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Pieterse, B. (author), Jellema, R.H. (author), van der Werf, M.J. (author), TNO Kwaliteit van Leven (author)Messenger RNA levels change on a minutes scale due to both degradation and de novo transcription. Consequently, alterations in the transcript profiles that are not representative for the condition of interest are easily introduced during sample harvesting and work-up. In order to avoid these unwanted changes we have validated a - 45°C methanol...article 2006
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Jeurissen, S.M.F. (author), Claassen, F.W. (author), Havlik, J. (author), Bouwmans, E.E. (author), Cnubben, N.H.P. (author), Sudhölter, E.J.R. (author), Rietjens, I.M.C.M. (author), van Beek, T.A. (author), TNO Kwaliteit van Leven (author)An on-line HPLC screening method for detection of inhibitors of human cytochrome P450 1A2 in extracts was developed. HPLC separation of extracts is connected to a continuous methoxyresorufin-O-demethylation (MROD) assay in which recombinant human P450 1A2 converts methoxyresorufin to its fluorescent metabolite resorufin. The system was tested...article 2007
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van Lune, F.S. (author), Nijssen, L.M. (author), Linssen, J.P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)Absorption aspects of flavours and chemicals are important quality criteria when plastic bottles are reused or recycled. In the present study absorption of methanol and toluene by polyester-based bottles (PET and PEN) was studied at different temperatures in the range 20-40°C for a period of 16 weeks with two different ways of exposure: one...article 1997
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Boon, J. (author), van Kampen, J. (author), Hoogendoorn, R. (author), Tanase, S. (author), van Berkel, F.P.F. (author), van Sint Annaland, M. (author)Acidic γ-Al2O3 is an active catalyst for the dehydration of methanol to dimethyl ether (DME). However, the produced steam reduces the activity. In this work, the influence of the exposure of γ-Al2O3 to steam on the catalytic activity for methanol dehydration has been determined. At 250 °C and increasing stream partial pressure the conversion of...article 2019
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Coulier, L. (author), Bas, R. (author), Jespersen, S. (author), Verheij, E. (author), van der Werf, M.J. (author), Hankemeier, T. (author), TNO Kwaliteit van Leven TNO Voeding (author)We have developed an analytical method, consisting of ion-pair liquid chromatography coupled to electrospray ionization mass spectrometry (IP-LC-ESI-MS), for the simultaneous quantitative analysis of several key classes of polar metabolites, like nucleotides, coenzyme A esters, sugar nucleotides, and sugar bisphosphates. The use of the ion-pair...article 2006
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Clary, J.J. (author), Feron, V.J. (author), van Velthuijsen, J.A. (author), TNO Voeding (author)Organo psycho syndrome (OPS) or chronic toxic encephalopathy (CTE) is a neurotoxic condition reported following long-term exposure to paints containing organic solvent and to other solvents. Lactate esters are finding wider use as solvents. Lactate esters have been well studied in standard toxicity tests, but specific neurotoxicity studies have...article 2001
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de Groot, D.M.G. (author), Bos-Kuijpers, M.H.M. (author), Kaufmann, W.S.H. (author), Lammers, J.H.C.M. (author), O'Callaghan, J.P. (author), Pakkenberg, B. (author), Pelgrim, M.T.M. (author), Waalkens-Berendsen, I.D.H. (author), Waanders, M.M. (author), Gundersen, H.J.J. (author), TNO Kwaliteit van Leven (author)Our aim was to investigate a model of the morphologic approach proposed in guidelines for developmental neurotoxicity testing (DNT). Hereto, a limited DNT study [EPA Health Effects Test Guidelines OPPTS 870.6300, 1996a. Developmental Neurotoxicity Study "Public Draft", United States Environmental Protection Agency; Prevention, Pesticides and...article 2005
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Mol, H.G.J. (author), van Dam, R.C.J. (author), Vreeken, R.J. (author), Steijger, O.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A straightforward and reliable method was developed for the determination of chlormequat in pears by liquid chromatography/mass spectrometry (LC/MS). Water and methanol were compared as extraction solvents. Because no significant differences in extraction efficiency or repeatability were found, water was chosen as the extraction solvent. The...article 2000
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Koopman, F.W. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author), TNO Kwaliteit van Leven (author)The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to efficiently utilize the C1 compounds methanol and formaldehyde as auxiliary substrate. The hps and phi genes of Bacillus brevis, encoding two key steps of the ribulose monophosphate (RuMP) pathway, were introduced to construct a pathway for the metabolism of the toxic...article 2009
