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The Core Human Microbiome: Does It Exist and How Can We Find It? A Critical Review of the Concept

Selberherr, E. (author), Grau, J.H. (author), Meixner, M. (author), Liere, K. (author), Ercolini, D. (author), de Filippo, C. (author), Caderni, G. (author), Brigidi, P. (author), Turroni, S. (author)

The core microbiome, which refers to a set of consistent microbial features across populations, is of major interest in microbiome research and has been addressed by numerous studies. Understanding the core microbiome can help identify elements that lead to dysbiosis, and lead to treatments for microbiome-related health states. However, defining...

article 2022

document

The Core Human Microbiome: Does It Exist and How Can We Find It? A Critical Review of the Concept

Sharon, I. (author), Quijada, N.M. (author), Pasolli, E. (author), Fabbrini, M. (author), Vitali, F. (author), Agamennone, V. (author), Dötsch, A. (author), Selberherr, E. (author), Grau, J.H. (author), Meixner, M. (author), Liere, K. (author), Ercolini, D. (author), de Filippo, C. (author), Caderni, G. (author), Brigidi, P. (author), Turroni, S. (author)

The core microbiome, which refers to a set of consistent microbial features across populations, is of major interest in microbiome research and has been addressed by numerous studies. Understanding the core microbiome can help identify elements that lead to dysbiosis, and lead to treatments for microbiome-related health states. However, defining...

article 2022

document

Could 3D food printing help to improve the food supply chain resilience against disruptions such as caused by pandemic crises?

Derossi, A. (author), Bhandari, B. (author), van Bommel, K. (author), Noort, M. (author), Severini, C. (author)

The ongoing COVID-19 pandemic is having a tremendous effect on the current food system. The situation urges us to face many issues never experienced before, aimed at mitigating a multitude of sanitary and social risks. The current pandemic has affected the food system in many ways: significant changes in dietary habits and in the health status...

article 2021

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Nanomaterials and the serosal immune system in the thoracic and peritoneal cavities

Kuper, C.F. (author), Pieters, R.H.H. (author), van Bilsen, J.H.M. (author)

The thoracic and peritoneal cavities are lined by serous membranes and are home of the serosal immune system. This immune system fuses innate and adaptive immunity, to maintain local homeostasis and repair local tissue damage, and to cooperate closely with the mucosal immune system. Innate lymphoid cells (ILCs) are found abundantly in the...

article 2021

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Seeking Windows of Opportunity to Shape Lifelong Immune Health A Network-Based Strategy to Predict and Prioritize Markers of Early Life Immune Modulation

van Bilsen, J.H.M. (author), Dulos, R. (author), van Stee, M.F. (author), Meima, M.Y. (author), Rouhani Rankouhi, T. (author), Neergaard Jacobsen, L. (author), Staudt Kvistgaard, A. (author), Garthoff, J.A. (author), Knippels, L.M.J. (author), Knipping, K. (author), Houben, G.F. (author), Verschuren, L. (author), Meijerink, M. (author), Krishnan, S. (author)

A healthy immune status is strongly conditioned during early life stages. Insights into the molecular drivers of early life immune development and function are prerequisite to identify strategies to enhance immune health. Even though several starting points for targeted immune modulation have been identified and are being developed into...

article 2020

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Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies

Lozano-Ojalvo, D. (author), Benede, S. (author), Antunes, C.M. (author), Bavaro, S.L. (author), Bouchaud, G. (author), Costa, A. (author), Denery-Papini, S. (author), Diaz-Perales, A. (author), Garrido-Arandia, M. (author), Gavrovic-Jankulovic, M. (author), Hayen, S. (author), Martinez-Blanco, M. (author), Molina, E. (author), Monaci, L. (author), Pieters, R.H.H. (author), Villemin, C. (author), Wichers, H.J. (author), Wroblewska, B. (author), Willemsen, L.E.M. (author), Roggen, E.L. (author), van Bilsen, J.H.M. (author)

Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the...

article 2019

document

The serosal immune system of the thorax in toxicology

Kuper, C.F. (author), van Bilsen, J. (author), Wijnands, M.V.W. (author)

The thoracic cavities receive increasing attention in toxicology, because inhaled fibers and (nano)particles can reach these cavities and challenge the local lymphoid tissues. The thoracic and abdominopelvic cavities are controlled by the serosal immune system with its special, loosely organized lymphoid clusters, namely the fat-associated...

article 2018

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Moderate alcohol consumption after a mental stressor attenuates the endocrine stress response

