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Bosker, W.T.E. (author), Ágoston, K. (author), Cohen Stuart, M.A. (author), Norde, W. (author), Timmermans, J.W. (author), Slaghek, T.M. (author)
Linear block copolymers of polystyrene and polysaccharide were synthesized using a block synthesis method with amino-terminated polystyrene and sodium cyanoborohydride as reducing agent. Different types of polysaccharides, dextrans, and maltodextrins with various molecular weights were used. IR spectroscopy indicated a successful coupling....
article 2003
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Lokman, B.C. (author), Joosten, V. (author), Hovenkamp, J. (author), Gouka, R.J. (author), Verrips, C.T. (author), van den Hondel, C.A.M.J.J. (author)
The heterologous production of Arthromyces ramosus peroxidase (ARP) was analysed in the filamentous fungus Aspergillus awamori under control of the inducible endoxylanase promoter. Secretion of active ARP was achieved up to 800 mg l-1 in shake flask cultures. Western blot analysis showed that an rARP product of the correct molecular weight was...
article 2003
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Faassen, F. (author), Kelder, J. (author), Lenders, J. (author), Onderwater, R. (author), Vromans, H. (author)
Purpose. The purpose of this work was to study the relevant physicochemical properties for the absorption of steroids. Methods. Various physicochemical properties of steroids were calculated (molecular weight, ClogP, static polar surface area [PSA], etc.). Within this series of steroids, different pharmacological groups were defined. Based on...
article 2003
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PG (author), Tchetverikov, I. (author), Verzijl, N. (author), Huizinga, T.W.J. (author), TeKoppele, J.M. (author), Hanemaaijer, R. (author), de Groot, J. (author)
Objective. The aim of the present study was to analyze alpha2Macroglobulin/MMP (alpha2M/MMP) complex formation and to investigate whether MMP activity in alpha2M/MMP complexes in serum can be used as a disease marker in rheumatoid arthritis (RA). Methods. High and low molecular weight (H/LMW) substrates and inhibitors and size exclusion were...
article 2003
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Helmroth, E. (author), Rijk, R. (author), Dekker, M. (author), Jongen, W. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Migration of low-molecular weight compounds is one of the most important problems of packaging plastics and other plastics intended to come into contact with food products. Since migration experiments are time consuming and expensive, predictive modelling has been introduced as a promising alternative. The main objective of this article is to...
article 2002
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Piersma, S.R. (author), van de Pijpekamp, A. (author), Wijngaards, G. (author), Gruppen, H. (author), Boumans, H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A mass spectrometric approach was chosen to quantify and localise in vitro enzymatically modified glutamine (Gln) residues in a glutamine-rich protein. This protein (named dB1), a cloned domain of the high molecular weight wheat glutenin subunit Dx5, was modified by microbial transglutaminase (TGase) using hydroxylamine as the amine donor. Using...
article 2002
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van de Velde, F. (author), Rollema, H.S. (author), Grinberg, N.V. (author), Burova, T.V. (author), Grinberg, V.Ya. (author), Hans Tromp, R. (author), TNO Voeding (author)
A series of ι-carrageenans containing different amounts of v-carrageenan (0-23 monomer %) have been prepared from neutrally extracted carrageenan of Eucheuma denticulatum. v-Carrageenan is the biochemical precursor of ι-carrageenan. The conformational order-disorder transition and rheological properties of these carrageenans were studied using...
article 2002
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Record, E. (author), Punt, P.J. (author), Chamkha, M. (author), Labat, M. (author), van den Hondel, C.A.M.J.J. (author), Asther, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Pycnoporus cinnabarinus laccase lac1 gene was overexpressed in Aspergillus niger, a well-known fungal host producing a large amount of homologous or heterologous enzymes for industrial applications. The corresponding cDNA was placed under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter as a strong and constitutive promoter....
article 2002
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Smit, E. (author), Jager, D. (author), Martinez, B. (author), Tielen, F.J. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The structure of the crystallisation domain, SAN, of the S A-protein of Lactobacillus acidophilus ATCC 4356 was analysed by insertion and deletion mutagenesis, and by proteolytic treatment. Mutant S A-protein synthesised in Escherichia coli with 7-13 amino acid insertions near the N terminus or within regions of sequence variation in SAN (amino...
article 2002
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TNO Industrie en Techniek (author), Wouters, M.E.L. (author), Goossens, J.G.P. (author), Binsbergen, F.L. (author)
The thermoreversible nature of the ionic associations in aggregates makes ionomers with a low glass transition temperature interesting candidates for thermoplastic elastomers. In this paper, a low-Tg ionomer based on low molecular weight ethylene-propylene copolymers modified by maleic anhydride (MAn-g-EPM) is introduced, and the morphology of a...
