Searched for: subject:"Methanol"
(1 - 3 of 3)
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Jónasdóttir, H.S. (author), Ioan-Facsinay, A. (author), Kwekkeboom, J. (author), Brouwers, H. (author), Zuurmond, A.M. (author), Toes, R. (author), Deelder, A.M. (author), Giera, M. (author)
Here we present an advanced platform for the analysis of specialized pro-resolving mediators (SPM), including a set of pro-inflammatory lipids and respective biochemical pathway markers. The platform is characterized by a largely simplified sample preparation protocol, employing methanol protein-precipitation instead of solid phase extraction....
article 2014
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Pieterse, B. (author), Jellema, R.H. (author), van der Werf, M.J. (author), TNO Kwaliteit van Leven (author)
Messenger RNA levels change on a minutes scale due to both degradation and de novo transcription. Consequently, alterations in the transcript profiles that are not representative for the condition of interest are easily introduced during sample harvesting and work-up. In order to avoid these unwanted changes we have validated a - 45°C methanol...
article 2006
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Coulier, L. (author), Bas, R. (author), Jespersen, S. (author), Verheij, E. (author), van der Werf, M.J. (author), Hankemeier, T. (author), TNO Kwaliteit van Leven TNO Voeding (author)
We have developed an analytical method, consisting of ion-pair liquid chromatography coupled to electrospray ionization mass spectrometry (IP-LC-ESI-MS), for the simultaneous quantitative analysis of several key classes of polar metabolites, like nucleotides, coenzyme A esters, sugar nucleotides, and sugar bisphosphates. The use of the ion-pair...
article 2006