well established. This study was performed to investigate the kinetics of the alcohol-induced increases in apo A-1, HDL cholesterol, and paraoxonase (PON) activity, as well as to study whether the alcohol-induced increases in PON activity differ...article 2002
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Gaubius Instituut TNO (author), Heijmans, B.T. (author), Westendorp, R.G.J. (author), Droog, S. (author), Kluft, C. (author), Knook, D.L. (author), Slagboom, P.E. (author)Ample evidence supports a role for tumour necrosis factor α (TNFα) in the development of type 2 diabetes and cardiovascular disease. TNFα expression was found to be influenced by a -308G/A polymorphism in the promoter of the gene encoding TNFα (TNF). We investigated the contribution of this polymorphism to diabetes and cardiovascular mortality...article 2002
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van Delft, J.H.M. (author), Steenwinkel, M.-J.S.T. (author), van Asten, J.G. (author), de Vogel, N. (author), Bruijntjes-Rozier, T.C.D.M. (author), Schouten, T. (author), Cramers, P. (author), Maas, L. (author), van Herwijnen, M.H. (author), van Schooten, F.-J. (author), Hopmans, P.M.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Occupational exposure to polycyclic aromatic hydrocarbons (PAH) increases the risk of developing lung cancer. Human exposure is often demonstrated by increased internal levels of PAH metabolites and of markers for early biological effects, like DNA adducts and cytogenetic aberrations. Objective: This study aimed to assess whether the current...article 2001
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de Boer, P. (author), Duim, B. (author), Rigter, A. (author), van der Plas, J. (author), Jacobs-Reitsma, W.F. (author), Wagenaar, J.A. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)For epidemiological tracing of the thermotolerant Campylobacter species C. jejuni and C. coli, reliable and highly discriminatory typing techniques are necessary. In this study the genotyping techniques of flagellin typing (flaA typing), pulsed-field gel electrophoresis (PFGE), automated ribotyping, and amplified fragment length polymorphism ...article 2000
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Gaubius Instituut TNO Preventie en Gezondheid (author), Neale, M.C. (author), de Knijff, P. (author), Havekes, L.M. (author), Boomsma, D.I. (author)ApoE levels and chromosome 19 ApoE polymorphisms were measured in a sample of 156 Dutch families. Each pedigree consisted of parents aged 35-65 years and their twin offspring aged 14-21 years. A significant effect of the chromosome 19 apoE locus on quantitative plasma levels of apolipoprotein E was observed. The ApoE polymorphism explained 16%...article 2000
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Common paraoxonase gene variants, mortality risk and fatal cardiovascular events in elderly subjectsGaubius Instituut TNO (author), Heijmans, B.T. (author), Westendorp, R.G.J. (author), Lagaay, A.M. (author), Knook, D.L. (author), Kluft, C. (author), Slagboom, P.E. (author)Recent studies indicate that the enzyme paraoxonase may be an important modulator of cardiovascular disease risk because of its ability to protect LDL from oxidation. We tested for association between two functional variants of the paraoxonase gene (Met-55/Leu and Gln-192/Arg) and both all-cause mortality and fatal cardiovascular disease. This...article 2000
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Zhang, B. (author), Ye, S. (author), Herrmann, S.M. (author), Eriksson, P. (author), de Maat, M. (author), Evans, A. (author), Arveiler, D. (author), Luc, G. (author), Cambien, F. (author), Hamsten, A. (author), Watkins, H. (author), Henney, A.M. (author), TNO Preventie en Gezondheid (author)Background - Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. This study tested the hypothesis that sequence variation in the promoter region of the...article 1999
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Gaubius instituut TNO (author), de Maat, M.P.M. (author), Jukema, J.W. (author), Ye, S. (author), Zwinderman, A.H. (author), Moghaddam, P.H. (author), Beekman, M. (author), Kastelein, J.J.P. (author), van Boven, A.J. (author), Bruschke, A.V.G. (author), Humphries, S.E. (author), Kluft, C. (author), Henney, A.M. (author)It has proved difficult to identify high-risk patients for atherosclerosis and to determine how they might respond to medication. Recently, a common promoter variant of the human stromelysin-1 gene has been reported, which has been shown to affect the transcription. We investigated whether this polymorphism had any impact on the risk of events,...article 1999
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Gaubius instituut TNO (author), Westendorp, R.G.J. (author), Hottenga, J.J. (author), Slagboom, P.E. (author)Background. Some patients infected with Neisseria meningitidis develop septic shock accompanied by fibrin deposition and microthrombus formation in various organs, whereas others develop bacteraemia or meningitis without septic shock. We investigated whether genetic differences in the fibrinolytic system influence the development of...article 1999
