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Arts, J. (author), Herr, I. (author), Lansink, M. (author), Angel, P. (author), Kooistra, T. (author), TNO Preventie en Gezondheid (author)Tissue-type plasminogen activator (t-PA) gene expression in human endothelial cells and HeLa cells is stimulated by the protein kinase C activator phorbol 12-myristate 13-acetate (PMA) at the level of transcription. To study the mechanism of transcriptional regulation, we have characterized a segment of the t-PA gene extending from -135 to +100...article 1997
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- Dell' Acqua, G. (author), Iacoviello, L. (author), D' Orazio, A. (author), Di Bitondo, R. (author), Di Castelnuovo, A. (author), Donati, M.B. (author), Gaubius Instituut TNO (author) article 1997
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Arts, J. (author), Kooistra, T. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)Fibrates are widely used drugs in hyperlipidemic disorders. In addition to lowering serum triglyceride levels, fibrates have also been shown to reduce elevated plasma plasminogen activator inhibitor-1 (PAI-1) levels in vivo. We demonstrate that fibrates suppress PAI-1 synthesis in cultured cynomolgus monkey hepatocytes in a concentration...article 1997
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van der Bom, J.G. (author), de Knijff, P. (author), Haverkate, F. (author), Bots, M.L. (author), Meijer, P. (author), de Jong, P.T.V.M. (author), Hofman, A. (author), Kluft, C. (author), Grobbee, D.E. (author), Gaubius Instituut TNO (author)Background: Impaired fibrinolytic capacity, as assessed by euglobulin clot lysis time or plasma concentration of fibrinolytic parameters, has been associated with an increased risk of myocardial infarction (MI). We studied the association of a polymorphism in the gene for TPA and of plasma concentrations of TPA (antigen and activity) with the...article 1997
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Gaubius Instituut TNO Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Kaptein, A. (author), Neele, D.M. (author), Twisk, J. (author), Hendriks, H.F.J. (author), Kooistra, T. (author), Princen, H.M.G. (author)We showed previously that retinoids stimulate apolipoprotein A-I (apoA- I) synthesis in cultured cynomolgus hepatocytes only after a 24-h lag phase. Here we report on the biochemical background of the slow response, the requirement for high retinoic acid concentrations, and the involvement of different retinoid receptors. The time course of the...article 1997
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Meulenbelt, I. (author), Bijkerk, C. (author), de Wildt, S.C.M. (author), Miedema, H.S. (author), Valkenburg, H.A. (author), Breedveld, F.C. (author), Pols, H.A.P. (author), TeKoppele, J.M. (author), Sloos, V.F.G. (author), Hofman, A. (author), Slagboom, P.E. (author), van Duijn, C.M. (author)Objective. To investigate whether radiographically evident osteoarthritis (ROA) in 55-65-year-old men and women is associated with specific alleles or genotypes of the cartilage matrix protein (CRTM) and cartilage link protein (CRTL1) genes. Methods. Cases were selected from a population-based study on the presence of ROA of the knee or hip....article 1997
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TNO Preventie en Gezondheid (author), van Arkel, C. (author), Hopstaken, C.M. (author), Zurcher, C. (author), Bos, N.A. (author), Kroese, F.G.M. (author), Savelkoul, H.F.J. (author), Benner, R. (author), Radl, J. (author)Monoclonal gammopathies (MG) are monoclonal proliferative disorders of B cells at the differentiation stage of Ig production. They can be detected in the serum, either as transient or as persistent homogenous immunoglobulin (H-Ig) components. The exact phenotype, localization, and cell lineage origin of the precursor cells of MG are unknown, but...article 1997
