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van der Waa, J. (author), Verdult, S. (author), van den Bosch, K. (author), van Diggelen, J. (author), Haije, T. (author), van der Stigchel, B. (author), Cocu, I. (author)With the progress of Artificial Intelligence, intelligent agents are increasingly being deployed in tasks for which ethical guidelines and moral values apply. As artificial agents do not have a legal position, humans should be held accountable if actions do not comply, implying humans need to exercise control. This is often labeled as Meaningful...article 2021
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Krul, L. (author), Kremer, B.H.A. (author), Lucas Luijckx, N.B. (author), Leeman, W.R. (author)The EU Food Supplements Directive (2002/46/EC) mandates the determination of both maximum and minimum permitted levels (MPLs) for micronutrients. In order to determine MPLs which are feasible for particular population groups, a scientific approach should be used in which risk of high intake, risk of inadequacy and benefits are assessed in an...article 2017
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Quantitative protein abundance measurements: absolute quantification of plasma membrane measurementsErpelinck, S. (author), Meijers, T. (author), Ariga, K. (author)Membrane embedded transporter proteins play an important role in the absorption, distribution and excretion of many drugs. A reliable prediction of human pharmacokinetics of (new) drugs based on PBPK modeling is highly dependent on accurate determination of the absolute protein expression levels of these transmembrane transporter proteins.other 2017
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Reus, A.A. (author), Maas, W.J.M. (author), Jansen, H.T. (author), Constant, S. (author), Staal, Y.C.M. (author), van Triel, J.J. (author), Kuper, C.F. (author)The respiratory route is an important portal for human exposure to a large variety of substances. Consequently, there is an urgent need for realistic in vitro strategies for evaluation of the absorption of airborne substances with regard to safety and efficacy assessment. The present study investigated feasibility of a 3D human airway epithelial...article 2014
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Kostewicz, E.S. (author), Abrahamsson, B. (author), Brewster, M. (author), Brouwers, J. (author), Butler, J. (author), Carlert, S. (author), Dickinson, P.A. (author), Dressman, J. (author), Holm, R. (author), Klein, S. (author), Mann, J. (author), McAllister, M. (author), Minekus, M. (author), Muenster, U. (author), Müllertz, A. (author), Verwei, M. (author), Vertzoni, M. (author), Weitschies, W. (author), Augustijns, P. (author)Accurate prediction of the in vivo biopharmaceutical performance of oral drug formulations is critical to efficient drug development. Traditionally, in vitro evaluation of oral drug formulations has focused on disintegration and dissolution testing for quality control (QC) purposes. The connection with in vivo biopharmaceutical performance has...article 2014
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Minekus, M. (author), Alminger, M. (author), Alvito, P. (author), Ballance, S. (author), Bohn, T. (author), Bourlieu, C. (author), Carrière, F. (author), Boutrou, R. (author), Corredig, M. (author), Dupont, D. (author), Dufour, C. (author), Egger, L. (author), Golding, M. (author), Karakaya, S. (author), Kirkhus, B. (author), Le Feunteun, S. (author), Lesmes, U. (author), MacIerzanka, A. (author), MacKie, A. (author), Marze, S. (author), McClements, D.J. (author), Ménard, O. (author), Recio, I. (author), Santos, C.N. (author), Singh, R.P. (author), Vegarud, G.E. (author), Wickham, M.S.J. (author), Weitschies, W. (author), Brodkorb, A. (author)Simulated gastro-intestinal digestion is widely employed in many fields of food and nutritional sciences, as conducting human trials are often costly, resource intensive, and ethically disputable. As a consequence, in vitro alternatives that determine endpoints such as the bioaccessibility of nutrients and non-nutrients or the digestibility of...article 2014
