Searched for: subject:"Hydrolysis"
(1 - 20 of 77)

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Segers, M. (author), Sliepen, M. (author), Kraft, D.J. (author), Möller, M. (author), Buskens, P. (author)
Recently, our group reported synthesis routes for sub-micron sized hollow, and nanoporous phenylsiloxane spheres, in which phenyltrimethoxysilane is applied as surfmer. Here, we present insights into the surfactant formed through hydrolytic conversion of phenyltrimethoxysilane, and factors influencing the particle architecture. cop. 2016...
article 2016
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Reinen, J. (author), Nematollahi, L. (author), Fidder, A. (author), Vermeulen, N.P.E. (author), Noort, D. (author), Commandeur, J.N.M. (author)
Tri-ortho-cresyl phosphate (ToCP) is a multipurpose organophosphorus compound that is neurotoxic and suspected to be involved in aerotoxic syndrome in humans. It has been reported that not ToCP itself but a metabolite of ToCP, namely, 2-(ortho-cresyl)-4H-1,2,3-benzodioxaphosphoran-2-one (CBDP), may be responsible for this effect as it can...
article 2015
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Verhoeckx, K.C.M. (author), Vissers, Y.M. (author), Baumert, J.L. (author), Faludi, R. (author), Feys, M. (author), Flanagan, S. (author), Herouet-Guicheney, C. (author), Holzhauser, T. (author), Shimojo, R. (author), van der Bolt, N. (author), Wichers, H. (author), Kimber, I. (author)
Food processing can have many beneficial effects. However, processing may also alter the allergenic properties of food proteins. A wide variety of processing methods is available and their use depends largely on the food to be processed. In this review the impact of processing (heat and non-heat treatment) on the allergenic potential of proteins...
article 2015
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Zune, Q. (author), Delepierre, A. (author), Gofflot, S. (author), Bauwens, J. (author), Twizere, J.C. (author), Punt, P.J. (author), Francis, F. (author), Toye, D. (author), Bawin, T. (author), Delvigne, F. (author)
Fungal biofilm is known to promote the excretion of secondary metabolites in accordance with solid-staterelated physiological mechanisms. This work is based on the comparative analysis of classical submerged fermentation with a fungal biofilmreactor for the production of a Gla::green fluorescent protein (GFP) fusion protein by Aspergillus oryzae...
article 2015
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Ahmadi-Abhari, S. (author), Woortman, A.J.J. (author), Oudhuis, A.A.C.M. (author), Hamer, R.J. (author), Loos, K. (author)
This study was aimed to assess the role of lysophosphatidylcholine (LPC) in the development of slowly digestible starch (SDS). The influence of LPC, on the enzymatic degradation of diluted 9% wheat starch suspensions (w/w) was investigated, using an in vitro digestion method. Wheat starch suspensions containing 0.5-5% LPC (based on starch) were...
article 2013
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ter Haar, R. (author), Timmermans, J.W. (author), Slaghek, T.M. (author), van Dongen, F.E.M. (author), Schols, H.A. (author), Gruppen, H. (author), TNO Kwaliteit van Leven (author)
Chemical derivatization is often applied to improve polysaccharide functionality. Primary hydroxyl groups in starch can, for example, be oxidized to aldehydes by using a 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-mediated reaction. The major part of the aldehydo groups is subsequently converted to carboxyl groups by NaOCl. The exact structure...
article 2010
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Adav, S.S. (author), Li, A.A. (author), Manavalan, A. (author), Punt, P. (author), Sze, S.K. (author), TNO Kwaliteit van Leven (author)
The natural lifestyle of Aspergillus niger made them more effective secretors of hydrolytic proteins and becomes critical when this species were exploited as hosts for the commercial secretion of heterologous proteins. The protein secretion profile of A. niger and its mutant at different pH was explored using iTRAQ-based quantitative proteomics...
