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Jong, M.C. (author), Dahlmans, V.E.H. (author), van Gorp, P.J.J. (author), Breuer, M.L. (author), Mol, M.J.T.M. (author), van der Zee, A. (author), Frants, R.R. (author), Hofker, M.H. (author), Havekes, L.M. (author), Gaubius Instituut TNO (author)Transgenic mice overexpressing human APOE*3Leiden are highly susceptible to diet-induced hyperlipoproteinemia and atherosclerosis due to a defect in hepatic uptake of remnant lipoproteins. In addition to the human APOE*3Leiden gene, these mice carry the human APOC1 gene (APOE*3Leiden- C1). To investigate the possible effect of simultaneous...article 1996
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Arts, J. (author), Grimbergen, J. (author), Bosma, P.J. (author), Rahmsdorf, H.J. (author), Kooistra, T. (author), Gaubius Laboratory, TNO-PG, Leiden, Netherlands Institut für Genetik, Forschungszentrum Karlsruhe, Germany TNO Preventie en Gezondheid (author)Experiments were designed to clarify the role of c-Jun/c-Fos and of putative phorbol 12-myristate-13-acetate-(PMA)-responsive elements (TREs) in the induction of plasminogen-activator inhibitor 1 (PAI-1) gene transcription in the human hepatoma cell line HepG2 by activators of protein kinase C (PKC). Treatment of HepG2 cells with the phorbol...article 1996
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van Nistelrooy, J.G.M. (author), van den Brink, J.M. (author), van Kan, J.A.L. (author), van Gorcom, R.F.M. (author), de Waard, M.A. (author), Prins Maurits Laboratorium TNO (author)The CYP51 gene encoding eburicol 14α-demethylase (P450(14DM)) was cloned from a genomic library of the filamentous fungal plant pathogen Penicillium italicum, by heterologous hybridisation with the corresponding gene encoding lanosterol 14α-demethylase from the yeast Candida tropicalis. The nucleotide sequence of a 1739-bp genomic fragment and...article 1996
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Laman, J.D. (author), Claassen, E. (author), Noelle, R.J. (author), TNO Preventie en Gezondheid (author)Initially, a role for the interaction between CD40, expressed on B cells, and gp39 (CD40L), expressed on activated T cells, has been defined in humoral immunity. CD40-CD40L interaction is an essential signal for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching. CD40-CD40L interaction is...article 1996
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Visser, C.J.T. (author), Rijksen, G. (author), Woutersen, R.A. (author), de Weger, R.A. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)This study investigates the possibility that the c-Src protein tyrosine kinase is involved in experimental exocrine pancreatic carcinogenesis. Expression and activity of the protooncogene pp60c-src (c-Src) are investigated in acinar pancreatic (pre-) neoplastic lesions induced in rats by azaserine and compared with normal rat pancreas. Low or...article 1996
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van den Akker, Th.W. (author), Radl, J. (author), Franken-Postma, E. (author), Hagemeijer, A. (author), Gaubius Instituut TNO (author)Multiple myeloma (MM) and Waldenström's macroglobulinemia-like lymphoma (MW) appear spontaneously in C57BL/KaLwRij mice at a frequency of 0.5% and 0.2%, respectively. They can readily be propagated by intravenous transfer of mainly bone marrow or spleen cells into syngeneic recipients. Previous studies demonstrated that these mouse malignant...article 1996
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Boot, H.J. (author), Kolen, C.P.A.M. (author), Pouwels, P.H. (author), Prins Maurits Laboratorium TNO (author)The most-dominant surface-exposed protein in many bacterial species is the S-protein. This protein crystallises into a regular monolayer on the outside surface of the bacteria: the S-layer. Lactobacillus acidophilus harbours two S-protein-encoding genes, slpA and slpB, only one of which (slpA) is expressed. In this study, we show by polymerase...article 1996
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Boot, H.J. (author), Pouwels, P.H. (author), Prins Maurits Laboratorium TNO (author)The function of the S-layer, a regularly arranged structure on the outside of numerous bacteria, appears to be different for bacteria living in different environments. Almost no similarity exists between the primary sequences of S-proteins, although their amino acid composition is comparable. S-protein production is directed by single or...article 1996
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Philip, P.A. (author), Souliotis, V.L. (author), Harris, A.L. (author), Salisbury, A. (author), Tates, A.D. (author), Mitchell, K. (author), van Delft, J.H.M. (author), Ganesan, T.S. (author), Kyrtopoulos, S.A. (author), TNO Voeding (author)Dacarbazine (DTIC) is a DNA-methylating drug used in the treatment of malignant melanoma. Among the DNA dducts induced by DTIC are N7-methylguanine (N7-meG) and O6-methylguamne (O6-meG). The latter adduct, in particular, may be important in the mutagenic as well as the cytotoxic activity of DTIC. Repair of O6-meG is carried out by the enzyme O6...article 1996
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Gouka, R.J. (author), Punt, P.J. (author), Hessing, J.G.M. (author), van den Hondel, C.A.M.J.J. (author), TNO Voeding (author)A study was carried out to obtain more insight into the parameters that determine the secretion of heterologous proteins from filamentous fungi. A strategy was chosen in which the mRNA levels and protein levels of a number of heterologous genes of different origins were compared. All genes were under control of the Aspergillus awamori 1,4-β...article 1996
