Searched for: subject%3A%22Phenotype%22
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Stierum, R. (author), Gaspari, M. (author), Dommels, Y. (author), Ouatas, T. (author), Pluk, H. (author), Jespersen, S. (author), Vogels, J. (author), Verhoeckx, K. (author), Groten, J. (author), van Ommen, B. (author)
Here, we describe a proteomics approach to study protein expression changes in differentiating Caco-2 cells. Caco-2 is a colorectal carcinoma cell line, which upon differentiation loses its tumorigenic phenotype and displays characteristics of mature enterocytes, including brush borders with microvilli. Cells were grown in culture flasks and...
article 2003
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Broekmans, W.M.R. (author), Vink, A.A. (author), Boelsma, E. (author), Klöpping-Ketelaars, W.A.A. (author), Tijburg, L.B.M. (author), van 't Veer, P. (author), van Poppel, G. (author), Kardinaal, A.F.M. (author), TNO Voeding (author)
Background: Acute effects of UV irradiation include UV-induced erythema. Sunlight plays an important role in the development of skin cancer. Several predictive factors of UV-induced erythema could also be predictive for skin cancer. Objective: Our objective was to quantitatively assess phenotypical and nutritional determinants of sensitivity to...
article 2003
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Berry, A. (author), Janssens, D. (author), Hümbelin, M. (author), Jore, J.P.M. (author), Hoste, B. (author), Cleenwerck, I. (author), Vancanneyt, M. (author), Bretzel, W. (author), Mayer, A.F. (author), Lopez-Ulibarri, R. (author), Shanmugam, B. (author), Swings, J. (author), Pasamontes, L. (author)
A comprehensive taxonomic re-evaluation was performed on the marine, zeaxanthin-producing bacterium formerly classified as [Flavobacterium] sp. strain R-1512 (ATCC 21588). This strain, together with two other previously described marine isolates, [Flavobacterium] strain R-1506 and Paracoccus sp. strain MBIC 3966, were found to comprise a new...
article 2003
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TNO Preventie en Gezondheid (author), Richters, C.D. (author), Mayen, I. (author), Havenith, C.E.G. (author), Beelen, R.H.J. (author), Kamperdijk, E.W.A. (author)
Dendritic cells (DC) are the most potent antigen-presenting cells and are therefore useful to induce immune responses against tumor cells in patients. DC can be generated in vitro from monocytes using GM-CSF and IL-4, the so-called monocyte-derived DC (MoDC). To achieve antitumor responses, MoDC must be able to migrate to the draining lymph...
article 2002
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TNO Preventie en Gezondheid (author), Raamsdonk, L.M. (author), Teusink, B. (author), Broadhurst, D. (author), Zhang, N. (author), Hayes, A. (author), Walsh, M.C. (author), Berden, J.A. (author), Brindle, K.M. (author), Kell, D.B. (author), Rowland, J.J. (author), Westerhoff, H.V. (author), van Dam, K. (author), Oliver, S.G. (author)
A large proportion of the 6,000 genes present in the genome of Saccharomyces cerevisiae, and of those sequenced in other organisms, encode proteins of unknown function. Many of these genes are "silent," that is, they show no overt phenotype, in terms of growth rate or other fluxes, when they are deleted from the genome. We demonstrate how the...
article 2001
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Bogaards, J.J.P. (author), Hissink, E.M. (author), Briggs, M. (author), Weaver, R. (author), Jochemsen, R. (author), Jackson, P. (author), Bertrand, M. (author), van Bladeren, P. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A strategy is presented to predict interindividual variation in drug plasma levels in vivo by the use of physiologically based pharmacokinetic modeling and human in vitro metabolic parameters, obtained through the combined use of microsomes containing single cytochrome P450 enzymes and a human liver microsome bank. The strategy, applied to the...
