- document
-
Bikker, F.J. (author), Kaman-van Zanten, W.E. (author), de Vries-van de Ruit, A.M.B.C. (author), Voskamp-Visser, I. (author), van Hooft, P.A.V. (author), Mars-Groenendijk, R.H. (author), de Visser, P.C. (author), Noort, D. (author), TNO Defensie en Veiligheid (author)Drosocin is a 19-mer, cationic antimicrobial peptide from Drosophila melanogaster. The aim of the study was to examine the antibacterial spectrum of unglycosylated drosocin analogues. Furthermore, the amino acid sequence of DnaK, drosocin's intracellular target, from susceptible species was aligned and studied for sequence homology. From this a...article 2006
- document
-
Verhoeckx, K.C.M. (author), Doornbos, R.P. (author), Witkamp, R.F. (author), van der Greef, J. (author), Rodenburg, R.J.T. (author), TNO Kwaliteit van Leven (author)Vascular endothelial growth factor (VEGF), oncostatin M (OSM), and granulocyte chemotactic protein-2 (GCP-2/CXCL6) are up-regulated in U937 macrophages and peripheral blood macrophages exposed to LPS, beta-adrenergic receptor (β2-AR) agonists (e.g. zilpaterol, and clenbuterol) and some other agents that induce intracellular cAMP (prostaglandin...article 2006
- document
-
Verhoeckx, K.C.M. (author), Gaspari, M. (author), Bijlsma, S. (author), van der Greef, J. (author), Witkamp, R.F. (author), Doornbos, R.P. (author), Rodenburg, R.J.T. (author), TNO Kwaliteit van Leven (author)Two-dimensional difference gel electrophoresis (DIGE) in combination with univariate (Student's t-test) and multivariate data analysis, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used to study the anti-inflammatory effects of the β2-adrenergic receptor (β2-AR) agonist zilpaterol. U937...article 2005
- document
-
Berbée, J.F.P. (author), Havekes, L.M. (author), Rensen, P.C.N. (author), TNO Kwaliteit van Leven (author)An increasing body of evidence demonstrates a close interplay between lipoprotein metabolism and sepsis. Sepsis results in an increase of plasma triglycerides within VLDL as a consequence of an enhanced hepatic VLDL production and/or inhibited peripheral and hepatic VLDL clearance. In contrast, sepsis decreases plasma cholesterol within LDL and...article 2005
- document
-
Verhoeckx, K.C.M. (author), Doornbos, R.P. (author), van der Greef, J. (author), Witkamp, R.F. (author), Rodenburg, R.J.T. (author), TNO Kwaliteit van Leven (author)In this study the anti-inflammatory properties of zilpaterol, a β2-adrenergic receptor (AR) agonist specifically developed as a growth promoter in cattle were investigated. Although zilpaterol has a different structure compared with the β2-AR agonists known to date, it was noted that it was able to bind to both the β2-AR (K i = 1.1 × 10-6) and...article 2005
- document
-
TNO Preventie en Gezondheid (author), Lund, S. (author), Porzgen, P. (author), Mortensen, A.L. (author), Hasseldam, H. (author), Bozyczko-Coyne, D. (author), Morath, S. (author), Hartung, T. (author), Bianchi, M. (author), Ghezzi, P. (author), Bsibsi, M. (author), Dijkstra, S. (author), Leist, M. (author)CEP-1347 is a potent inhibitor of the mixed lineage kinases (MLKs), a distinct family of mitogen-activated protein kinase kinase kinases (MAPKKK). It blocks the activation of the c-Jun/JNK apoptotic pathway in neurons exposed to various stressors and attenuates neurodegeneration in animal models of Parkinson's disease (PD). Microglial activation...article 2005
- document
-
Izeboud, C.A. (author), Hoebe, K.H.N. (author), Grootendorst, A.F. (author), Nijmeijer, S.M. (author), van Miert, A.S. (author), Witkamp, R.F. (author), Rodenburg, R.J.T. (author), TNO Voeding (author)Objective and Design: To investigate the effects of β2- adrenoceptor (β2-AR) stimulation on endotoxin-induced liver damage and systemic cytokine levels in rats. Subjects: Standard male Wistar rats. Treatment: A disease-model of lipolysaccharide (LPS)-induced acute systemic inflammation was used. The β2-selective AR agonist clenbuterol was...article 2004
- document
-
Hollestelle, S.C.G. (author), de Vries, M.R. (author), van Keulen, J.K. (author), Schoneveld, A.H. (author), Vink, A. (author), Strijder, C.F. (author), van Middelaar, B.J. (author), Pasterkamp, G. (author), Q́uax, P.H.A. (author), de Kleijn, D.P.V. (author), TNO Preventie en Gezondheid (author)Background-Toll-like receptor 4 (Tlr4) is the receptor for exogenous lipopolysaccharides (LPS). Expression of endogenous Tlr4 ligands, heat shock protein 60 (Hsp60) and extra domain A of fibronectin, has been observed in arthritic and oncological specimens in which matrix turnover is an important feature. In atherosclerosis, outward remodeling...article 2004
- document
-
Verhoeckx, K.C.M. (author), Bijlsma, S. (author), Jespersen, S. (author), Ramaker, R. (author), Verheij, E.R. (author), Witkamp, R.F. (author), van der Greef, J. (author), Rodenburg, R.J.T. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)The discovery of new anti-inflammatory drugs is often based on an interaction with a specific target, although other pathways often play a primary or secondary role. Anti-inflammatory drugs can be categorized into classes, based on their mechanism of action. In this article we investigate the possibility to characterize novel anti-inflammatory...article 2004
- document
-