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Centraal Instituut voor Voedingsonderzoek TNO (author), Bahl, H. (author), Scholz, H. (author), Bayan, N. (author), Chami, M. (author), Leblon, G. (author), Gulik-Krzywicki, T. (author), Shechter, E. (author), Fouet, A. (author), Mesnage, S. (author), Tosi-Couture, E. (author), Gounon, P. (author), Mock, M. (author), de Macario, E.C. (author), Macario, A.J.L. (author), Fernández-Herrero, L.A. (author), Olabarría, G. (author), Berenguer, J. (author), Blaser, M.J. (author), Kuen, B. (author), Lubitz, W. (author), Sára, M. (author), Pouwels, P.H. (author), Kolen, C.P.A.M. (author), Boot, H.J. (author), Palva, A. (author), Truppe, M. (author), Howorka, S. (author), Schroll, G. (author), Lechleitner, S. (author), Resch, S. (author)In this chapter we report on the molecular biology of crystalline surface layers of different bacterial groups. The limited information indicates that there are many variations on a common theme. Sequence variety, antigenic diversity, gene expression, rearrangements, influence of environmental factors and applied aspects are addressed. There is...article 1997
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Kappers, W.A. (author), de Groene, E.M. (author), Kleij, L.A. (author), Witkamp, R.F. (author), Zweers-Zeilmaker, W.M. (author), Feron, V.J. (author), Horbach, G.J. (author), TNO Voeding (author)1. To study the role of cytochrome P4502C10 in the metabolism of the non-steroidal antiinflammatory drugs (NSAIDs) diclofenac, phenylbutazone, fenoprofen, ibuprofen, flurbiprofen, ketoprofen and naproxen, a cell line was developed stably expressing CYP2C10 cDNA. A retroviral vector construct, containing a human CYP2C10 cDNA, was transfected in...article 1996
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Nijhuis, E.W.P. (author), Oostervink, F. (author), Hinloopen, B. (author), Rozing, J. (author), Nagelkerken, L. (author), Gaubius Instituut TNO (author)Peripheral blood mononuclear cells (PBMC) from 40 consecutive patients entering a screening program on cognitive impairment were studied in vitro with respect to their sensitivity to dexamethasone (DEX). Phytohemagglutinin-induced proliferation by PBMC from patients with senile dementia of the Alzheimer type (SDAT) was less sensitive to the...article 1996
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van Noort, J.M. (author), TNO Preventie en Gezondheid (author)The pathogenesis of multiple sclerosis (MS), the major neurological disease of young adults in the Western world, is still poorly understood, and no effective therapy to block MS is available as yet. The clinical symptoms of MS result from inflammatory damage to the insulating myelin sheath of axons in the CNS and - at later stages - to axons...article 1996
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Gouka, R.J. (author), Stam, H. (author), Fellinger, A.J. (author), Muijsenberg, R.J.G.T. (author), van de Wijngaard, A.J. (author), Punt, P.J. (author), Musters, W. (author), van den Hondel, C.A.M.J.J. (author), TNO Voeding (author)In this study, induction and repression kinetics of the expression of the Aspergillus awamori 1,4-β-endoxylanase A (exlA) gene under defined physiological conditions was analyzed at the mRNA and the protein levels. Induction was analyzed by pulsing D-xylose to a sucrose-limited continuous culture of an A. awamori 1,4-β-endoxylanase A (EXLA)...article 1996
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Hoffer, M.J.V. (author), Bredie, S.J.H. (author), Boomsma, D.I. (author), Reymer, P.W.A. (author), Kastelein, J.J.P. (author), de Knijff, P. (author), Demacker, P.N.M. (author), Stalenhoef, A.F.H. (author), Havekes, L.M. (author), Frants, R.R. (author), Gaubius Instituut TNO (author)Familial combined hyperlipidaemia (FCHL) is one of the major genetic causes of coronary heart disease (CHD) and is characterised by elevated levels of plasma cholesterol and/or triglycerides in individuals within a single family. Decreased lipoprotein lipase (LPL) activity has been found in some cases of FCHL. A recent study revealed a common...article 1996