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Mennen, L.I. (author), de Maat, M.P.M. (author), Schouten, E.G. (author), Kluft, C. (author), Witteman, J.C.M. (author), Hofman, A. (author), Grobbee, D.E. (author), Gaubius Instituut TNO (author)The objective of this study was to evaluate the association of factor VII with dietary factors while also considering the R/Q353 polymorphism. Nutrition is an important determinant of coagulation factor VII, which is also genetically determined by the R/Q353 polymorphism. High levels of coagulation factor VII clotting activity (FVII:C) are...article 1998
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de Maat, M.P.M. (author), Kastelein, J.J.P. (author), Jukema, J.W. (author), Zwinderman, A.H. (author), Jansen, H. (author), Groenemeier, B. (author), Bruschke, A.V.G. (author), Kluft, C. (author), Gaubius instituut TNO (author)Increased plasma fibrinogen levels have been identified as a risk indicator for myocardial infarction, stroke, and thrombosis. Both environmental and genetic factors make an important contribution to plasma fibrinogen levels in humans. In the present study we evaluated, in patients with serum cholesterol levels between 4 and 8 mmol/L, the...article 1998
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Boer, J.M.A. (author), Feskens, E.J.M. (author), Schouten, E.G. (author), Havekes, L.M. (author), Seidell, J.C. (author), Kromhout, D. (author), Gaubius instituut TNO (author)To elucidate the role of modifiable factors and the apolipoprotein E polymorphism in explaining lipid profiles reflecting low, average and high risk for coronary heart disease, we selected subjects from a large population-based study. Subjects with low total cholesterol (TC) (< 15th percentile) and high HDL-cholesterol levels (> 85th percentile)...article 1998
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Di Castelnuovo, A. (author), D'Orazio, A. (author), Amore, C. (author), Falanga, A. (author), Kluft, C. (author), Donati, M.B. (author), Lacoviello, L. (author), Gaubius Instituut TNO (author)We studied the relationships among different polymorphisms of FVII gene in determining FVII levels, in a sample of 335 male and female Italian volunteers. The hypervariable region 4 (HVR4), the promoter decanucleotide insertion (-323 0/10 bp) and the R353Q polymorphisms of FVII gene were evaluated. The association of HVR4 or -323 0/10 bp...article 1998
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Ferrer-Antunes, C. (author), de Maat, M.P.M. (author), Palmeiro, A. (author), Pimentel, J. (author), Fernandes, V. (author), Gaubius Instituut TNO (author)Fibrinogen is an acute phase reactant, and there, fore its plasma levels increase after severe injury. Polymorphisms in the fibrinogen α and β genes have been found to be associated with plasma levels of fibrinogen, and it has also been suggested that they are associated with the fibrinogen increase in acute phase situations. In forty-five...article 1998
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van der Bom, J.G. (author), de Maat, M.P.M. (author), Bots, M.L. (author), Haverkate, F. (author), de Jong, P.T.V.M. (author), Hofman, A. (author), Kluft, C. (author), Grobbee, D.E. (author), Gaubius Instituut TNO (author)An association between increased plasma fibrinogen and an increased risk for myocardial infarction (MI) is well established, but the nature of this association is subject to debate. Our aim was to shed light on the potentially causal nature of this association. We examined whether increased plasma fibrinogen, due to a condition that is...article 1998
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Zito, F. (author), di Castelnuovo, A. (author), Amore, C. (author), D'Orazio, A. (author), Donati, M.B. (author), Iacoviello, L. (author), Gaubius Instituut TNO (author)The aim of this study was to investigate the association of the Bcl I β-chain fibrinogen polymorphism with the risk of acute myocardial infarction (AMI) and its relationship with fibrinogen levels in the Italian population. We studied 102 AMI patients, selected within the framework of the GISSI-2 trial, who had a familial history of arterial...article 1997
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Boer, J.M.A. (author), Ehnholm, C. (author), Menzel, H.J. (author), Havekes, L.M. (author), Rosseneu, M. (author), O'Reilly, D.S.J. (author), Tiret, L. (author), Gaubius Instituut TNO (author)To elucidate how the apolipoprotein (apo)E polymorphism and modifiable factors interact in explaining plasma lipid and apolipoprotein levels, we studied 1448 young adults (18 to 26 years old), participating in the European Atherosclerosis Research Study (EARS). Venous blood was collected after an overnight fast. Modifiable factors, eg, body mass...article 1997
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- Dell' Acqua, G. (author), Iacoviello, L. (author), D' Orazio, A. (author), Di Bitondo, R. (author), Di Castelnuovo, A. (author), Donati, M.B. (author), Gaubius Instituut TNO (author) article 1997
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van der Bom, J.G. (author), de Knijff, P. (author), Haverkate, F. (author), Bots, M.L. (author), Meijer, P. (author), de Jong, P.T.V.M. (author), Hofman, A. (author), Kluft, C. (author), Grobbee, D.E. (author), Gaubius Instituut TNO (author)Background: Impaired fibrinolytic capacity, as assessed by euglobulin clot lysis time or plasma concentration of fibrinolytic parameters, has been associated with an increased risk of myocardial infarction (MI). We studied the association of a polymorphism in the gene for TPA and of plasma concentrations of TPA (antigen and activity) with the...article 1997