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Eefting, D. (author), Seghers, L. (author), Grimbergen, J.M. (author), de Vries, M.R. (author), de Boer, H.C. (author), Lardenoye, J.W.H.P. (author), Jukema, J.W. (author), van Bockel, J.H. (author), Quax, P.H.A. (author), TNO Kwaliteit van Leven (author)Aims Matrix metalloproteinases (MMP) and plasminogen activator (PA)/plasmin-mediated proteolysis, especially at the cell surface, play important roles in matrix degeneration and smooth muscle cell migration, which largely contributes to vein graft failure. In this study, a novel hybrid protein was designed to inhibit both protease systems...article 2010
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Hawinkels, L.J.A.C. (author), Verspaget, H.W. (author), van der Reijden, J.J. (author), van der Zon, J.M. (author), Verheijen, J.H. (author), Hommes, D.W. (author), Lamers, C.B.H.W. (author), Sier, C.F.M. (author), TNO Kwaliteit van Leven (author)Transforming growth factor-β1 (TGF-β1), a cytokine involved in various stages of cancer, is produced as a latent complex and requires processing to become active. We have determined total and active TGF-β1 levels in homogenates of colorectal neoplasia. In contrast to total TGF-β levels, showing a stepwise increase in the mucosa-adenoma-carcinoma...article 2009
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Hellebrekers, B.W.J. (author), Trimbos-Kemper, T.C.M. (author), Boesten, L. (author), Jansen, F.W. (author), Kolkman, W. (author), Trimbos, J.B. (author), Press, R.R. (author), van Poelgeest, M.I.E. (author), Emeis, S.J. (author), Kooistra, T. (author), TNO Kwaliteit van Leven (author)Objective: To identify predictors of postsurgical adhesion formation in peritoneal fluid and plasma, and assess efficacy and safety of reteplase (recombinant plasminogen activator [r-PA]). Design: Prospective randomized study. Setting: University Medical Center. Patient(s): Twenty-six abdominal myomectomy patients with early second-look...article 2009
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Geert Heidema, A. (author), Thissen, U. (author), Boer, J.M.A. (author), Bouwman, F.G. (author), Feskens, E.J.M. (author), Mariman, E.C.M. (author), TNO Kwaliteit van Leven (author)In this study, we applied the multivariate statistical tool Partial Least Squares (PLS) to analyze the relative importance of 83 plasma proteins in relation to coronary heart disease (CHD) mortality and the intermediate end points body mass index, HDL-cholesterol and total cholesterol. From a Dutch monitoring project for cardiovascular disease...article 2009
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Rijken, D.C. (author), Hoegee-de Nobel, E. (author), Jie, A.F.H. (author), Atsma, D.E. (author), Schalij, M.J. (author), Nieuwenhuizen, W. (author), TNO Kwaliteit van Leven (author)Background: The development of global tests for the fibrinolytic capacity in blood is hampered by the low base-line fibrinolytic activity in blood, by the involvement of both plasmatic components and blood cells in the fibrinolytic system and by the loss of fibrinolytic activity as a result of the action of plasminogen activator inhibitor-1 (PAI...article 2008
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Alizadeh Dehnavi, R. (author), Beishuizen, E.D. (author), van de Ree, M.A. (author), Le Cessie, S. (author), Huisman, M.V. (author), Kluft, C. (author), Princen, H.M.G. (author), Tamsma, J.T. (author), TNO Kwaliteit van Leven (author)Background: The burden of cardiovascular disease in diabetes mellitus type 2 (DM2) patients is variable. We hypothesize that metabolic syndrome (MS) and low-grade systemic inflammation modify the extent of atherosclerosis in DM2. Methods: Vascular phenotype was determined using the following endothelium-related, hemostatic, and sonographic...article 2008
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- Hu, L. (author), Bovenschen, N. (author), Havekes, L.M. (author), van Vlijmen, B.J.M. (author), Tamsma, J.T. (author), TNO Kwaliteit van Leven (author) article 2007
