Searched for: subject:"Immunochemistry"
(1 - 10 of 10)
document
Apostolovic, D. (author), Luykx, D. (author), Warmenhoven, H. (author), Verbart, D. (author), Stanic-Vucinic, D. (author), de Jong, G.A.H. (author), Velickovic, T.C. (author), Koppelman, S.J. (author)
Conglutins, the major peanut allergens, Ara h 2 and Ara h 6, are highly structured proteins stabilized by multiple disulfide bridges and are stable towards heat-denaturation and digestion. We sought a way to reduce their potent allergenicity in view of the development of immunotherapy for peanut allergy. Isoforms of conglutin were purified,...
article 2013
document
Snopov, S.A. (author), de Gruijl, F.R. (author), Roza, L. (author), van der Leun, J.C. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
Studies of UV-induced skin cancers show that malignisation of skin cells, as well as alterations in anti-tumor immune control, are triggered by UV-induced lesions in cellular DNA. Such lesions can probably appear in the human mononuclear leukocytes (lymphocytes) during exposure of skin to sunlight. With the aim of studying the processing of UV...
article 2004
document
van der Gaag, B. (author), Spath, S. (author), Dietrich, H. (author), Stigter, E. (author), Boonzaaijer, G. (author), van Osenbruggen, T. (author), Koopal, K. (author)
An immunochemical biosensor assay for the detection of multiple mycotoxins in a sample is described.The inhibition assay is designed to measure four different mycotoxins in a single measurement, following extraction, sample clean-up and incubation with an appropriate cocktail of anti-mycotoxin antibodies.The different mycotoxins could be...
article 2003
document
van der Schans, G.P. (author), Noort, D. (author), Mars-Groenendijk, R.H. (author), Fidder, A. (author), Chau, L.F. (author), de Jong, L.P.A. (author), Benschop, H.P. (author), Prins Maurits Laboratorium TNO (author)
As part of a program to develop methods for diagnosis of exposure to chemical warfare agents, we developed immunochemical methods for detection of adducts of sulfur mustard to keratin in human skin. Three partial sequences of keratins containing glutamine or asparagine adducted with a 2-hydroxyethylthioethyl group at the ω-amide function were...
article 2002
document
Noort, D. (author), Benschop, H.P. (author), Black, R.M. (author), Prins Maurits Laboratorium TNO (author)
In this report an overview of the methods currently available for detection of exposure to a number of chemical warfare agents (CWA), i.e., sulfur mustard, lewisite and nerve agents, is presented. Such methods can be applied for various purposes, e.g., diagnosis and dosimetry of exposure of casualties, confirmation of nonexposure, verification...
article 2002
document
van der Schans, G.P. (author), Timmerman, A.J. (author), Bruijnzeel, P.L.B. (author)
An immunochemical assay has been adapted to detect DNA damage in whole blood at biologically relevant doses of ionizing radiation. Upon alkaline treatment of whole blood, both strand breaks and base damage (which is converted into strand breaks by the addition of damage-specific enzymes) are detected by using antibodies that specifically bind to...
article 2002
document
Koppelman, S. (author), Knulst, A.C. (author), Koers, W.J. (author), Penninks, A.H. (author), Peppelman, H. (author), Vlooswijk, R. (author), Pigmans, I. (author), van Duijn, G. (author), Hessing, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Hazelnuts are widely used in the food industry owing to their nutritive value and taste. The amount of hazelnut present in a recipe is usually considered as a mark of quality. On the other hand, contamination of foods that normally do not contain hazelnuts is a threat for patients with a hazelnut allergy. For this reason, the availability of a...
article 1999
document
Morse, D.C. (author), Plug, A. (author), Wesseling, W. (author), van den Berg, K.J. (author), Brouwer, A. (author), TNO Voeding (author)
Pregnant Wistar WU rats were exposed to 0, 5, and 25 mg of the commercial polychlorinated biphenyl (PCB) mixture Aroclor 1254 per kilogram of body weight on Days 10 to 16 of gestation. Pregnant rats were sacrificed on Gestation Day 20 to observe effects on fetal body and brain weights. Male and female offspring were sacrificed on Postnatal Days...
article 1996
document
van Loon, A.A.W.M. (author), Timmerman, A.J. (author), van der Schans, G.P. (author), Lohman, P.H.M. (author), Baan, R.A. (author), Medisch Biologisch Laboratorium TNO (author)
Exposure of cells to ionizing radiation gives rise to DNA damage, comprising strand breaks and base modifications. All these lesions may contribute to cell death, mutagenesis and/or carcinogenesis, but their relative contributions are likely to be different. It is important, therefore, to study the various damages with respect to their abundance...
article 1992
document
Kluft, C. (author), Jie, A.F.H. (author), Sprengers, E.D. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)
Inhibition of tissue-type plasminogen activator (t-PA) by pooled plasma could be ascribed for only 60% to the endothelial cell type PA inhibitor. The residual inhibition is ascribed to a so-far undescribed plasma component present at 0.2 nmol/l. This component shows reversible binding to t-PA with an apparent K(i) of 10 pmol/l (does not hinder t...
article 1985
Searched for: subject:"Immunochemistry"
(1 - 10 of 10)