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Janssen, P.J.M. (author), Bosland, M.C. (author), van Hees, J.P. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)The effects of feeding inorganic tin on the gastrointestinal tract were examined in rats. Three groups of male weanling Wistar rats were fed a diet to which 0, 250, or 500 ppm Sn2+ had been added as SnCl2. A fourth group was subjected to feed restriction by pair feeding with the 500-ppm group. Comparison of the data from the tin-fed groups with...article 1985
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Feron, V.J. (author), Immel, H.R. (author), Spit, B.J. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)The trachea of Syrian golden hamsters was injured by electrocoagulation. The lesions induced as well as the repair process were studied by light and scanning electron microscopy. The changes observed were considered to create the proper conditions for studying the significance of severe focal damage to the respiratory tract for the formation of...article 1984
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Beems, R.B. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)Chemicals/CAS: benzo[a]pyrene, 50-32-8; retinol, 68-26-8, 82445-97-4; Benzo(a)pyrene, 50-32-8; Benzopyrenes; Vitamin A, 11103-57-4article 1984
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Gaubius instituut TNO (author), Havekes, L. (author), van Hinsbergh, V. (author), Kempen, H.J. (author), Emeis, J. (author)The human hepatoma cell line Hep G2 was studied with respect to metabolism of human low-density lipoprotein (LDL). The Hep G2 cells bind, take up and degrade human LDL with a high-affinity saturable and with a low-affinity non-saturable component. The high-affinity binding possesses a K(D) of 25 nM-LDL and a maximal amount of binding of about 70...article 1983
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Willems, M.I. (author), de Schepper, G.G. (author), Wibowo, A.A.E. (author), Immel, H.R. (author), Dietrich, A.J.J. (author), Zielhuis, R.L. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The influence of lead on sperm morphology, sister chromatid exchanges or on micronuclei formation was studied on male rabbits after exposure to doses of 0, 0.25, and 0.50 mg lead acetate/kg body weight subcutaneously injected three times a week during 14 weeks, each on a group of five rabbits. At the end of exposure phase the lead in blood...article 1982
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Beems, R.B. (author), Spit, B.J. (author), Koeter, H.B.W.M. (author), Feron, V.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A variety of gastric lesions can be induced in rats by sodium metabisulfite, including the formation of scattered hyperplastic glands in the fundic mucosa which are exclusively lined by large uniform cells, laden with acidophilic granules. Light and electron microscopy, as enzyme histochemistry, showed that the cells lining these glands are...article 1982
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Emeis, J.J. (author), Vermeer, B.J. (author), Havekes, L. (author), Gaubius instituut TNO (author)We visualized the apolipoproteins of high density lipoproteins (HDL) that were bound to cultured human fibroblasts at the ultrastructural level, with an immunoenzymecytochemical method. By using antisera against (a) apolipoprotein AI (a major apolipoprotein of HDL) and (b) apolipoprotein E (an apolipoprotein present in HDL), the binding of HDL...article 1981
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Spit, B.J. (author), Feron, V.J. (author), Hendriksen, C.F.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The hepatic angiosarcoma studied was from a male Wistar rat having been exposed to vinyl chloride monomer (VCM) by the oral route for 120 weeks. Widened sinusoids lined by large electron-lucent, spindle-shaped tumor cells and membrane-bound nuclear-free structures were seen in the transitional zone between the tumor mass and the adjacent liver...article 1981
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Gaubius Instituut TNO (author), Havekes, L. (author), Vermeer, B.J. (author), Brugman, T. (author), Emeis, J. (author)Chemicals/CAS: Lipoprotein(a); Lipoproteins; Lipoproteins, LDL; Receptors, Cell Surface; Receptors, LDLarticle 1981
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Centraal Instituut voor Voedingsonderzoek TNO (author), Spit, B.J. (author), Wibowo, A.A.E. (author), Ferron, V.J. (author), Zielhuis, R.L. (author)Electron microscopical studies were carried out on the kidneys of rabbits given s.c. injections of 0 (control), 0.25 or 0.50 mg lead acetate/kg b.w. 3 times a week during 14 weeks. At the end of the experimental period the animals had lead blood levels of 60, 500 and 600 ??g/1 whole blood respectively. Treatment-related renal changes were found...article 1981
