- document
-
van den Nieuwendijk, A.M.C.H. (author), Benningshof, J.C.J. (author), Wegmann, V. (author), Bank, R.A. (author), te Koppele, J.M. (author), Brussee, J. (author), van der Gen, A. (author), TNO Preventie en Gezondheid (author)A new synthetic route to reduced collagen crosslinks (LNL and HLNL) is described in this report. It enables an enantioselective synthesis of LNL. HLNL was obtained as a mixture of two diastereoisomers. This method also provides the possibility to introduce radio-labels during the synthesis.article 1999
- document
-
van Boxel-Dezaire, A.H.H. (author), Hoff, S.C.J. (author), van Oosten, B.W. (author), Verweij, C.L. (author), Dräger, A.M. (author), Adèr, H.J. (author), van Houwelingen, J.C. (author), Barkhof, F. (author), Polman, C.H. (author), Nagelkerken, L. (author), TNO Preventie en Gezondheid (author)It has been shown that proinflammatory and antiinflammatory cytokines correlate with disease activity in multiple sclerosis (MS). To establish whether such correlations depend on the disease stage, we assessed in a longitudinal fashion the expression of interleukin (IL)-12 (p40 and p35), tumor necrosis factor- 1/4 , interferon-γ, and IL-10 mRNA...article 1999
- document
-
Brocken, H.J.P. (author), Larbi, J.A. (author), Pel, L. (author), van der Pers, N.M. (author), TNO Bouw (author)The formation of cured mortar structure in masonry was studied using multiple experimental techniques. Starting with fresh mortar, nuclear magnetic resonance (NMR) was used to measure the water extraction during brick laying. After curing, the composition of cured mortar was investigated with polarizing and fluorescent microscopy (PFM) and X-Ray...article 1999
- document
-
Brocken, H.J.P. (author), Spiekman, M.E. (author), Pel, L. (author), Kopinga, K. (author), Larbi, J.A. (author), TNO Bouw (author)The water extraction out of mortar during brick laying was studied using nuclear magnetic resonance. The experiments show that using a fired-clay brick, the water is extracted out of the mortar within 3 minutes, whereas in the case of a sand-lime brick this takes about 10 minutes. Prewetting a fired-clay brick hardly affects the water extraction...article 1998
- document
-
Kleter, G.A. (author), Damen, J.J.M. (author), van den Kettenes-Bosch, J.J. (author), Bank, R.A. (author), te Koppele, J.M. (author), Veraart, J.R. (author), ten Cate, J.M. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)The aim was to identify suspect collagen cross-links in dentine, eluting close to known cross-links in ion-exchange HPLC. Bovine tooth roots as source of dentine were powdered, demineralised, reduced, and acid-hydrolysed. Cross-linking amino acids were isolated from the acid hydrolysate by size exclusion, adsorption, and sequential ion exchange...article 1998
- document
-
Centraal Instituut voor Voedingsonderzoek TNO (author), van Iersel, M.L.P.S. (author), van Lipzig, M.M.H. (author), Rietjens, I.M.C.M. (author), Vervoort, J. (author), van Bladeren, P.J. (author)Using 1H NMR two diastereoisomers of the ethacrynic acid glutathione conjugate (EASG) as well as ethacrynic acid (EA) could be distinguished and quantified individually. Chemically prepared EASG consists of equal amounts of both diastereoisomers. GSTP1-1 stereospecifically catalyzes formation of one of the diastereoisomers (A). The GSTP1-1...article 1998
- document
-
Koops, M. (author), Huijser, T. (author), Karthaus, W. (author), Prins Maurits Laboratorium TNO (author)Fibre armatures have been studied both dynamically and statically to gain insight in their electrothermal and mechanical behaviour. In the first part of this paper, the results of launch experiments with single and multi-segment copper and molybdenum fibre armatures integrated in carbon-fibre reinforced launch packages are discussed. The launch...article 1997
- document
-
Instituut voor Bouwmaterialen en Bouwconstructies TNO (author), Brocken, H.J.P. (author), Adant, O.C.G. (author), Pel, L. (author)The moisture transport in mortar and mortar joint often is an important parameter in degeneration of brick masonry and other block constructions. In this study, the influence of single additives on the moisture transport properties of mortar is investigated. Due to water extraction during brick laying, curing conditions of mortar in mortar joint...article 1997
- document
-
Medisch Biologisch Laboratorium TNO (author), Allaerts, W. (author), Jeucken, P.H.M. (author), Debets, R. (author), Hoefakker, S. (author), Claassen, E. (author), Drexhage, H.A. (author)The population of folliculo-stellate (FS) cells of the rat anterior pituitary has been shown to be ultrastructurally and immunohistochemically heterogeneous. Based on the overlap of ultrastructural characteristics, the localization in the anterior pituitary and the co-expression within the same cel of the S-100 protein (a marker for FS cells)...article 1997
- document
-
Rompelberg, C.J.M. (author), Vogels, J.T.W.E. (author), de Vogel, N. (author), Bruijntjes-Rozier, G.C.D.M. (author), Stenhuis, W.H. (author), Bogaards, J.J.P. (author), Verhagen, H. (author), TNO Voeding (author)1. In order to study the antigenotoxic potential of eugenol in humans, ten healthy non-smoking males ingested a daily amount of 150 mg eugenol or the placebo for seven consecutive days. After a washout period of one week, groups ingesting eugenol or the placebo were crossed and received the other treatment for seven consecutive days. 2. On days...article 1996
- document
-
