Searched for: subject%3A%22Isolation%255C%2Band%255C%2Bpurification%22
(1 - 19 of 19)
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Zhao, Y. (author), Kumar, M. (author), Caspers, M.P.M. (author), Nierop Groot, M.N. (author), van der Vossen, J.M.B.M. (author), Abee, T. (author)Thermophilic bacilli such as Anoxybacillus and Geobacillus are important contaminants in dairy powder products. Remarkably, one of the common contaminants, Geobacillus thermoglucosidans, showed poor growth in skim milk, whereas significant growth of G. thermoglucosidans was observed in the presence of an Anoxybacillus flavithermus dairy isolate....article 2018
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- de Marchi, T. (author), Braakman, R.B.H. (author), Stingl, C. (author), van Duijn, M.M. (author), Smid, M. (author), Foekens, J.A. (author), Luider, T.M. (author), Martens, J.W.M. (author), Umar, A. (author) article 2016
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TNO Kwaliteit van Leven (author), Gautam, R. (author), Borgdorff, H. (author), Jespers, V. (author), Francis, S.C. (author), Verhelst, R. (author), Mwaura, M. (author), Delany-Moretlwe, S. (author), Ndayisaba, G. (author), Kyongo, J.K. (author), Hardy, L. (author), Menten, HJ. (author), Crucitti, T. (author), Tsivtsivadze, E. (author), Schuren, F. (author), van de Wijgert, J.H.R.M. (author), Vaginal biomarkers Study Group, (author)Background: Sociodemographic, behavioral and clinical correlates of the vaginal microbiome (VMB) as characterized by molecular methods have not been adequately studied. VMB dominated by bacteria other than lactobacilli may cause inflammation, which may facilitate HIV acquisition and other adverse reproductive health outcomes. Methods: We...article 2015
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Jónasdóttir, H.S. (author), Ioan-Facsinay, A. (author), Kwekkeboom, J. (author), Brouwers, H. (author), Zuurmond, A.M. (author), Toes, R. (author), Deelder, A.M. (author), Giera, M. (author)Here we present an advanced platform for the analysis of specialized pro-resolving mediators (SPM), including a set of pro-inflammatory lipids and respective biochemical pathway markers. The platform is characterized by a largely simplified sample preparation protocol, employing methanol protein-precipitation instead of solid phase extraction....article 2014
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Visser, A. (author), Schaap, J.D. (author), Broers, H.P. (author), Bierkens, M.F.P. (author), TNO Bouw en Ondergrond (author)The production of N2 gas by denitrification may lead to the appearance of a gas phase below the water table prohibiting the conservative transport of tracer gases required for groundwater dating. We used a two-phase flow and transport model (STOMP) to study the reliability of 3H/3He, CFCs and SF6 as groundwater age tracers under agricultural...article 2009
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Paauw, A. (author), Caspers, M.P.M. (author), Leverstein-van Hall, M.A. (author), Schuren, F.H.J. (author), Montijn, R.C. (author), Verhoef, J. (author), Fluit, A.C. (author), TNO Kwaliteit van Leven (author)Bacterial strains differ in their ability to cause hospital outbreaks. Using comparative genomic hybridization, Enterobacter cloacae complex isolates were studied to identify genetic markers specific for Enterobacter cloacae complex outbreak strains. No outbreak-specific genes were found that were common in all investigated outbreak strains....article 2009
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Wang, Y. (author), Xiao, Y. (author), Zhang, Y. (author), Chai, C. (author), Wei, G. (author), Wei, X. (author), Xu, H. (author), Wang, M. (author), Ouwerkerk, P.B.F. (author), Zhu, Z. (author), TNO Kwaliteit van Leven (author)Monosaccharides transporters play important roles in assimilate supply for sink tissue development. In this study, a new monosaccharide transporter gene OsMST6 was identified from rice (Oryza sativa L.). The predicted OsMST6 protein shows typical features of sugar transporters and shares 79.6% identity with the rice monosaccharide transporter...article 2008
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Goosen, C. (author), Yuan, X.L. (author), van Munster, J.M. (author), Ram, A.F.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)A novel subfamily of putative intracellular invertase enzymes (glycoside hydrolase family 32) has previously been identified in fungal genomes. Here, we report phylogenetic, molecular, and biochemical characteristics of SucB, one of two novel intracellular invertases identified in Aspergillus niger. The sucB gene was expressed in Escherichia...article 2007
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van Boxtel, E.L. (author), van Koningsveld, G.A. (author), Koppelman, S.J. (author), van den Broek, L.A.M. (author), Voragen, A.G.J. (author), Gruppen, H. (author), TNO Kwaliteit van Leven (author)A new, fast, large-scale purification method for Ber e 1, the major allergen from Brazil nuts, using expanded bed adsorption (EBA) chromatography, is presented. Using EBA, crude extracts can be applied to a fluidized column, which allows the unhindered passage of particulate impurities, thereby avoiding time-consuming centrifugation or...article 2006
