Searched for: subject%3A%22Nucleic%255C%2BAcid%255C%2BHybridization%22
(1 - 8 of 8)
document
TNO Kwaliteit van Leven (author), Paauw, A. (author), Caspers, M.P.M. (author), Schuren, F.H.J. (author), Leverstein-van Hall, M.A. (author), Del├ętoile, A. (author), Montijn, R.C. (author), Verhoef, J. (author), Fluit, A.C. (author)
Background: Isolates of the Enterobacter cloacae complex have been increasingly isolated as nosocomial pathogens, but phenotypic identification of the E. cloacae complex is unreliable and irreproducible. Identification of species based on currently available genotyping tools is already superior to phenotypic identification, but the taxonomy of...
article 2008
document
Kienhuis, A.S. (author), Wortelboer, H.M. (author), Maas, W.J. (author), van Herwijnen, M. (author), Kleinjans, J.C.S. (author), van Delft, J.H.M. (author), Stierum, R.H. (author), TNO Kwaliteit van Leven (author)
A rapid decline of cytochrome P450 (CYP450) enzyme activities remains a drawback of rat hepatocyte-based in vitro cultures. Consequently, judgment of the toxic potential of compounds that need bioactivation by CYP450s may not be adequate using this model. In the present study, an improved hepatocyte-based in vitro system was developed with...
article 2007
document
te Biesebeke, R. (author), Levin, A. (author), Sagt, C. (author), Bartels, J. (author), Goosen, T. (author), Ram, A. (author), van den Hondel, C. (author), Punt, P. (author), TNO Kwaliteit van Leven (author)
Aspergillus oryzae requires polarized growth for colonization of solid substrates, and this growth phenotype differs from that seen in liquid medium. Various experimental approaches were used to identify genes that are differentially expressed when A. oryzae is grown on wheat kernels and in a wheat-based liquid medium. Hybridization of A. oryzae...
article 2005
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Punt, P.J. (author), Seiboth, B. (author), Weenink, X.O. (author), van Zeijl, C. (author), Lenders, M. (author), Konetschny, C. (author), Ram, A.F.J. (author), Montijn, R. (author), Kubicek, C.P. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
DNA fragments containing genetic information for five secretion-related small GTPases of Aspergillus niger (srgA-E) were isolated and identified as members of different Rab/Ypt subfamilies. This isolation and the search for similar sequences in fungal genomic and EST databases showed that, in contrast to Saccharomyces cerevisiae, filamentous...
article 2001
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van der Vossen, J.M.B.M. (author), Hofstra, H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The rapid identification of spoilage microorganisms is of eminent importance to the food industry. It provides the food industry with the opportunity to reduce economical losses by designing adequate intervention measures. The use of identification systems based on biochemical and physiological characteristics resulted often in disappointing...
article 1996
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Kaptein, A. (author), Roodenburg, L. (author), Princen, H.M.G. (author), Gaubius Institute TNO Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
Addition of sodium butyrate to the culture medium of the human hepatoma cell line Hep G2 resulted in a time- and dose-dependent increase in the secretion of apolipoprotein A-I (apo A-I) and apolipoprotein B100 (apo B100). After a 24 h preincubation period, a 2.4- and 2.2-fold increase in the secretion of apo A-I and apo B100 respectively was...
article 1991
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Horbach, G.J.M.J. (author), Princen, H.M.G. (author), van der Kroef, M. (author), van Bezooijen, C.F.A. (author), Yap, S.H. (author)
To investigate the regulation of age-related changes in albumin synthesis in the rat liver, total postnuclear RNA and polyribosomes, both membrane-bound and free, were prepared from livers of rats of different ages. By the use of a specific complementary DNA probe, the albumin mRNA sequence content was quantitated in these RNA fractions. These...
article 1984
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Pouwels, P.H. (author), Pannekoek, H. (author), Medisch Biologisch Laboratorium TNO Chemisch Laboratorium TNO (author)
Chemicals/CAS: tryptophan, 6912-86-3, 73-22-3; RNA, Bacterial; RNA, Viral; Tryptophan, 73-22-3; Tryptophan-tRNA Ligase, EC 6.1.1.2
article 1976
Searched for: subject%3A%22Nucleic%255C%2BAcid%255C%2BHybridization%22
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