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Cassee, F.R. (author), Groten, J.P. (author), Feron, V.J. (author), TNO Voeding (author)Formaldehyde, acetaldehyde, and acrolein are well-known upper respiratory tract irritants and occur simultaneously as pollutants in many indoor and outdoor environments. The upper respiratory tract, and especially the nose, is the prime target for inhaled aldehydes. To study possible additive or interactive effects on the nasal epithelium we...article 1996
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van den Brink, H.J.M. (author), van Nistelrooy, H.J.G.M. (author), de Waard, M.A. (author), van den Hondel, C.A.M.J.J. (author), van Gorcom, R.F.M. (author), Prins Maurits Laboratorium TNO (author)In this paper we describe the effects of over-expression of the Penicillium italicum gene encoding eburicol 14α-demethylase (cyp51), in Aspergillus niger strains with one or multiple copies of the gene encoding cytochrome P450 reductase (cpr A), on the eburicol 14α-demethylase activity. Eburicol 14α-demethylase activity was determined by...article 1996
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van Leeuwen, R.T.J. (author), Quax, P.H.A. (author), Tippins, J.R. (author), Antoniw, J.W. (author), Andreotti, F. (author), Maseri, A. (author), Kluft, C. (author), Sperti, G. (author), Gaubius Instituut TNO (author)Background: Lipopolysaccharide (endotoxin) has been shown to increase the expression of plasminogen activator inhibitor type-1 (PAI-1) in the vessel wall. Endotoxin is known to increase PAI-1 production in endothelial cells, but its action on smooth muscle cells (SMCs) is presently not clear. In this study we determined the effect of endotoxin...article 1996
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Boot, H.J. (author), Kolen, C.P.A.M. (author), Andreadaki, F.J. (author), Leer, R.J. (author), Pouwels, P.H. (author), Prins Maurits Laboratorium TNO (author)S-proteins are proteins which form a regular structure (S-layer) on the outside of the cell walls of many bacteria. Two S-protein-encoding genes are located in opposite directions on a 6.0-kb segment of the chromosome of Lactobacillus acidophilus ATCC 4356 bacteria. Inversion of this chromosomal segment occurs through recombination between two...article 1996
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Jong, M.C. (author), Dahlmans, V.E.H. (author), van Gorp, P.J.J. (author), Breuer, M.L. (author), Mol, M.J.T.M. (author), van der Zee, A. (author), Frants, R.R. (author), Hofker, M.H. (author), Havekes, L.M. (author), Gaubius Instituut TNO (author)Transgenic mice overexpressing human APOE*3Leiden are highly susceptible to diet-induced hyperlipoproteinemia and atherosclerosis due to a defect in hepatic uptake of remnant lipoproteins. In addition to the human APOE*3Leiden gene, these mice carry the human APOC1 gene (APOE*3Leiden- C1). To investigate the possible effect of simultaneous...article 1996
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Arts, J. (author), Grimbergen, J. (author), Bosma, P.J. (author), Rahmsdorf, H.J. (author), Kooistra, T. (author), Gaubius Laboratory, TNO-PG, Leiden, Netherlands Institut für Genetik, Forschungszentrum Karlsruhe, Germany TNO Preventie en Gezondheid (author)Experiments were designed to clarify the role of c-Jun/c-Fos and of putative phorbol 12-myristate-13-acetate-(PMA)-responsive elements (TREs) in the induction of plasminogen-activator inhibitor 1 (PAI-1) gene transcription in the human hepatoma cell line HepG2 by activators of protein kinase C (PKC). Treatment of HepG2 cells with the phorbol...article 1996
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van Nistelrooy, J.G.M. (author), van den Brink, J.M. (author), van Kan, J.A.L. (author), van Gorcom, R.F.M. (author), de Waard, M.A. (author), Prins Maurits Laboratorium TNO (author)The CYP51 gene encoding eburicol 14α-demethylase (P450(14DM)) was cloned from a genomic library of the filamentous fungal plant pathogen Penicillium italicum, by heterologous hybridisation with the corresponding gene encoding lanosterol 14α-demethylase from the yeast Candida tropicalis. The nucleotide sequence of a 1739-bp genomic fragment and...article 1996