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Willems, M.I. (author), de Schepper, G.G. (author), Wibowo, A.A.E. (author), Immel, H.R. (author), Dietrich, A.J.J. (author), Zielhuis, R.L. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The influence of lead on sperm morphology, sister chromatid exchanges or on micronuclei formation was studied on male rabbits after exposure to doses of 0, 0.25, and 0.50 mg lead acetate/kg body weight subcutaneously injected three times a week during 14 weeks, each on a group of five rabbits. At the end of exposure phase the lead in blood...article 1982
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Beems, R.B. (author), Spit, B.J. (author), Koeter, H.B.W.M. (author), Feron, V.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A variety of gastric lesions can be induced in rats by sodium metabisulfite, including the formation of scattered hyperplastic glands in the fundic mucosa which are exclusively lined by large uniform cells, laden with acidophilic granules. Light and electron microscopy, as enzyme histochemistry, showed that the cells lining these glands are...article 1982
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Emeis, J.J. (author), Vermeer, B.J. (author), Havekes, L. (author), Gaubius instituut TNO (author)We visualized the apolipoproteins of high density lipoproteins (HDL) that were bound to cultured human fibroblasts at the ultrastructural level, with an immunoenzymecytochemical method. By using antisera against (a) apolipoprotein AI (a major apolipoprotein of HDL) and (b) apolipoprotein E (an apolipoprotein present in HDL), the binding of HDL...article 1981
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Spit, B.J. (author), Feron, V.J. (author), Hendriksen, C.F.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The hepatic angiosarcoma studied was from a male Wistar rat having been exposed to vinyl chloride monomer (VCM) by the oral route for 120 weeks. Widened sinusoids lined by large electron-lucent, spindle-shaped tumor cells and membrane-bound nuclear-free structures were seen in the transitional zone between the tumor mass and the adjacent liver...article 1981
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Gaubius Instituut TNO (author), Havekes, L. (author), Vermeer, B.J. (author), Brugman, T. (author), Emeis, J. (author)Chemicals/CAS: Lipoprotein(a); Lipoproteins; Lipoproteins, LDL; Receptors, Cell Surface; Receptors, LDLarticle 1981
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Centraal Instituut voor Voedingsonderzoek TNO (author), Spit, B.J. (author), Wibowo, A.A.E. (author), Ferron, V.J. (author), Zielhuis, R.L. (author)Electron microscopical studies were carried out on the kidneys of rabbits given s.c. injections of 0 (control), 0.25 or 0.50 mg lead acetate/kg b.w. 3 times a week during 14 weeks. At the end of the experimental period the animals had lead blood levels of 60, 500 and 600 ??g/1 whole blood respectively. Treatment-related renal changes were found...article 1981
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Meihuizen, S.P. (author), Blansjaar, N. (author), Instituut voor experimentele gerontologie TNO (author)Hepatocyte morphology of the three zones of the liver lobule was evaluated by stereological analysis in 3-month-old and 35-month-old virgin female WAG/Rij rats. In the old rats, the volume of the nucleus and cytoplasm, and volume densities of smooth endoplasmic reticulum and lysosomes were increased in all areas of the liver lobule, while the...article 1980
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Schuit, H.R.E. (author), Hijmans, W. (author), Instituut voor experimentele gerontologie TNO (author)The presence of surface-associated immunoglobulins and Fc receptors on mononuclear cells from normal human blood waas investigated by the direct immunofluorescence technique combined with phase-contrast microscopy. Formaldehyde-fixed cells were compared to unfixed cells and to cells preincubated at 37??C. In the unfixed samples a separate...article 1980
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Feron, V.J. (author), Kroes, R. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Wistar rats were exposed to atmospheres containing 0 (control) or 5000 ppm vinyl chloride monomer (VCM), 7 h/day, 5 days/week, for a period of 52 weeks. After 4, 13, 26 and 52 weeks each time 10 rats/sex/group were killed and subjected to extensive examinations. The present paper describes the morphological changes observed in the respiratory...article 1979