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van Loo-Bouwman, C.A. (author), Naber, T.H.J. (author), Minekus, M. (author), van Breemen, R.B. (author), Hulshof, P.J.M. (author), Schaafsma, G. (author)Since the food matrix determines β-carotene availability for intestinal absorption, food matrix effects on the bioaccessibility of β-carotene from two diets were investigated in vitro and compared with in vivo data. The "mixed diet" consisted of β-carotene-rich vegetables, and the "oil diet" contained β-carotene-low vegetables with supplemental...article 2014
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Martinez, R.C.R. (author), Cardarelli, H.R. (author), Borst, W. (author), Albrecht, S. (author), Schols, H. (author), Gutiérrez, O.P. (author), Maathuis, A.J.H. (author), de Melo Franco, B.D.G. (author), de Martinis, E.C.P. (author), Zoetendal, E.G. (author), Venema, K. (author), Saad, S.M.I. (author), Smidt, H. (author)A validated in vitro model of the large intestine (TIM-2), set up with human or pig faeces, was used to evaluate the impact of potentially probiotic Lactobacillus amylovorus DSM 16698, administered alone (i), in the presence of prebiotic galactooligosaccharides (GOS) (ii), and co-administered with probiotic Bifidobacterium animalis ssp. lactis...article 2013
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Hendriks, H.F.J. (author)In this paper, the nutrigenomics approach is discussed as a research tool to study the physiological effects of nutrition and consequently how nutrition affects health and disease (endpoints). Nutrigenomics is the study of the effects of foods and food constituents on gene expression; the analyses include analysis of mRNA, proteins and...article 2013
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Helbig, A. (author), Silletti, E. (author), van Aken, G.A. (author), Oosterveld, A. (author), Minekus, M. (author), Hamer, R.J. (author), Gruppen, H. (author)This study investigated the effect of gastric passage of protein stabilized emulsions, i.e., whey protein isolate (WPI) and lysozyme, under dynamic in vitro conditions on both the gastric and intestinal lipolysis. Emulsions were prepared at neutral pH to enable an opposite surface charge. Experiments were performed in a multi-compartmental...article 2013
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Balvers, M.G.J. (author), Wortelboer, H.M. (author), Witkamp, R.F. (author), Verhoeckx, K.C.M. (author)The origin of N-acyl ethanolamides (NAEs) in plasma is not well understood, and it is possible that NAEs are present in plasma in esterified form. To test this hypothesis, a new and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of arachidonoyl ethanolamide, 2...article 2013
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Roeselers, G. (author), Ponomarenko, M. (author), Lukovac, S. (author), Wortelboer, H.M. (author)It is increasingly apparent that the microbial ecosystems in the mammalian gastrointestinal tract play an intricate role in health and disease. There is a growing interest in the development of targeted strategies for modulating health through the modification of these microbiota. Ecologists are faced with the challenge of understanding the...article 2013
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van de Steeg, E. (author), Greupink, R. (author), Schreurs, M. (author), Nooijen, I.H.G. (author), Verhoeck, K.C.M. (author), Hanemaaijer, R. (author), Ripken, D. (author), Monshouwer, M. (author), Vlaming, M.L.H. (author), DeGroot, J. (author), Verwei, M. (author), Russel, F.G.M. (author), Huisman, M.T. (author), Wortelboer, H.M. (author)Organic anion-transporting polypeptide 1B1 (OATP1B1) is an important hepatic uptake transporter, of which the polymorphic variant OATP1B1*15 (Asn130Asp and Val174Ala) has been associated with decreased transport activity. Rosuvastatin is an OATP1B1 substrate and often concomitantly prescribed with oral antidiabetics in the clinic. The aim of...article 2013
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Schrieks, I.C. (author), van Berg, R. (author), Sierksma, A. (author), Beulens, J.W.J. (author), Vaes, W.H.J. (author), Hendriks, N.F.J. (author)Aims: To evaluate the effect of acute and chronic consumption of red wine or de-alcoholized red wine with a similar antioxidant capacity on plasma total antioxidant capacity (TEAC), nuclear factor-κB (NF-κB) activity and F2-isoprostanes (8-iso-PGF2α) in healthy men. Methods: Nineteen healthy men with an increased waist circumference (≥94 cm) and...article 2013
