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Larbi, J.A. (author), van Hees, R.P.J. (author), TNO Bouw (author)The Dutch National Monument in Amsterdam, a World War-II memorial structure, was built with an outer face consisting of slabs of travertine. In 1995, the masonry structure forming the core of the monument showed severe deterioration. In order to determine the actual cause of deterioration and to advise on the most suitable method of restoration,...article 1999
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Wang, M. (author), Hoekstra, S. (author), van Bergen, S. (author), Lamers, G.E.M. (author), Oppedijk, B.J. (author), van der Heijden, M.W. (author), de Priester, W. (author), Schilperoort, R.A. (author), Centrum voor Plantenfysiologisch Onderzoek (author)Intra-nucleosomal cleavage of DNA into fragments of about 200 bp was demonstrated to occur in developing anthers, in which microspores had developed into the mid-late to late uni-nucleate stage in situ, i.e. at the verge of mitosis. The same was observed, but to a much larger extent, if these anthers were pre-treated by a hyper-osmotic shock....article 1999
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Kirev, T. (author), Woutersen, R.A. (author), Kiril, A. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The effects of a diet enriched with 25% raw soya bean flour (RSF) on the pancreas and on the avian retrovirus Pts 56-induced pancreatic carcinogenesis in guinea fowl were studied. It has been shown that prolonged RSF feeding of new-hatched virus-infected and uninfected guinea fowl-poults induced enlargement of the pancreas, which was less...article 1999
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Brocken, H.J.P. (author), Larbi, J.A. (author), Pel, L. (author), van der Pers, N.M. (author), TNO Bouw (author)The formation of cured mortar structure in masonry was studied using multiple experimental techniques. Starting with fresh mortar, nuclear magnetic resonance (NMR) was used to measure the water extraction during brick laying. After curing, the composition of cured mortar was investigated with polarizing and fluorescent microscopy (PFM) and X-Ray...article 1999
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van Rozendaal, B.A.W.M. (author), van de Lest, C.H.A. (author), van Eijk, M. (author), van Golde, L.M.G. (author), Voorhout, W.F. (author), van Helden, H.P.M. (author), Haagsman, H.P. (author)Collectins are carbohydrate binding proteins that are implicated in innate host defense. The lung collectins, surfactant proteins A and D (SP-A and SP-D), bind a variety of pathogens in vitro and influence phagocytosis by alveolar macrophages. In this report we show that SP-D binds endotoxin (lipopolysaccharide, LPS) in vivo in a rat model of...article 1999
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Bär, A. (author), Lina, B.A.R. (author), de Groot, D.M.G. (author), de Bie, B. (author), Appel, M.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)D-Tagatose is an incompletely absorbed ketohexose (stereoisomer of D-fructose) which has potential as an energy-reduced alternative sweetener. In an earlier 90-day toxicity study, rats fed diets with 10, 15 and 20% D-tagatose exhibited increased liver weights, but no histopathological alterations. To determine whether there might be any...article 1999
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TNO Preventie en Gezondheid (author), Mensenkamp, A.R. (author), Jong, M.C. (author), van Goor, H. (author), van Luyn, M.J.A. (author), Bloks, V. (author), Havinga, R. (author), Voshol, P.J. (author), Hofker, M.H. (author), van Dijk, K.W. (author), Havekes, L.M. (author), Kuipers, F. (author)ApoE-deficient mice on low fat diet show hepatic triglyceride accumulation and a reduced very low density lipoprotein (VLDL) triglyceride production rate. To establish the role of apoE in the regulation of hepatic VLDL production, the human APOE3 gene was introduced into apoE-deficient mice by cross-breeding with APOE3 transgenics (APOE3/apoe-/-...article 1999
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Konttinen, Y.T. (author), Halinen, S. (author), Hanemaaijer, R. (author), Sorsa, T. (author), Hietanen, J. (author), Ceponis, A. (author), Xu, J.W. (author), Manthorpe, R. (author), Whittington, J. (author), Larsson, Å. (author), Salo, T. (author), Kjeldsen, L. (author), Stenman, U.-H. (author), Eisen, A.Z. (author), Gaubius Instituut TNO (author)Type IV collagenases/gelatinases (matrix metalloproteinases MMP-2 and MMP-9) in labial salivary glands (LSG) and saliva in Sjogren's syndrome (SS) and healthy controls were studied. Zymograms and Western blots disclosed that SS saliva contained 92/82 kD MMP-9/type IV collagenase duplex. Specific activity measurement disclosed 53.1 ± 9.8 U/mg...article 1998
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Vyth-Dreese, F.A. (author), Boot, H. (author), Dellemijn, T.A.M. (author), Majoor, D.M. (author), Oomen, L.C.J.M. (author), Laman, J.D. (author), van Meurs, M. (author), de Weger, R.A. (author), de Jong, D. (author), TNO Preventie en Gezondheid (author)Costimulatory molecules are essential in cognate interactions between T and B lymphocytes. To study the prerequisites of functional interactions between malignant B cells and intermingled T cells in B-cell non-Hodgkin's lymphomas (B-NHL), we examined the expression of CD40, CD80 and CD86 and their ligands CD40 ligand (CD40L, CD154), CD28 and...article 1998