Soffers, A.E.M.F. (author), Ploemen, J.H.T.M. (author), Moonen, M.J.H. (author), Wobbes, T. (author), van Ommen, B. (author), Vervoort, J. (author), van Bladeren, P.J. (author), Rietjens, I.M.C.M. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)Quantitative structure - activity relationships (QSAR's) are described for the rate of conjugation of a series of fluoronitrobenzenes with cytosolic as well as with two major alpha and mu class enzymes of rat and human liver, viz., glutathione S-transferases (GST) 1-1, 3-3, A1-1, and M1a-1a. For all purified enzymes studied, the natural...article 1996
- document
-
van Oostayen, J.A. (author), Bezemer, R.A. (author), Wasser, M.N.J.M. (author), Teirlinck, C.J.P.M. (author), Instituut voor Gezondheidstechniek TNO (author)Objective: To validate Doppler ultrasound and MRI mean velocity measurements in a test object in which mean velocities are known and can be chosen within a range of 10-100 cm/s in tubes of 4 and 8 mm. This validation was carried out to check the performance of a duplex Doppler ultrasound system that is used to determine volume blood flow in a...article 1996
- document
-
TNO Preventie en Gezondheid Gaubius Instituut TNO (author), Biessen, E.A.L. (author), Noorman, F. (author), van Teijlingen, M.E. (author), Kuiper, J. (author), Barrett-Bergshoeff, M.M. (author), Bijsterbosch, M.K. (author), Rijken, D.G. (author), van Berkel, T.J.G. (author)In search of synthetic high affinity ligands for the mannose receptor, we synthesized a series of lysine-based oligomannosides containing two (M2L) to six (M6L5) terminal alpha-D-mannose groups that are connected with the backbone by flexible elongated spacers (16 Å). The synthesized cluster mannosides were all able to displace binding of...article 1996
- document
-
Dirven, H.A.A.M. (author), Megens, L. (author), Oudshoorn, M.J. (author), Dingemanse, M.A. (author), van Ommen, B. (author), van Bladeren, P.J. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)Development of drug resistance against alkylating cytostatic drugs has been associated with higher intracellular concentrations of glutathione (GSH) and increased expression of glutathione S-transferase (GST) enzymes. Therefore, enhanced detoxification by the glutathione/glutathione S-transferase pathway has been proposed as a major factor in...article 1995
- document
-
Dirven, H.A.A.M. (author), Dictus, E.L.J.T. (author), Broeders, N.L.H.L. (author), van Ommen, B. (author), van Bladeren, P.J. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)Nonenzymatic and glutathione S-transferase (GST) catalyzed glutathione (GSH) conjugation has been postulated as a mechanism by which alkylating cytostatic drugs can be inactivated intracellularly. In this study, we describe studies on the glutathione-dependent biotransformation of thiotepa (tris(1-aziridinyl)phosphine sulfide), a trifunctional...article 1995
- document
-
Instituut CIVO-Toxicologie en Voeding TNO (author), den Besten, C. (author), van Bladeren, P.J. (author), Duizer, E. (author), Vervoort, J. (author), Rietjens, I.M.C.M. (author)In the present study the oxidative dehalogenation of a para-halogenated phenol was studied using pentafluorophenol and its non-para-halogenated analogue 2,3,5,6-tetrafluorophenol as model compounds. 19F NMR was used to characterize the metabolite patterns. In order to study the primary oxidation products of the microsomal cytochrome P450...article 1993
- document
-
Banta, H.D. (author)In this paper, computerized tomography scanning and magnetic resonance imaging scanning are discussed to illustrate some aspects of health care technology assessment in the field of diagnostic imaging. Technology assessment is defined as a comprehensive form of policy research that examines short- and long-term social consequences of the...article 1992
- document
-
Lokman, B.C. (author), van Santen, P. (author), Verdoes, J.C. (author), Kruse, J. (author), Leer, R.J. (author), Posno, M. (author), Pouwels, P.H. (author), Medisch Biologisch Laboratorium TNO (author)A cluster of three genes involved in D-xylose catabolism (viz. xylose genes) in Lactobacillus pentosus has been cloned in Escherichia coli and characterized by nucleotide sequence analysis. The deduced gene products show considerable sequence similarity to a repressor protein involved in the regulation of expression of xylose genes in Bacillus...article 1991
- document
-
van Nesselrooij, J.H.J. (author), Bruijntjes, J.P. (author), Garderen van-Hoetmer, A. (author), Tillapaugh-Fay, G.M. (author), Feron, V.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Estrogen-induced pituitary lesions in rats were studied in time-sequence experiments using magnetic resonance imaging (MRI), hormone determinations and light microscopy. The main purpose of the study was to evaluate the usefulness of MRI in comparison with conventional biochemical and histopathological methods for detecting the pituitary lesions...article 1991
- document
-
Benschop, H.P. (author), van den Berg, G.R. (author), van Hooidonk, C. (author), de Jong, L.P.A. (author), Kientz, C.E. (author), Berends, F. (author), Kepner, L.A. (author), Meeter, E. (author), Visser, R.P.L.S. (author), Medisch Biologisch Laboratorium TNO (author)Three new nonquaternary oximes have been evaluated with respect to their antidotal activities against organophosphate poisoning. The oximes 1,2,3-thiadiazole-5-carboxaldoxime (1, pKa = 7.6), 2-methyl-1,3,4-thiadiazole-5-carboxaldoxime (2, pKa = 8.4), and 3-methyl-1,2,4-thiadiazole-5-carboxaldoxime (3, pKa = 7.0) were prepared in yields of 56, 24...article 1979