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Erpelinck, E. (author), Meijers, T. (author), Ariga, K. (author)The TNO InTESTine™ system is developed to study the absorption and translocation of pharmaceutical, biological and nutritional compounds across the intestinal wall in a physiologically relevant model. In this medium-throughput system, fresh ex vivo intestinal tissue from human or animal origin is mounted into a two compartment model with an...other 2019
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Verhoeckx, K. (author), Bogh, K.L. (author), Dupont, D. (author), Egger, L. (author), Gadermaier, G. (author), Larre, C. (author), Mackie, A. (author), Menard, O. (author), Adel-Patient, K. (author), Picariello, G. (author), Portmann, R. (author), Smit, J. (author), Turner, P. (author), Untersmayr, E. (author), Epstein, M.M. (author)The current allergenicity assessment of novel proteins is based on the EFSA GMO guidance. Recently, EFSA launched a new guidance document on allergenicity assessment of GM plants (2017). This document describes, amongst other topics, the new scientific and regulatory developments on in vitro protein digestibility tests. The EFSA GMO Panel stated...article 2019
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Bogerd, C.P. (author), Velt, K.B. (author), Annaheim, S. (author), Bongers, C.C.W.G. (author), Eijsvogels, T.M.H. (author), Daanen, H.A.M. (author)OBJECTIVE: The discomfort caused by rectal probes and esophageal probes for the estimation of body core temperature has triggered the development of gastrointestinal (GI) capsules that are easily accepted by athletes and workers due to their non-invasive characteristics. We compare two new GI capsule devices with rectal temperature during cycle...article 2018
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Fieten, K.B. (author), Totté, J.E.E. (author), Levin, E. (author), Reyman, M. (author), Meijer, Y. (author), Schuren, F.H.J. (author), Pasmans, S.G.M.A. (author), Pasmans, S.G.M.A. (author)Background: Exposure to microbes may be important in the development of atopic disease. Atopic diseases have been associated with specific characteristics of the intestinal microbiome. The link between intestinal microbiota and food allergy has rarely been studied, and the gold standard for diagnosing food allergy (double-blind placebo...article 2018
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Botschuijver, S. (author), Welting, O. (author), Levin, E. (author), Maria-Ferreira, D. (author), Koch, E. (author), Montijn, R.C. (author), Seppen, J. (author), Hakvoort, T.B.M. (author), Schuren, F.H.J. (author), de Jonge, W.J. (author), van den Wijngaard, R.M. (author)Background: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder associated with altered gastrointestinal microflora and increased nociception to colonic distension. This visceral hypersensitivity can be reversed in our rat maternal separation model by fungicides. Menthacarin® is a proprietary combination of essential oils from...article 2018
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Botschuijver, S. (author), Roeselers, G. (author), Levin, E. (author), Jonkers, D.M. (author), Welting, O. (author), Heinsbroek, S.E.M. (author), de Weerd, H.H. (author), Boekhout, T. (author), Fornai, M. (author), Masclee, A.A. (author), Schuren, F.H.J. (author), de Jonge, W.J. (author), Seppen, J. (author), van den Wijngaard, R.M. (author)Background & Aims Visceral hypersensitivity is one feature of irritable bowel syndrome (IBS). Bacterial dysbiosis might be involved in the activation of nociceptive sensory pathways, but there have been few studies of the role of the mycobiome (the fungal microbiome) in the development of IBS. We analyzed intestinal mycobiomes of patients with...article 2017
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Aguirre, M. (author), Venema, K. (author)There is an elevated incidence of cases of obesity worldwide. Therefore, the development of strategies to tackle this condition is of vital importance. This review focuses on the necessity of optimising in vitro systems to model human colonic fermentation in obese subjects. This may allow to increase the resolution and the physiological...article 2017
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Li, M. (author), de Graaf, I.A.M. (author), van de Steeg, E. (author), de Jager, M.H. (author), Groothuis, G.M.M. (author)Intestinal P-gp and CYP3A4 work coordinately to reduce the intracellular concentration of drugs, and drug-drug interactions (DDIs) based on this interplay are of clinical importance and require pre-clinical investigation. Using precision-cut intestinal slices (PCIS) of human jejunum, ileum and colon, we investigated the P-gp/CYP3A4 interplay and...article 2017
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Wopereis, S. (author), Stroeve, J.H.M. (author), Stafleu, A. (author), Bakker, G.C.M. (author), Burggraaf, J. (author), van Erk, M.J. (author), Pellis, L. (author), Boessen, R. (author), Kardinaal, A.A.F. (author), van Ommen, B. (author)Background: A key feature of metabolic health is the ability to adapt upon dietary perturbations. Recently, it was shown that metabolic challenge tests in combination with the new generation biomarkers allow the simultaneous quantification of major metabolic health processes. Currently, applied challenge tests are largely non-standardized. A...article 2017
