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Pieterse, B. (author), Jellema, R.H. (author), van der Werf, M.J. (author), TNO Kwaliteit van Leven (author)
Messenger RNA levels change on a minutes scale due to both degradation and de novo transcription. Consequently, alterations in the transcript profiles that are not representative for the condition of interest are easily introduced during sample harvesting and work-up. In order to avoid these unwanted changes we have validated a - 45°C methanol...
article 2006