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Henwood, J. (author), van Eggermond, M.C.J.A. (author), Boxel van-Dezaire, A.H.H. (author), Schipper, R. (author), den Hoedt, M. (author), Peijnenburg, A. (author), Sanal, Ö. (author), Ersoy, F. (author), Rijkers, G.T. (author), Zegers, B.J.M. (author), Vossen, J.M. (author), van Tol, M.J.D. (author), van den Elsen, P.J. (author), TNO Preventie en Gezondheid (author)In this study, we have investigated the impact of deficient MHC class II expression on the use of TCRBV6 and TCRBJ gene elements, and on the pattern of amino acid incorporation exhibited in the N1-D-N2 segments of the third complementarity-determining region (CDR3) of these TCRBV6 rearrangements. To this end, we have analyzed circulating T cells...article 1996
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Meulenbelt, I. (author), Williams, G.J. (author), Koppele, J.M.T.E. (author), van Giessen, G.C.D.E. (author), Slagboom, P.E. (author), TNO Preventie en Gezondheid (author), PG (author)We have determined the allele frequencies mad pairwise linkage disequilibria of restriction fragment length polymorphisms (RFLPs) distributed over the entire COL2A1 gene (spanning 23 · 6 kb) in a population of unrelated Dutch Caucasians. Pairwise linkage disequilibrium analysis of RFLP sites between exon 5B and 51 indicated a high degree of...article 1996
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Argnani, A. (author), Leer, R.J. (author), van Luijk, N. (author), Pouwels, P.H. (author), TNO Voeding (author)A protocol was developed for the introduction of foreign plasmid DNA into various Bifidobacterium strains. The method, which is applicable to all Bifidobacterium species tested so far, is based on electroporation of bacteria made competent by preincubation in electroporation buffer for several hours at 4°C. Transformation of Bifidobacterium...article 1996
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Kockx, M. (author), Princen, H.M.G. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)Chemicals/CAS: 5,8,11,14-Eicosatetraynoic Acid, 1191-85-1; Antilipemic Agents; Apolipoprotein A-I; Fibrinogen, 9001-32-5; pirinixic acid, 50892-23-4; Plasminogen Activator Inhibitor 1; Pyrimidines; Receptors, Cytoplasmic and Nuclear; Transcription Factorsarticle 1996
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Kuiper, J. (author), van 't Hof, A. (author), Otter, M. (author), Biessen, E.A.L. (author), Rijken, D.C. (author), van Berkel, T.J.C. (author), Division of Biopharmaceutics, Leiden/Amsterdam Center for Drug Research, Sylvius Laboratory, P. O. Box 9503, 2300 RA Leiden, Netherlands Gaubius Laboratory, TNO Prevention and Health, P.O. Box 430, 2300 AK Leiden, Netherlands (author)The fibrin-specific thrombolyticum tissue-type plasminogen activator (t-PA) has proven to be a potent drug in several clinical trials, but its clinical application is complicated by the rapid clearance of t-PA from the circulation. The rapid plasma clearance of t-PA results from the uptake of t-PA in the liver. t-PA consists of several domains...article 1996
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Bronzova, J. (author), van Duijn, C.M. (author), Havekes, L.M. (author), de Knijff, P. (author), van Broeckhoven, C. (author), Hofman, A. (author), Gaubius Instituut TNO (author)We have studied the relationship between the apolipoprotein E gene (APOE) and the development of myoclonus, tremors, rigidity and seizures in 168 patients with probable early-onset Alzheimer's disease (AD). There was a statistically significantly lower risk of tremor for carriers of the ε4 allele of APOE. This allele was also associated with an...article 1996
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van der Meulen, R.M. (author), Visser, K. (author), Wang, M. (author)We present data to elucidate the involvement of calcium ions in abscisic acid (ABA)-induced gene expression. Modulation of external calcium concentrations was able to affect ABA-induced specific RAB gene expression. At a constant ABA level with increasing extracellular calcium level, an increasing RAB mRNA expression was measured. In barley...article 1996
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Lansink, M. (author), Kooistra, T. (author), Gaubius Laboratory, Leiden, Netherlands Gaubius Laboratory TNO-PG, P.O. Box 2215, 2301 CE Leiden, Netherlands TNO Preventie en Gezondheid (author)We previously showed the involvement of retinoic acid receptor α (RARα) in the induction of tissue-type plasminogen activator (t-PA) synthesis by RA in human umbilical vein endothelial cells (HUVECs). However, the rather slow onset of this induction of t-PA synthesis suggested an indirect role of RARα. Here, we show that the protein synthesis...article 1996
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TNO Nutrition and Food Research (author), van Gemeren, I.A. (author), Beijersbergen, A. (author), Musters, W. (author), Gouka, R.J. (author), van den Hondel, C.A.M.J.J. (author), Verrips, C.T. (author)A synthetic derivative of the cutinase cDNA of Fusarium solani pisi was expressed in Aspergillus awamori using the A. awamori endoxylanase II (exlA) promoter and terminator. The influence of the origin of the pre-sequence and the presence of a pro-sequence on the efficiency of extracellular cutinase production was analyzed in single-copy...article 1996
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Boot, H.J. (author), Kolen, C.P.A.M. (author), Pot, B. (author), Kersters, K. (author), Pouwels, P.H. (author), Prins Maurits Laboratorium TNO (author)Previously we have shown that the type strain of Lactobacillus acidophilus possesses two S-protein-encoding genes, one of which is silent, on a chromosomal segment of 6 kb. The S-protein-encoding gene in the expression site can be exchanged for the silent S-protein-encoding gene by inversion of this slp segment. In this study the presence of S...article 1996