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TNO Preventie en Gezondheid (author), van der Slot, A.J. (author), Zuurmond, A.M. (author), Bardoel, A.F.J. (author), Wijmenga, C. (author), Pruijs, H.E.H. (author), Sillence, D.O. (author), Brinckmann, J. (author), Abraham, D.J. (author), Black, C.M. (author), Verzijl, N. (author), de Groot, J. (author), Hanemaaijer, R. (author), te Koppele, J.M. (author), Huizinga, T.W.J. (author), Bank, R.A. (author)The hallmark of fibrotic processes is an excessive accumulation of collagen. The deposited collagen shows an increase in pyridinoline cross-links, which are derived from hydroxylated lysine residues within the telopeptides. This change in cross-linking is related to irreversible accumulation of collagen in fibrotic tissues. The increase in...article 2003
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TNO Preventie en Gezondheid (author), Tolboom, T.C.A. (author), Pieterman, E. (author), van der Laan, W.H. (author), Toes, R.E.M. (author), Huidekoper, A.L. (author), Nelissen, R.G.H.H. (author), Breedveld, F.C. (author), Huizinga, T.W.J. (author)Background: Matrix metalloproteinases (MMPs) have a pivotal role in the destruction of cartilage in rheumatoid arthritis (RA), which is mediated by the fibroblast-like synoviocytes (FLS). Objective: To examine the in vitro invasiveness of synoviocytes obtained from inflamed joints of patients with arthritis in relation to the expression of MMP 1...article 2002
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Boxel van-Dezaire, A.H.H. (author), Smits, M. (author), Trigt van-Hoff, S.C.J. (author), Killestein, J. (author), Houwelingen, J.C van (author), Polman, C.H (author), Nagelkerken, L. (author), TNO Preventie en Gezondheid (author)Little is known about the involvement of cytokines in the pathogenesis of primary progressive (PP) multiple sclerosis (MS). We evaluated in this cross-sectional study whether IL-18, IL-12p35, IL-12p40, TNF-α, IFN-γ, IL-10, IL-4, TGF-β, IL-12Rβ1, and IL-12Rβ2 mRNA expression in unstimulated white blood cells showed significant differences between...article 2001
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Bajramović, J.J. (author), Geutskens, S.B. (author), Bsibsi, M. (author), Boot, M. (author), Hassankhan, R. (author), Verhulst, K.C. (author), van Noort, J.M. (author), Div. Immunological and Infect. Dis., TNO Prevention and Health, PO Box 2215, 2301 CE Leiden, Netherlands (author)We describe a reverse transcriptase-polymerase chain reaction method for the semiquantitative detection of mRNAs encoding the human heat shock proteins αβ-crystallin, Hsp27, and Hsp60. The method involves the coamplification of cellular mRNA-derived cDNA with a dilution series of a competitor fragment (internal standard), using 1 primer pair...article 2000
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Conesa, A. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)To get insight into the limiting factors existing for the efficient production of fungal peroxidase in filamentous fungi, the expression of the Phanerochaete chrysosporium lignin peroxidase H8 (lipA) and manganese peroxidase (MnP) H4 (mnp1) genes in Aspergillus niger has been studied. For this purpose, a protease-deficient A. niger strain and...article 2000
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Boxel van-Dezaire, A.H.H. (author), Trigt van-Hoff, S.C.J. (author), Killestein, J. (author), Schrijver, H.M. (author), van Houwelingen, J.C. (author), Polman, C.H. (author), Nagelkerken, L. (author), TNO Preventie en Gezondheid (author)Interferon (IFN)-β treatment is effective in relapsing-remitting multiple sclerosis (RR-MS) via an as yet unidentified mechanism. In the present study, we investigated whether the expression of messenger RNA (mRNA) encoding the interleukin (IL)-12 subunits p40 and p35, IL-12 receptor chains, IL-18, tumor necrosis factor-α (TNFα), IFNγ, IL-10, IL...article 2000