article 2000
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Gaubius Instituut TNO Preventie en Gezondheid (author), Neale, M.C. (author), de Knijff, P. (author), Havekes, L.M. (author), Boomsma, D.I. (author)
ApoE levels and chromosome 19 ApoE polymorphisms were measured in a sample of 156 Dutch families. Each pedigree consisted of parents aged 35-65 years and their twin offspring aged 14-21 years. A significant effect of the chromosome 19 apoE locus on quantitative plasma levels of apolipoprotein E was observed. The ApoE polymorphism explained 16%...
article 2000
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van der Kop, D.A.M. (author), Schuyer, M. (author), Pinas, J.E. (author), van der Zaal, B.J. (author), Hooykaas, P.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Transgenic arabidopsis plants were isolated that contained a T-DNA construct in which the promoter of an auxin-inducible glutathione S-transferase (GST) gene from tobacco was fused to the kanamycin resistance (nptII) as well as to the β-glucuronidase (gusA) reporter gene. Subsequently, seeds were treated with EMS to obtain mutants in which both...
article 1999
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Lutgens, E. (author), Daemen, M. (author), Kockx, M. (author), Doevendans, P. (author), Hofker, M. (author), Havekes, L. (author), Wellens, H. (author), de Muinck, E.D. (author), Gaubius instituut TNO (author)
Background - This study documents (1) the progression of atherosclerosis along the entire arterial tree in APOE(*)3-Leiden mice after 1, 4, 6, 9, and 12 months of a high-fat/high-cholesterol (HFC) diet and (2) the amount and phenotype of DNA-synthesizing and apoptotic cells in different lesion types after 6 months of HFC diet. Methods and...
article 1999
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Boer, J.M.A. (author), Feskens, E.J.M. (author), Schouten, E.G. (author), Havekes, L.M. (author), Seidell, J.C. (author), Kromhout, D. (author), Gaubius instituut TNO (author)
To elucidate the role of modifiable factors and the apolipoprotein E polymorphism in explaining lipid profiles reflecting low, average and high risk for coronary heart disease, we selected subjects from a large population-based study. Subjects with low total cholesterol (TC) (< 15th percentile) and high HDL-cholesterol levels (> 85th percentile)...
article 1998
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Schmand, B. (author), Hooijer, C. (author), Jonker, C. (author), Lindeboom, J. (author), Havekes, L.M. (author), Gaubius instituut TNO (author)
Apolipoprotein E (ApoE) allele frequencies were examined in a population-based sample (n = 475; age range 65-84 years; Amsterdam Study of the Elderly). The relation of ApoE ε4 with dementia and with various types of late-life depression was studied. Depression was measured with the Geriatric Mental State schedule. Dementia was diagnosed by DSM...
article 1998
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Jonker, C. (author), Schmand, B. (author), Lindeboom, J. (author), Havekes, L.M. (author), Launer, L.J. (author), Gaubius Instituut TNO (author)
Objective: To determine whether the apolipoprotein E ε4 allele (apoE ε4) is associated with cognitive decline in individuals with and without dementia, we conducted a 4-year longitudinal study of subjects with a range of cognitive function Setting: At baseline, respondents (n = 511) were randomly selected according to age and Mini-Mental State...
article 1998
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Boer, J.M.A. (author), Ehnholm, C. (author), Menzel, H.J. (author), Havekes, L.M. (author), Rosseneu, M. (author), O'Reilly, D.S.J. (author), Tiret, L. (author), Gaubius Instituut TNO (author)
To elucidate how the apolipoprotein (apo)E polymorphism and modifiable factors interact in explaining plasma lipid and apolipoprotein levels, we studied 1448 young adults (18 to 26 years old), participating in the European Atherosclerosis Research Study (EARS). Venous blood was collected after an overnight fast. Modifiable factors, eg, body mass...
