Searched for: subject%3A%22Inulosucrase%22
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TNO Kwaliteit van Leven TNO Voeding (author), Ozimek, L.K. (author), Kralj, S. (author), Kaper, T. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)
Bacterial fructansucrase enzymes belong to glycoside hydrolase family 68 and catalyze transglycosylation reactions with sucrose, resulting in the synthesis of fructooligosaccharides and/or a fructan polymer. Significant differences in fructansucrase enzyme product specificities can be observed, i.e. in the type of polymer (levan or inulin)...
article 2006
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TNO Kwaliteit van Leven TNO Voeding (author), Ozimek, L.K. (author), Euverink, G.J.W. (author), Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)
Bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (GH68) are not known to require a metal cofactor. Here, we show that Ca2+ ions play an important structural role in the Lactobacillus reuteri 121 levansucrase (Lev) and inulosucrase (Inu) enzymes. Analysis of the Bacillus subtilis Lev 3D structure [Meng, G. and...
article 2005
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Ozimek, L.K. (author), van Hijum, S.A.F.T. (author), van Koningsveld, G.A. (author), van der Maarel, M.J.E.C. (author), van Geel-Schutten, G.H. (author), Dijkhuizen, L. (author)
Bacterial fructosyltransferases (FTFs) are retaining-type glycosidases that belong to family 68 of glycoside hydrolases. Recently, the high-resolution 3D structure of the Bacillus subtilis levansucrase has been solved [Meng, G. and Futterer, K., Nat. Struct. Biol. 10 (2003) 935-941]. Based on this structure, the catalytic nucleophile, general...
article 2004
document
TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), van Hijum, S.A.F.T. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)
Inulosucrases catalyze transfer of a fructose moiety from sucrose to a water molecule (hydrolysis) or to an acceptor molecule (transferase), yielding inulin. Bacterial inulin production is rare and a biochemical analysis of inulosucrase enzymes has not been reported. Here we report biochemical characteristics of a purified recombinant...
article 2003
Searched for: subject%3A%22Inulosucrase%22
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