A new therapeutic approach to treat psoriasis by inhibition of fatty acid oxidation by Etomoxir

Background: The dogma in psoriasis is that due to pathogen-induced inflammatory responses, an autoreactive immune response is induced that leads to tissue destruction. However, this model might be too simplistic. Literature data suggest that the expression of enzymes crucial for fatty acid oxidation is upregulated in the skin of patients with...

Removal of UV-induced DNA lesions in mouse epidermis soon after irradiation

Induction and removal of cyclobutane thymine dimers and (6-4)photoproducts were studied in epidermal DNA isolated from UV-exposed hairless mice. For the detection of DNA damage, lesion-specific monoclonal antibodies were used in an immunoslotblot assay. Following the exposure of mice to 3.0 kJ m-2 UV-B, substantial removal of both thymine dimers...

Immunochemical detection of cyclobutane thymine dimers in epidermal Langerhans cells of UV-B irradiated hairless mice

An immunocytochemical method was developed to study in vivo induction and removal of DNA damage in a specific cell population in the epidermis of hairless mice after ultraviolet B (UVB) exposure: the immunocompetent antigen-presenting Langerhans cells. To this aim, Ia+ cells, which are representative for epidermal Langerhans cells, were compared...

Detection of thymine dimers in suprabasal and basal cells of chronically UV-B exposed hairless mice

An immunocytochemical method was developed to study induction and removal of DNA damage in specific cell populations in the epidermis of hairless mice during chronic ultraviolet (UV) exposure. Identification of mouse suprabasal cells was performed with an immunoperoxidase stain. This stain was shown not to affect the fluorescent nuclear stains,...