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Epitope-distal effects accompany the binding of two distinct antibodies to hepatitis B virus capsidsBereszczak, J.Z. (author), Rose, R.J. (author), van Duijn, E. (author), Watts, N.R. (author), Wingfield, P.T. (author), Steven, A.C. (author), Heck, A.J.R. (author)Infection of humans by hepatitis B virus (HBV) induces the copious production of antibodies directed against the capsid protein (Cp). A large variety of anticapsid antibodies have been identified that differ in their epitopes. These data, and the status of the capsid as a major clinical antigen, motivate studies to achieve a more detailed...article 2013
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Tielemans, S.M.A.J. (author), Geleijnse, J.M. (author), van Baak, M.A. (author), Engberink, M.F. (author), Brink, E.J. (author), de Jong, P.E. (author), Gansevoort, R.T. (author), Bakker, S.J.L. (author)OBJECTIVES: It is not yet clear whether dietary protein could help maintaining a healthy blood pressure (BP). We investigated the association between total protein intake, estimated from 24-h urinary urea excretion, and incident hypertension in Dutch men and women. METHODS: We analyzed data of 3997 men and women (aged 28-75 years) who...article 2013
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Krug, A.K. (author), Kolde, R. (author), Gaspar, J.A. (author), Rempel, E. (author), Balmer, N.V. (author), Meganathan, K. (author), Vojnits, K. (author), Baquié, M. (author), Waldmann, T. (author), Ensenat-Waser, R. (author), Jagtap, S. (author), Evans, R.M. (author), Julien, S. (author), Peterson, H. (author), Zagoura, D. (author), Kadereit, S. (author), Gerhard, D. (author), Sotiriadou, I. (author), Heke, M. (author), Natarajan, K. (author), Henry, M. (author), Winkler, J. (author), Marchan, R. (author), Stoppini, L. (author), Bosgra, S. (author), Westerhout, J. (author), Verwei, M. (author), Vilo, J. (author), Kortenkamp, A. (author), Hescheler, J. (author), Hothorn, L. (author), Bremer, S. (author), van Thriel, C. (author), Krause, K.-H. (author), Hengstler, J.G. (author), Rahnenführer, J. (author), Leist, M. (author), Sachinidis, A. (author)Developmental neurotoxicity (DNT) and many forms of reproductive toxicity (RT) often manifest themselves in functional deficits that are not necessarily based on cell death, but rather on minor changes relating to cell differentiation or communication. The fields of DNT/RT would greatly benefit from in vitro tests that allow the identification...article 2013
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Bentz, J. (author), O'Connor, M.P. (author), Bednarczyk, D. (author), Coleman, J. (author), Lee, C. (author), Palm, J. (author), Pak, Y.A. (author), Perloff, E.S. (author), Reyner, E. (author), Balimane, P. (author), Brännström, M. (author), Chu, X. (author), Funk, C. (author), Guo, A. (author), Hanna, I. (author), Herédi-Szabó, K. (author), Hillgren, K. (author), Li, L. (author), Hollnack-Pusch, E. (author), Jamei, M. (author), Lin, X. (author), Mason, A.K. (author), Neuhoff, S. (author), Patel, A. (author), Podila, L. (author), Plise, E. (author), Rajaraman, G. (author), Salphati, L. (author), Sands, E. (author), Taub, M.E. (author), Taur, J.-S. (author), Weitz, D. (author), Wortelboer, H.M. (author), Xia, C.Q. (author), Xiao, G. (author), Yabut, J. (author), Yamagata, T. (author), Zhang, L. (author), Ellens, H. (author)A P-glycoprotein (P-gp) IC50 working group was established with 23 participating pharmaceutical and contract research laboratories and one academic institution to assess interlaboratory variability in P-gp IC 50 determinations. Each laboratory followed its in-house protocol to determine in vitro IC50 values for 16 inhibitors using four different...article 2013
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Havenaar, R. (author), de Jong, A. (author), Koenen, M.E. (author), van Bilsen, J. (author), Janssen, A.M. (author), Labij, E. (author), Westerbeek, H.J.M. (author)Aim of this study was to investigate the digestion of transglutaminase cross-linked caseinate (XLC) versus native caseinate (NC) in solution and in cheese spread under digestive conditions for adults and children mimicked in a gastrointestinal model. Samples were collected for gel electrophoresis and nitrogen analysis. The results showed no...article 2013
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- de Jong, L. (author) article 2013