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Polak, R.L. (author), Molenaar, P.C. (author), van Gelder, M. (author), Medisch Biologisch Laboratorium RVO-TNO (author)The uptake of [14C]choline was studied in cortical slices from rat brain after their incubation in a Krebs-Henseleit medium containing either 4.7 mM-KCl (low K), 25 mM-KCl (high K) or 25 mM-KCl without calcium (Ca free, high K). With 0.84 μM-[14C]choline in the medium the uptake per gram of tissue was 0.62 nmol after incubation in low K medium,...article 1977
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- Lohman, P.H.M. (author), Reynolds, R. (author), Paterson, M.C. (author), Medisch Biologisch Laboratorium TNO (author) article 1977
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de Mik, G. (author), de Groot, I. (author), Gerbrandy, J.L.F. (author), Medisch Biologisch Laboratorium TNO Chemisch Laboratorium TNO (author)The effects of ozone and ozonized olefins on aerosol survival of bacteriophage phiX174 were studied. The ozone concentrations used were between 0 and 110 parts/109, giving decay rates up to 0.03 min-1. The olefins used were trans 2 butene and cyclohexene in concentrations of 500 parts/109 and 2.4 parts/106, respectively. Olefins alone have no...article 1977
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Kluft, C. (author), Jenks, R.L. (author), Astrup, T. (author), Gaubius instituut TNO (author)The authors studied the production of fibrinolytic activity in Hageman trait plasma, and conclude that the development of activator activity in euglobulin fractions is delayed, particularly in the Hageman trait plasma, by the presence of C1 inactivator, an inhibitor whose effect can be abolished by the synthetic compound flufenamic acid. Using...article 1977
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Gaubius instituut TNO (author), van Gent, C.M. (author), van der Voort, H.A. (author), de Bruyn, A.M. (author), Klein, F. (author)Two enzymatic methods for the determination of cholesterol in serum have been modified for automatic use. These methods utilize the colour reactions according to Kageyama or Trinder to measure the hydrogen peroxide resulting from the enzymatic reaction. The procedures have been compared with the classical methods according to Abell and to Huang....article 1977
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Veldhuisen, G. (author), Bacchetti, S. (author), van der Valk, M.J. (author), Medisch Biologisch Laboratorium TNO (author)Chemicals/CAS: DNA, Viralarticle 1976
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Davidse, L.C. (author), Werkgroep voor Inwendige Therapie van Plantenziekten TNO (author)Methyl benzimidazol 2 yl carbamate was metabolized by Aspergillus nidulans mycelium to two metabolites, one of which was identified as methyl 5 hydroxybenzimidazol 2 yl carbamate. This compound was further converted to a second metabolite which was not identified. Conversion rate was highest when the culture medium was depleted of nutrients....article 1976
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Lubbe, F.H. (author), Rossi, G. (author), Zaalberg, O.B. (author), Medisch Biologisch Laboratorium TNO (author)A method is described for isolation of antibody forming cells with the help of cluster formation. This was achieved by incubating spleen cells from immunized mice with sheep erythrocytes at 37°C. During incubation rabbit anti mouse immunoglobulin serum was present in order to facilitate the formation of clusters. Thereafter the clusters were...article 1976
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Vos, O. (author), Budke, L. (author), Grant, G.A. (author), Medisch Biologisch Laboratorium TNO (author)In tissue culture, protection against X irradiation by a number of cysteamine derivatives was studied and the results were compared with data obtained in mice. Compounds with a covered SH group, like WR 638, cysteamine phosphate, WR 2721 and AE AE 48527, showed practically no protection when dissolved in tissue culture medium, but developed a...article 1976
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Pouwels, P.H. (author), Pannekoek, H. (author), Medisch Biologisch Laboratorium TNO Chemisch Laboratorium TNO (author)Chemicals/CAS: tryptophan, 6912-86-3, 73-22-3; RNA, Bacterial; RNA, Viral; Tryptophan, 73-22-3; Tryptophan-tRNA Ligase, EC 6.1.1.2article 1976
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- Mattern, I.E. (author), Medisch Biologisch Laboratorium RVO-TNO (author) article 1976