article 2010
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Kong, H. (author), Wang, M. (author), Venema, K. (author), Maathuis, A. (author), van der Heijden, R. (author), van der Greef, J. (author), Xu, G. (author), Hankemeier, T. (author), TNO Kwaliteit van Leven (author)
A high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry (HPLC-FTICR-MS) method was developed to investigate the metabolism of ginsenosides in in vitro models of the gastro-intestinal tract. The metabolites were identified by high-resolution tandem mass spectrometry. Degradation and...
article 2009
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Adams, T.B. (author), Gavin, C.L. (author), Taylor, S.V. (author), Waddell, W.J. (author), Cohen, S.M. (author), Feron, V.J. (author), Goodman, J. (author), Rietjens, I.M.C.M. (author), Marnett, L.J. (author), Portoghese, P.S. (author), Smith, R.L. (author), TNO Kwaliteit van Leven (author), KvL (author)
This publication is the 12th in a series of safety evaluations performed by the Expert Panel of the Flavor and Extract Manufacturers Association (FEMA). In 1993, the Panel initiated a comprehensive program to re-evaluate the safety of more than 1700 GRAS flavoring substances under conditions of intended use. Since then, the number of flavoring...
article 2008
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Goosen, C. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)
The purified exo-inulinase enzyme of Aspergillus niger N402 (AngInuE; heterologously expressed in Escherichia coli) displayed a sucrose:inulin (S/I) hydrolysis ratio of 2.3, characteristic for a typical exo-inulinase. The enzyme also had significant transfructosylating activity with increasing sucrose concentrations, producing various...
article 2008
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van Boxtel, E.L. (author), Koppelman, S.J. (author), van den Broek, L.A.M. (author), Gruppen, H. (author), TNO Kwaliteit van Leven (author), KvL (author)
This study was aimed at the determination of the pepsin-susceptible and pepsin-resistant epitopes in native and heat-treated Ara h 1, a major allergen from peanuts. Both the oligomeric structure and the trimeric structure of the allergen were investigated. Under the in vitro conditions applied, oligomeric Ara h 1, either unheated or preheated,...
article 2008
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Stern, R. (author), Builtjes, P. (author), Schaap, M. (author), Timmermans, R. (author), Vautard, R. (author), Hodzic, A. (author), Memmesheimer, M. (author), Feldmann, H. (author), Renner, E. (author), Wolke, R. (author), Kerschbaumer, A. (author), TNO Bouw en Ondergrond (author)
Five three-dimensional chemical transport models of different complexity were applied to Central Europe to assess the ability of models to reproduce PM10 concentrations under highly polluted conditions. The participating models were the French CHIMERE model, the Dutch LOTOS-EUROS model, as well as the REM-CALGRID, the EURAD and the LM-MUSCAT...
article 2008
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TNO Kwaliteit van Leven (author), Gerritsen, G. (author), van der Hoogt, C.C. (author), Schaap, F.G. (author), Voshol, P.J. (author), Kypreos, K.E. (author), Maeda, N. (author), Groen, A.K. (author), Havekes, L.M. (author), Rensen, P.C.N. (author), van Dijk, K.W. (author)
Apolipoprotein E2 (apoE2)-associated hyperlipidemia is characterized by a disturbed clearance of apoE2-enriched VLDL remnants. Because excess apoE2 inhibits LPL-mediated triglyceride (TG) hydrolysis in vitro, we in vestigated whether direct or indirect stimulation of LPL activity in vivo reduces the apoE2-associated hypertriglyc-eridemia. Here,...
article 2008
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TNO Kwaliteit van Leven (author), Kralj, S. (author), van Leeuwen, S.S. (author), Valk, V. (author), Eeuwema, W. (author), Kamerling, J.P. (author), Dijkhuizen, L. (author)
The reuteransucrase enzymes of Lactobacillus reuteri strain 121 (GTFA) and L. reuteri strain ATCC 55730 (GTFO) convert sucrose into alpha-d-glucans (labelled reuterans) with mainly alpha-(1→4) glucosidic linkages (50% and 70%, respectively), plus alpha-(1→6) linkages. In the present study, we report a detailed analysis of various hybrid GTFA/O...
