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Cytokines and atherosclerosis: A comprehensive review of studies in mice

Kleemann, R. (author), Zadelaar, A.S.M. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)

In the past few years, inflammation has emerged as a major driving force of atherosclerotic lesion development. It is now well-established that from early lesion to vulnerable plaque formation, numerous cellular and molecular inflammatory components participate in the disease process. The most prominent cells that invade in evolving lesions are...

article 2008

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Atherosclerosis and liver inflammation induced by increased dietary cholesterol intake: A combined transcriptomics and metabolomics analysis

Kleemann, R. (author), Verschuren, L. (author), van Erk, M.J. (author), Nikolsky, Y. (author), Cnubben, N.H.P. (author), Verheij, E.R. (author), Smilde, A.K. (author), Hendriks, H.F.J. (author), Zadelaar, A.S.M. (author), Smith, G.J. (author), Kaznacheev, V. (author), Nikolskaya, T. (author), Melnikov, A. (author), Hurt-Camejo, E. (author), van der Greef, J. (author), van Ommen, B. (author), Kooistra, T. (author), TNO Kwaliteit van Leven (author)

Background: Increased dietary cholesterol intake is associated with atherosclerosis. Atherosclerosis development requires a lipid and an inflammatory component. It is unclear where and how the inflammatory component develops. To assess the role of the liver in the evolution of inflammation, we treated ApoE* 3Leiden mice with cholesterol-free ...

article 2007

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Interleukin 4 and prolonged hypoxia induce a higher gene expression of lysyl hydroxylase 2 and an altered cross-link pattern: Important pathogenetic steps in early and late stage of systemic scleroderma?

Brinckmann, J. (author), Kim, S. (author), Wu, J. (author), Reinhardt, D.P. (author), Batmunkh, C. (author), Metzen, E. (author), Notbohm, H. (author), Bank, R.A. (author), Krieg, T. (author), Hunzelmann, N. (author), TNO Kwaliteit van Leven (author)

The major pathological processes of systemic scleroderma (SSc) comprise inflammation and microvascular damage in the early or acute progressive stage as well as tissue fibrosis and hypoxia in the chronic end stage. Fibrosis seems to be a general phenomenon characterized by an increase of hydroxylysine aldehyde derived collagen cross-links which...

article 2005

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Endotoxin-induced liver damage in rats is minimized by β2- adrenoceptor stimulation

Izeboud, C.A. (author), Hoebe, K.H.N. (author), Grootendorst, A.F. (author), Nijmeijer, S.M. (author), van Miert, A.S. (author), Witkamp, R.F. (author), Rodenburg, R.J.T. (author), TNO Voeding (author)

Objective and Design: To investigate the effects of β2- adrenoceptor (β2-AR) stimulation on endotoxin-induced liver damage and systemic cytokine levels in rats. Subjects: Standard male Wistar rats. Treatment: A disease-model of lipolysaccharide (LPS)-induced acute systemic inflammation was used. The β2-selective AR agonist clenbuterol was...

article 2004

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Evidence for anti-inflammatory activity of statins and PPARα activators in human C-reactive protein transgenic mice in vivo and in cultured human hepatocytes in vitro

Kleemann, R. (author), Verschuren, L. (author), de Rooij, B.J. (author), Lindeman, J. (author), de Maat, M.M. (author), Szalai, A.J. (author), Princen, H.M.G. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)

Inflammatory processes, aside from cholesterol, play a central role in atherogenesis. Human C-reactive protein (huCRP) signals systemic inflammation and independently predicts future cardiovascular risk. Cholesterol-lowering statins reduce atherosclerosis and plasma huCRP levels. Evidence is sought for a direct anti-inflammatory statin effect in...

article 2004

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Fibrates down-regulate IL-1-stimulated C-reactive protein gene expression in hepatocytes by reducing nuclear p50-NFκB-C/EBP-β complex formation

Kleemann, R. (author), Gervois, P.P. (author), Verschuren, L. (author), Staels, B. (author), Princen, H.M.G. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)

C-reactive protein (CRP) is a major acute-phase protein in humans. Elevated plasma CRP levels are a risk factor for cardiovascular disease. CRP is predominantly expressed in hepatocytes and is induced by interleukin-1 (IL-1) and IL-6 under inflammatory situations, such as the acute phase. Fibrates are hypolipidemic drugs that act through the...