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Spijkerman, A.M.W. (author), Gall, M.A. (author), Tarnow, L. (author), Twisk, J.W.R. (author), Lauritzen, E. (author), Lund-Andersen, H. (author), Emeis, J. (author), Parving, H.H. (author), Stehouwer, C.D.A. (author), TNO Kwaliteit van Leven (author)Aims: To study whether microalbuminuria, endothelial dysfunction and low-grade inflammation are associated with the presence and progression of diabetic retinopathy. Methods: Patients with Type 2 diabetes (n = 328) attending a diabetes clinic were followed for 10 years and examined annually during the last 7 years. Retinopathy was assessed after...article 2007
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Buscher, B.A.P. (author), Gerritsen, H. (author), van Schöll, I. (author), Cnubben, N.H.P. (author), Brüll, L.P. (author), TNO Kwaliteit van Leven (author)An LC-MS/MS method has been developed for the quantitative determination of a protein drug (Tenecteplase; MW 58,777 Da) in rat plasma. The protein was digested with trypsin without prior clean-up of the plasma sample, without the use of a label nor internal standard. A limited validation was performed to assess the linearity, the sensitivity and...article 2007
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TNO Kwaliteit van Leven (author), Pons, D. (author), Monraats, P.S. (author), de Maat, M.P.M. (author), Pires, N.M.M. (author), Quax, P.H.A. (author), van Vlijmen, B.J.M. (author), Rosendaal, F.R. (author), Zwinderman, A.H. (author), Doevendans, P.A.F.M. (author), Waltenberger, J. (author), de Winter, R.J. (author), Tio, R.A. (author), Frants, R.R. (author), van der Laarse, A. (author), van der Wall, E.E. (author), Jukema, J.W. (author)Since activation of the haemostatic system is an important feature of the wound healing response triggered by arterial injury, variations in genes involved in thrombus formation may play a role in restenosis after percutaneous coronary interventions (PCI). Therefore, our aim was to examine the relationship between polymorphisms that are known to...article 2007
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TNO Kwaliteit van Leven (author), Guimarães, A.H.C. (author), Laurens, N. (author), Weijers, E.M. (author), Koolwijk, P. (author), van Hinsbergh, V.W.M. (author), Rijken, D.C. (author)OBJECTIVE - Besides having a key role in fibrinolysis, the plasminogen system has been implicated in cell migration and angiogenesis. A common mechanism is the binding of plasminogen to carboxy-terminal lysine residues in partially degraded fibrin or on cellular surfaces. Here we examined the involvement of thrombin activatable fibrinolysis...article 2007
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de Lange, M. (author), de Geus, E.J.C. (author), Kluft, C. (author), Meijer, P. (author), van Doornen, L.J.P. (author), Boomsma, D.I. (author), Snieder, H. (author), TNO Kwaliteit van Leven (author)Differences in genetic influence on death from CHD between males and females have been reported. Haemostatic factors have consistently been associated with risk for coronary heart disease (CHD), but sex differences in genetic architecture have not been studied. This study in middle-aged twins investigates whether there are sex differences in...article 2006
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Rad, M. (author), Kluft, C. (author), Ménard, J. (author), Burggraaf, J. (author), de Kam, M.L. (author), Meijer, P. (author), Sivin, I. (author), Sitruk-Ware, R.L. (author), TNO Kwaliteit van Leven (author)Objective: This study aimed to compare the effects on hemostasis variables of a contraceptive vaginal ring with those of an oral contraceptive. Study design: Twenty-three and 22 healthy premenopausal women were randomized to the contraceptive vaginal ring (150 μg Nestorone and 15 μg ethinyl estradiol) or Stediril 30 during 3 cycles. Analysis of...article 2006
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Bertina, R.M. (author), van Tilburg, N.H. (author), Haverkate, F. (author), Bouma, B.N. (author), von dem Borne, P.A.K. (author), Meijers, J.C.M. (author), Campbell, W. (author), Eaton, D. (author), Hendriks, D.F. (author), Willemse, J.L. (author), TNO Kwaliteit van Leven (author)CAS: blood clotting factor 11, 9013-55-2; thrombin, 9002-04-4; tissue plasminogen activator, 105913-11-9; protein C, 60202-16-6; Carboxypeptidase U, 3.4.17.20; Protein C; Tissue Plasminogen Activator, 3.4.21.68article 2006
