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Short communication Growth of dairy isolates of Geobacillus thermoglucosidans in skim milk depends on lactose degradation products supplied by Anoxybacillus flavithermus as secondary species

Zhao, Y. (author), Kumar, M. (author), Caspers, M.P.M. (author), Nierop Groot, M.N. (author), van der Vossen, J.M.B.M. (author), Abee, T. (author)

Thermophilic bacilli such as Anoxybacillus and Geobacillus are important contaminants in dairy powder products. Remarkably, one of the common contaminants, Geobacillus thermoglucosidans, showed poor growth in skim milk, whereas significant growth of G. thermoglucosidans was observed in the presence of an Anoxybacillus flavithermus dairy isolate....

article 2018

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Dynamics of red fluorescent dental plaque during experimental gingivitis - A cohort study

van der Veen, M.H. (author), Volgenant, C.M.C. (author), Keijser, B. (author), ten Cate, J.M. (author), Crielaard, W. (author)

Objectives The dynamics of red fluorescent plaque (RFP) in comparison to clinical plaque and bleeding scores were studied during an experimental gingivitis protocol in a cohort of healthy participants. Methods Forty-one participants were monitored for RFP before (24 h plaque), during 14 days plaque accumulation (days 2, 5, 9, 14) and after 7...

article 2016

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Establishment of oxidative D-xylose metabolism in Pseudomonas putida S12

Meijnen, J.P. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author)

The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on D-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g D-xylose-1) and a maximum growth...

article 2009

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Linking phylogenetic identities of bacteria to starch fermentation in an in vitro model of the large intestine by RNA-based stable isotope probing

Kovatcheva-Datchary, P. (author), Egert, M. (author), Maathuis, A. (author), Rajilić-Stojanović, M. (author), de Graaf, A.A. (author), Smidt, H. (author), de Vos, W.M. (author), Venema, K. (author), TNO Kwaliteit van Leven (author)

Summary Carbohydrates, including starches, are an important energy source for humans, and are known for their interactions with the microbiota in the digestive tract. Largely, those interactions are thought to promote human health. Using 16S ribosomal RNA (rRNA)-based stable isotope probing (SIP), we identified starch-fermenting bacteria under...

article 2009

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Transcriptional activity around bacterial cell death reveals molecular biomarkers for cell viability

TNO Kwaliteit van Leven (author), Kort, R. (author), Keijser, B.J. (author), Caspers, M.P.M. (author), Schuren, F.H. (author), Montijn, R. (author)

Background: In bacteriology, the ability to grow in selective media and to form colonies on nutrient agar plates is routinely used as a retrospective criterion for the detection of living bacteria. However, the utilization of indicators for bacterial viability-such as the presence of specific transcripts or membrane integrity-would overcome bias...

article 2008

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Referral patterns of children with poor growth in primary health care

TNO Kwaliteit van Leven (author), Grote, F.K. (author), Oostdijk, W. (author), de Muinck Keizer-Schrama, S.M.P.F. (author), Dekker, F.W. (author), van Dommelen, P. (author), van Buuren, S. (author), Lodder-van der Kooij, A.M. (author), Verkerk, P.H. (author), Wit, J.M. (author)

Background. To promote early diagnosis and treatment of short stature, consensus meetings were held in the mid nineteen nineties in the Netherlands and the UK. This resulted in guidelines for referral. In this study we evaluate the referral pattern of short stature in primary health care using these guidelines, comparing it with cut-off values...

article 2007

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Hypercholesterolemia reduces collateral artery growth more dominantly than hyperglycemia or insulin resistance in mice

van Weel, V. (author), de Vries, M. (author), Voshol, P.J. (author), Verloop, R.E. (author), Eilers, P.H.C. (author), van Hinsbergh, V.W.M. (author), van Bockel, J.H. (author), Quax, P.H.A. (author), TNO Kwaliteit van Leven (author)

OBJECTIVE - Collateral artery development (arteriogenesis), a vital compensatory mechanism in patients with arterial obstructive disease, may be deregulated by vascular risk factors, eg, diabetes or hypercholesterolemia. Here, we compared the effects of either disturbed glucose metabolism or disturbed lipid metabolism on arteriogenesis. METHODS...