Killestein, J. (author), Hoogervorst, E.L.J. (author), Reif, M. (author), Blauw, B. (author), Smits, M. (author), Uitdehaag, B.M.J. (author), Nagelkerken, L. (author), Polman, C.H. (author), TNO Preventie en Gezondheid (author)Cannabinoids can modulate the function of immune cells. We here present the first human in vivo study measuring immune function in 16 MS patients treated with oral cannabinoids. A modest increase of TNF-α in LPS-stimulated whole blood was found during cannabis plant-extract treatment (p=0.037), with no change in other cytokines. In the subgroup...article 2003
- document
-
Visser, J. (author), Graffelman, W. (author), Blauw, B. (author), Haspels, I. (author), Lentjes, E. (author), de Kloet, R. (author), Nagelkerken, L. (author), TNO Preventie en Gezondheid (author)Several causes have been held responsible for the chronic fatigue syndrome (CFS), including an altered hypothalamus-pituitary-adrenal gland (HPA)-axis activity, viral infections and a reduced Th1 activity. Therefore, it was investigated whether the regulation of IL-10 is different in CFS. LPS-induced cytokine secretion in whole blood cultures...article 2001
- document
-
Izeboud, C.A. (author), Vermeulen, R.M. (author), Zwart, A. (author), Vos, H.P. (author), van Miert, A.S.J.P.A.M. (author), Witkamp, R.F. (author)Previous research has shown that β-adrenoceptor (β-AR) agonists have potent anti-inflammatory capabilities, e.g. represented by suppression of release of the proinflammatory cytokines. Aim of this research was to determine whether the effects of β-agonists on LPS-induced TNFα and IL-10 release are influenced by their different stereochemistry....article 2000
- document
-
van Baar, B.L.M. (author), Research Group Analytical and Toxic Explosive Substances, TNO Prins Maurits Laboratory, P.O. Box, Rijswijk, Netherlands (author)Chemical analysis for the characterisation of micro-organisms is rapidly evolving, after the recent advent of new ionisation methods in mass spectrometry (MS): electrospray (ES) and matrix-assisted laser desorption/ionisation (MALDI). These methods allow quick characterisation of micro-organisms, either directly or after minimum sample...article 2000
- document
-
Izeboud, C.A. (author), Monshouwer, M. (author), Witkamp, R.F. (author), van Miert, A.S.J. (author), TNO Voeding (author)During infection and inflammation drug disposition and hepatic metabolism are markedly affected in mammals. Pro-inflammatory mediators play an important role in the suppression of (cytochrome-P450-mediated) drug metabolism. Inflammatory mediators like cytokines, nitric oxide (NO), reactive oxygen species (ROS) and eicosanoids are released by...article 2000
- document
- TNO Pharma (author), Hoebe, K.H.N. (author), Monshouwer, M. (author), Witkamp, R.F. (author), Fink-Gremmels, J. (author), van Miert, A.S.J.P.A.M. (author) article 2000
- document
-
Izeboud, C.A. (author), Monshouwer, M. (author), van Miert, A.S.J.P.A.M. (author), Witkamp, R.F. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Objective and Design: To investigate the suppressive effects of the β-agonist clenbuterol on the release of TNF-α and IL-6 in a lipopolysaccharide (LPS)-model of inflammation, both in vitro and in vivo. Material and Subjects: Human U-937 cell line (monocyte-derived macrophages), and male Wistar rats (200-250 g). Treatment: U-937 macrophages were...article 1999
- document
-
Participation of β-adrenergic receptors on macrophages in modulation of LPS-induced cytokine releaseIzeboud, C.A. (author), Mocking, J.A.J. (author), Monshouwer, M. (author), van Miert, A.S.J.P.A.M. (author), Witkamp, R.F. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)For several years it is known that β-adrenergic receptor agonists have anti-inflammatory effects. However, little is known about the role of β- adrenergic receptors on macrophages in the modulation of cytokine production by β-agonists during inflammation. In this study, the presence of β- receptors on PMA-differentiated U937 human macrophages,...article 1999
- document
-
van Rozendaal, B.A.W.M. (author), van de Lest, C.H.A. (author), van Eijk, M. (author), van Golde, L.M.G. (author), Voorhout, W.F. (author), van Helden, H.P.M. (author), Haagsman, H.P. (author)Collectins are carbohydrate binding proteins that are implicated in innate host defense. The lung collectins, surfactant proteins A and D (SP-A and SP-D), bind a variety of pathogens in vitro and influence phagocytosis by alveolar macrophages. In this report we show that SP-D binds endotoxin (lipopolysaccharide, LPS) in vivo in a rat model of...article 1999
- document
-
Visser, J. (author), van Boxel-Dezaire, A. (author), Methorst, D. (author), Brunt, T. (author), de Kloet, E.R. (author), Nagelkerken, L. (author), TNO Preventie en Gezondheid (author)Antigen-presenting cells are thought to modulate the development of Th1 and Th2 cells by the secretion of interleukin-10 (IL-10) and IL-12. Because glucocorticoids (GC) favor the development of Th2 responses, we determined whether dexamethasone (DEX) and hydrocortisone (HC) have differential effects on lipopolysaccharide-induced IL-10 and IL-12...article 1998
- document
-
Prins Maurits Laboratorium TNO (author), van Helden, H.P.M. (author), Kuijpers, W.C. (author), Langerwerf, P.E.J. (author), Langen, R.C.J. (author), Haagsman, H.P. (author), Bruijnzeel, P.L.B. (author)Curosurf, a natural lung surfactant, is considered a potential candidate for improving the treatment of acute respiratory distress syndrome (ARDS). To investigate this in a rat model of early-stage ARDS, Curosurf (62.5, 125 or 250 mg·kg-1) was administered by intratracheal bolus at 10 or 24 h following an intratracheal lipopolysaccharide (LPS; 1...article 1998