Searched for: subject:"Microarray"
(1 - 20 of 73)

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Matualatupauw, J.C. (author), O'Grada, C. (author), Hughes, M.F. (author), Roche, H.M. (author), Afman, L.A. (author), Bouwman, J. (author)
Scope: Several studies have examined the whole-genome gene expression response in blood cells to high-fat challenges with differing results. The study aims to identify consistently up- or downregulated genes and pathways in response to a high-fat challenge using several integration methods. Methods and results: Three studies measuring the gene...
article 2019
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Dols, J.A.M. (author), Molenaar, D. (author), van der Helm, J.J. (author), Caspers, M.P.M. (author), de Kat Angelino-Bart, A. (author), Schuren, F.H.J. (author), Speksnijder, A.G.C.L. (author), Westerhoff, H.V. (author), Richardus, J.H. (author), Boon, M.E. (author), Reid, G. (author), de Vries, H.J.C. (author), Kort, R. (author)
Background: To date, women are most often diagnosed with bacterial vaginosis (BV) using microscopy based Nugent scoring or Amsel criteria. However, the accuracy is less than optimal. The aim of the present study was to confirm the identity of known BV-associated composition profiles and evaluate indicators for BV using three molecular methods....
article 2016
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Matualatupauw, J.C. (author), Radonjic, M. (author), van de Rest, O. (author), de Groot, L.C.P.G.M. (author), Geleijnse, J.M. (author), Müller, M. (author), Afman, L.A. (author)
Scope: People who carry the apolipoprotein E4 (APOE4) single nucleotide polymorphism have an increased risk of cardiovascular disease (CVD). Fish-oil supplementation may help in the prevention of CVD, though interindividual differences in the response to n-3 PUFAs have been observed. We aimed to assess the impact of APOE genotype on peripheral...
article 2016
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Eijssen, L.M.T. (author), Goelela, V.S. (author), Kelder, T. (author), Adriaens, M.E. (author), Evelo, C.T. (author), Radonijc, M. (author)
Background Illumina whole-genome expression bead arrays are a widely used platform for transcriptomics. Most of the tools available for the analysis of the resulting data are not easily applicable by less experienced users. ArrayAnalysis.org provides researchers with an easy-to-use and comprehensive interface to the functionality of R and...
article 2015
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van der Tuin, S.J. (author), Kühnast, S. (author), Berbée, J. (author), Verschuren, L. (author), Pieterman, E.J. (author), Havekes, L.M. (author), van der Hoorn, J.W. (author), Rensen, P.C. (author), Jukema, J.W. (author), Princen, H.M. (author), Willems van Dijk, K. (author), Wang, Y. (author)
Recently, we showed in APOE*3-Leiden.CETP mice that anacetrapib attenuated atherosclerosis development by reducing (V)LDL-C rather than by raising HDL-C. Here, we investigated the mechanism by which anacetrapib reduces (V)LDL-C and whether this effect was dependent on the inhibition of CETP. APOE*3-Leiden.CETP mice were fed a Western type diet...
article 2015
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Mariman, R. (author), Tielen, F. (author), Koning, F. (author), Nagelkerken, L. (author)
Background: Probiotic bacteria may render mice resistant to the development of various inflammatory and infectious diseases. Objective: This study aimed to identify mechanisms by which probiotic bacteria may influence intestinal immune homeostasis in noninflammatory conditions. Methods: The effect of VSL#3, a mixture of 8 probiotic bacteria, on...
article 2015
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Grinberg, M. (author), Stöber, R.M. (author), Edlund, K. (author), Rempel, E. (author), Godoy, P. (author), Reif, R. (author), Widera, A. (author), Madjar, K. (author), Schmidt-Heck, W. (author), Marchan, R. (author), Sachinidis, A. (author), Spitkovsky, D. (author), Hescheler, J. (author), Carmo, H. (author), Arbo, M.D. (author), van de Water, B. (author), Wink, S. (author), Vinken, M. (author), Rogiers, V. (author), Escher, S. (author), Hardy, B. (author), Mitic, D. (author), Myatt, G. (author), Waldmann, T. (author), Mardinoglu, A. (author), Damm, G. (author), Seehofer, D. (author), Nüssler, A. (author), Weiss, T.S. (author), Oberemm, A. (author), Lampen, A. (author), Schaap, M.M. (author), Luijten, M. (author), van Steeg, H. (author), Thasler, W.E. (author), Kleinjans, J.C.S. (author), Stierum, R.H. (author), Leist, M. (author), Rahnenführer, J. (author), Hengstler, J.G. (author)
A long-term goal of numerous research projects is to identify biomarkers for in vitro systems predicting toxicity in vivo. Often, transcriptomics data are used to identify candidates for further evaluation. However, a systematic directory summarizing key features of chemically influenced genes in human hepatocytes is not yet available. To bridge...
article 2014
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Gruben, B.S. (author), Zhou, M. (author), Wiebenga, A. (author), Ballering, J. (author), Overkamp, K.M. (author), Punt, P.J. (author), de Vries, R.P. (author)
The genome of the filamentous fungus Aspergillus niger is rich in genes encoding pectinases, a broad class of enzymes that have been extensively studied due to their use in industrial applications. The sequencing of the A. niger genome provided more knowledge concerning the individual pectinolytic genes, but little is known about the regulatory...
article 2014
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Ladirat, S.E. (author), Schuren, F.H.J. (author), Schoterman, M.H.C. (author), Nauta, A. (author), Gruppen, H. (author), Schols, H.A. (author)
Prebiotics are considered to have potential to reduce disturbances in the gut microbiota induced by antibiotics. Results in literature are, however, not consistent. The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota...