article 2003

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Expression of the IL-10 receptor on human monocytes is moderately suppressed by glucocorticoids

TNO Preventie en Gezondheid (author), Visser, J. (author), Nagelkerken, L. (author)

Chemicals/CAS: Dexamethasone, 50-02-2; Receptors, Interleukin; Receptors, Interleukin-10.

article 2002

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A role for NF-KB-dependent gene transactivation in sunburn

Abeyama, K. (author), Eng, W. (author), Jester, J.V. (author), Vink, A.A. (author), Edelbaum, D. (author), Cockerell, C.J. (author), Bergstresser, P.R. (author), Takashima, A. (author)

Exposure of skin to ultraviolet (UV) radiation is known to induce NF-κB activation, but the functional role for this pathway in UV-induced cutaneous inflammation remains uncertain. In this study, we examined whether experimentally induced sunburn reactions in mice could be prevented by blocking UV-induced, NF-κB-dependent gene transactivation...

article 2000

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On the role of c-Jun in the induction of PAI-1 gene expression by phorbol ester, serum, and IL-1α in HepG2 cells

Arts, J. (author), Grimbergen, J. (author), Toet, K. (author), Kooistra, T. (author), Gaubius instituut TNO (author)

We have characterized the regulation of plasminogen activator inhibitor- 1 (PAI-1) gene expression by phorbol 12-myristate 13-acetate (PMA), serum, and interleukin-1α (IL-1α) in the human hepatoma cell line HepG2. PMA, serum, and IL-1α induced a rapid and transient 28-fold (PMA), 9-fold (serum), and 23-fold (IL-1α) increase in PAI-1 mRNA,...

article 1999

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Superinduction of interleukin 8 mRNA in activated monocyte derived macrophages from rheumatoid arthritis patients

Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Rodenburg, R.J.T. (author), van den Hoogen, F.H.J. (author), Barrera, P. (author), van Venrooij, W.J. (author), van de Putte, L.B.A. (author)

article 1999

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Matrix degradation by chondrocytes cultured in alginate: IL-1β induces proteoglycan degradation and proMMP synthesis but does not result in collagen degradation

Beekman, B. (author), Verzijl, N. (author), de Roos, J.A.D.M. (author), TeKoppele, J.M. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)

Objective: To determine the role of interleukin-1β (IL-1β) in the degradation of proteoglycans and collagen by articular chondrocytes. Design: Chondrocytes were cultured in alginate beads for 2 weeks to produce extracellular matrix, followed by the addition of IL-1β for 1 or 2 days. Breakdown of extracellular matrix (with and without activation...

article 1998

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Expression of accessory molecules and cytokines in acute EAE in marmoset monkeys (Callithrix jacchus)

Laman, J.D. (author), van Meurs, M. (author), Schellekens, M.M. (author), de Boer, M. (author), Melchers, B. (author), Massacesi, L. (author), Lassmann, H. (author), Claassen, E. (author), 't Hart, B.A. (author), TNO Preventie en Gezondheid (author)

Accessory molecules and cytokines are involved in the immunopathogenesis of multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE) in rodent models, and are potential targets for immunotherapy. Evaluation of such experimental therapies requires appropriate animal models. Therefore, we analysed the expression of selected...

article 1998

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Effects of verocytotoxin-1 on nonadherent human monocytes: Binding characteristics, protein synthesis, and induction of cytokine release

van Setten, P.A. (author), Monnens, L.A.H. (author), Verstraten, R.G.G. (author), van den Heuvel, L.P.W.J. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)

The epidemic form of the hemolytic uremic syndrome (HUS) has been associated with a verocytotoxin producing Escherichia coli infection. Endothelial cell damage of glomeruli and arterioles of the kidney plays a central role in the pathogenesis of HUS. A number of observations in vivo and in vitro indicate that inflammatory mediators contribute to...

article 1996

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Tumor necrosis factor α induces endothelial galactosyl transferase activity and verocytotoxin receptors. Role of specific tumor necrosis factor receptors and protein kinase C

van de Kar, N.C.A.J. (author), Kooistra, T. (author), Vermeer, M. (author), Lesslauer, W. (author), Monnens, L.A.H. (author), van Hinsbergh, V.W.M. (author), TNO Preventie en Gezondheid (author)