Schrieks, I.C. (author), Joosten, M.M. (author), Klöpping-Ketelaars, W.A.A. (author), Witkamp, R.F. (author), Hendriks, H.F.J. (author)

Alcohol is often consumed to reduce tension and improve mood when exposed to stressful situations. Previous studies showed that moderate alcohol consumption may reduce stress when alcohol is consumed prior to a stressor, but data on the effect of alcohol consumption after a mental stressor is limited. Therefore, our objective was to study...

article 2016

document

Inflammatory Cells in Patients with Endstage Knee Osteoarthritis: A Comparison between the Synovium and the Infrapatellar Fat Pad

Klein-Wieringa, I.R. (author), de Lange-Brokaar, B.J. (author), Yusuf, E. (author), Andersen, S.N. (author), Kwekkeboom, J.C. (author), Kroon, H.M. (author), van Osch, G.J. (author), Zuurmond, A.M. (author), Stojanovic-Susulic, V. (author), Nelissen, R.G. (author), Toes, R.E. (author), Kloppenburg, M. (author), Ioan-Facsinay, A. (author)

Objective: To get a better understanding of inflammatory pathways active in the osteoarthritic (OA) joint, we characterized and compared inflammatory cells in the synovium and the infrapatellar fat pad (IFP) of patients with knee OA. Methods: Infiltrating immune cells were characterized by flow cytometry in 76 patients with knee OA (mean age 63...

article 2016

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The advantage of laser-capture microdissection over whole tissue analysis in proteomic profiling studies

de Marchi, T. (author), Braakman, R.B.H. (author), Stingl, C. (author), van Duijn, M.M. (author), Smid, M. (author), Foekens, J.A. (author), Luider, T.M. (author), Martens, J.W.M. (author), Umar, A. (author)

article 2016

document

Inflammation and nutritional science for programs/policies and interpretation of research evidence (INSPIRE)

Raiten, D.J. (author), Sakr Ashour, F.A. (author), Ross, A.C. (author), Meydani, S.N. (author), Dawson, H.D. (author), Stephensen, C.B. (author), Brabin, B.J. (author), Suchdev, P.S. (author), van Ommen, B. (author), Ahluwalia, N. (author), Ahmed, T. (author), Allen, L. (author), Beck, M. (author), Blanchard, H. (author), Brindle, E. (author), Brown, K. (author), Calder, P. (author), Checkley, W. (author), Claycombe, K. (author), Coles, C. (author), Esamai, F. (author), Friis, H. (author), Gardner, E. (author), Grinspoon, S. (author), Guerrant, R. (author), Hellerstein, M. (author), Hunt, J. (author), King, J. (author), Klasing, K. (author), Kramer, B. (author), Koski, K. (author), Northrop-Clewes, C. (author), Pfeiffer, C. (author), Prentice, A. (author), Ramirez-Zea, M. (author), van Rheenan, P. (author), Rink, L. (author), Srinivasa, S. (author), Benn, C.S. (author), Stephensen, C. (author), Suchdev, P. (author), Sullivan, K. (author), Thurnham, D. (author), Varadhan, R. (author), Van Der Ven, A. (author), Walston, J. (author), Xia, J. (author)

An increasing recognition has emerged of the complexities of the global health agenda-specifically, the collision of infections and noncommunicable diseases and the dual burden of over- and undernutrition. Of particular practical concern are both 1) the need for a better understanding of the bidirectional relations between nutritional status and...

article 2015

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The minipig as an alternative non-rodent model for immunogenicity testing using the TNFα blockers adalimumab and infliximab

van Mierlo, G.J.D. (author), Cnubben, N.H.P. (author), Wouters, D. (author), Wolbink, G.J. (author), Hart, M.H.L. (author), Rispens, T. (author), Ganderup, N.C. (author), Kuper, C.F. (author), Aarden, L. (author), Penninks, A.H. (author)

Immunogenicity is a major issue of concern for monoclonal antibodies used in human diseases and is by default mainly determined in non-human primates (NHP), as target molecules are considered most similar in NHP compared to human. In this manuscript the predictive value of immunogenicity testing in minipigs for human safety is evaluated, as the...

article 2014

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Immunologic activity of schistosomal and bacterial TLR2 ligands in Gabonese children

Retra, K. (author), van Riet, E. (author), Adegnika, A.A. (author), Everts, B. (author), van Geest, S. (author), Kremsner, P.G. (author), van Hellemond, J.J. (author), van der Kleij, D. (author), Tielens, A.G.M. (author), Yazdanbakhsh, M. (author), TNO Defensie en Veiligheid (author)

