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Medisch Biologisch Laboratorium TNO (author), Verdoes, J.C. (author), Punt, P.J. (author), Schrickx, J.M. (author), van Verseveld, H.W. (author), Stouthamer, A.H. (author), van den Hondel, C.A.M.J.J. (author)A strategy, based on the usage of the amdS selection marker and a cosmid vector containing four copies of the glucoamylase gene (glaA), was developed to obtain glucoamylase (GLA)-overproducing A. niger strains. With this strategy, fungal strains carrying up to 200 copies of the glaA gene could be isolated at a relatively high frequency. In each...article 1993
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Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author), HogenEsch, H. (author), de Geus, B. (author), Tielen, F. (author), Rozing, J. (author)We investigated the expression of Ly-6.A2 on isolated murine epidermal cells by flow cytometry. Ly-6.A2 was expressed on 61% of keratinocytes and 6% of dendritic epidermal T cells of C57BL mice. Phosphatidylinositol-specific phospholipase C removed Ly-6.A2, indicating that the antigen is anchored to the keratinocyte membrane via a glycosyl...article 1993
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TNO Preventie en Gezondheid Centre for Phytotechnology RUL/TNO (author), Hensgens, L.A.M. (author), de Bakker, E.P.H.M. (author), Os van-Ruygrok, E.P. (author), Rueb, S. (author), van de Mark, F. (author), van der Maas, H.M. (author), van der Veen, S. (author), Kooman-Gersmann, M. (author), Hart, L. (author), Schilperoort, R.A. (author)Transcriptional and translational fusions were made between the reading frame coding for β-D-glucuronidase and sequences of either a constitutively expressed rice gene (GOS2) involved in initiation of translation or a light-inducible rice gene (GOS5). The transient expression of the fusions was studied via particle bombardment of seedling...article 1993
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Medisch Biologisch Laboratorium TNO Centraal Instituut voor Voedingsonderzoek TNO (author), Pouwels, P.H. (author), Leer, R.J. (author)This paper reviews the present knowledge of the structure and properties of small (< 5 kb) plasmids present in Lactobacillus spp. The data show that plasmids from Lactobacillus spp., like many plasmids from other Gram-positive bacteria, display a modular organization and replicate by a mechanism of rolling circle replication. Structurally,...article 1993
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Hermens, A.F. (author), Bentvelzen, P.A.J. (author), Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author)Rat R2k rhabdomyosarcoma cells were transfected with the human H-ras oncogene, which resulted in increased resistance to cell kill in vitro by a single dose of 137Cs γ-rays. A subline carrying one oncogene showed an increase in the quasi-threshold dose (D(q)) from 0.88 to 1.48 Gy. Another subline containing six oncogenes not only had an...article 1992
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van Oers, M.M. (author), Malarme, D. (author), Jore, J.P.M. (author), Vlak, J.M. (author), Medisch Biologisch Laboratorium TNO (author)Two major late proteins, polyhedrin and p10, are synthesized in large quantities in baculovirus infected insect cells. This and the fact that both proteins are dispensable for virus replication, form the basis for the use of these viruses as vector for foreign gene expression. To address the question whether the Autographa californica nuclear...article 1992
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Punt, P.J. (author), Kramer, C. (author), Kuyvenhoven, A. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author), Medisch Biologisch Laboratorium TNO (author)Introduction of a previously identified promoter element of the Aspergillus nidulans gpdA gene (encoding glyceraldehyde-3-phosphate dehydrogenase), the so-called gpd box, into the upstream region of the highly regulated A. nidulans amdS gene (encoding acetamidase), significantly increased (up to 30-fold) the expression of the lacZ reporter gene...article 1992
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Mattern, I.E. (author), van Noort, J.M. (author), van den Berg, P. (author), Archer, D.B. (author), Roberts, I.N. (author), van den Hondel, C.A.M.J.J. (author), Medisch Biologisch Laboratorium TNO (author)In the present study, the extracellular protease activity in a strain of the filamentous fungus Aspergillus niger was investigated and mutant strains deficient in the production of extracellular proteases were isolated. The major protease, which is responsible for 80-85% of the total activity, is aspergillopepsin A, a protein of ca. 43 kDa, the...article 1992
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van den Eertwegh, A.J.M. (author), Boersma, W.J.A. (author), Claassen, E. (author), Medisch Biologisch Laboratorium TNO (author)The spleen is an important lymphoid organ, involved in immune responses against all types of antigen that appear in the circulation. Its complex anatomical organization, with distinct compartments containing specialized cell types, provides a microenvironment which allows different cell-cell interactions and determines the direction of...article 1992
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- Laman, J.D. (author), van den Eertwegh, A.J.M. (author), Parmentier, H.K. (author), Schuurman, H.J. (author), Medisch Biologisch Laboratorium TNO (author) article 1992
