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Mione, M.A. (author), Vandalon, V. (author), Mameli, A. (author), Kessels, W.M.M. (author), Roozeboom, F. (author)An atmospheric-pressure plasma-enhanced spatial atomic layer deposition (PE-s-ALD) process for SiO2 using bisdiethylaminosilane (BDEAS, SiH2[NEt2]2) and O2 plasma is reported along with an investigation of its underlying growth mechanism. Within the temperature range of 100−250 °C, the process demonstrates self-limiting growth with a growth per...article 2021
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- Vink, J.P.M. (author), Comans, R.N.J. (author), Dijkstra, J.J. (author), Lamers, L.P.M. (author) article 2020
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de Almeida, J.S.F.D. (author), Cuya Guizado, T.R. (author), Guimarães, A.P. (author), Ramalho, T.C. (author), Gonçalves, A.S. (author), de Koning, M.C. (author), França, T.C.C. (author)In the present work, we performed docking and molecular dynamics simulations studies on two groups of long-tailored oximes designed as peripheral site binders of acetylcholinesterase (AChE) and potential penetrators on the blood brain barrier. Our studies permitted to determine how the tails anchor in the peripheral site of sarin-inhibited human...article 2016
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Lai, W.-Y. (author), Balfour, M.N. (author), Levell, J.W. (author), Bansal, A.K. (author), Burn, P.L. (author), Lo, S.-C. (author), Samuel, I.D.W. (author)Phosphorescent poly(dendrimers) with a norbornene-derived backbone have been synthesized using ring-opening metathesis polymerization with the Grubbs III catalyst. The dendrimers are comprised of a heteroleptic iridium(III) complex core with two 2-phenylpyridyl ligands and a phenyltriazolyl ligand, biphenyl-based dendrons, and 2-ethylhexyloxy...article 2012
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van der Horst, E. (author), Peironcely, J.E. (author), van Westen, G.J.P. (author), van den Hoven, O.O. (author), Galloway, W.R.J.D. (author), Spring, D.R. (author), Wegner, J.K. (author), van Vlijmen, H.W.T. (author), Ijzerman, A.P. (author), Overington, J.P. (author), Bender, A. (author)Chemogenomic approaches, which link ligand chemistry to bioactivity against targets (and, by extension, to phenotypes) are becoming more and more important due to the increasing number of bioactivity data available both in proprietary databases as well as in the public domain. In this article we review chemogenomics approaches applied in four...article 2011
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End, C. (author), Bikker, F.J. (author), Renner, M. (author), Bergmann, G. (author), Lyer, S. (author), Blaich, S. (author), Hudler, M. (author), Helmke, B. (author), Gassler, N. (author), Autschbach, F. (author), Ligtenberg, A.J.M. (author), Benner, A. (author), Holmskov, U. (author), Schirmacher, P. (author), Nieuw Amerongen, A.V. (author), Rosenstiel, P. (author), Sina, C. (author), Franke, A. (author), Hafner, M. (author), Kioschis, P. (author), Schreiber, S. (author), Poustka, A. (author), Mollenhauer, J. (author)Deleted in malignant brain tumors 1 (DMBT1) is a secreted glycoprotein displaying a broad bacterial-binding spectrum. Recent functional and genetic studies linked DMBT1 to the suppression of LPS-induced TLR4-mediated NF-kappaB activation and to the pathogenesis of Crohn's disease. Here, we aimed at unraveling the molecular basis of its function...article 2009
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Boele, L.C.L. (author), Bajramovic, J.J. (author), de Vries, A.M.M.B.C. (author), Voskamp-Visser, I.A.I. (author), Kaman, W.E. (author), van der Kleij, D. (author), TNO Defensie en Veiligheid (author)Activation of pattern recognition receptors such as Toll-like receptors (TLRs) by pathogens leads to activation and maturation of dendritic cells (DC), which orchestrate the development of the adaptive immune response. To create an overview of the effects of a broad range of pathogenic bacteria, their capacity to activate TLRs and to affect DC...article 2009