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van Bekkum, D.W. (author), van den Engh, G.J. (author), Wagemaker, G. (author), Radiobiologisch Instituut TNO (author)A review is presented of the experiments that resulted in the identification of a specific morphologic entity representing the pluripotential hemopoietic stem cell (HSC) in mouse bone marrow. This entity was subsequently discovered in concentrated HSC preparations from bone marrow of rats, monkeys, and humans. In the mouse, a set of physical...article 1979
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Knook, D.L. (author), Instituut voor experimentele gerontologie TNO (author)Ageing is associated with declines in the functional capacities of several organs. General causes for the decline can be divided into: 1. intrinsic cellular causes and 2. extracellular causes, e.g., changes in blood circulation and distribution. For the first group of causes, there is evidence for a decrease in the numbers of long-lived cells in...article 1978
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Feron, V.J. (author), Kruysse, A. (author), Til, H.P. (author), Immel, H.R. (author), Centraal instituut voor voedingsonderzoek TNO (author)The subacute inhalation toxicity of acrolein was examined in 4 groups of 20 hamsters, 12 rats and 4 rabbits each, exposed repeatedly to acrolein vapour at concentrations of 0, 0.4, 1.4 and 4.9 ppm (6 hr/day, 5 days/week) for a 13-week period. The most important effects found at the highest level included mortality in rats, ocular and nasal...article 1978
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Kruysse, A. (author), Feron, V.J. (author), Immel, H.R. (author), Centraal instituut voor voedingsonderzoek TNO (author)The inhalation toxicity of peroxyacetyl nitrate (PAN) was examined in acute (single exposure), subacute (4-wk repeated exposure) and subchronic (13-wk repeated exposure) studies in rats. The 4-hr LC50 was found to be 95 ppm. In the 4-wk study rats were exposed to 0, 0.9, 4.1 or 11.8 ppm PAN vapor for 6 hr/day, 5 days/wk. Exposure to 11.8 ppm...article 1977
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- Mattern, I.E. (author), Medisch Biologisch Laboratorium RVO-TNO (author) article 1976
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van Beek, L. (author), Feron, V.J. (author), de Groot, A.P. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Alkali treated spun soy isolate was fed to rats at dietary levels of 5, 10, and 20% for a period of 90 days. The results failed to reveal any adverse effects on general appearance, growth, feeding efficiency, hematological indices, blood serum values, urine composition, kidney function, organ weights, and gross and microscopic pathology except...article 1974
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Instituut voor experimentele gerontologie TNO (author), Haaijman, J.J. (author), van Dalen, J.P.R. (author)In order to obtain microfluorometric standards for fluorescein isothiocyanate (FITC) and tetramethyl rhodamine isothiocyanate (TRITC), Sephadex G 25 Superfine beads (diameter 10-40 μ) were aminated with aminoethylsulphuric acid and stained with FITC or TRITC. Several parameters of the reaction were assessed. A high degree of linearity was...article 1974
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Molenaar, P.C. (author), Polak, R.L. (author), Nickolson, V.J. (author), Medisch Biologisch Laboratorium TNO (author)Slices from rat brain cortex were incubated for either 5 or 60 min in a medium containing [3H]choline and 4.7 or 25 mM KCl. Bioassayable ACh and labelled ACh were determined in the incubation medium, in the total tissue homogenate and in subcellular fractions. Raising the KCl concentration from 4.7 to 25 mM stimulated the release and synthesis...article 1973
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Zeedijk, H.B. (author), Metaalinstituut TNO (author)Asbestos bodies and asbestos fibers were found in the lungs of a mesothelioma patient and analysed by electron microscopy. The bodies were normal asbestos bodies with amphibole-asbestos fibers as kernels. The free asbestos fibers, present in the lungs, mainly were chrysotile-asbestos. © 1973 Springer-Verlag. Chemicals/CAS: asbestos, 1332-21-4;...article 1973
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Instituut voor experimentele gerontologie TNO (author), Dicke, K.A. (author), van Noord, M.J. (author), Maat, B. (author)The colony forming unit culture (CFU C) in the thin layer agar colony technique is considered to be representative for hemopoietic stem cells (HSC), according to studies in mouse and monkey bone marrow. Using this in vitro assay as a guide, stem cell concentrates were prepared from monkey and human bone marrow by repeated density gradient...article 1973
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- Offers, S. (author), Bentvelzen, P. (author), Radiobiologisch Instituut TNO (author) article 1971