a2-Antiplasmin Enschede: Dysfunctional alpha 2-antiplasmin molecule associated with an autosomal recessive hemorrhagic disorder

??2-Antiplasmin (??2-AP) is a major fibrinolysis inhibitor, whose complete, congenital absence has been found to be associated with a distinct hemorrhagic diathesis. We studied a 15-yr-old male with a hemorrhagic diathesis after trauma from early childhood on. This bleeding tendency was associated with a minimal ??2-AP level recorded...

Demonstration of two forms of A2-antiplasmin in plasma by modified crossed immunoelectrophoresis

A procedure to demonstrate two forms of ??2-antiplasmin in plasma, a plasminogen-binding and a non-plasminogen-binding one, is described. The procedure involves crossed immunoelectrophoresis using antiserum against ??2-antiplasmin, with, in the first dimension electrophoresis, lys-plasminogen added to the plasma and the agarose gel. A discrete...