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Lake, B.G. (author), Rumsby, P.C. (author), Price, R.J. (author), Cunninghame, M.E. (author), TNO BIBRA TNO Kwaliteit van Leven (author)The objective of this study was to evaluate species differences in the hepatic effects of three potent rodent peroxisome proliferators, namely methylclofenapate (MCP), ciprofibrate (CIP) and Wy-14,643 (WY), particularly with respect to effects on replicative DNA synthesis and transforming growth factor-β1 (TGF-β1) gene expression. Male Sprague...article 2000
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Griffiths, R. (author), Grieve, A. (author), Scollon, J. (author), Scott, M. (author), Williams, C. (author), Meredith, C. (author), TNO Kwaliteit van Leven TNO Bibra (author)Using primary cultures of mouse cerebellar granule cells as an in vitro model system, it has been demonstrated that different profiles of temporal expression of the c-fos proto-oncogene are observed under non-excitotoxic and excitotoxic conditions. A ratio has been derived previously for the steady- state level of c-fos mRNA after 30 min and 240...article 2000
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Bajramović, J.J. (author), Bsibsi, M. (author), Geutskens, S.B. (author), Hassankhan, R. (author), Verhulst, K.C. (author), Stege, G.J.J. (author), de Groot, C.J.A. (author), van Noort, J.M. (author), TNO Preventie en Gezondheid (author)Various lines of evidence suggest a close relationship between heat shock proteins (hsp) and several autoimmune diseases such as arthritis, diabetes and multiple sclerosis. While enhanced expression of hsp in autoimmune diseases is often regarded as a non-specific bystander effect of the inflammatory process, surprisingly little is known on hsp...article 2000
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Ngiam, C. (author), Jeenes, D.J. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Archer, D.B. (author)Protein disulfide isomerase (PDI) is important in assisting the folding and maturation of secretory proteins in eukaryotes. A gene, pdiA, encoding PDIA was previously isolated from Aspergillus niger, and we report its functional characterization here. Functional analysis of PDIA showed that it catalyzes the refolding of denatured and reduced...article 2000
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van Mechelen, J.R. (author), Schuurink, R.C. (author), Smits, M. (author), Graner, A. (author), Douma, A.C. (author), Sedee, N.J.A. (author), Schmitt, N.F. (author), Valk, B.E. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Two full-length lipoxygenase cDNA sequences (LoxB and LoxC) from barley (Hordeum distichum cv. L. Triumph) are described. The cDNAs share high homology with the barley LoxA cDNA. Southern blotting experiments indicate single copy numbers of the three lipoxygenase genes. RFLP mapping revealed the presence of single lipoxygenase loci. LoxA and...article 1999
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Schuren, F.H.J. (author), Wessels, J.G.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)GPD regulatory sequences were used to express a phleomycin resistance gene (Sh ble) in Schizophyllum commune, resulting in high numbers of phleomycin-resistant transformants. Attempts to express heterologous genes coding for hygromycin B phosphotransferase (hph), aminoglycoside phosphotransferase (apt), β-glucuronidase (uidA) and β-galactosidase...article 1998
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Monshouwer, M. (author), van 't Klooster, G.A.E. (author), Nijmeijer, S.M. (author), Witkamp, R.F. (author), van Miert, A.S.J.P.A.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Despite the fact that pigs are increasingly used in pharmacological and toxicological studies, knowledge on the enzymes which metabolize xenobiotics, in particular cytochrome P450 (CYP) enzymes, in pigs is still very limited. Primary cultures of pig hepatocytes were used to characterize CYP enzymes. The characterization was performed at the...article 1998