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Hensgens, L.A.M. (author), Fornerod, M.W.J. (author), Rueb, S. (author), Winkler, A.A. (author), van der Veen, S. (author), Schilperoort, R.A. (author), Centrum voor Plantenfysiologisch Onderzoek (author)Transcriptional and translational fusions between the reading frame of the β-D-glucuronidase gene (gusA) and the 2′ as well as the 1′ promoter of mannopine synthase (mas), a TR locus of Agrobacterium tumefaciens, were made. The expression of these constructs was studied in the transgenic F1 offspring of independent tobacco transformants at the...article 1992
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de Pater, Sylvia B. (author), van der Mark, F. (author), Rueb, S. (author), Katagiri, F. (author), Chua, N.H. (author), Schilperoort, R.A. (author), Hensgens, L.A.M. (author), Centrum voor Plantenfysiologisch Onderzoek (author)A single copy gene has been isolated, termed GOS2, from rice. Sequence comparison revealed highly similar genes in mammals and yeast, indicating that GOS2 encodes an evolutionary conserved protein. GOS2 mRNA was detected in all tissues examined. When the upstream region was translationally fused to the reporter gene gusA it was found to drive...article 1992
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Lokman, B.C. (author), van Santen, P. (author), Verdoes, J.C. (author), Kruse, J. (author), Leer, R.J. (author), Posno, M. (author), Pouwels, P.H. (author), Medisch Biologisch Laboratorium TNO (author)A cluster of three genes involved in D-xylose catabolism (viz. xylose genes) in Lactobacillus pentosus has been cloned in Escherichia coli and characterized by nucleotide sequence analysis. The deduced gene products show considerable sequence similarity to a repressor protein involved in the regulation of expression of xylose genes in Bacillus...article 1991
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Punt, P.J. (author), Zegers, N.D. (author), Busscher, M. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author), Medisch Biologisch Laboratorium TNO (author)The expression in Aspergillus is described of genes, coding for intracellular and extracellular proteins controlled by the promoter region of the constitutively and efficiently expressed glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) of Aspergillus nidulans. Both the homologous gpdA and the heterologous Escherichia coli β-galactosidase ...article 1991
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Gaubius Institute TNO (author), Koopman, J. (author), Haverkate, F. (author), Briet, E. (author), Lord, S.T. (author)A congenitally abnormal fibrinogen (Vlissingen) was isolated from the blood of a young woman suffering from massive pulmonary embolism. Fibrinogen Vlissingen showed an abnormal clotting time with both thrombin and Reptilase(TM). The release of the fibrino-peptides A and B by thrombin was normal, but fibrin polymerization was impaired both in the...article 1991
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van Hinsbergh, V.W.M. (author), van den Berg, E.A. (author), Fiers, W. (author), Dooijewaard, G. (author), Gaubius Instituut TNO (author)Endothelial cells play an important role in the regulation of fibrinolysis by the production of several key regulatory proteins. The cytokines tumor necrosis factor (TNF), lymphotoxin, and interleukin-1 (IL-1), but not interleukin-6, increase the production of plasminogen activator inhibitor-1 (PAI-1) by endothelial cells, whereas they have no...article 1990
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Burger, H. (author), van Leen, R.W. (author), Dorssers, L.C.J. (author), Persoon, N.L.M. (author), Lemson, P.J. (author), Wagemaker, G. (author), Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author)To enable preclinical studies on homologous interleukin-3 (IL-3) in primate species, we isolated the gene encoding Rhesus monkey IL-3 (RhIL-3). The nucleotide sequence of the RhIL-3 gene displayed 92.9% homology with that of the human IL-3 (hIL-3) gene. The isolated RhIL-3 gene encodes a 143-amino acid (aa) precursor polypeptide, nine C-terminal...article 1990
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Instituut voor Experimentele Gerontologie TNO (author), Radl, J. (author), Punt, Y.A. (author), van den Enden-Vieveen, M.H.M. (author), Bentvelzen, P.A.J. (author), Bakkus, M.H.C. (author), van den Akker T., W. (author), Benner, R. (author)Consistent chromosomal translocations involving the c-myc cellular oncogene and one of the three immunoglobulin loci are typical for human Burkitt's lymphoma, induced mouse plasmacytoma (MPC) and spontaneously arising rat immunocytoma (RIC). Another plasma cell malignancy, multiple myeloma (MM), arising spontaneously in the ageing C57BL/KaLwRij...article 1990
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Instituut voor Experimentele Gerontologie TNO (author), Slagboom, P.E. (author), Leeuw, W.J.F.de (author), Vijg, J. (author)We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65% decrease in the steady state transcript level of TAT in the liver of 24- and 36-month old rats as compared to 6-month old...article 1990
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Medisch Biologisch Laboratorium TNO (author), Punt, P.J. (author), Dingemanse, M.A. (author), Kuyvenhoven, A. (author), Soede, R.D.M. (author), Pouvels, P.H. (author), van den Hondel, C.A.M.J.J. (author)Analysis of the promoter region of the highly expressed Aspergillus nidulans gpdA gene is described. The nucleotide (nt) sequence of a 1.3-kb region upstream from the ATG was determined. Comparison with promoter regions of other Aspergillus and Neurospora genes revealed several regions similar sequence. Both random and site-specific mutations...article 1990