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Effect of steroid hormones and retinoids on the formation of capillary-like tubular structures of human microvascular endothelial cells in fibrin matrices is related to urokinase expression

Lansink, M. (author), Koolwijk, P. (author), van Hinsbergh, V. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)

Angiogenesis, the formation of new capillary blood vessels, is a feature of a variety of pathological processes. To study the effects of a specific group of hormones (all ligands of the steroid/retinoid/thyroid hormone receptor superfamily) on the angiogenic process in humans, we have used a model system in which human microvascular endothelial...

article 1998

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Immunoglobulin V(H) gene sequence analysis of spontaneous murine immunoglobulin secreting B-cell tumours with clinical features of human disease

Zhu, D. (author), van Arkel, C. (author), King, C.A. (author), van Meirvenne, S. (author), de Greef, C. (author), Thielemans, K. (author), Radl, J. (author), Stevenson, F.K. (author), TNO Preventie en Gezondheid (author)

The 5T series of multiple myelomas (MM) and Waldenstrsom's macroglobulinaemia-like lymphomas (WM), which developed spontaneously in ageing mice of the C57BL/KaLwRij strain, shows clinical and biological features that closely resemble their corresponding human diseases. In order to compare the patterns of somatic mutation in V(H) genes of mouse...

article 1998

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Dietary effects on coagulation factor VII vary across genotypes of the R/Q353 polymorphism in elderly people

Mennen, L.I. (author), de Maat, M.P.M. (author), Schouten, E.G. (author), Kluft, C. (author), Witteman, J.C.M. (author), Hofman, A. (author), Grobbee, D.E. (author), Gaubius Instituut TNO (author)

The objective of this study was to evaluate the association of factor VII with dietary factors while also considering the R/Q353 polymorphism. Nutrition is an important determinant of coagulation factor VII, which is also genetically determined by the R/Q353 polymorphism. High levels of coagulation factor VII clotting activity (FVII:C) are...

article 1998

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Molecular cloning and functional expression in Lactobacillus plantarum 80 of xylT, encoding the D-xylose-H+ symporter of Lactobacillus brevis

Chaillou, S. (author), Bor, Y.-C. (author), Batt, C.A. (author), Postma, P.W. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

A 3-kb region, located downstream of the Lactobacillus brevis xylA gene (encoding D-xylose isomerase), was cloned in Escherichia coli TG1. The sequence revealed two open reading frames which could code for the D-xylulose kinase gene (xylB) and another gene (xylT) encoding a protein of 457 amino acids with significant similarity to the D-xylose-H...

article 1998

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Reduction of campylobacter infections in broiler flocks by application of hygiene measures

van de Giessen, A.W. (author), Tilburg, J.J.H.C. (author), Ritmeester, W.S. (author), van der Plas, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

Transmission routes of Campylobacter spp. in broilers and possibilities for prevention of infections were studied on two Dutch broiler farms. The occurrence of Campylobacter spp. was studied in successive broiler flocks, in the environment of the farms and in some of the parent flocks involved. Isolates of Campylobacter spp. were typed by using...

article 1998

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An improved colony-PCR method for filamentous fungi for amplification of pcr-fragments of several kilobases

van Zeijl, C.M.J. (author), van de Kamp, E.H.M. (author), Punt, P.J. (author), Selten, G.C.M. (author), Hauer, B. (author), van Gorcom, R.F.M. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

A method was developed to perform PCR directly on mycelial pellets or colonies treated with NOVOzym 234. The method allows rapid screening of large numbers of transformants of both sporulating and non-sporulating fungi for the presence of (co)transforming plasmid copies or for specific genetic modifications such as gene disruption and site...

article 1998

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Matrix metalloproteinase (MMP)-9 type IV collagenase/gelatinase implicated in the pathogenesis of Sjogren's syndrome

Konttinen, Y.T. (author), Halinen, S. (author), Hanemaaijer, R. (author), Sorsa, T. (author), Hietanen, J. (author), Ceponis, A. (author), Xu, J.W. (author), Manthorpe, R. (author), Whittington, J. (author), Larsson, Å. (author), Salo, T. (author), Kjeldsen, L. (author), Stenman, U.-H. (author), Eisen, A.Z. (author), Gaubius Instituut TNO (author)