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Sherratt, P.J. (author), Manson, M.M. (author), Thomson, A.M. (author), Hissink, E.A.M. (author), Neal, G.E. (author), van Bladeren, P.J. (author), Green, T. (author), Hayes, J.D. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A characteristic feature of the class Theta glutathione S-transferase (GST) T1-1 is its ability to activate dichloromethane and dibromoethane by catalysing the formation of mutagenic conjugates. The level of the GSTT1 subunit within tissues is an important determinant of susceptibility to the carcinogenic effects of these dihaloalkanes. In the...article 1998
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Gaubius Instituut TNO (author), van Vlijmen, B.J.M. (author), Pearce, N.J. (author), Bergö, M. (author), Staels, B. (author), Yates, J.W. (author), Gribble, A.D. (author), Bond, B.C. (author), Hofker, M.H. (author), Havekes, L.M. (author), Groot, P.H.E. (author)Apolipoprotein (APO) E*3-Leiden mice with impaired chylomicron and VLDL (very low density lipoprotein) remnant metabolism display hyperlipidaemia and atherosclerosis. In the present study, these mice were used for testing the hypolipidaemic effect of two marketed agents, lovastatin (CAS 75330-75-5) and gemfibrozil (CAS 25812-30-0) as well as a...article 1998
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Beekman, B. (author), Verzijl, N. (author), de Roos, J.A.D.M. (author), Koopman, J.L. (author), Tekoppele, J.M. (author), Leiden, Netherlands Gaubius Instituut TNO (author)Doxycycline is known for its ability to inhibit matrix metalloproteinases (MMPs), a family of enzymes that play a role in cartilage breakdown in arthritides. Its prophylactic effect in reducing joint degradation in osteoarthritis is mainly attributed to this property. In this study, we show that doxycycline exhibits a profound inhibition of...article 1997
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Braakhuis, B.J.M. (author), Klaassen, I. (author), van der Leede, B.M. (author), Cloos, J. (author), Brakenhoff, R.H. (author), Copper, M.P. (author), Teerlink, T. (author), Hendriks, H.F.J. (author), van der Saag, P.T. (author), Snow, G.B. (author)Retinoids can reverse potentially premalignant lesions and prevent second primary tumours in patients with head and neck squamous cell carcinoma (HNSCC). Furthermore, it has been reported that acquired resistance to all-trans retinoic acid (RA) in leukaemia is associated with decreased plasma peak levels, probably the result of enhanced retinoid...article 1997
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van Leeuwen, R.T.J. (author), Quax, P.H.A. (author), Tippins, J.R. (author), Antoniw, J.W. (author), Andreotti, F. (author), Maseri, A. (author), Kluft, C. (author), Sperti, G. (author), Gaubius Instituut TNO (author)Background: Lipopolysaccharide (endotoxin) has been shown to increase the expression of plasminogen activator inhibitor type-1 (PAI-1) in the vessel wall. Endotoxin is known to increase PAI-1 production in endothelial cells, but its action on smooth muscle cells (SMCs) is presently not clear. In this study we determined the effect of endotoxin...article 1996
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Arts, J. (author), Grimbergen, J. (author), Bosma, P.J. (author), Rahmsdorf, H.J. (author), Kooistra, T. (author), Gaubius Laboratory, TNO-PG, Leiden, Netherlands Institut für Genetik, Forschungszentrum Karlsruhe, Germany TNO Preventie en Gezondheid (author)Experiments were designed to clarify the role of c-Jun/c-Fos and of putative phorbol 12-myristate-13-acetate-(PMA)-responsive elements (TREs) in the induction of plasminogen-activator inhibitor 1 (PAI-1) gene transcription in the human hepatoma cell line HepG2 by activators of protein kinase C (PKC). Treatment of HepG2 cells with the phorbol...article 1996
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TNO Nutrition and Food Research (author), van Gemeren, I.A. (author), Beijersbergen, A. (author), Musters, W. (author), Gouka, R.J. (author), van den Hondel, C.A.M.J.J. (author), Verrips, C.T. (author)A synthetic derivative of the cutinase cDNA of Fusarium solani pisi was expressed in Aspergillus awamori using the A. awamori endoxylanase II (exlA) promoter and terminator. The influence of the origin of the pre-sequence and the presence of a pro-sequence on the efficiency of extracellular cutinase production was analyzed in single-copy...article 1996