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Peinado, C. (author), Liuzzi, D. (author), Sanchis, A. (author), Pascual, L. (author), Peña, M.A. (author), Boon, J. (author), Rojas, S. (author)The synthesis of methanol from biomass-derived syngas can be challenging because of the high CO2 content in the bio-syngas, resulting in lower kinetics and higher catalyst deactivation. This work explores the in situ pre-treatment of a CO2-rich syngas with a CO2/CO ratio equal to 1.9 through the reverse-water gas shift reaction with the aim of...article 2021
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Jónasdóttir, H.S. (author), Ioan-Facsinay, A. (author), Kwekkeboom, J. (author), Brouwers, H. (author), Zuurmond, A.M. (author), Toes, R. (author), Deelder, A.M. (author), Giera, M. (author)Here we present an advanced platform for the analysis of specialized pro-resolving mediators (SPM), including a set of pro-inflammatory lipids and respective biochemical pathway markers. The platform is characterized by a largely simplified sample preparation protocol, employing methanol protein-precipitation instead of solid phase extraction....article 2014
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Oliveira, C.C.N. (author), Rochedo, P.R.R. (author), Bhardwaj, R. (author), Worrell, E. (author), Szklo, A. (author)The urgency with which the world economy needs to be decarbonized could lead to the emergence of regions with the capacity to produce renewable feedstock such as biomass. The competitiveness of these regions could result from their ability to produce high value-added chemicals at the lowest cost. The biomass embodied in a chemical product could...article 2019
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Gaubius instituut TNO (author), Havekes, L.M. (author), de Knijff, P. (author), Beisiegel, U. (author), Havinga, J. (author), Smit, M. (author), Klasen, E. (author)A new method for the apolipoprotein E phenotyping has been developed. The method is based on isoelectric focusing of either delipidated or guanidine-HCl-treated serum or plasma in a horizontal slab gel system followed by immunoblotting using either polyclonal or monoclonal anti-apolipoprotein E antibodies as first antibody. Apolipoprotein E...article 1987
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Gentile, G. (author), Bonalumi, D. (author), Pieterse, J.A.Z. (author), Sebastiani, F. (author), Lucking, L.E. (author), Manzolini, G. (author)The iron and steel industry accounts for 6 % of the global CO2 emissions and it is one of the main hard-to-abate sectors that must be un-locked to reach climate neutrality in the coming decades. The objective of this work is to assess the economics of the FReSMe (From Residual Steel gases to Methanol) process for reducing the carbon footprint of...article 2022
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Ghosh, M.K. (author), Howard, M.S. (author), Dussan, K. (author), Dooley, S. (author)A detailed systematic theoretical study of the mechanism of the homogeneous Brønsted-acid catalysis of d-glucose in aqueous solution phase ("acid hydrolysis") is reported. G4MP2 with the SMD solvation model at B3LYP/6-31G(2df,p) are employed to compute the free energies of the molecular and ionic species pertaining to the isomerization,...article 2019
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Medisch Biologisch Laboratorium TNO (author), Jongen, M.J.M. (author), Engel, R. (author), Leenheers, L.H. (author)An HPLC method was developed for estimation of dermal exposure of greenhouse workers to the pesticide bupirimate. Chromatography was performed on a cyano-modified silica column with methanol-water (6:4 by volume) containing 5 g/L ammonium sulfate as eluent. UV detection at 310 nm was used for quantitation. Dermal exposure was assessed by letting...article 1992
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Bosklopper, J. (author), Sapra, H. (author), van de Ketterij, R. (author), van Sluijs, W. (author), Bekdemir, C. (author), de Vos, P. (author), Visser, K. (author)The maritime sector is currently facing a serious challenge of reducing emissions to meet the strict international and national emission regulations. In response to this challenge, the industry is initiating several research initiatives focused towards employment of alternative fuels in marine internal combustion engines. One of the promising...conference paper 2020
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Spoelstra, S. (author), Overbeek, J.P. (author), Hugill, J.A. (author)To achieve sustainable development in the chemical industry, there is a need for methodologies to assess the eco-efficiency of alternative and novel technologies. The aim of this project was to use a case study to investigate the application of existing tools to assess eco-efficiency. The case study compared the conventional methanol production...other 2001
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Spoelstra, S. (author)-other 2000
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van Lieshout, P.S. (author), de Jonge, V. (author), Vredeveldt, A.W. (author), Finner, S. (author)Introducing alternative fuels on board has many far stretching implications for ship operators, authorities, fuel suppliers, equipment suppliers, engine manufacturers, ship builders and naval architects. In most cases such parties are not familiar with these new fuels. Hence a need exists for them for a means to quickly familiarize oneself with...report 2020