Type IV collagenases/gelatinases (matrix metalloproteinases MMP-2 and MMP-9) in labial salivary glands (LSG) and saliva in Sjogren's syndrome (SS) and healthy controls were studied. Zymograms and Western blots disclosed that SS saliva contained 92/82 kD MMP-9/type IV collagenase duplex. Specific activity measurement disclosed 53.1 ± 9.8 U/mg...

article 1998

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Elevated plasma fibrinogen: Cause or consequence of cardiovascular disease?

van der Bom, J.G. (author), de Maat, M.P.M. (author), Bots, M.L. (author), Haverkate, F. (author), de Jong, P.T.V.M. (author), Hofman, A. (author), Kluft, C. (author), Grobbee, D.E. (author), Gaubius Instituut TNO (author)

An association between increased plasma fibrinogen and an increased risk for myocardial infarction (MI) is well established, but the nature of this association is subject to debate. Our aim was to shed light on the potentially causal nature of this association. We examined whether increased plasma fibrinogen, due to a condition that is...

article 1998

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Cell-type specific DNA-protein interactions at the tissue-type plasminogen activator promoter in human endothelial and HeLa cells in vivo and in vitro

Arts, J. (author), Herr, I. (author), Lansink, M. (author), Angel, P. (author), Kooistra, T. (author), TNO Preventie en Gezondheid (author)

Tissue-type plasminogen activator (t-PA) gene expression in human endothelial cells and HeLa cells is stimulated by the protein kinase C activator phorbol 12-myristate 13-acetate (PMA) at the level of transcription. To study the mechanism of transcriptional regulation, we have characterized a segment of the t-PA gene extending from -135 to +100...

article 1997

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A polymorphic cluster in the 5' region of the human coagulation factor VII gene: Detection, frequency, and linkage disequilibrium

Dell' Acqua, G. (author), Iacoviello, L. (author), D' Orazio, A. (author), Di Bitondo, R. (author), Di Castelnuovo, A. (author), Donati, M.B. (author), Gaubius Instituut TNO (author)

article 1997

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Formation and persistence of O6-ethylguanine in genomic and transgene DNA in liver and brain of λlacZ transgenic mice treated with N-ethyl-N-nitrosourea

Mientjes, E.J. (author), Hochleitner, K. (author), Luiten-Schuite, A. (author), van Delft, J.H.M. (author), Thomale, J. (author), Berends, F. (author), Rajewsky, M.F. (author), Lohman, P.H.M. (author), Baan, R.A. (author), TNO Voeding (author)

LacZ transgenic mice are suitable for short-term mutagenicity studies in vivo. Mutagenicity in these mice is determined in the lacZ transgene. Since the lacZ gene is of bacterial origin the question has been raised whether DNA-adduct formation and repair in the transgene are comparable to those in total genomic DNA. Mice were treated with N...

article 1996

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DNA based typing, identification and detection systems for food spoilage microorganisms: Development and implementation

van der Vossen, J.M.B.M. (author), Hofstra, H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

The rapid identification of spoilage microorganisms is of eminent importance to the food industry. It provides the food industry with the opportunity to reduce economical losses by designing adequate intervention measures. The use of identification systems based on biochemical and physiological characteristics resulted often in disappointing...

article 1996

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Interchange of the active and silent S-layer protein genes of Lactobacillus acidophilus by inversion of the chromosomal sip segment

Boot, H.J. (author), Kolen, C.P.A.M. (author), Pouwels, P.H. (author), Prins Maurits Laboratorium TNO (author)

The most-dominant surface-exposed protein in many bacterial species is the S-protein. This protein crystallises into a regular monolayer on the outside surface of the bacteria: the S-layer. Lactobacillus acidophilus harbours two S-protein-encoding genes, slpA and slpB, only one of which (slpA) is expressed. In this study, we show by polymerase...

article 1996

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Effects of verocytotoxin-1 on nonadherent human monocytes: Binding characteristics, protein synthesis, and induction of cytokine release

van Setten, P.A. (author), Monnens, L.A.H. (author), Verstraten, R.G.G. (author), van den Heuvel, L.P.W.J. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)

The epidemic form of the hemolytic uremic syndrome (HUS) has been associated with a verocytotoxin producing Escherichia coli infection. Endothelial cell damage of glomeruli and arterioles of the kidney plays a central role in the pathogenesis of HUS. A number of observations in vivo and in vitro indicate that inflammatory mediators contribute to...