article 2014
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Vaessen, S.F. (author), Bruysters, M.W. (author), Vandebriel, R.J. (author), Verkoeijen, S. (author), Bos, R. (author), Krul, C.A.M. (author), Akkermans, A.M. (author)
Pertussis vaccines are routinely administered to infants to protect them from whooping cough. Still, an adequate safety test for pertussis toxin (PT), one of the main antigens in these vaccines, is not available. The histamine sensitization test is currently the only assay accepted by regulatory authorities to test for the absence of active PT...
article 2014
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Radonjic, M. (author), Cappaert, N.L.M. (author), de Vries, E.F.J. (author), de Esch, C.E.F. (author), Kuper, F.C. (author), van Waarde, A. (author), Dierckx, R.A.J.O. (author), Wadman, W.J. (author), Wolterbeek, A.P.M. (author), Stierum, R.H. (author), de Groot, D.M.G. (author)
Maternal exposure to the neurotoxin methylmercury (MeHg) has been shown to have adverse effects on neural development of the offspring in man. Little is known about the underlying mechanisms by which MeHg affects the developing brain. To explore the neurodevelopmental defects and the underlying mechanism associated with MeHg exposure, the...
article 2013
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van der Hoeven-Hangoor, E. (author), van der Vossen, J.M.B.M. (author), Schuren, F.H.J. (author), Verstegen, M.W.A. (author), de Oliveira, J.E. (author), Montijn, R.C. (author), Hendriks, W.H. (author)
Microbiota plays a role in the release and absorption of nutrients from feed components, thereby affecting digesta composition and moisture content of the excreta. The objective of the current study was to determine the effects of 5 different diets varying in ingredients (medium-chain fatty acids, nonstarch polysaccharides, and starch) on the...
article 2013
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Ladirat, S.E. (author), Schols, H.A. (author), Nauta, A. (author), Schoterman, M.H.C. (author), Keijser, B.J.F. (author), Montijn, R.C. (author), Gruppen, H. (author), Schuren, F.H.J. (author)
Antibiotic treatments can lead to a disruption of the human microbiota. In this in-vitro study, the impact of antibiotics on adult intestinal microbiota was monitored in a new high-throughput approach: a fermentation screening-platform was coupled with a phylogenetic microarray analysis (Intestinal-chip). Fecal inoculum from healthy adults was...
article 2013
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Tapp, H.S. (author), Radonjic, M. (author), Kemsley, E.K. (author), Thissen, U. (author)
Genomics-based technologies produce large amounts of data. To interpret the results and identify the most important variates related to phenotypes of interest, various multivariate regression and variate selection methods are used. Although inspected for statistical performance, the relevance of multivariate models in interpreting biological...
article 2012
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Euser, S.M. (author), Nagelkerke, N.J. (author), Schuren, F. (author), Jansen, R. (author), den Boer, J.W. (author)
Background: Legionella, the causative agent for Legionnaires' disease, is ubiquitous in both natural and man-made aquatic environments. The distribution of Legionella genotypes within clinical strains is significantly different from that found in environmental strains. Developing novel genotypic methods that offer the ability to distinguish...
article 2012
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Mariman, R. (author), Kremer, S.H.A. (author), van Erk, M. (author), Lagerweij, T. (author), Koning, F. (author), Nagelkerken, L. (author)
Background: Host-microbiota interactions in the intestinal mucosa play a major role in intestinal immune homeostasis and control the threshold of local inflammation. The aim of this study was to evaluate the efficacy of probiotics in the recurrent trinitrobenzene sulfonic acid (TNBS)-induced colitis model and gain more insight into protective...
article 2012
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Dols, J.A.M. (author), Reid, G. (author), Kort, R. (author), Schuren, F.H.J. (author), Tempelman, H. (author), Bontekoe, T.R. (author), Korporaal, H. (author), van der Veer, E.M. (author), Smit, P.W. (author), Boon, M.E. (author)
Vaginal lactobacilli assessed by PCR-based microarray and PCR-based genotyping of HPV in South African women at risk for HIV and BV. Vaginal lactobacilli can be defined by microarray techniques in fixed cervical samples of South African women. Cervical brush samples suspended in the coagulant fixative BoonFix of one hundred women attending a...
article 2012
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Boesten, R. (author), Schuren, F. (author), Wind, R.D. (author), Knol, J. (author), de Vos, W.M. (author)
A total of 20 Bifidobacterium strains were isolated from fecal samples of 4 breast- and bottle-fed infants and all were characterized as Bifidobacterium breve based on 16S rRNA gene sequence and metabolic analysis. These isolates were further characterized and compared to the type strains of B. breve and 7 other Bifidobacterium spp. by...
article 2011
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van der Vossen, J. (author), Keijser, B. (author), Schuren, F. (author), Nocker, A. (author), Montijn, R. (author)
Thermophilic Campylobacter species are the most common cause of gastroenteritis in developed countries. Campylobacter spp. are ubiquitous in nature and widespread in livestock. Infections occur sporadically, and are believed to occur through consumption of contaminated meat products and from environmental sources. DNA-based techniques are...
conference paper 2011
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Boesten, R.J. (author), Schuren, F.H.J. (author), Willemsen, L.E.M. (author), Vriesema, A. (author), Knol, J. (author), de Vos, W.M. (author)
To provide insight in the molecular basis for intestinal host-microbe interactions, we determined the genome-wide transcriptional response of human intestinal epithelial cells following exposure to cells of Bifidobacterium breve. To select an appropriate test system reflecting inflammatory conditions, the responsiveness to TNF-a was compared in...
article 2011
Searched for: subject:"Microarray"
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