Searched for: subject%3A%22Genetic%255C%2BEngineering%22
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Meijnen, J.P. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author)
The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on D-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g D-xylose-1) and a maximum growth...
article 2009
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Koopman, F.W. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author), TNO Kwaliteit van Leven (author)
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to efficiently utilize the C1 compounds methanol and formaldehyde as auxiliary substrate. The hps and phi genes of Bacillus brevis, encoding two key steps of the ribulose monophosphate (RuMP) pathway, were introduced to construct a pathway for the metabolism of the toxic...
article 2009
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TNO-Quality of Life, Business Unit Food and Biotechnology Innovations (author), Meijnen, J.P. (author), Winde, J.H.de (author), Ruijssenaars, H.J. (author)
The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on D-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g D-xylose-1) and a maximum growth...
article 2009
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Nijkamp, K. (author), Westerhof, R.G.M. (author), Ballerstedt, H. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven (author)
A Pseudomonas putida S12 strain was constructed that is able to convert glucose to p-coumarate via the central metabolite l-tyrosine. Efficient production was hampered by product degradation, limited cellular L-tyrosine availability, and formation of the by-product cinnamate via L-phenylalanine. The production host was optimized by inactivation...
article 2007
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Verdoes, J.C. (author), Punt, P.J. (author), Burlingame, R. (author), Bartels, J. (author), van Dijk, R. (author), Slump, E. (author), Meens, M. (author), Joosten, R. (author), Emalfarb, M. (author), TNO Kwaliteit van Leven (author)
A self-replicating vector was designed that enables the construction of complex libraries in the fungus Chiysosporium lucknowense. The circular vector is linearized in vivo and results in a transformation frequency up to 13,000 transformants/ug ofplasmid DNA. Upon prolonged cultivation of the transformants in liquid medium, the linearized vector...
article 2007
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Maraschin, S.D.F. (author), Caspers, M. (author), Potokina, E. (author), Wülfert, F. (author), Graner, A. (author), Spaink, H.P. (author), Wang, M. (author), TNO Kwaliteit van Leven (author)
Different aspects of androgenesis induction have been studied in detail, but little is known about the molecular mechanisms associated with this developmental switch. We have employed macroarrays containing 1421 expressed sequence tags covering the early stages of barley zygotic embryogenesis to compare the gene expression profiles of stress...
article 2006
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TNO Kwaliteit van Leven (author), van Wezel, G.P. (author), Krabben, P. (author), Traag, B.A. (author), Keijser, B.J.F. (author), Kerste, R. (author), Vijgenboom, E. (author), Heijnen, J.J. (author), Kraal, B. (author)
Filamentous actinomycetes are commercially widely used as producers of natural products (in particular antibiotics) and of industrial enzymes. However, the mycelial lifestyle of actinomycetes, resulting in highly viscous broths and unfavorable pellet formation, has been a major bottleneck in their commercialization. Here we describe the...
article 2006
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TNO Kwaliteit van Leven (author), Verheijen, J.H. (author), Huisman, L.G.M. (author), van Lent, N. (author), Neumann, U. (author), Paganetti, P. (author), Erik Hack, C. (author), Bouwman, F. (author), Lindeman, J. (author), Bollen, E.L.E.M. (author), Hanemaaijer, R. (author)
Background: Formation of deposits of the insoluble amyloid β-peptide is believed to be causally related with neurodegeneration in Alzheimer disease (AD). The β-peptide originates from a larger amyloid precursor protein (APP) by the action of proteolytic enzymes. The first proteolytic event leading to amyloid formation is the cleavage of APP by...
article 2006
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TNO Kwaliteit van Leven (author), Lemckert, A.A.C. (author), Grimbergen, J. (author), Smits, S. (author), Hartkoorn, E. (author), Holterman, L. (author), Berkhout, B. (author), Barouch, D.H. (author), Vogels, R. (author), Quax, P. (author), Goudsmit, J. (author), Havenga, M.J.E. (author)
Recombinant adenoviral vectors based on type 5 (rAd5) show great promise as a vaccine carrier. However, neutralizing activity against Ad5 is prevalent and high-titred among human populations, and significantly dampens Ad5-based vaccine modalities. The generation of alternative adenoviral vectors with low seroprevalence thus receives much...
article 2006
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Brul, S. (author), Keijser, B.J.F. (author), van der Spek, H. (author), Oomes, S.J.C.M. (author), Montijn, R. (author), TNO Kwaliteit van Leven (author)
Genomes are being sequenced at an ever-increasing pace. Also genomes of many of the microorganisms of concern to food safety are now known. The next step is of course how to exploit this information in an effective way. This article briefly introduces the issues in the food processing industry as far as microbial food spoilage and safety are...
article 2005
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van der Werf, M.J. (author), TNO Kwaliteit van Leven (author)
Increasingly, microbial production processes are being improved by targeted approaches. In directed strain improvement, the selection of the relevant targets is the limiting step in metabolic engineering. Currently, the identification of leads is still a random process relying largely on expert knowledge. Recently, this approach has been...
