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Diffusion of charged and uncharged contrast agents in equine mandibular condylar cartilage is not affected by an increased level of sugar-induced collagen crosslinking

Mirahmadi, F. (author), Koolstra, J.H. (author), Fazaeli, S. (author), Lobbezoo, F. (author), van Lenthe, G.H. (author), Snabel, J. (author), Stoop, R. (author), Everts, V. (author)

Nutrition of articular cartilage relies mainly on diffusion and convection of solutes through the interstitial fluid due to the lack of blood vessels. The diffusion is controlled by two factors: steric hindrance and electrostatic interactions between the solutes and the matrix components. Aging comes with changes in the cartilage structure and...

article 2019

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Quantitative in vivo CT arthrography of the human osteoarthritic knee to estimate cartilage sulphated glycosaminoglycan content: Correlation with ex-vivo reference standards

van Tiel, J. (author), Siebelt, M. (author), Reijman, M. (author), Bos, P.K. (author), Waarsing, J.H. (author), Zuurmond, A.M. (author), Nasserinejad, K. (author), van Osch, G.J.V.M. (author), Verhaar, J.A.N. (author), Krestin, G.P. (author), Weinans, H. (author), Oei, E.H.G. (author)

Objective. Recently, computed tomography arthrography (CTa) was introduced as quantitative imaging biomarker to estimate cartilage sulphated glycosaminoglycan (sGAG) content in human cadaveric knees. Our aim was to assess the correlation between in vivo CTa in human osteoarthritis (OA) knees and ex vivo reference standards for sGAG and collagen...

article 2016

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Monounsaturated and Saturated, but Not n-6 Polyunsaturated Fatty Acids Decrease Cartilage Destruction under Inflammatory Conditions: A Preliminary Study

Bastiaansen-Jenniskens, Y.M. (author), Siawash, M. (author), van de Lest, C.H.A. (author), Verhaar, J.A.N. (author), Kloppenburg, M. (author), Zuurmond, A.M. (author), Stojanovic-Susulic, V. (author), van Osch, G.J.V.M. (author), Clockaerts, S. (author)

Purpose: Osteoarthritis (OA) is associated with obesity in which altered fatty acid levels have been observed. We investigated whether the most common fatty acids in synovial fluid influence cartilage deterioration in OA. Design: Cartilage was obtained from OA patients undergoing total knee arthroplasty. Chondrocytes or cartilage explants were...

article 2013

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Straining mode-dependent collagen remodeling in engineered cardiovascular tissue

Rubbens, M.P. (author), Mol, A. (author), van Marion, M.H. (author), Hanemaaijer, R. (author), Bank, R.A. (author), Baaijens, F.P.T. (author), Bouten, C.V.C. (author), TNO Kwaliteit van Leven (author)

Similar to native cardiovascular tissues, the mechanical properties of engineered cardiovascular constructs depend on the composition and quality of the extracellular matrix, which is a net result of matrix remodeling processes within the tissue. To improve tissue remodeling, and hence tissue mechanical properties, various mechanical...

article 2009

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Inhibition of glycosaminoglycan incorporation influences collagen network formation during cartilage matrix production

Bastiaansen-Jenniskens, Y.M. (author), Koevoet, W. (author), Jansen, K.M.B. (author), Verhaar, J.A.N. (author), de Groot, J. (author), Vanosch, G.J.V.M. (author), TNO Kwaliteit van Leven (author)

To understand cartilage degenerative diseases and improve repair procedures, we investigate the influence of glycosaminoglycans (GAGs) on cartilage matrix biochemistry and functionality. Bovine articular chondrocytes were cultured in alginate beads with(out) para-nitrophenyl-beta-d-xyloside (PNPX) to inhibit GAG incorporation into newly formed...

article 2009

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Reproducible long-term disc degeneration in a large animal model

Hoogendoorn, R.J.W. (author), Helder, M.N. (author), Kroeze, R.J. (author), Bank, R.A. (author), Smit, T.H. (author), Wuisman, P.I.J.M. (author), TNO Kwaliteit van Leven (author)

STUDY DESIGN. Twelve goats were chemically degenerated and the development of the degenerative signs was followed for 26 weeks to evaluate the progression of the induced degeneration. The results were also compared with a previous study to determine the reproducibility. OBJECTIVES. The purpose of this study was determine whether this...

article 2008

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Molecular changes in the degenerated goat intervertebral disc