article 2006

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Survival, elongation, and elevated tolerance of Salmonella enterica serovar enteritidis at reduced water activity

TNO Defensie en Veiligheid (author), Kieboom, J. (author), Kusumaningrum, H.D. (author), Tempelaars, M.H. (author), Hazeleger, W.C. (author), Abee, T. (author), Beumer, R.R. (author)

Growing microorganisms on dry surfaces, which results in exposure to low water activity (aw), may change their normal morphology and physiological activity. In this study, the morphological changes and cell viability of Salmonella enterica serovar Enteritidis challenged to low a w were analyzed. The results indicated that exposure to reduced aw...

article 2006

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Associations between prenatal and infancy weight gain and BMI, fat mass, and fat distribution in young adulthood: A prospective cohort study in males and females born very preterm

Euser, A.M. (author), Finken, M.J.J. (author), Keijzer-Veen, M.G. (author), Hille, E.T.M. (author), Wit, J.M. (author), Dekker, F.W. (author)

Background: Increasing evidence indicates that adult body composition is associated with prenatal and infancy weight gain, but the relative importance of different time periods has not been elucidated. Objective: The objective was to study the association between prenatal, early postnatal, and late infancy weight gain and body mass index (BMI),...

article 2005

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Time-lapse tracking of barley androgenesis reveals position-determined cell death within pro-embryos

Maraschin, S.D.F. (author), Vennik, M. (author), Lamers, G.E.M. (author), Spaink, H.P. (author), Wang, M. (author)

Following abiotic stress to induce barley (Hordeum vulgare L.) androgenesis, the development of 794 enlarged microspores in culture was monitored by time-lapse tracking. In total, 11% of the microspores tracked developed into embryo-like structures (type-I pathway), 36% formed multicellular structures (type-II pathway) and 53% of the microspores...

article 2005

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14-3-3 Isoforms and pattern formation during barley microspore embryogenesis

de Maraschin, S. (author), Lamers, G.E.M. (author), de Pater, B.S. (author), Spaink, H.P. (author), Wang, M. (author)

The members of the 14-3-3 isoform family have been shown to be developmentally regulated during animal embryogenesis, where they take part in cell differentiation processes. 14-3-3 isoform-specific expression patterns were studied in plant embryogenic processes, using barley (Hordeum vulgare L.) microspore embryogenesis as a model system. After...

article 2003

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Relevance of microbial interactions to predictive microbiology

Malakar, P.K. (author), Barker, G.C. (author), Zwietering, M.H. (author), van 't Riet, K. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)

Microbial interaction can be ignored in predictive microbiology under most conditions. We show that interactions are only important at high population densities, using published data on inhibition of growth of Listeria monocytogenes in broth. Our analysis using growth models from predictive microbiology indicated that interactions only occur at...

article 2003

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Dietary 135-fold cholecalciferol supplementation severely disturbs the endochondral ossification in growing dogs

Tryfonidou, M.A. (author), Holl, M.S. (author), Stevenhagen, J.J. (author), Buurman, C.J. (author), Deluca, H.F. (author), Oosterlaken-Dijksterhuis, M.A. (author), van den Brom, W.E. (author), van Leeuwen, J.P.T.M. (author), Hazewinkel, H.A.W. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)

The effects of excessive non-toxic dietary Vitamin D3 supplementation on Ca homeostasis with specific effects on endochondral ossification and skeletal remodeling were investigated in a group of growing Great Dane dogs supplemented with cholecalciferol (Vitamin D3; HVitD) versus a control group (CVitD) (1350 μg versus 11.4 μg Vitamin D3 per...

article 2003

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A human flora-associated rat model of the breast-fed infant gut

TNO BIBRA (author), Edwards, C.A. (author), Rumney, C. (author), Davies, M. (author), Parrett, A.M. (author), Dore, J. (author), Martin, F. (author), Schmitt, J. (author), Stahl, B. (author), Norin, E. (author), Midtvedt, T. (author), Rowland, I.R. (author), Heavey, P. (author), Köhler, H. (author), Stocks, B. (author), Schroten, H. (author)

