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Caspers, M.P.M. (author), Lok, F. (author), Sinjorgo, K.M.C. (author), van Zeijl, M. (author), Nielsen, K.A. (author), Cameron-Mills, V. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)We have identified the major endo-β-l,4-xylanase (XYN-1) in the aleurone of germinating barley grain, and show that it is expressed as a precursor of Mr 61 500 with both N- and C-terminal propeptides. XYN-1 is synthesized as an inactive enzyme in the cytoplasm, and only becomes active at a late stage of germination when the aleurone ceases to...article 2001
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Sandbæk, G. (author), Bjørnsen, S. (author), Sobel, J.H. (author), Nieuwenhuizen, W. (author), Matsueda, G. (author), Brosstad, F. (author), Gaubius Instituut TNO (author)The aim of this study was to characterize soluble fibrin(ogen) species in human, arterial, in-vivo-formed thrombi, using the immunoblotting technique. Specimens were collected via intra-arterial catheters in six patients scheduled for catheter-directed thrombolysis. Unreduced and reduced samples of the supernatants from the arterial thrombi...article 2000
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Collen, A. (author), Smorenburg, S.M. (author), Peters, E. (author), Lupu, F. (author), Koolwijk, P. (author), van Noorden, C. (author), van Hinsbergh, V.W.M. (author), Gaubius instituut TNO (author)Cancer patients treated for venous thromboembolism with low molecular weight heparin (LMWH) have a better survival rate than patients treated with unfractionated heparin (UFH). Because fibrin-associated angiogenesis is an important determinant in the progression and metastasis of many solid tumors, the effects of heparins on in vitro...article 2000
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van der Werf, M.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A Baeyer-Villiger mono-oxygenase (BVMO), catalysing the NADPH- and oxygen-dependent oxidation of the monocyclic monoterpene ketones 1-hydroxy-2-oxolimonene, dihydrocarvone and menthone, was purified to homogeneity from Rhodococcus erythropolis DCL14. Monocyclic monoterpene ketone mono-oxygenase (MMKMO) is a monomeric enzyme of molecular mass 60...article 2000
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Holtman, W.L. (author), Roberts, M.R. (author), Wang, M. (author)Recently, we have demonstrated by two different methods that lipoxgenases (LOXs) and 14-3-3 proteins form interactions in barley embryos [Holtman, Roberts, Oppedijk, Testerink, van Zeijl and Wang (2000) FEBS Lett. 474, 48-52]. It was shown by both co-immunoprecipitations and surface-plasmon resonance experiments that 13-LOX, but not 9-LOX, forms...article 2000
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Beekman, B. (author), Drijfhout, J.W. (author), Ronday, H.K. (author), TeKoppele, J.M. (author), Gaubius instituut TNO (author)Elevated MMP activities are implicated in tissue degradation in, e.g., arthritis and cancer. The present study was designed to measure MMP enzyme activity in plasma. Free active MMP is unlikely to be present in plasma: upon entering the circulation, active MMP is expected to be captured by the proteinase inhibitor α2-macroglobulin (α2M)....article 1999
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Koppelman, S. (author), Knulst, A.C. (author), Koers, W.J. (author), Penninks, A.H. (author), Peppelman, H. (author), Vlooswijk, R. (author), Pigmans, I. (author), van Duijn, G. (author), Hessing, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Hazelnuts are widely used in the food industry owing to their nutritive value and taste. The amount of hazelnut present in a recipe is usually considered as a mark of quality. On the other hand, contamination of foods that normally do not contain hazelnuts is a threat for patients with a hazelnut allergy. For this reason, the availability of a...article 1999
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Thole, J.E.R. (author), Janson, A.A.M. (author), Cornelisse, Y. (author), Schreuder, G.M.T. (author), Wieles, B. (author), Naafs, B. (author), de Vries, R.R.P. (author), Ottenhoff, T.H.M. (author), Gaubius instituut TNO (author)The recognition of 16 mycobacterial Ags by a panel of T cell lines from leprosy patients and healthy exposed individuals from an endemic population was examined within the context of expressed HLA-DR molecules. Although overall no significant differences were found between the frequencies of Ag recognition in the different subject groups, when...article 1999
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van Baar, B.L.M. (author), Hulst, A.G. (author), Wils, E.R.J. (author), TNO Prins Maurits Laboratory, P.O. Box 45, 2280 AA, Rijswijk, Netherlands (author)Cholera toxin, one of the toxins that may be generated by various strains of the bacterium Vibrio cholerae, can be considered as a substance possibly used in biological warfare. The possibilities of characterising the toxin by liquid chromatography electrospray mass spectrometry (LC-ES-MS) were investigated. The toxin can be detected by flow...article 1999
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Aktypis, A. (author), Kalantzopoulos, G. (author), Huis in't Veld, J.H.J. (author), ten Brink, B. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)ACA-DC 0040 produced an antimicrobial agent, which was named thermophilin T, active against several lactic acid bacteria strains of different species and food spoilage bacteria, such as Clostridium sporogenes C22/10 and Cl. tyrobutyricum NCDO-1754. The crude antimicrobial compound is sensitive to proteolytic enzymes and α-amylase, heat-stable ...article 1998