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Mariman, R. (author), Reefman, E. (author), Tielen, F. (author), Persoon-Deen, C. (author), van de Mark, K. (author), Worms, N. (author), Koning, F. (author), Nagelkerken, L. (author)Genetic predisposition and environmental factors, including the gut microbiota, have been suggested as major factors in the development and progression of atopic dermatitis. Hyperlipidemic human APOC1+/+ transgenic mice display many features of human atopic dermatitis, such as scaling, lichenification, excoriations, and pruritus, along with a...article 2016
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Erpelinck, E. (author)TNO’s intestinal screening model (TNO i-screen) helps to quickly identify pharmacological compounds that are metabolized by intestinal microbiota. For pharmaceutical companies, searching for novel pharmaceuticals is a complex and time-consuming process. When a novel drug has been selected, extensive in vitro and clinical studies are required to...other 2016
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Ripken, D. (author), van der Wielen, N. (author), Wortelboer, H.M. (author), Meijerink, J. (author), Witkamp, R.F. (author), Hendriks, H.F.J. (author)Glucagon like peptide-1 (GLP-1) and serotonin are both involved in food intake regulation. GLP-1 release is stimulated upon nutrient interaction with G-protein coupled receptors by enteroendocrine cells (EEC), whereas serotonin is released from enterochromaffin cells (ECC). The central hypothesis for the current study was that nutrient-induced...article 2016
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Williams, F.M. (author), Rothe, H. (author), Barrett, G. (author), Chiodini, A. (author), Whyte, J. (author), Cronin, M.T.D. (author), Monteiro-Riviere, N.A. (author), Plautz, J. (author), Roper, C. (author), Westerhout, J. (author), Yang, C. (author), Guy, R.H. (author)Threshold of Toxicological Concern (TTC) aids assessment of human health risks from exposure to low levels of chemicals when toxicity data are limited. The objective here was to explore the potential refinement of exposure for applying the oral TTC to chemicals found in cosmetic products, for which there are limited dermal absorption data. A...article 2016
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Ripken, D. (author), Hendriks, H.F.J. (author)This chapter describes the use of the porcine ex vivo intestinal segment model. This includes the advantages and disadvantages of the segment model and a detailed description of the isolation and culture as well as the applications of the porcine ex vivo intestinal segment model in practice. Compared to the Ussing chamber (Chap. 24) the porcine...bookPart 2015
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Ripken, D. (author), van der Wielen, N. (author), van der Meulen, J. (author), Schuurman, T. (author), Witkamp, R.F. (author), Hendriks, H.F.J. (author), Koopmans, S.J. (author)The vagal nerve and gut hormones CCK and GLP-1 play important roles in the control of food intake. However, it is not clear to what extent CCK and GLP-1 increase satiation by stimulating receptors located on abdominal vagal nerve endings or via receptors located elsewhere. This study aimed to further explore the relative contribution of the...article 2015
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van Avesaat, M. (author), Troost, F.J. (author), Ripken, D. (author), Hendriks, H.F. (author), Aam, M. (author)BACKGROUND: Activation of the ileal brake, by infusing lipid directly into the distal part of the small intestine, alters gastrointestinal (GI) motility and inhibits food intake. The ileal brake effect on eating behavior of the other macronutrients is currently unknown. OBJECTIVE: The objective of this study was to investigate the effects of...article 2015
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Lukovac, S. (author), Roeselers, G. (author)When it comes to studying the effect of food bioactives on gut health, one of the essential steps that needs to be assessed is characterizing specific effects of the bioactives on the physical barrier of the lumen, the gastrointestinal tissue. In addition to studying the effects on transport function (e.g. by using Ussing chambers or cell...bookPart 2015
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Westerhout, J. (author), Wortelboer, H. (author), Verhoeckx, K. (author)The Ussing chamber system is named after the Danish zoologist Hans Ussing, who invented the device in the 1950s to measure the short-circuit current as an indicator of net ion transport taking place across frog skin (Ussing and Zerahn, Acta Physiol Scand 23:110-127, 1951). Ussing chambers are increasingly being used to measure ion transport in...bookPart 2015
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- Aguirre, M. (author), Venema, K. (author) article 2015
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Aguirre, M. (author), Venema, K. (author)Recently, a great deal of interest has been expressed regarding strategies to tackle worldwide obesity because of its accelerated wide spread accompanied with numerous negative effects on health and high costs. Obesity has been traditionally associated with an imbalance in energy consumed when compared to energy expenditure. However, growing...article 2015