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Vink, J.P.M. (author), TNO Bouw en Ondergrond (author)The speciation of heavy metals was measured over a variety of natural and undisturbed water/sediment interfaces. Simultaneously, two benthic species (oligochaete Limnodrilus spp. and the midge Chironomus riparius) were exposed to these sediments. Under occurring redox conditions, free ion activities of trace metals Cd, Cu, Ni, Pb, and Zn were...article 2009
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TNO Defensie en Veiligheid (author), Molhoek, E.M. (author), den Hertog, A.L. (author), de Vries, A.M.B.C. (author), Nazmi, K. (author), Veerman, E.C.I. (author), Hartgers, F.C. (author), Yazdanbakhsh, M. (author), Bikker, F.J. (author), van der Kleij, D. (author)Cathelicidins are effector molecules of the innate host defense system that establish an antimicrobial barrier at epithelial interfaces. The human cathelicidin LL-37, in addition to its antimicrobial activity, also exhibits immunomodulatory effects, such as inhibition of pro-inflammatory responses to bacterial LPS in human monocytic cells. In...article 2009
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Retra, K. (author), van Riet, E. (author), Adegnika, A.A. (author), Everts, B. (author), van Geest, S. (author), Kremsner, P.G. (author), van Hellemond, J.J. (author), van der Kleij, D. (author), Tielens, A.G.M. (author), Yazdanbakhsh, M. (author), TNO Defensie en Veiligheid (author)Schistosomes carry lipid moieties that interact with the immune system. To understand the consequence of interactions in terms of polarizing the cytokine profiles, the effect of two Toll-like receptor-2 (TLR2) activating schistosomal lipid fractions was studied on whole blood from Gabonese children living in a schistosomiasis endemic area. One...article 2008
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TNO Kwaliteit van Leven (author), Hu, L. (author), van der Hoogt, C.C. (author), Espirito Santo, S.M.S. (author), Out, R. (author), Kypreos, K.E. (author), van Vlijmen, B.J.M. (author), van Berkel, T.J.C. (author), Romijn, J.A. (author), Havekes, L.M. (author), van Dijk, K.W. (author), Rensen, P.C.N. (author)LPL activity plays an important role in preceding the VLDL remnant clearance via the three major apolipoprotein E (apoE)-recognizing receptors: the LDL receptor (LDLr), LDL receptor-related protein (LRP), and VLDL receptor (VLDLr). The aim of this study was to determine whether LPL activity is also important for VLDL remnant clearance...article 2008
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Kraaijeveld, A.O. (author), de Jager, S.C.A. (author), de Jager, W.J. (author), Prakken, B.J. (author), McColl, S.R. (author), Haspels, I. (author), Putter, H. (author), van Berkel, T.J.C. (author), Nagelkerken, L. (author), Jukema, J.W. (author), Biessen, E.A.L. (author), TNO Kwaliteit van Leven (author)BACKGROUND - Chemokines play an important role in atherogenesis and in ischemic injury and repair; however, prospective data on individual chemokines in unstable angina pectoris (UAP) are scarce. Therefore, we assessed chemokine patterns in a prospective cohort of patients with UAP. METHODS AND RESULTS - Plasma samples of 54 patients with...article 2007
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Oude Nijhuis, M.M. (author), van Keulen, J.K. (author), Pasterkamp, G. (author), Quax, P.H. (author), de Kleijn, D.P.V. (author), TNO Kwaliteit van Leven (author)Innate immunity is the first line of defence against invading micro-organisms. The family of Toll-like receptors (TLRs) recognizes pathogen-associated molecular patterns (PAMPs) that are carried by the invading micro-organisms. Infectious pathogens have been implicated to play an important role in atherosclerosis. Nowadays, evidence is...article 2007
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van Wijk, F. (author), Nierkens, S. (author), de Jong, W. (author), Wehrens, E.J.M. (author), Boon, L. (author), van Kooten, P. (author), Knippels, L.M.J. (author), Pieters, R. (author), TNO Kwaliteit van Leven (author)Dendritic cells are believed to play an essential role in regulating the balance between immunogenic and tolerogenic responses to mucosal Ags by controlling T cell differentiation and activation via costimulatory and coinhibitory signals. The CD28/CTLA-4-CD80/CD86 signaling pathway appears to be one of the most important regulators of T cell...article 2007