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Hooijschuur, E.W.J. (author), Kientz, C.E. (author), Brinkman, U.A.T. (author)Today, the determination of chemical warfare agents (CWAs) is an important area of application in analytical chemistry. Chromatographic, capillary electrophoretic and mass spectrometric techniques are primarily used for the identification and quantification of a broad field of classical CWAs in environmental samples and neutralization masses,...article 2002
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TNO Voeding (author), Fischer, M. (author), Gruppen, H. (author), Piersma, S.R. (author), Kofod, L.V. (author), Schols, H.A. (author), Voragen, A.G.J. (author)With the purpose of analyzing the size and composition of enzyme-unextractable proteins in differently heat-treated soybean meals, a selection of extractants was screened for their ability to extract these proteins from enzyme-unextractable residues. The largest effects were obtained with urea, urea plus β-mercaptoethanol, and dilute alkali; the...article 2002
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Gucek, M. (author), Gaspari, M. (author), Walhagen, K. (author), Vreeken, R.J. (author), Verheij, E.R. (author), van der Greef, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The successful coupling of capillary electrochromatography (CEC) to an ion trap mass spectrometer via a nanoelectrospray interface (nESI) is described. Using a conductively coated tip butted to the end of a CEC column, it was possible to obtain a stable spray without any sheath liquid being employed. Selected small peptides were separated with...article 2000
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Pots, A.M. (author), Gruppen, H. (author), Hessing, M. (author), van Boekel, M.A.J.S. (author), Voragen, A.G.J. (author)Patatin has, so far, been considered a homogeneous group of proteins. A comparison of the isoforms in terms of structural properties or stability has not been reported. A method to obtain various isoform fractions as well as a comparison of the physicochemical properties of these pools is presented. Patatin could be separated in four isoform...article 1999
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Steenwinkel, M.J.S.T. (author), Roggeband, R. (author), van Delft, J.H.M. (author), Baan, R.A. (author)This contribution describes methodological modifications and improvements that may contribute to inter-assay reproducibility and more accurate adduct quantification for 32P-postlabelling. Firstly, an anion-exchange chromatography procedure was developed to determine the amount of DNA used per assay and to check its purity, in particular to...bookPart 1993
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Hensgens, L.A.M. (author), Fornerod, M.W.J. (author), Rueb, S. (author), Winkler, A.A. (author), van der Veen, S. (author), Schilperoort, R.A. (author), Centrum voor Plantenfysiologisch Onderzoek (author)Transcriptional and translational fusions between the reading frame of the β-D-glucuronidase gene (gusA) and the 2′ as well as the 1′ promoter of mannopine synthase (mas), a TR locus of Agrobacterium tumefaciens, were made. The expression of these constructs was studied in the transgenic F1 offspring of independent tobacco transformants at the...article 1992
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Vaandrager, G.J. (author), Nederlands Instituut voor Praeventieve Gezondheidszorg TNO (author)Chemicals/CAS: Autoantibodies; islet cell antibody; Mumps Vaccinearticle 1986
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Centraal Instituut voor Voedingsonderzoek TNO (author), de Waart, J. (author)Chemicals/CAS: 3 nitropropionic acid, 504-88-1; aflatoxin B1, 1162-65-8; aflatoxin B2, 7220-81-7; aflatoxin G1, 1165-39-5; aflatoxin G2, 7241-98-7; aflatoxin, 1402-68-2; diacetoxyscirpenol, 2270-40-8; fumagillin, 23110-15-8; gliotoxin, 37273-88-4, 67-99-2; kojic acid, 501-30-4; luteoskyrin, 21884-44-6; ochratoxin, 303-47-9, 37203-43-3;...article 1973
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- Offers, S. (author), Bentvelzen, P. (author), Radiobiologisch Instituut TNO (author) article 1971
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Centraal Instituut voor Voedingsonderzoek TNO (author), Narayan, K.G. (author), Guinée, P.A.M. (author), Mossel, D.A.A. (author)A comparative examination of Patho-Tec reagent-impregnated paper strips and the corresponding classical biochemical tests for the identification of Enterobacteriaceae was made. Patho-Tec papers appeared to be reliable for the cytochrome-oxidase and phenylalanine-deaminase tests. The urease test papers gave false negative reactions in Citrobacter...article 1967
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