article 1996

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Overexpression of transforming growth factor-α and epidermal growth factor receptor, but not epidermal growth factor, in exocrine pancreatic tumours in hamsters

Visser, C.J.T. (author), Bruggink, A.H. (author), Korc, M. (author), Kobrin, M.S. (author), de Weger, R.A. (author), Seifert-Bock, I. (author), van Blokland, W.T.M. (author), van Garderen Hoetmer, A. (author), Woutersen, R.A. (author), TNO Voeding (author)

Using immunohistochemistry, Northern blotting and a semi-quantitative PCR technique, epidermal growth factor (EGF), transforming growth factor-α (TGF-α) and epidermal growth factor receptor (EGFR) expression were studied in the pancreas of N-nitrosobis(2-oxopropyl)-amine (BOP)-treated hamsters. After initiation pancreatic carcinogenesis was...

article 1996

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Direct assessment of junctional diversity in rearranged T cell receptor β chain encoding genes by combined heteroduplex and single strand conformation polymorphism (SSCP) analysis

Offermans, M.T.C. (author), Struyk, L. (author), de Geus, B. (author), Breedveld, F.C. (author), van den Elsen, P.J. (author), Rozing, J. (author), TNO Preventie en Gezondheid (author)

In order to define the extent of T cell heterogeneity and clonality, unique DNA sequences in the junctional region in rearranged T cell receptor (TcR) genes can be studied. For this purpose we have adapted a non-denaturing nucleic acid gel electrophoresis procedure to detect TcR junctional diversity. Detection of junctional diversity is based...

article 1996

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Primary structure of a lipoxygenase from barley grain as deduced from its cDNA sequence

van Mechelen, J.R. (author), Smits, M. (author), Douma, A.C. (author), Rouster, J. (author), Cameron-Mills, V. (author), Heidekamp, F. (author), Valk, B.E. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

A full length cDNA sequence for a barley grain lipoxygenase was obtained. It includes a 5' untranslated region of 69 nucleotides, an open reading frame of 2586 nucleotides encoding a protein of 862 amino acid residues and a 3' untranslated region of 142 nucleotides. The molecular mass of the encoded polypeptide was calculated to be 96.392. Its...

article 1995

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Development of a detection system for histidine decarboxylating lactic acid bacteria based on DNA probes, PCR and activity test

Le Jeune, C. (author), Lonvaud-Funel, A. (author), ten Brink, B. (author), Hofstra, H. (author), van der Vossen, J.M.B.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

On the basis of the comparison of the nucleotide sequences of the histidine decarboxylase genes (hdcA) of Lactobacillus 30A and Clostridium perfringens and the amino acid sequences of these histidine decarboxylases and those of Lactobacillus buchneri and Micrococcus, oligonucleotides unique to the hdcA genes were synthesized and used in PCR. All...

article 1995

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Random amplified polymorphic DNA and restriction enzyme analysis of PCR amplified rDNA in taxonomy: Two identification techniques for food-borne yeasts

Baleiras Couto, M.M. (author), Vogels, J.T.W.E. (author), Hofstra, H. (author), Huis in 'tVeld, J.H.J. (author), van der Vossen, J.M.B.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

The random amplified polymorphic DNA (RAPD) assay and the restriction enzyme analysis of PCR amplified rDNA are compared for the identification of the common spoilage yeasts Zygosaccharomyces bailii, Z. rouxii, Saccharomyces cerevisiae, Candida valida and C. lipolytica. Both techniques proved to be adequate tools for yeast identification. Since...

article 1995

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Hepatocellular localisation of biosynthesis of vitronectin. Characterisation of the primary structure of rat vitronectin

Otter, M. (author), Kuiper, J. (author), Rijken, D. (author), van Zonneveld, A.-J. (author), TNO Preventie en Gezondheid (author)

To characterize the primary structure of rat Vn, a cDNA library constructed from freshly isolated rat hepatocytes, was screened with a human Vn cDNA probe. Comparison of the sequence of the obtained rat cDNA clone with the sequences of human, mouse and rabbit Vn cDNA's showed predominantly consensus in the somatomedin B domain, the RGD-sequence...

article 1995