article 2002
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Dybing, E. (author), Doe, J. (author), Groten, J. (author), Kleiner, J. (author), O'Brien, J. (author), Renwick, A.G. (author), Schlatter, J. (author), Steinberg, P. (author), Tritscher, A. (author), Walker, R. (author), Younes, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Hazard characterisation of low molecular weight chemicals in food and diet generally use a no-observed-adverse-effect level (NOAEL) or a benchmark dose as the starting point. For hazards that are considered not to have thresholds for their mode of action, low-dose extrapolation and other modelling approaches may be applied. The default position...
article 2002
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van Baar, B.L.M. (author), Hulst, A.G. (author), Roberts, B. (author), Wils, E.R.J. (author), Prins Maurits Laboratorium TNO (author)
A method was developed for the liquid chromatographic-mass spectrometric (LC-MS) identification of extremely neurotoxic toxins. The method combines sample treatment in a safety containment and analysis of detoxified material in a common laboratory facility. The method was applied to the characterization of neat tetanus toxin and subsequent...
article 2002
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Korthout, H.A.A.J (author), Caspers, M.P.M (author), Kottenhagen, M.J. (author), Helmer, Q. (author), Wang, M. (author)
Over the past few years the presence of p53-like proteins in plants was frequently reported, by using the monoclonal antibody Pab240. By means of protein purification and screening a cDNA library, a Pab240 cross-reacting protein and a cDNA clone were isolated from barley. Peptide- and DNA-sequence analysis identified one and the same protein: 2...
article 2002
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TNO Voeding (author), Fischer, M. (author), Gruppen, H. (author), Piersma, S.R. (author), Kofod, L.V. (author), Schols, H.A. (author), Voragen, A.G.J. (author)
With the purpose of analyzing the size and composition of enzyme-unextractable proteins in differently heat-treated soybean meals, a selection of extractants was screened for their ability to extract these proteins from enzyme-unextractable residues. The largest effects were obtained with urea, urea plus β-mercaptoethanol, and dilute alkali; the...
article 2002
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Meuleman, E.E.B. (author), Willemsen, J.H.A. (author), Mulder, M.H.V. (author), Strathmann, H. (author)
Various types of ethylene-propylene-diene terpolymers (EPDM) and crosslinking procedures have been investigated with pervaporation, vapor sorption, liquid sorption and gas permeation experiments. The EPDM parameters that have been changed are ethylene content, molecular weight, choice of third monomer, type of branching and various crosslinking...
article 2001
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Bos, M.A. (author), van Vliet, T. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Proteins and low molecular weight (LMW) surfactants are widely used for the physical stabilisation of many emulsions and foam based food products. The formation and stabilisation of these emulsions and foams depend strongly on the interfacial properties of the proteins and the LMW surfactants. Therefore these properties have been studied...
article 2001
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Collen, A. (author), Maas, A. (author), Kooistra, T. (author), Lupu, F. (author), Grimbergen, J. (author), Haas, F.J.L.M. (author), Biesma, D.H. (author), Koolwijk, P. (author), Koopman, J. (author), van Hinsbergh, V.W.M. (author), Gaubius instituut TNO (author)
A congenital dysfibrinogenemia, fibrinogenNieuwegein, was discovered in a young man without any thromboembolic complications or bleeding. A homozygous insertion of a single nucleotide (C) in codon Aα 453 (Pro) introduced a stop codon at position 454, which resulted in the deletion of the Carboxyl-terminal segment Aα 454-610. The ensuing unpaired...
article 2001
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Kros, A. (author), van Hövell, W.F.M. (author), Sommerdijk, N.A.J.M. (author), Nolte, R.J.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Amperometric biosensors for the recognition of glucose oxidase (GOx) based on poly(3,4-ethylenedioxythiophene) (PEDOT) were fabricated for the first time. The resulting biosensor has potential applications for long-term glucose measurements.
article 2001
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Hijum, S.A.F.T. (author), Bonting, K. (author), van der Maarel, M.J.E.V. (author), Dijkhuizen, L. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and...
article 2001
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Caspers, M.P.M. (author), Lok, F. (author), Sinjorgo, K.M.C. (author), van Zeijl, M. (author), Nielsen, K.A. (author), Cameron-Mills, V. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
We have identified the major endo-β-l,4-xylanase (XYN-1) in the aleurone of germinating barley grain, and show that it is expressed as a precursor of Mr 61 500 with both N- and C-terminal propeptides. XYN-1 is synthesized as an inactive enzyme in the cytoplasm, and only becomes active at a late stage of germination when the aleurone ceases to...
article 2001
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