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Meihuizen, S.P. (author), Blansjaar, N. (author), Instituut voor experimentele gerontologie TNO (author)Hepatocyte morphology of the three zones of the liver lobule was evaluated by stereological analysis in 3-month-old and 35-month-old virgin female WAG/Rij rats. In the old rats, the volume of the nucleus and cytoplasm, and volume densities of smooth endoplasmic reticulum and lysosomes were increased in all areas of the liver lobule, while the...article 1980
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Schuit, H.R.E. (author), Hijmans, W. (author), Instituut voor experimentele gerontologie TNO (author)The presence of surface-associated immunoglobulins and Fc receptors on mononuclear cells from normal human blood waas investigated by the direct immunofluorescence technique combined with phase-contrast microscopy. Formaldehyde-fixed cells were compared to unfixed cells and to cells preincubated at 37??C. In the unfixed samples a separate...article 1980
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Feron, V.J. (author), Kroes, R. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Wistar rats were exposed to atmospheres containing 0 (control) or 5000 ppm vinyl chloride monomer (VCM), 7 h/day, 5 days/week, for a period of 52 weeks. After 4, 13, 26 and 52 weeks each time 10 rats/sex/group were killed and subjected to extensive examinations. The present paper describes the morphological changes observed in the respiratory...article 1979
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van Bekkum, D.W. (author), van den Engh, G.J. (author), Wagemaker, G. (author), Radiobiologisch Instituut TNO (author)A review is presented of the experiments that resulted in the identification of a specific morphologic entity representing the pluripotential hemopoietic stem cell (HSC) in mouse bone marrow. This entity was subsequently discovered in concentrated HSC preparations from bone marrow of rats, monkeys, and humans. In the mouse, a set of physical...article 1979
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Knook, D.L. (author), Instituut voor experimentele gerontologie TNO (author)Ageing is associated with declines in the functional capacities of several organs. General causes for the decline can be divided into: 1. intrinsic cellular causes and 2. extracellular causes, e.g., changes in blood circulation and distribution. For the first group of causes, there is evidence for a decrease in the numbers of long-lived cells in...article 1978
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Feron, V.J. (author), Kruysse, A. (author), Til, H.P. (author), Immel, H.R. (author), Centraal instituut voor voedingsonderzoek TNO (author)The subacute inhalation toxicity of acrolein was examined in 4 groups of 20 hamsters, 12 rats and 4 rabbits each, exposed repeatedly to acrolein vapour at concentrations of 0, 0.4, 1.4 and 4.9 ppm (6 hr/day, 5 days/week) for a 13-week period. The most important effects found at the highest level included mortality in rats, ocular and nasal...article 1978
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Kruysse, A. (author), Feron, V.J. (author), Immel, H.R. (author), Centraal instituut voor voedingsonderzoek TNO (author)The inhalation toxicity of peroxyacetyl nitrate (PAN) was examined in acute (single exposure), subacute (4-wk repeated exposure) and subchronic (13-wk repeated exposure) studies in rats. The 4-hr LC50 was found to be 95 ppm. In the 4-wk study rats were exposed to 0, 0.9, 4.1 or 11.8 ppm PAN vapor for 6 hr/day, 5 days/wk. Exposure to 11.8 ppm...article 1977
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- Mattern, I.E. (author), Medisch Biologisch Laboratorium RVO-TNO (author) article 1976
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van Beek, L. (author), Feron, V.J. (author), de Groot, A.P. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Alkali treated spun soy isolate was fed to rats at dietary levels of 5, 10, and 20% for a period of 90 days. The results failed to reveal any adverse effects on general appearance, growth, feeding efficiency, hematological indices, blood serum values, urine composition, kidney function, organ weights, and gross and microscopic pathology except...article 1974
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Instituut voor experimentele gerontologie TNO (author), Haaijman, J.J. (author), van Dalen, J.P.R. (author)In order to obtain microfluorometric standards for fluorescein isothiocyanate (FITC) and tetramethyl rhodamine isothiocyanate (TRITC), Sephadex G 25 Superfine beads (diameter 10-40 μ) were aminated with aminoethylsulphuric acid and stained with FITC or TRITC. Several parameters of the reaction were assessed. A high degree of linearity was...article 1974