An enzymatic microreactor based on chaotic micromixing for enhanced amperometric detection in a continuous glucose monitoring application

The development of continuous glucose monitoring systems is a major trend in diabetes-related research. Small, easy-to-wear systems which are robust enough to function over many days without maintenance are the goal. We present a new sensing system for continuous glucose monitoring based on a microreactor incorporating chaotic mixing channels....

Quantitative iTRAQ secretome analysis of aspergillus niger reveals novel hydrolytic enzymes

The natural lifestyle of Aspergillus niger made them more effective secretors of hydrolytic proteins and becomes critical when this species were exploited as hosts for the commercial secretion of heterologous proteins. The protein secretion profile of A. niger and its mutant at different pH was explored using iTRAQ-based quantitative proteomics...

An explanation for the combined effect of xylanase-glucose oxidase in dough systems

In the bakery industry, glucose oxidase is usually used in combination with xylanase. Although many theories exist on the mechanism of action of each enzyme, the positive effect of combining the two is as yet unexplained. In this paper we studied a possible basis for this synergy by focusing on the involvement of arabinoxylans. Water-extractable...

Effect of oxidative enzymes on bulk rheological properties of wheat flour doughs

The use of enzymes such as peroxidases or glucose oxidase instead of chemical oxidants is a very interesting option for improving breadmaking performance of doughs. In this study the effect of such enzymes on bulk rheological properties of dough was quantified and their influence on the polymer network in dough deduced. Small deformation...

Influence of inflammatory cells and serum on the performance of implantable glucose sensors

The objective of this investigation was to evaluate the influence of polymorphonuclear granulocytes on the performance of uncoated and cellulose acetate/Nafion® coated amperometric glucose sensors in vitro. The response of these sensors was also investigated in serum. Uncoated and coated sensors showed lower sensitivities to glucose, with a...

Poly(3,4-ethylenedioxythiophene)-based glucose biosensors

Amperometric biosensors for the recognition of glucose oxidase (GOx) based on poly(3,4-ethylenedioxythiophene) (PEDOT) were fabricated for the first time. The resulting biosensor has potential applications for long-term glucose measurements.

Glucose detection at bare and sputtered platinum electrodes coated with polypyrrole and glucose oxidase

Recently a glucose sensor has been developed, which is easily prepared, has a good stability and high glucose response, and is interference free for ascorbic acid. The sensor was made by galvanostatic polymerization of 0.3 M pyrrole in a HEPES buffer pH 7.0 with 200 U ml-1 glucose oxidase (GOx) using a current of 1 μA. In this study, bare and...