Hoogendoorn, R. (author), Doulabi, B.Z. (author), Huang, C.L. (author), Wuisman, P.I. (author), Bank, R.A. (author), Helder, M.N. (author), TNO Kwaliteit van Leven (author)

Study Design. Caprine lumbar intervertebral discs (IVD) were collected from previous studies and categorized as normal, mildly, or severely degenerated. The biochemical composition and the RNA profiles present in both the nucleus pulposus (NP) and the anulus fibrosus (AF) were analyzed. Objective. To determine the molecular changes occurring in...

article 2008

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Age-related accumulation of pentosidine in aggrecan and collagen from normal and degenerate human intervertebral discs

Sivan, S.S. (author), Tsitron, E. (author), Wachtel, E. (author), Roughley, P. (author), Sakkee, N. (author), van der Ham, F. (author), de Groot, J. (author), Maroudas, A. (author), TNO Kwaliteit van Leven (author)

During aging and degeneration, many changes occur in the structure and composition of human cartilaginous tissues, which include the accumulation of the AGE (advanced glycation end-product), pentosidine, in long-lived proteins. In the present study, we investigated the accumulation of pentosidine in constituents of the human IVD (intervertebral...

article 2006

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Biochemical analysis of the articular cartilage and subchondral and trabecular bone of the metacarpophalangeal joint of horses with early osteoarthritis

van der Harst, M.R. (author), de Groot, J. (author), Kiers, G.H. (author), Brama, P.A.J. (author), van de Lest, C.H.A. (author), van Weeren, P.R. (author)

Objective - To assess whether site-related changes in biochemical composition are present in the cartilage and subchondral and trabecular bone of the metacarpophalangeal joint of horses with early osteoarthritis. Sample population - Right metacarpophalangeal joints from 59 mature warmblood horses. Procedure - Biochemical data (cross-link, amino...

article 2005

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Functional adaptation through changes in regional biochemical characteristics during maturation of equine superficial digital flexor tendons

Lin, Y.L. (author), Brama, P.A.J. (author), Kiers, G.H. (author), de Groot, J. (author), van Weeren, P.R. (author), TNO Preventie en Gezondheid (author)

Objective - To quantify and compare biochemical characteristics of the extracellular matrix (ECM) of specimens harvested from tensional and compressive regions of the superficial digital flexor tendon (SDFT) of horses in age classes that include neonates to mature horses. Sample population - Tendon specimens were collected on postmortem...

article 2005

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Relationship between synovial fluid levels of glycosaminoglycans, hydroxyproline and general MMP activity and the presence and severity of articular cartilage change on the proximal articular surface of P1

TNO Preventie en Gezondheid (author), van den Boom, R. (author), van der Harst, M.R. (author), Brommer, H. (author), Brama, P.A.J. (author), Barneveld, A. (author), van Weeren, P.R. (author), de Groot, J. (author)

Reasons for performing study: Osteoarthritis (OA) is one of the most prevalent and disabling chronic conditions affecting horses and leads to degeneration of articular cartilage. Diagnosis is based on clinical signs in combination with radiography, which is relatively insensitive and provides only an indication of accumulated damage. Alternative...

article 2005

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An integral biochemical analysis of the main constituents of articular cartilage, subchondral and trabecular bone

van der Harst, M.R. (author), Brama, P.A.J. (author), van de Lest, C.H.A. (author), Kiers, G.H. (author), de Groot, J. (author), van Weeren, P.R. (author), TNO Kwaliteit van Leven (author)

Objective: In articular joints, the forces generated by locomotion are absorbed by the whole of cartilage, subchondral bone and underlying trabecular bone. The objective of this study is to test the hypothesis that regional differences in joint loading are related to clear and interrelated differences in the composition of the extracellular...

article 2004

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Extracellular matrix changes in early osteochondrotic defects in foals: A key role for collagen?