article 1997
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Beekman, B. (author), Verzijl, N. (author), de Roos, J.A.D.M. (author), Koopman, J.L. (author), Tekoppele, J.M. (author), Leiden, Netherlands Gaubius Instituut TNO (author)
Doxycycline is known for its ability to inhibit matrix metalloproteinases (MMPs), a family of enzymes that play a role in cartilage breakdown in arthritides. Its prophylactic effect in reducing joint degradation in osteoarthritis is mainly attributed to this property. In this study, we show that doxycycline exhibits a profound inhibition of...
article 1997
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TNO Preventie en Gezondheid (author), van der Kerken, K. (author), de Raeve, H. (author), Goes, E. (author), van Meirvenne, S. (author), Radl, J. (author), van Riet, I. (author), Thielemans, K. (author), van Camp, B. (author)
The aim of this study was to evaluate the tissue infiltration and phenotypic adhesion profile of 5T2 multiple myeloma (MM) and 5T33 MM cells and to correlate it with that observed in human disease. For each line, 30 mice were intravenously inoculated with myeloma cells and at a clear-cut demonstrable serum paraprotein concentration; mice were...
article 1997
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Hoffer, M.J.V. (author), Niththyananthan, S. (author), Naoumova, R.P. (author), Kibirige, M.S. (author), Frants, R.R. (author), Havekes, L.M. (author), Thompson, G.R. (author), Gaubius Instituut TNO (author)
Apolipoprotein E (apoE) is one of the major protein constituents of chylomicron and very low density lipoprotein (VLDL) remnants and plays a central role as a ligand in the receptor-mediated uptake of these particles by the liver. Here we describe a new variant of apoE, apoE1-Hammersmith, which is associated with dominantly expressed type III...
article 1996
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de Jong, E.C. (author), Spanhaak, S. (author), Martens, B.P.M. (author), Kapsenberg, M.L. (author), Penninks, A.H. (author), Wierenga, E.A. (author), TNO Voeding (author)
Background: Increasing evidence indicates a prominent role of allergen-specific TH2 cells, with high IL-4 and IL-5 production and low interferon-γ production, in the regulation of IgE and eosinophil production in allergic disorders. However, most studies have concentrated on T cells reactive with inhalation allergens, whereas little is known...
article 1996
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Boomsma, D.I. (author), Kempen, H.J.M. (author), Gevers Leuven, J.A. (author), Havekes, L. (author), de Knijff, P. (author), Frants, R.R. (author), TNO Preventie en Gezondheid (author)
In a sample of Dutch families consisting of parents aged 35-65 years and their twin offspring aged 14-21 years, a significant difference between generations was observed in phenotypic variances and in genetic heritabilities for plasma levels of total cholesterol, triglycerides, high density lipoprotein (HDL) and low density lipoprotein (LDL)...
article 1996
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Hennis, B.C. (author), Boomsma, D.I. (author), van Boheemen, P.A. (author), Engesser, L. (author), Kievit, P. (author), Dooijewaard, G. (author), Kluft, C. (author), TNO Preventie en Gezondheid (author)
A pedigree-based maximum likelihood method developed by Lange et al. (12) was used to study the contribution of a newly defined di-allelic polymorphism in histidine-rich glycoprotein (HRG) to the plasma levels of HRG. In four families (n = 99) and 20 volunteers we found a heritability of 70%, an age effect of 3% and an effect of individual...
article 1995
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Hennis, B.C. (author), van Boheemen, P.A. (author), Wakabayashi, S. (author), Koide, T. (author), Hoffmann, J.J.M.L. (author), Kievit, P. (author), Dooijewaard, G. (author), Jansen, J.G. (author), Klutf, C. (author), TNO Preventie en Gezondheid (author)
Two forms of histidine-rich glycoprotein (HRG) were detected on SDS-PAGE by silver staining and immunoblotting after isolation of the protein from pooled plasma using immune-affinity chromatography followed by chromatography with heparin-Sepharose. Both forms were single-chain molecules and the apparent molecular weights of form 1 and form 2...
article 1995
Searched for: subject%3A%22Phenotype%22
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