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Lupu, C. (author), Goodwin, C.A. (author), Westmuckett, A.D. (author), Emeis, J.J. (author), Scully, M.F. (author), Kakkar, V.V. (author), Lupu, F. (author), Gaubius Instituut TNO (author)Tissue factor pathway inhibitor (TFPI), the main downregulator of the procoagulant activity of tissue factor·factor VIIa complex, locates in human endothelial cells (EC) in culture as well-defined clusters uniformly distributed both on the cell surface and intracellularly. We here demonstrate by immunofluorescence that TFPI colocalizes in EC...article 1997
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Beckers, A.L.D. (author), de Bruijn, W.C. (author), Cleton-Soeteman, M.I. (author), van Eijk, H.G. (author), Gelsema, E.S. (author), TNO Fysisch en Elektronisch Laboratorium (author)Reliable iron concentration data can be obtained by quantitative analyses of image sequences, acquired by electron spectroscopic imaging. A number of requirements are formulated for the successful application of this recently developed in situ quantitative type of analysis. A demonstration of the procedures is given. By application of the...article 1997
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Scherphof, G.L. (author), Velinova, M. (author), Kamps, J. (author), Donga, J. (author), van der Want, H. (author), Kuipers, F. (author), Havekes, L. (author), Daemen, T. (author), TNO Preventie en Gezondheid (author)The authors's recent work on matters pertinent to the in vivo processing of systemically administered liposomes is reviewed. particular emphasis is given to factors influencing blood clearance rates, hepatic and splenic uptake and intrahepatic as well as intrasplenic distribution. In addition to size, liposomal composition plays a crucial role...article 1997
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Gerritsen, H.C. (author), Sanders, R. (author), Draaijer, A. (author), Ince, C. (author), Levine, Y.K. (author), TNO Milieu, Energie en Procesinnovatie (author)The usefulness of the fluorescent probe ruthenium tris(2,2′-dipyridyl) dichloride hydrate (RTDP) for the quantitative imaging of oxygen in single cells was investigated utilizing fluorescence life-time imaging. The results indicate that the fluorescence behavior of RTDP in the presence of oxygen can be described by the Stern-Volrner equation....article 1997
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Gaubius Instituut TNO (author), Sakharov, D.V. (author), Plow, E.F. (author), Rijken, D.C. (author)The precursor of plasma carboxypeptidase B (pCPB) also known as thrombin-activable fibrinolysis inhibitor can be converted by thrombin to an active enzyme capable of eliminating C-terminal Lys- and Arg-residues from proteins. The activation is about 1000-fold more efficient in the presence of thrombomodulin (TM). We investigated the...article 1997
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Prins Maurits Laboratorium TNO (author), Bouma, R.H.B. (author), Nauta, W.J. (author), Arnauts, J.E.F. (author), van den Boomgaard, T. (author), Steuten, J.M. (author), Strathmann, H. (author)In the production of polyethylene foams by extrusion with alkanes as a blowing agent, significant changes in the dimensions of extruded products are encountered. The dimensional stability of a foam with a structure of closed cells is improved by blending the polymer with small amounts of a low molecular weight additive. It is believed that the...article 1997
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TNO Bouw (author), Larbi, J.A. (author), Heijnen, W.M.M. (author)This article presents the results of a microscopic analysis to determine the cement content of five samples of hardened concrete prepared with portland pozzolanic cement and crushed limestone as part of the aggregate. The volume fraction of the coarse aggregate was determined macroscopically from polished plates of the concrete samples. The...article 1997
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Visser, C.J.T. (author), van Garderen-Hoetmer, A. (author), Klijn, J.G.M. (author), Foekens, J.A. (author), Woutersen, R.A. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)In a previous short-term study (4 months) we found that Sandostatin, when administered prophylactically, inhibited growth of putative pre-neoplastic ductular lesions induced in hamster pancreas by N-nitrosobis(2-oxopropyl)amine (BOP), but not of acinar lesions induced in rat pancreas by azaserine. The present long-term (12 months) study was...article 1996
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- van Bree, J.L.M.J. (author), Duvalois, W. (author), Somogy, P. (author), Prins Maurits Laboratorium TNO (author) conference paper 1996
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Visser, M.J.T. (author), van Lennep, A.C.D. (author), van Bockel, J.H. (author), van Hinsbergh, V.W.M. (author), van der Keur, M. (author), Hermans, J. (author), Gaubius Instituut TNO (author)For in vitro evaluation of functional properties of endothelial cells seeded on synthetic vascular prostheses accurate and reproducible quantification of cells is mandatory. Comparison of these properties with those resulting from other studies requires correlation of the functional parameters to reliably counted cell numbers. The accuracy of...article 1996
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Gaubius Instituut TNO (author), Sakharov, D.V. (author), Lijnen, H.R. (author), Rijken, D.C. (author)Staphylokinase (STA), a protein of bacterial origin, induces highly fibrin-specific thrombolysis both in human plasma in vitro and in pilot clinical trials. Using fluorescence microscopy, we investigated the spatial distribution of fluorescein isothiocyanate (FITC)-labeled STA during lysis of a plasma clot and its binding to purified fibrin...article 1996