- document
-
Hoekstra, S. (author), van Bergen, S. (author), van Brouwershaven, I.R. (author), Schilperoort, R.A. (author), Heidekamp, F. (author), TNO Voeding (author)The influence of 2,4-dichlorophenoxyacetic acid (2,4-D) on embryo-like structures (ELS) and plant development from barley microspores was determined. Microspores cultured on filters enabled simple modification of growth regulator concentrations. Regeneration frequencies obtained with 2,4- D as growth regulator were similar to the results...article 1996
- document
-
van Mechelen, J.R. (author), Smits, M. (author), Douma, A.C. (author), Rouster, J. (author), Cameron-Mills, V. (author), Heidekamp, F. (author), Valk, B.E. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A full length cDNA sequence for a barley grain lipoxygenase was obtained. It includes a 5' untranslated region of 69 nucleotides, an open reading frame of 2586 nucleotides encoding a protein of 862 amino acid residues and a 3' untranslated region of 142 nucleotides. The molecular mass of the encoded polypeptide was calculated to be 96.392. Its...article 1995
- document
-
Instituut voor biotechnologie en chemie TNO Centrum voor Fytotechnologie (author), Hoekstra, S. (author), van Zijderveld, M.H. (author), Louwerse, J.D. (author), Heidekamp, F. (author), van der Mark, F. (author)The influence of environmental and cultural changes was investigated on both anther culture and microspore culture of barley cv. Igri. The highest regeneration frequency for both culture systems was obtained when at least 50% of the microspore population was in the mid-late to late uninucleate stage, when the anthers had been pretreated for 4...article 1992