Objectives: Bacterial colonization of the infant gut may have important influences on the development of gastrointestinal, respiratory, and allergic disease. Early diet is a major determinant of the gut microflora. It is very difficult to carry out studies in human infants that can investigate the interaction of diet, flora, and mucosa. In this...

article 2003

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The early postnatal development of salivary antibody and immunoglobulin response in children orally colonized with a nonpathogenic, probiotic strain of E. coli

Vančíková, Z. (author), Lodinová-Žádníková, R. (author), Radl, J. (author), Tlaskalová-Hogenová, H. (author)

The development of levels of secretory immunoglobulins (SIgs) in newborns' saliva was examined under physiological conditions and after artificial colonization with nonpathogenic, probiotic bacterial strain E. coli O83. Higher levels of secretory immunoglobulin M (SIgM) and secretory immunoglobulin A (SIgA) were detected in the saliva of breast...

article 2003

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Effects of tylosin on bacterial mucolysis, Clostridium perfringens colonization, and intestinal barrier function in a chick model of necrotic enteritis

TNO Animal Nutrition (author), Collier, C.T. (author), van der Klis, J.D. (author), Deplancke, B. (author), Anderson, D.B. (author), Gaskins, H.R. (author)

Necrotic enteritis (NE) is a worldwide poultry disease caused by the alpha toxin-producing bacterium Clostridium perfringens. Disease risk factors include concurrent coccidial infection and the dietary use of cereal grains high in nonstarch polysaccharides (NSP), such as wheat, barley, rye, and oats. Outbreaks of NE can be prevented or treated...

article 2003

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Haematological characteristics predicting susceptibility for ascites. 1. High carbon dioxide tensions in juvenile chickens

TNO Animal Nutrition (author), Scheele, C.W. (author), van der Klis, J.D. (author), Kwakernaak, C. (author), Buys, N. (author), Decuypere, E. (author)

1. Male broilers of two different genetic stocks, a pure broiler sire line (A) and commercially available Ross broilers (B), were used to study the effect of haematological characteristics in juvenile chickens on the development of clinical ascitic signs. Production performance (body weight gain (BWG), feed intake (FI), feed conversion ratio ...

article 2003

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Haematological characteristics predicting susceptibility for ascites. 2. High haematocrit values in juvenile chickens

TNO Animal Nutrition (author), Scheele, C.W. (author), van der Klis, J.D. (author), Kwakernaak, C. (author), Buys, N. (author), Decuypere, E. (author)

1. Male broilers of two different genetic stocks, a pure broiler sire line (A) and commercially available Ross broilers (B), were used to study the effect of differences in haematocrit values in 12-d-old chickens on the development of ascitic symptoms. Body weight gain (BWG), feed intake (FI) and feed conversion ratio (FRC) were measured from 2...

article 2003

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Biochemical development of subchondral bone from birth until age eleven months and the influence of physical activity

Brama, P.A.J. (author), TeKoppele, J.M. (author), Bank, R.A. (author), Barneveld, A. (author), van Weeren, P.R. (author), Gaubius instituut TNO (author)

Subchondral bone provides structural support to the overlying articular cartilage, and plays an important role in osteochondral diseases. There is growing insight that the mechanical features of bone are related to the biochemistry of the collagen network and the mineral content. In the present study, part of the normal developmental process and...

article 2002

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The value of salivary bacterial counts as a supplement to past caries experience as caries predictor in children

TNO Preventie en Gezondheid (author), van Palenstein Helderman, W.H. (author), Mikx, F.H.M. (author), van 't Hof, M.A. (author), Truin, G.J. (author), Kalsbeek, H. (author)

This study aimed to determine whether salivary mutans streptococci and lactobacilli counts could add any value to a combination of caries experience variables that was recently presented for the prediction of caries. Sixty-nine children at the age of 7.5 yr participated in this longitudinal study. Microbiological data were obtained at the ages...

article 2001