Infections with verocytotoxin (VT) producing Escherichia coil have been strongly implicated in the epidemic form of hemolytic uremic syndrome (HUS). Endothelial damage plays a central role in the pathogenesis of HUS. In vitro studies have shown that VT can damage endothelial cells after interaction with its cellular receptor...

article 1995

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Regulation of plasminogen activation, matrix metalloproteinases and urokinase-type plasminogen activator-mediated extracellular matrix degradation in human osteosarcoma cell line MG63 by interleukin-1 alpha

de Bart, A.C.W. (author), Quax, P.H.A. (author), Löwik, C.W.G.M. (author), Verheijen, J.H. (author), TNO Preventie en Gezondheid (author)

Plasmin-mediated extracellular proteolysis has been implicated in the degradation of bone in normal and pathological conditions. Normal and malignant osteoblasts can produce both tissue-type plasminogen activator (t- PA) and urokinase-type plasminogen activator (u-PA). We have used the osteosarcoma cell line MG63 to address the question of...

article 1995

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Inhibiting interleukin-1 and tumor necrosis factor-α does not reduce induction of plasminogen activator inhibitor type-1 by endotoxin in rats in vivo

Emeis, J.E. (author), Hoekzema, R. (author), de Vos, A.F. (author), TNO Preventie en Gezondheid (author)

In experimental animals and humans, intravenous (IV) injection of endotoxin induces large increases in circulating plasminogen activator inhibitor type-1 (PAI-1), a major inhibitor of blood fibrinolysis. A similar increase is seen after the injection of interleukin-1 (IL-1) or of tumor necrosis factor-α (TNF-α), suggesting that these cytokines...

article 1995

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Epidermal cell proliferation and terminal differentiation in skin organ culture after topical exposure to sodium dodecyl sulphate

van de Sandt, J.J.M. (author), Bos, T.A. (author), Rutten, A.A.J.J.L. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)

Epidermal cell proliferation and differentiation were investigated in vitro after exposure to the anionic surfactant sodium dodecyl sulfate (SDS). Human skin organ cultures were exposed topically to various concentrations of SDS for 22 h, after which the irritant was removed. Cell proliferation was measured immunohistochemically by incorporation...

article 1995

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Role of opioid peptides in the regulation of cytokine production by murine CD4+ T cells

Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author), van den Bergh, P. (author), Dobber, R. (author), Ramlal, S. (author), Rozing, J. (author), Nagelkerken, L. (author)

The presence of the opioid peptides α- and β-endorphin (-End) but not methionine enkephalin (Met-enk) in in vitro cultures of purified CD4+ T cells, stimulated with concanavalin A in the presence of irradiated spleen cells, resulted in a threefold stimulation of IL-2, IL-4, and IFN-γ production. The stimulating effect was dependent on the...

article 1994

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Factors affecting the transduction of pluripotent hematopoietic stem cells: Long-term expression of a human adenosine deaminase gene in mice

Einerhand, M.P.W. (author), Bakx, T.A. (author), Kukler, A. (author), Valerio, D. (author), Radiobiologisch Instituut TNO (author)

An amphotropic retroviral vector, LgAL(ΔMo + PyF101) containing a human adenosine deaminase (ADA) cDNA was used to optimize procedures for the lasting genetic modification of the hematopoietic system of mice. The highest number of retrovirally infected cells in the hematopoietic tissues of long- term reconstituted mice was observed after...

article 1993

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Regulation of matrix metalloproteinase expression in human vein and microvascular endothelial cells: Effects of tumour necrosis factor α, interleukin 1 and phorbol ester

Gaubius Instituut TNO (author), Hanemaaijer, R. (author), Koolwijk, P. (author), Le Clercq, L. (author), de Vree, W.J.A. (author), van Hinsbergh, V.W.M. (author)

Matrix metalloproteinases (MMPs) play a role in tissue remodelling and angiogenesis. We have investigated the expression and regulation of MMP-1 (interstitial collagenase), MMP-2 (gelatinase A), MMP-3 (stromelysin 1), MMP-7 (matrilysin), MMP-9 (gelatinase B) and their inhibitors TIMP-1 and TIMP-2 in human umbilical vein, femoral vein and...

article 1993