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TNO Preventie en Gezondheid Gaubius Instituut TNO (author), Sakharov, D.V. (author), Nagelkerkel, J.F. (author), Rijken, D.C. (author)Binding of components of the fibrinolytic system to fibrin is important for the regulation of fibrinolysis. In this study, decomposition of the fibrin network and binding of plasminogen and plasminogen activators (PAs) to fibrin during lysis of a plasma clot were investigated with confocal microscopy using fluorescein-labeled preparations of...article 1996
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Claassen, I.J.T.M. (author), Osterhaus, A.D.M.E. (author), Claassen, E. (author), Gaubius Instituut TNO (author)Several mechanisms have been postulated to explain the relatively high immunogenicity of antigens presented in immune-stimulating complexes (iscom). Their potency can in part be explained by the specific targeting of these structures to cells presenting antigens to the immune system. However, until now no method for the subcellular detection of...article 1995
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- Somogy, P. (author), van Bree, J.L.M.J. (author), Prins Maurits Laboratorium TNO (author) conference paper 1995
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Punt, P.J. (author), Veldhuisen, G. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Although the application of filamentous fungi, such as Aspergillus niger for the production of extracellular proteins is well established for several decades, hardly any information is available about the molecular mechanisms of the process of protein secretion in these organisms. Two lines of research initiated towards a systematic analysis of...article 1994
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Vink, A.A. (author), Sontag, Y. (author), de Gruijl, F.R. (author), Roza, L. (author), Baan, R.A. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)An immunocytochemical method was developed to study in vivo induction and removal of DNA damage in a specific cell population in the epidermis of hairless mice after ultraviolet B (UVB) exposure: the immunocompetent antigen-presenting Langerhans cells. To this aim, Ia+ cells, which are representative for epidermal Langerhans cells, were compared...article 1994
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Bosma, A. (author), Seifert, W.F. (author), de Thiel van - Ruiter, G.C.F. (author), van Leeuwen, R.E.W. (author), Blauw, B. (author), Roholl, P. (author), Knook, D.L. (author), Brouwer, A. (author), Gaubius Laboratory TNO Preventie en Gezondheid Instituut voor verouderings- en vaatziekten onderzoek TNO (author)Rats were malnourished for 12 months with a highly inadequate fat-rich, calorie-sufficient but otherwise poly-deficient liquid diet composed of mashed potatoes with mayonnaise, comparable with the nutritional intake of many chronic alcoholics. When alcohol was incorporated into this diet, administered as whisky in drinking water available ad...article 1994
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TNO Preventie en Gezondheid (author), Roholl, P.J.M. (author), Blauw, E. (author), Zurcher, C. (author), Dormans, J.A.M.A. (author), Theuns, H.M. (author)Bone is subject to continuous remodeling throughout life. The age-related loss of (trabecular) bone, leading to senile osteopenia, is mainly due to impaired bone formation. Osteoblasts (OB) and osteoclasts (OC) have been identified as playing a crucial role in the process of bone turnover, but the contribution made by their precursors is not...article 1994
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Koopman, J. (author), Haverkate, F. (author), Grimbergen, J. (author), Lord, S.T. (author), Mosesson, M.W. (author), DiOrio, J.P. (author), Siebenlist, K.S. (author), Legrand, C. (author), Soria, J. (author), Soria, C. (author), Caen, J.P. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)The molecular defect in the abnormal fibrinogen Dusart (Paris V) that is associated with thrombophilia was determined by sequence analysis of genomic DNA that had been amplified using the polymerase chain reaction. The propositus was heterozygous for a single base change (C → T) in the Aα- chain gene, resulting in the amino acid substitution Aα...article 1993
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Spit, B.J. (author), Bretschneider, F. (author), Hendriksen, E.G.J. (author), Kuper, C.F. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The distribution of nerve fibres in the mucosa of the nasal septum of the rat was investigated by means of transmission electron microscopy on transverse and tangential ultrathin sections. Near the basement membrane of respiratory and squamous epithelium, a rather dense network of unmyelinated nerve fibres occurs. Some fibres in the respiratory...article 1993
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Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author), Esbach, S. (author), Pieters, M.N. (author), van der Boom, J. (author), Schouten, D. (author), van der Heyde, M.N. (author), Roholl, P.J.M. (author), Brouwer, A. (author), van Berkel, T.J.C. (author), Knook, D.L. (author)The interaction of oxidized human low-density lipoproteins with human and rat liver was analyzed by light and electron microscopy. At the light microscopic level oxidized low-density lipoprotein was visualized by the fluorescent dye 1,1' dioctadecyl 3,3,3',3' tetramethyl indocarbocyanine perchlorate, whereas at the electron microscopic level, an...article 1993
