Suitability of PER.C6 cells to generate epidemic and pandemic influenza vaccine strains by reverse genetics

Reverse genetics, the generation of influenza viruses from cDNA, presents a rapid method for creating vaccine strains. The technique necessitates the use of cultured cells. Due to technical and regulatory requirements, the choice of cell lines for production of human influenza vaccines is limited. PER.C6® cells, among the most extensively...

Lactobacilli as live vaccine delivery vectors: Progress and prospects

Evidence is accumulating that lactobacilli influence the immune response in a strain-dependent manner. This immunomodulatory capacity is important for the development of the immune response, and also identifies Lactobacillus as a potent oral vaccine carrier. Most of our current knowledge of the use of lactobacilli for vaccination purposes has...

Correlation between the antibody response to influenza vaccine and helper T cell subsets in healthy ageing

To investigate the effects of the altered composition of the helper T cell compartment in ageing on the humoral response to influenza vaccine, we investigated correlations between helper T cell subsets and anti-influenza antibody responses in 23 JUNIEUR healthy young and 41 SENIEUR healthy elderly subjects. Naive helper T cell numbers (CD4...

The in vivo effects of neutralizing antibodies against IFN-γ, IL-4, or IL-10 on the humoral immune response in young and aged mice

In the present study we investigated whether age-related changes in the composition and functional properties of murine CD4+ T cells are reflected in vivo by a changed humoral response to influenza vaccine in aged mice. After the primary immunization, the titers of influenza-specific IgM, IgG1, IgG2a, and IgG2b, but not of IgG3 and IgE, were...

Verhoging van de vaccinatiegraad tegen influenza bij risicopatiënten door gerichte uitnodiging in de eerste lijn [Increase of the influenza vaccination coverage of at-risk patients by personal invitation in primary health care]

Objective. To increase the coverage of influenza vaccination by a programmatic approach in a large population, aimed at the general practitioners (GPs). Design. Intervention study (prospective). Setting. The region of central Brabant (Tilburg and some surrounding communities), the Netherlands. Method. The intervention was carried out in a...

Clonal antibody dominance after influenza vaccination in IgA nephropathy patients and controls

Chemicals/CAS: hemagglutinin, 37333-12-3; sialidase, 9001-67-6; Antibodies, Monoclonal; Antibodies, Viral; Hemagglutinins, Viral; Immunoglobulin A; Immunoglobulin G; Influenza Vaccine; Neuraminidase, EC 3.2.1.18