Gaubius Instituut TNO (author), van de Lest, C.H.A. (author), Brama, P.A.J. (author), van El, B. (author), de Groot, J. (author), van Weeren, P.R. (author)

Osteochondrosis (OC) is the most important developmental orthopaedic disease in the horse. Despite some decades of research, much of the pathogenesis of the disorder remains obscure. Increasing knowledge of articular cartilage development in juvenile animals led to the presumption that the role of collagen in OC might be more important than...

article 2004

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Assessment of the effects of age and joint disease on hydroxyproline and glycosaminoplycan concentrations in synovial fluid from the metacarpophalangeal joint of horses

TNO Preventie en Gezondheid (author), van den Boom, R. (author), Brama, P.A.J. (author), Kiers, G.H. (author), de Groot, J. (author), van Weeren, P.R. (author)

Objective - To assess the effects of age and joint disease on hydroxyproline and glycosaminoglycan (GAG) concentrations in synovial fluid from the metacarpophalangeal joint of horses and evaluate the association of those concentrations with severity of osteoarthritis and general matrix metalloproteinase (MMP) activity. Sample Population -...

article 2004

document

Development of biochemical heterogeneity of articular cartilage: Influences of age and exercise

Brama, P.A.J. (author), Tekoppele, J.M. (author), Bank, R.A. (author), Barneveld, A. (author), van Weeren, P.R. (author), Gaubius Instituut TNO (author)

The objective of this study was to document the development of biochemical heterogeneity from birth to maturity in equine articular cartilage, and to test the hypothesis that the amount of exercise during early life may influence this process. Neonatal foals showed no biochemical heterogeneity whatsoever, in contrast to a clear biochemical...

article 2002

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Age-related decrease in susceptibility of human articular cartilage to matrix metalloproteinase-mediated degradation: The role of advanced glycation end products

de Groot, J. (author), Verzijl, N. (author), Wenting-Van Wijk, M.J.G. (author), Bank, R.A. (author), Lafeber, F.P.J.G. (author), Bijlsma, J.W.J. (author), Tekoppele, J.M. (author), Gaubius Instituut (author)

Objective. Progressive destruction of articular cartilage is a hallmark of osteoarthritis (OA) and rheumatoid arthritis (RA). Age-related changes in cartilage may influence tissue destruction and thus progression of the disease. Therefore, the effect of age-related accumulation of advanced glycation end products (AGEs) on cartilage...

article 2001

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Polylysine as a vehicle for extracellular matrix-targeted local drug delivery, providing high accumulation and long-term retention within the vascular wall

Sakharov, D.V. (author), Jie, A.F.H. (author), Bekkers, M.E.A. (author), Emeis, J.J. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)

We present the first steps in the elaboration of an approach of extracellular matrix-targeted local drug delivery (ECM-LDD), designed to provide a high concentration, ubiquitous distribution, and long-term retention of a drug within the vessel wall after local intravascular delivery. The approach is based on the concept of a bifunctional drug...

article 2001

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Human granzyme B mediates cartilage proteoglycan degradation and is expressed at the invasive front of the synovium in rheumatoid arthritis

Ronday, H.K. (author), van der Laan, W.H. (author), Tak, P.P. (author), de Roos, J.A.D.M. (author), Bank, R.A. (author), Tekoppele, J.M. (author), Froelich, C.J. (author), Hack, C.E. (author), Hogendoorn, P.C.W. (author), Breedveld, F.C. (author), Verheijen, J.H. (author), Gaubius Instituut (author)

Objective. To investigate the cartilage-degrading capacity of granzyme B and the presence of granzyme B-positive cells at sites of erosion in the rheumatoid synovium. Methods. Granzyme B was added to [3H]proline/[35S]sulphate-labelled cartilage matrices and to cartilage explants. Proteoglycan degradation was assessed by the release of 35S and...

article 2001

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Topographical mapping of biochemical properties of articular cartilage in the equine fetlock joint

Brama, P.A.J. (author), Tekoppele, J.M. (author), Bank, R.A. (author), Karssenberg, D. (author), Barneveld, A. (author), van Weeren, P.R. (author)

The aim of this study was to evaluate topographical differences in the biochemical composition of the extracellular matrix of articular cartilage of the normal equine fetlock joint. Water content, DNA content, glycosaminoglycan (GAG) content and a number of characteristics of the collagen network (total collagen content, levels of hydroxylysine-...

article 2000

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Functional adaptation of equine articular cartilage: The formation of regional biochemical characteristics up to age one year

Brama, P.A.J. (author), Tekoppele, J.M. (author), Bank, R.A. (author), Barneveld, A. (author), van Weeren, P.R. (author), Gaubius Instituut TNO (author)

Biochemical heterogeneity of cartilage within a joint is well known in mature individuals. It has recently been reported that heterogeneity for proteoglycan content and chondrocyte metabolism in sheep develops postnatally under the influence of loading. No data exist on the collagen network in general or on the specific situation in the horse....

article 2000