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TNO Kwaliteit van Leven TNO Preventie en Gezondheid (author), Maat, M.P.M.de (author), Bladbjerg, E.M. (author), Kluft, C. (author), Winkler, U.H. (author), Rekers, H. (author), Skouby, S.O. (author), Jespersen, J. (author)Background: Administration of oral contraceptives (OCs) has profound effects on the plasma levels of haemostasis and inflammation variables, resulting in an increased thrombosis risk. Individuals show large differences in the response of these variables to OCs. Polymorphism in the estrogen receptor-1 (ER1) gene may explain part of this inter...article 2006
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TNO Kwaliteit van Leven (author), Guimarães, A.H.C. (author), Barrett-Bergshoeff, M.M. (author), Criscuoli, M. (author), Evangelista, S. (author), Rijken, D.C. (author)In this study, the in-vitro fibrinolytic efficacy of Tenecteplase, Amediplase and scu-PA was investigated in different external lysis models by measuring the lysis of human plasma clots after the addition of the plasminogen activators (PAs) to the surrounding plasma. The effect of TAFI was examined for each PA by neutralising TAFIa with potato...article 2006
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Vogelvang, T.E. (author), Mijatovic, V. (author), Kenemans, P. (author), Emeis, J.J. (author), Heijst, J.A. (author), van der Mooren, M.J. (author), TNO Preventie en Gezondheid (author)Objective: To investigate the short-term effects of HMR 3339, a novel selective estrogen receptor modulator, on markers of coagulation and fibrinolysis. Study design: In a multicenter, 14-week, randomized, placebo-controlled, double-blind, dose-ranging study, healthy postmenopausal women received daily placebo (n = 22), HMR 3339 2.5 mg (n = 25),...article 2005
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Verschuren, L. (author), Kleemann, R. (author), Offerman, E.H. (author), Szalai, A.J. (author), Emeis, S.J. (author), Princen, H.M.G. (author), Kooistra, T. (author), TNO Kwaliteit van Leven (author)Objective - To evaluate whether low-dose atorvastatin suppresses atherosclerotic lesion progression and inflammation in apolipoprotein E*3 (apoE*3)-Leiden mice beyond its cholesterol-lowering effect. Methods and Results - ApoE*3-Leiden mice were fed a high-cholesterol (HC) diet until mild atherosclerotic lesions had formed. Subsequently, HC diet...article 2005
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Espirito Santo, S.M.S. (author), Pires, N.M.M. (author), Boesten, L.S.M. (author), Gerritsen, G. (author), Bovenschen, N. (author), van Dijk, K.W. (author), Jukema, J.W. (author), Princen, H.M.G. (author), Bensadoun, A. (author), Li, W.P. (author), Herz, J. (author), Havekes, L.M. (author), van Vlijmen, B.J.M. (author), Gaubius Instituut TNO (author)The low-density lipoprotein (LDL) receptor-related protein (LRP) has a well-established role in the hepatic removal of atherogenic apolipoprotein E (APOE)-rich remnant lipoproteins from plasma. In addition, LRP recognizes multiple distinct pro- and antiatherogenic ligands in vitro. Here, we investigated the role of hepatic LRP in atherogenesis...article 2004
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de Maat, M.P.M. (author), Jansen, M.W.J.C. (author), Hille, E.T.M. (author), Vos, H. (author), Bloemenkamp, K.W.M. (author), Buitendijk, S.E. (author), Helmerhorst, F.M. (author), Wladimiroff, J.W. (author), Bertina, R.M. (author), de Groot, C.J.M. (author), TNO Preventie en Gezondheid (author)Recently, it has been proposed that abnormalities in coagulation and fibrinolysis contribute to the development of preeclampsia by increasing the thrombotic tendency. This hypothesis was tested in women who have had preeclampsia (cases) compared with matched controls. Polymorphisms in the thrombophilia genes {plasminogen activator inhibitor type...article 2004