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Gouka, R.J. (author), Hessing, J.G.M. (author), Punt, P.J. (author), Stam, H. (author), Musters, W. (author), van den Hondel, C.A.M.J.J. (author), TNO Voeding (author)A new, highly inducible fungal promoter derived from the Aspergillus awamori 1,4-β-endoxylanase A (exlA) gene is described. Induction analysis, carried out with the wild-type strain in shake flasks, showed that exlA expression is regulated at the transcriptional level. Using a β-glucuronidase (uidA) reporter strategy, D-xylose was shown to be an...article 1996
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Offermans, M.T.C. (author), Struyk, L. (author), de Geus, B. (author), Breedveld, F.C. (author), van den Elsen, P.J. (author), Rozing, J. (author), TNO Preventie en Gezondheid (author)In order to define the extent of T cell heterogeneity and clonality, unique DNA sequences in the junctional region in rearranged T cell receptor (TcR) genes can be studied. For this purpose we have adapted a non-denaturing nucleic acid gel electrophoresis procedure to detect TcR junctional diversity. Detection of junctional diversity is based...article 1996
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Gaubius Instituut TNO (author), van Vlijmen, B.J.M. (author), van Dijk, K.W. (author), van 't Hof, H.B. (author), van Gorp, P.J.J. (author), van der Zee, A. (author), van der Boom, H. (author), Breuer, M.L. (author), Hofker, M.H. (author), Havekesf, L.M. (author)Apolipoprotein E*2(Arg-155 → Cys) (APOE*2) transgenic mice were generated and compared to the previously generated apolipoprotein E*3- Leiden (APOE*3-Leiden) transgenic mice to study the variable expression of hyperlipoproteinemia associated with these two APOE variants. In the presence of the endogenous mouse Apoe gene, the expression of the...article 1996
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Visser, C.J.T. (author), de Weger, R.A. (author), van Blokland, W.T.M. (author), Seifert-Bock, I. (author), Kobrin, M.S. (author), Korc, M. (author), Woutersen, R.A. (author)In the present study, the expression of the epidermal growth factor receptor (EGFR) was investigated in putative preneoplastic and neoplastic acinar cell lesions induced in the rat pancreas by azaserine, using Northern blotting, in situ hybridisation (ISH) and immunohistochemistry. EGFR protein levels were decreased in putative preneoplastic...article 1996
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Centraal Instituut voor Voedingsonderzoek TNO TNO Preventie en Gezondheid (author), Pouwels, P.H. (author), Leer, R.J. (author), Boersma, W.J.A. (author)Oral administration of lactobacilli evokes mucosal and systemic immune responses against epitopes associated with these organisms (Gerritse et al., 1990, 1991). The adjuvant function of different Lactobacillus species was investigated under the conditions of intraperitoneal (i.p.) injection or oral administration. After i.p. injection of...article 1996
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Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author), Lagadec, P. (author), Reveneau, S. (author), Lejeune, P. (author), Pinard, D. (author), Borman, T. (author), Bauer, J. (author), Jeannin, J.F. (author)Although PROb colonic tumor cells are immunosuppressive, the immunomodulator OM 163 induced the disappearance of macroscopic peritoneal nodules in 50% of rats bearing a peritoneal carcinomatosis (p.c.) induced by PROb cells. When the p.o. developed, the number of T lymphocytes was low and the expression of interferon (IFN)-γ mRNA in tumor...article 1996
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Twisk, J. (author), Hoekman, M.F.M. (author), Lehmann, E.M. (author), Meijer, P. (author), Mager, W.H. (author), Princen, H.M.G. (author), TNO Preventie en Gezondheid (author)Evidence from in vivo studies indicates that the bile acid pool and bile acid excretion are increased in humans with diabetes mellitus and in experimental diabetic animals, and that both parameters return to normal levels after administration of insulin. To investigate the biochemical background of these changes, the effects of insulin on bile...article 1995
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Hennis, B.C. (author), van Boheemen, P.A. (author), Wakabayashi, S. (author), Koide, T. (author), Hoffmann, J.J.M.L. (author), Kievit, P. (author), Dooijewaard, G. (author), Jansen, J.G. (author), Klutf, C. (author), TNO Preventie en Gezondheid (author)Two forms of histidine-rich glycoprotein (HRG) were detected on SDS-PAGE by silver staining and immunoblotting after isolation of the protein from pooled plasma using immune-affinity chromatography followed by chromatography with heparin-Sepharose. Both forms were single-chain molecules and the apparent molecular weights of form 1 and form 2...article 1995
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de Maat, M.P.M. (author), de Knijff, P. (author), Green, F.R. (author), Thomas, A.E. (author), Jespersen, J. (author), Kluft, C. (author), TNO Preventie en Gezondheid (author)Elevated plasma fibrinogen levels represent an increased risk for cardiovascular diseased, but the mechanism explaining this association is still not clear. Genetic differences may play a role, because it has been shown that individuals who carry the rare alleles of polymorphisms in the genes for the Bβ-chain (Bcl I and G/A 455) and the Aα-chain...article 1995