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Gezondheidsorganisatie TNO Instituut voor verouderings- en vaatziekten onderzoek TNO (author), HogenEsch, H. (author), Niewold, T.A. (author), Higuchi, K. (author), Tooten, P.C.J. (author), Gruys, E. (author), Radl, J. (author)The light microscopic and immunohistochemical features of a novel localized senile amyloidosis in the gastrointestinal tract of C57BL/Ka mice are described. Senile gastrointestinal amyloidosis was predominantly found in the lamina propria of the ileum, cecum and stomach and infrequently in other segments of the gastrointestinal tract. The Congo...article 1993
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Claassen, E. (author), Medisch Biologisch Laboratorium TNO (author)A fast and simple method for the in vivo/in situ detection of liposomes is described. Utilizing lipophilic carbocyanine dyes, DiI and DiO, yellow (or red) and green fluorescent liposomes can be visualised with routinely available filters. The main advantages of the method are (i) the vesicles can be labelled after they are formed and (ii) the...article 1992
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Bosma, A. (author), Seifert, W.F. (author), Wilson, J.H.P. (author), Roholl, P.J.M. (author), Brouwer, A. (author), Knook, D.L. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)Rats of two strains (BN/BiRij and WAG/Rij) were fed the ethanol-containing Lieber-De Carli liquid diet supplemented with high amounts of vitamin A for 16 months. In contrast to Lieber and co-workers, who showed liver fibrosis developing within 9 months on the same diet in Sprague-Dawley rats, we were unable to demonstrate a histological and...article 1991
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Claassen, E. (author), Medisch Biologisch Laboratorium TNO (author)Letter to the editorsarticle 1991
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Rutten, A.A.J.J.L. (author), Bequet-Passelecq, B.G.A.G.G. (author), Koeter, H.B.W.M. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)A new method was developed for rabbit skin organ culture. In a two-compartment model, skin discs were cultured on a Millicell-HA insert unit with a microporous membrane which allows transport of culture medium via the dermis into the epidermis, whereas the epidermal side remains free of direct contact with culture medium. In this relatively...article 1990
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Bosland, M.C. (author), Prinsen, M.K. (author), Dirksen, T.J.M. (author), Spit, B.J. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)Carcinomas of the rat prostate induced by a single injection of N-methyl-N-nitrosourea, 7,12-dimethylbenz(a)anthracene, and 3,2'-dimethyl-4-aminobiphenyl, after sequential treatment with cyproterone acetate and testosterone propionate, were evaluated as potential animal model for prostatic cancer. All ten carcinomas examined were located in the...article 1990
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Gaubius Instituut TNO (author), Wasser, M.N.J.M. (author), Pauwels, E.K.J. (author), Nieuwenhuizen, W. (author)This paper describes the production and characterization of an antifibrin monoclonal antibody, produced by immunizing mice with fibrinogen degradation fragment by the antibody (designated Y22) is directed against a conformation dependent epitope in the D-domain of fibrin Y22 was labeled with the radionuclide Tc-99m. Experiments in vitro and in...article 1990
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Zwart, A. (author), Woutersen, R.A. (author), Wilmer, J.W.G.M. (author), Spit, B.J. (author), Feron, V.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)To study in detail possible effects of low concentrations of formaldehyde on the nasal epithelium, Wistar rats were exposed to 0, 0.3, 1 and 3 ppm formaldehyde vapour for 6 h/day, 5 days/week during 3 days or 13 weeks, using in vivo [3H]thymidine labeling for cell proliferation studies, and light and electron microscopy for detecting...article 1988
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Instituut voor Experimentele Gerontologie TNO (author), Hendriks, H.F.J. (author), Brekelmans, P.J.A.M. (author), Buytenhek, R. (author), Brouwer, A. (author), Margreet de Leeuw, A. (author), Knook, D.L. (author)The neutral lipid and phospholipid compositions of purified sinusoidal (fat-storing, endothelial and Kupffer) cells, parenchymal cells and liver homogenates were determined by thin layer chromatography. In addition, the retinoid content of the same purified cell populations was determined by high performance liquid chromatography. From each cell...article 1987
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van Graft, M. (author), Spit, B.J. (author), Immel, H.R. (author), Feron, V.J. (author), Instituut CIVO-Toxicologie en voeding TNO (author)In a one-year serial sacrifice study 10 mg glass fibres (length, 95% ?? 20 ??m, 89% ?? 12 ??m, 58% ?? 5 ??m and 25% ?? 2 ??m; diameter, 88% ?? 1.0 ??m, 60% ?? 0.5 ??m and 31% ??0.25 ??m) suspended in 0.2 ml saline solution were administered to Syrian golden hamsters by a single intratracheal instillation to determine the clearance of the glass...article 1986