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Nickolson, V.J. (author), Wolthuis, O.L. (author), Medisch Biologisch Laboratorium TNO (author)The effects of Piracetam, Naftidrofuryl and methamphetamine on several parameters of cerebral energy metabolism have been studied. At variance with some reports in the literature neither Piracetam nor Naftidrofuryl affected the cerebral contents of adenine nucleotides and, accordingly, both substances were without effect on the adenylate energy...article 1976
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Nickolson, V.J. (author), Wolthuis, O.L. (author), Medisch Biologisch Laboratorium TNO (author)The effect of Piracetam on rat cerebral protein metabolism in vivo and in vitro was studied. It was found that the drug stimulates the uptake of labelled leucine by cerebral cortex slices, has no effect on the incorporation of leucine into cerebral protein, neither in slices nor in vivo, but inhibits the breakdown of newly formed protein in...article 1976
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Vos, O. (author), Grant, G.A. (author), Budke, L. (author), Medisch Biologisch Laboratorium RVO-TNO (author)The effect of diamide on the radiosensitivity of T cells was investigated under oxic and anoxic conditions. The compound was found to sensitize the cells under both conditions. Under oxic conditions, exposure for 10 min before and during irradiation to 0.1, 0.5 and 1.0 mM diamide produced dose modifying factors of 0.81, 0.60 and 0.55,...article 1976
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van Hemmen, J.J. (author), Meuling, W.J.A. (author), Bleichrodt, J.F. (author), Medisch Biologisch Laboratorium TNO (author)The radiosensitization by oxygen of biologically active bacteriophage DNA in bacterial extracts was studied. The oxygen effect in such a system appeared not to be due or only to a minor extent to the presence of endogenous sulphydryl compounds. The components in a cell extract which enable oxygen and other sensitizers to sensitize DNA could not...article 1974
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Bleichrodt, J.F. (author), Verheij, W.S.D. (author), Medisch Biologisch Laboratorium TNO (author)Amber mutants of bacteriophage can be reverted by ionizing radiation to pseudo wild type particles, i.e. particles able to propagate in a suppressorless host. Since the DNA of phi X 174 is single stranded mutation cannot be due to errors in repair of radiation damage by the excision repair or by the recombination repair system of the host cell,...article 1973
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Molenaar, P.C. (author), Polak, R.L. (author), Nickolson, V.J. (author), Medisch Biologisch Laboratorium TNO (author)Slices from rat brain cortex were incubated for either 5 or 60 min in a medium containing [3H]choline and 4.7 or 25 mM KCl. Bioassayable ACh and labelled ACh were determined in the incubation medium, in the total tissue homogenate and in subcellular fractions. Raising the KCl concentration from 4.7 to 25 mM stimulated the release and synthesis...article 1973
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Medisch Biologisch Labortorium TNO (author), Kleijer, W.J. (author), Lohman, P.H.M. (author), Mulder, M.P. (author), Bootsma, D. (author)Repair replication and rejoining of single-strand breaks after X-irradiation in human-skin fibroblasts from normal donors and several patients with xeroderma pigmentosum have been compared. The xeroderma strains showed different levels of repair replication following UV exposure. Repair replication and rejoining of breaks, which are considered...article 1970
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Medisch Biologisch Labortorium TNO (author), Galavazi, G. (author), Bootsma, D. (author)Some aspects of the population dynamics before, during and after the double thymidine treatment synchronization procedure were analysed. The mitotic index in the synchronized populations rose from 0.0 per cent at 1 hr after the end of the second treatment to 20.8 per cent at 9.7 hr, dropped to 0.1 per cent at about 22 hr and increased again to a...article 1966
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Medisch Biologisch Labortorium TNO (author), Galavazi, G. (author), Schenk, H. (author), Bootsma, D. (author)An optimal procedure to synchronize the life cycles of the individuals in a population of human cells, cultured in vitro, by repeated temporary inhibition of the DNA synthesis was elaborated. Cultures in the logarithmic growth phase were treated three times with 7.5 mM thymidine dissolved in medium. Each treatment was terminated after 24 hr by...article 1966