article 2005
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Maraschin, S.F. (author), de Priester, W. (author), Spaink, H.P. (author), Wang, M. (author)
Embryogenesis in plants is a unique process in the sense that it can be initiated from a wide range of cells other than the zygote. Upon stress, microspores or young pollen grains can be switched from their normal pollen development towards an embryogenic pathway, a process called androgenesis. Androgenesis represents an important tool for...
article 2005
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Nijkamp, K. (author), van Luijk, N. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven TNO Voeding (author)
A Pseudomonas putida S12 strain was constructed that efficiently produced thefine chemical cinnamic acid from glucose or glycerol via the central metabolite phenylalanine. The gene encoding phenylalanine ammonia lyase from the yeast Rhodosporidium toruloides was introduced. Phenylalanine availability was the main bottleneck in cinnamic acid...
article 2005
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Joosten, V. (author), Roelofs, M.S. (author), van den Dries, N. (author), Goosen, T. (author), Verrips, C.T. (author), van den Hondel, C.A.M.J.J. (author), Lokman, B.C. (author), TNO Kwaliteit van Leven (author)
The Arthromyces ramosus peroxidase gene (arp) was genetically fused to either the 5′- or 3′-terminal ends of the gene encoding llama variable heavy chain antibody fragment VHH R9, resulting in the fusion expression cassettes ARP-R9 or R9-ARP. Aspergillus awamori transformants were obtained which produced up to 30 mg l-1 fusion protein in the...
article 2005
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TNO Kwaliteit van Leven (author), Knippels, L.M.J. (author), Penninks, A.H. (author)
The potential allergenicity of newly introduced proteins in genetically engineered foods has become an important safety evaluation issue. However, to evaluate the potential allergenicity and the potency of new proteins in our food, there are still no widely accepted and reliable test systems. The best-known allergy assessment proposal for foods...
article 2005
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van der Meer, J.R. (author), Tropel, D. (author), Jaspers, M. (author), TNO Voeding (author)
Engineering bacteria for measuring chemicals of environmental or toxicological concern (bioreporter bacteria) has grown slowly into a mature research area. Despite many potential advantages, current bioreporters do not perform well enough to comply with environmental detection standards. Basically, the reasons for this are the lack of...
article 2004
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König, A. (author), Cockburn, A. (author), Crevel, R.W.R. (author), Debruyne, E. (author), Grafstroem, R. (author), Hammerling, U. (author), Kimber, I. (author), Knudsen, I. (author), Kuiper, H.A. (author), Peijnenburg, A.A.C.M. (author), Penninks, A.H. (author), Poulsen, M. (author), Schauzu, M. (author), Wal, J.M. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
This paper provides guidance on how to assess the safety of foods derived from genetically modified crops (GM crops); it summarises conclusions and recommendations of Working Group 1 of the ENTRANSFOOD project. The paper provides an approach for adapting the test strategy to the characteristics of the modified crop and the introduced trait, and...
article 2004
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van den Eede, G. (author), Aarts, H. (author), Buhk, H.J. (author), Corthier, G. (author), Flint, H.J. (author), Hammes, W. (author), Jacobsen, B. (author), Midtvedt, T. (author), van der Vossen, J. (author), von Wright, A. (author), Wackernagel, W. (author), Wilcks, A. (author), TNO Voeding (author)
In 2000, the thematic network ENTRANSFOOD was launched to assess four different topics that are all related to the testing or assessment of food containing or produced from genetically modified organisms (GMOs). Each of the topics was linked to a European Commission (EC)-funded large shared cost action (see http://www.entransfood.com). Since the...
article 2004
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TNO Preventie en Gezondheid (author), Gervois, P. (author), Kleemann, R. (author), Pilon, A. (author), Percevault, F. (author), Koenig, W. (author), Staels, B. (author), Kooistra, T. (author)
The peroxisome proliferator-activated receptor alpha (PPARalpha), which is highly expressed in liver, plays key roles in lipid metabolism and inflammation. Interleukin-6 (IL-6) is the principal inducer of acute phase response (APR) gene expression. In the present study, we demonstrate that chronic treatment with the PPARalpha agonist fenofibrate...
article 2004
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Punt, P.J. (author), Drint-Kuijvenhoven, A. (author), Lokman, B.C. (author), Spencer, J.A. (author), Jeenes, D. (author), Archer, D.A. (author), van den Hondel, C.A.M.J.J. (author)
We have characterized growth and protein processing characteristics of Aspergillus niger strains carrying a disrupted allele of the previously cloned and characterized kexB gene [Appl. Environ. Microbiol. 66 (2000) 363] encoding a furin-type endoprotease. Deletion of the single-copy gene confirms it to be non-essential but disruptant strains...
article 2003
Searched for: subject%3A%22Genetic%255C%2BEngineering%22
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