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van Ruseler- Embden, J.G.H. (author), van Lieshout, L.M.C. (author), Binnema, D.J. (author), Hazenberg, M.P. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)1. An earlier study by our group revealed that the viscosity of faeces from patients with Crohn's disease is significantly lower than that of healthy subjects. This is due to low concentrations of a high-molecular-mass carbohydrate, probably of bacterial origin. The cause of this phenomenon might be the impaired barrier function of the gut...article 1998
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Gaubius instituut TNO (author), Hendriks, W.L. (author), van Vark, L.C. (author), Schoonderwoerd, K. (author), Jansen, H. (author), Havekes, L.M. (author)Lipoprotein lipase (LPL) purified from bovine milk showed variable abilities to stimulate the binding of low density lipoprotein (LDL) to J774 macrophages. The presence of a 37 kDa protein in the LPL sample seemed to be of importance for its stimulatory capacity. In order to investigate this, we isolated LPL from bovine milk via heparin...article 1998
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Myrup, B. (author), Jensen, T. (author), Gram, J. (author), Kluft, C. (author), Jespersen, J. (author), Deckert, T. (author), Gaubius Instituut TNO (author)OBJECTIVE - The high risk for cardiovascular disease in IDDM patients with nephropathy may be mediated by abnormal function of the vascular wall. We investigated whether heparin was able to modulate markers of vascular wall and hemostatic function in patients with incipient nephropathy. RESEARCH DESIGN AND METHODS - Thirty-five IDDM patients...article 1997
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Barak, R. (author), Ordentlich, A. (author), Barak, D. (author), Fischer, M. (author), Benschop, H.P. (author), de Jong, L.P.A. (author), Segall, Y. (author), Velan, B. (author), Shafferman, A. (author)While non-reactivability of cholinesterase from their phosphyl conjugates (aging) is attributed to an unimolecular process involving loss of alkyl group from the phosphyl moiety, no conclusive evidence is available that this is the only reaction path and involvement of other post-inhibitory processes cannot be ruled out, To address this issue,...article 1997
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Prins Maurits Laboratorium TNO (author), Bouma, R.H.B. (author), Nauta, W.J. (author), Arnauts, J.E.F. (author), van den Boomgaard, T. (author), Steuten, J.M. (author), Strathmann, H. (author)In the production of polyethylene foams by extrusion with alkanes as a blowing agent, significant changes in the dimensions of extruded products are encountered. The dimensional stability of a foam with a structure of closed cells is improved by blending the polymer with small amounts of a low molecular weight additive. It is believed that the...article 1997
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Nègre-Aminou, P. (author), Nemenoff, R.A. (author), Wood, M.R. (author), de la Houssaye, B.A. (author), Pfenninger, K.H. (author), Gaubius Instituut TNO (author)Nerve growth cones isolated from fetal rat brain exhibit in their cytosol a robust level of phospholipase A2 activity hydrolyzing phosphatidylinositol (PI) and phosphatidylethanolamine (PE) but not phosphatidylcholine (PC). Western blot analysis with an antibody to the well- characterized cytosolic phospholipase A2 (mol wt 85, 000) reveals only...article 1996
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Jespersen, S. (author), Ploemen, J.H.T.M. (author), Bladeren, P.J. (author), Niessen, W.M.A. (author), Tjaden, U.R. (author), van der Greef, J. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)A mass spectrometric method providing qualitative site-specific information regarding covalent modification of proteins is described. The method involves comparison of unmodified and modified proteins by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) peptide mapping in combination with site-specific mutagenesis of...article 1996
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Jespersen, S. (author), Koedam, J.A. (author), Hoogerbrugge, C.M. (author), Tjaden, U.R. (author), van der Greef, J. (author), van den Brande, J.L. (author), TNO Voeding (author)High molecular weight precursors of insulin-like growth factor II (IGF-II) were isolated from Cohn fraction IV of human plasma by ultrafiltration, affinity chromatography and reversed-phase high-performance liquid chromatography. Molecular weight determination by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of two...article 1996
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Gaubius Instituut TNO (author), Sakharov, D.V. (author), Lijnen, H.R. (author), Rijken, D.C. (author)Staphylokinase (STA), a protein of bacterial origin, induces highly fibrin-specific thrombolysis both in human plasma in vitro and in pilot clinical trials. Using fluorescence microscopy, we investigated the spatial distribution of fluorescein isothiocyanate (FITC)-labeled STA during lysis of a plasma clot and its binding to purified fibrin...article 1996
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Hennis, B.C. (author), Boomsma, D.I. (author), van Boheemen, P.A. (author), Engesser, L. (author), Kievit, P. (author), Dooijewaard, G. (author), Kluft, C. (author), TNO Preventie en Gezondheid (author)A pedigree-based maximum likelihood method developed by Lange et al. (12) was used to study the contribution of a newly defined di-allelic polymorphism in histidine-rich glycoprotein (HRG) to the plasma levels of HRG. In four families (n = 99) and 20 volunteers we found a heritability of 70%, an age effect of 3% and an effect of individual...article 1995