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Bernhagen, J. (author), Krohn, R. (author), Lue, H. (author), Gregory, J.L. (author), Zernecke, A. (author), Koenen, R.R. (author), Dewor, M. (author), Georgiev, I. (author), Schober, A. (author), Leng, L. (author), Kooistra, T. (author), Fingerle-Rowson, G. (author), Ghezzi, P. (author), Kleemann, R. (author), McColl, S.R. (author), Bucala, R. (author), Hickey, M.J. (author), Weber, C. (author), TNO Kwaliteit van Leven (author)The cytokine macrophage migration inhibitory factor (MIF) plays a critical role in inflammatory diseases and atherogenesis. We identify the chemokine receptors CXCR2 and CXCR4 as functional receptors for MIF. MIF triggered G αi- and integrin-dependent arrest and chemotaxis of monocytes and T cells, rapid integrin activation and calcium influx...article 2007
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van Wijk, F. (author), Knippels, L. (author), TNO Kwaliteit van Leven (author)Immediately after birth the mucosa of the gastrointestinal tract, which represents the greatest body surface area exposed to the outside environment, is confronted with a large variety of foreign antigens. The immune system of the intestine now has to meet the task of discriminating between pathogens and harmless antigens, such as food proteins...article 2007
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Steenvoorden, M.M.C. (author), Toes, R.E.M. (author), Ronday, H.K. (author), Huizinga, T.W.J. (author), de Groot, J. (author), TNO Kwaliteit van Leven (author)Ligands for the receptor for advanced glycation endproducts (RAGE) are increased in RA synovial fluid (SF), serum and synovium. Since RAGE is present on fibroblast-like synoviocytes (FLS), the present study investigates whether the RAGE ligands HMGB-1 and AGEs are able to stimulate the characteristic, pathological invasive behaviour of these...article 2007
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Lopatinskaya, L. (author), Zwemmer, J. (author), Uitdehaag, B. (author), Lucas, K. (author), Polman, C. (author), Nagelkerken, L. (author), TNO Kwaliteit van Leven (author)TNF-α, IL-12p35, IL-12p40, IL-4, IL-10, TGF-β1, CCR3, CXCR3, CCR5, Fas and FasL mRNA levels in PBMC of 25 multiple sclerosis (MS) patients were quantified at baseline by real-time PCR according to a post-hoc study design. The baseline values of the different markers were analysed with respect to their correlation with the increase in disability...article 2006
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Mandelin, J. (author), Hukkanen, M. (author), Li, T.F. (author), Korhonen, M. (author), Liljeström, M. (author), Sillat, T. (author), Hanemaaijer, R. (author), Salo, J. (author), Santavirta, S. (author), Konttinen, Y.T. (author), TNO Kwaliteit van Leven (author)Healthy bone is a rigid yet living tissue that undergoes continuous remodeling. Osteoclasts resorb bone in the remodeling cycle. They secrete H+-ions and proteinases to dissolve bone mineral and degrade organic bone matrix, respectively. One of the main collagenolytic proteinase in osteoclasts is cathepsin K, a member of papain family cysteine...article 2006
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Burger-Kentischer, A. (author), Göbel, H. (author), Kleemann, R. (author), Zernecke, A. (author), Bucala, R. (author), Leng, L. (author), Finkelmeier, D. (author), Geiger, G. (author), Schaefer, H.E. (author), Schober, A. (author), Weber, C. (author), Brunner, H. (author), Rütten, H. (author), Ihling, C. (author), Bernhagen, J. (author), TNO Kwaliteit van Leven (author)Atherosclerosis is an inflammatory response of the arterial wall to "injury", which is prominently driven by cytokines. The inflammatory mediator macrophage migration inhibitory factor (MIF) is a unique cytokine that was recently associated with atherogenesis. Here, we have investigated whether MIF has a role in spontaneous atherosclerosis by...article 2006