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Wolterbeek, A.P.M. (author), Roggeband, R. (author), Baan, R.A. (author), Feron, V.J. (author), Rutten, A.A.J.J.L. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)Vitamin A and β-carotene protect against respiratory tract cancer by inhibiting the formation of DNA damage and controlling cellular proliferation and differentiation, Recently, it has been shown that the p53 tumor-suppressor gene plays a crucial role in the etiology of respiratory tract cancer. In the present study, we investigated the...article 1995
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Wanker, E. (author), Leer, R.J. (author), Pouwels, P.H. (author), Schwab, H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Two Lactobacillus-Escherichia coli shuttle vectors, harbouring the levanase gene from Bacillus subtilis under the control of its own promoter (pLPEW1) or behind the E. coli tac promoter (pE-SIEW2), were constructed. Lactobacillus plantarum showed the same growth characteristics on selective plates and in liquid media containing inulin, after...article 1995
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van Ree, J.H. (author), Hofker, M.H. (author), van den Broek, J.A.A. (author), van Deursen, J.M.A. (author), van der Boom, H. (author), Frants, R.R. (author), Wieringa, B. (author), Havekes, L.M. (author), Gaubius Instituut TNO (author)The function of apolipoprotein (apo) C1 in vivo is not well understood. From in vitro studies it has been reported that an excess of apoC1 relative to apoE inhibits receptor-mediated uptake of remnant lipoproteins. In order to gain a better understanding of the role of apoC1 in lipoprotein metabolism in vivo, we have generated apoC1-deficient...article 1995
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Twisk, J. (author), de Wit, E.C.M. (author), Princen, H.M.G. (author), TNO Preventie en Gezondheid (author)In previous work we have demonstrated suppression of cholesterol 7α-hydroxylase by bile acids at the level of mRNA and transcription, resulting in a similar decline in bile acid synthesis in cultured rat hepatocytes. In view of the substantial contribution of the 'alternative' or '27-hydroxylase' route to total bile acid synthesis, as...article 1995
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Leer, R.J. (author), van der Vossen, J.M.B.M. (author), van Giezen, M. (author), van Noort, J.M. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The genes encoding the production of acidocin B, a bacteriocin produced by Lactobacillus acidophilus strain M46 which is active against Listeria monocytogenes, Clostridium sporogenes, Brochothrix thermosphacta, Lactobacillus fermentum and Lactobacillus delbrueckii subsp. bulgaricus, but inactive against most other Lactobacillus species, were...article 1995
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Offermans, M.T.C. (author), Sonneveld, R.D. (author), Bakker, E. (author), Deutz-Terlouw, P.P. (author), de Geus, B. (author), Rozing, J. (author), TNO Preventie en Gezondheid (author)Two nucleic acid gel electrophoresis techniques were tested as a possible tool for analyzing junctional diversity in rearranged T cell receptor (TcR) sequences in order to define the extent of T cell heterogeneity. For this purpose denaturing gradient gel electrophoresis (DGGE) as well as non-denaturing gel electrophoresis (nDGE) techniques have...article 1995
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van de Kar, N.C.A.J. (author), Kooistra, T. (author), Vermeer, M. (author), Lesslauer, W. (author), Monnens, L.A.H. (author), van Hinsbergh, V.W.M. (author), TNO Preventie en Gezondheid (author)Infections with verocytotoxin (VT) producing Escherichia coil have been strongly implicated in the epidemic form of hemolytic uremic syndrome (HUS). Endothelial damage plays a central role in the pathogenesis of HUS. In vitro studies have shown that VT can damage endothelial cells after interaction with its cellular receptor...article 1995
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Twisk, J. (author), Hoekman, M.F.M. (author), Mager, W.H. (author), Moorman, A.F.M. (author), de Boer, P.A.J. (author), Scheja, L. (author), Princen, H.M.G. (author), Gebhardt, R. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)We investigated the lobular localization and molecular level of expression of cholesterol 7α-hydroxylase and sterol 27-hydroxylase, two key enzymes in bile acid synthesis, in isolated periportal and pericentral hepatocytes and by in situ hybridization of rat liver. Enzyme activity, mRNA, and gene transcription of cholesterol 7α-hydroxylase were...article 1995
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Arts, J. (author), Lansink, T. (author), Grimbergen, J. (author), Toet, K.H. (author), Kooistra, T. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)We have previously shown that the pleiotropic agent sodium butyrate strongly stimulates tissue-type plasminogen activator (t-PA) expression in human umbilical vein endothelial cells (HUVEC). Here we provide the following evidence that the butyrate-induced t-PA expression in HUVEC involves histone H4 acetylation. (1) t-PA induction by butyrate...article 1995
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van Ree, J.H. (author), Gijbels, M.J.J. (author), van den Broek, W.J.A.A. (author), Hofker, M.H. (author), Havekes, L.M. (author), TNO Preventie en Gezondheid (author)Apolipoprotein (apo) E-deficient mice were fed a hypercholesterolemic diet for 14 weeks. Mean serum cholesterol levels rose to 37.5 mM. Upon complete necroscopy, massive xanthomatous lesions were noticed in various tissues, with a predilection for subcutaneous and peritendinous tissues, while control animals on the same diet (3.4 mM serum...article 1995
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- Meulenbelt, I. (author), Droog, S. (author), Trommelen, G.J.M. (author), Boomsma, D.I. (author), Slagboom, P.E. (author), TNO Preventie en Gezondheid (author) article 1995