Schistosomes carry lipid moieties that interact with the immune system. To understand the consequence of interactions in terms of polarizing the cytokine profiles, the effect of two Toll-like receptor-2 (TLR2) activating schistosomal lipid fractions was studied on whole blood from Gabonese children living in a schistosomiasis endemic area. One...

article 2008

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Gaining Insight into Microbial Physiology in the Large Intestine: A Special Role for Stable Isotopes

de Graaf, A.A. (author), Venema, K. (author), TNO Kwaliteit van Leven (author)

The importance of the human large intestine for nutrition, health, and disease, is becoming increasingly realized. There are numerous indications of a distinct role for the gut in such important issues as immune disorders and obesity-linked diseases. Research on this long-neglected organ, which is colonized by a myriad of bacteria, is a rapidly...

conference paper 2008

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Vascular Growth in Ischemic Limbs: A Review of Mechanisms and Possible Therapeutic Stimulation

van Weel, V. (author), van Tongeren, R.B. (author), van Hinsbergh, V.W.M. (author), van Bockel, J.H. (author), Quax, P.H.A. (author), TNO Kwaliteit van Leven (author)

Stimulation of vascular growth to treat limb ischemia is promising, and early results obtained from uncontrolled clinical trials using angiogenic agents, e.g., vascular endothelial growth factor, led to high expectations. However, negative results from recent placebo-controlled trials warrant further research. Here, current insights into...

article 2008

document

Differentiation of primary adult microglia alters their response to TLR8-mediated activation but not their capacity as APC

Zuiderwijk-Sick, E.A. (author), van der Putten, C. (author), Bsibsi, M. (author), Deuzing, I.P. (author), de Boer, W. (author), Persoon-Deen, C. (author), Kondova, I. (author), Boven, L.A. (author), van Noort, J.M. (author), 't Hart, B.A. (author), Amor, S. (author), Bajramovic, J.J. (author), TNO Defensie en Veiligheid TNO Kwaliteit van Leven (author)

Activated microglia are found in a variety of neuroinflammatory disorders where they have attributed roles as effector as well as antigen-presenting cells (APC). Critical determinants for the multifaceted role of microglia are the differentiation potential of microglia and their mode of activation. In this study, we have investigated the effects...

article 2007

document

Initiating mechanisms of food allergy: Oral tolerance versus allergic sensitization

van Wijk, F. (author), Knippels, L. (author), TNO Kwaliteit van Leven (author)

Immediately after birth the mucosa of the gastrointestinal tract, which represents the greatest body surface area exposed to the outside environment, is confronted with a large variety of foreign antigens. The immune system of the intestine now has to meet the task of discriminating between pathogens and harmless antigens, such as food proteins...

article 2007

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Protective and therapeutic role for αB-crystallin in autoimmune demyelination

Ousman, S.S. (author), Tomooka, B.H. (author), van Noort, J.M. (author), Wawrousek, E.F. (author), O'Conner, K. (author), Hafler, D.A. (author), Sobel, R.A. (author), Robinson, W.H. (author), Steinman, L. (author), TNO Kwaliteit van Leven (author)

αB-crystallin (CRYAB) is the most abundant gene transcript present in early active multiple sclerosis lesions, whereas such transcripts are absent in normal brain tissue. This crystallin has anti-apoptotic and neuroprotective functions. CRYAB is the major target of CD4+ T-cell immunity to the myelin sheath from multiple sclerosis brain. The...

article 2007

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Autoantibodies against alpha B-crystallin, a candidate autoantigen in multiple sclerosis, are part of a normal human immune repertoire

van Noort, J.M. (author), Verbeek, R. (author), Meilof, J.F. (author), Polman, C.H. (author), Amor, S. (author), TNO Kwaliteit van Leven (author)

Human T-cell responses to the stress protein alpha B-crystallin in multiple sclerosis (MS)-affected brain samples are dominant when compared to other myelin antigens. The establishment of the apparent autoimmune repertoire against this antigen has been suggested to involve cross-priming during viral infection. Yet, another possibility would be...

article 2006

document

General aspects of immunotoxicology including validation issues

Frieke Kuper, C. (author), TNO Kwaliteit van Leven (author)

Histologic examination of lymphoid organs has revealed immunotoxic effects of a broad range of substances. The thymus has proven especially sensitive. The relative lack of sensitivity of mucosa-associated lymphoid cells and tissues may be due to shortcomings in the way they are examined. Validation of ways to examine mucosal lymphoid tissues and...

article 2006