article 2008
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Drissen, R.E.T. (author), Maas, R.H.W. (author), van der Maarel, M.J.E.C. (author), Kabel, M.A. (author), Schols, H.A. (author), Tramper, J. (author), Beeftink, H.H. (author), TNO Kwaliteit van Leven (author)
The kinetics of cellulose hydrolysis by commercially available Cellubrix were described mathematically, with Avicel and wheat straw as substrates. It was demonstrated that hydrolysis could be described by three reactions: direct glucose formation and indirect glucose formation via cellobiose. Hydrolysis did not involve any soluble oligomers...
article 2007
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Kaper, T. (author), Leemhuis, H. (author), Uitdehaag, J.C.M. (author), van der Veen, B.A. (author), Dijkstra, B.W. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)
Glycoside hydrolase family 77 (GH77) belongs to the α-amylase superfamily (Clan H) together with GH13 and GH70. GH77 enzymes are amylomaltases or 4-α-glucanotransferases, involved in maltose metabolism in microorganisms and in starch biosynthesis in plants. Here we characterized the amylomaltase from the hyperthermophilic bacterium Thermus...
article 2007
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Adams, T.B. (author), McGowen, M.M. (author), Williams, M.C. (author), Cohen, S.M. (author), Feron, V.J. (author), Goodman, J.I. (author), Marnett, L.J. (author), Munro, I.C. (author), Portoghese, P.S. (author), Smith, R.L. (author), Waddell, W.J. (author), TNO Kwaliteit van Leven (author)
This publication is the 11th in a series of safety evaluations performed by the Expert Panel of the Flavor and Extract Manufacturers Association (FEMA). In 1993, the Panel initiated a comprehensive program to re-evaluate the safety of more than 1700 GRAS flavoring substances under conditions of intended use. The list of GRAS substances has now...
article 2007
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TNO Kwaliteit van Leven (author), Barends, T.R.M. (author), Bultema, J.B. (author), Kaper, T. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), Dijkstra, B.W. (author)
Amylomaltases are glycosyl hydrolases belonging to glycoside hydrolase family 77 that are capable of the synthesis of large cyclic glucans and the disproportionation of oligosaccharides. Using protein crystallography, we have generated a flip book movie of the amylomaltase catalytic cycle in atomic detail. The structures include a covalent...
article 2007
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TNO Kwaliteit van Leven (author), Moen, C.J.A. (author), Tholens, A.P. (author), Voshol, P.J. (author), de Haan, W. (author), Havekes, L.M. (author), Gargalovic, P. (author), Lusis, A.J. (author), van Dyk, K.W. (author), Frants, R.R. (author), Hofker, M.H. (author), Rensen, P.C.N. (author)
The Hyplip2 congenic mouse strain contains part of chromosome 15 from MRL/MpJ on the BALB/cJ background. Hyplip2 mice show increased plasma levels of cholesterol and predominantly triglycerides (TGs) and are susceptible to diet-induced atherosclerosis. This study aimed at elucidation of the mechanism(s) explaining the hypertriglyceridemia....
article 2007
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Coulier, L. (author), Kaal, E.R. (author), Hankemeier, Th. (author), TNO Kwaliteit van Leven (author)
The hydrolytic degradation of poly(bisphenol A)carbonate (PC) has been characterized by various liquid chromatography techniques. Size exclusion chromatography (SEC) showed a significant decrease in molecular mass as a result of hydrolytic degradation, while 'liquid chromatography at critical conditions' (LC-CC) was very successful for observing...
article 2006
Searched for: subject:"Hydrolysis"
(1 - 20 of 77)

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