Searched for: subject%3A%22Gelatinases%22
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TNO Kwaliteit van Leven (author), Sluijter, J.P.G. (author), Verloop, R.E. (author), Pulskens, W.P.C. (author), Velema, E. (author), Grimbergen, J.M. (author), Quax, P.H. (author), Goumans, M.J. (author), Pasterkamp, G. (author), Kleijn, D.P.V.de (author)
Background: Furin-like proprotein convertases (PCs) are proteolytic activators of proproteins, like membrane type 1-matrix metalloproteinase (MT1-MMP) and transforming growth factor beta (TGF-beta), that are described in the arterial response to injury. However, the involvement of furin-like PCs in the arterial response to injury has not been...
article 2005
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Lindeman, J.H.N. (author), Hanemaaijer, R. (author), Mulder, A. (author), Dijkstra, P.D.S. (author), Szuhai, K. (author), Bromme, D. (author), Verheijen, J.H. (author), Hogendoorn, P.C.W. (author)
Giant cell tumor (GCT) of bone is a neoplasm of bone characterized by a localized osteolytic lesion. The nature of GCT is an enigma and the cell type(s) and protease(s) responsible for the extensive localized clinicoradiological osteolysis remain unresolved. We evaluated protease expression and cellular distribution of the proteolytic machinery...
article 2004
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Plaisier, M. (author), Kapiteijn, K. (author), Koolwijk, P. (author), Fijten, C. (author), Hanemaaijer, R. (author), Grimbergen, J.M. (author), Mulder-Stapel, A. (author), Quax, P.H.A. (author), Helmerhorst, F.M. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
In the endometrium, angiogenesis is a physiological process, whereas in most adult tissues neovascularization is initiated only during tissue repair or pathological conditions. Pericellular proteolysis plays an important role in angiogenesis being required for endothelial cell migration, invasion, and tube formation. We studied the expression of...
article 2004
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TNO Preventie en Gezondheid (author), Vernooy, J.H.J. (author), Lindeman, J.H.N. (author), Jacobs, J.A. (author), Hanemaaijer, R. (author), Wouters, E.F.M. (author)
Background: The increased expression of matrix metalloproteinases (MMPs) is considered to be a key factor in the development of COPD. Net MMP activity represents a tightly regulated process, which is not addressed by conventional investigation methods such as messenger RNA or protein expression. Yet, quantitative data on MMP activity in the...
article 2004
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Gaubius Instituut TNO (author), Tchetverikov, I. (author), Ronday, H.K. (author), van El, B. (author), Kiers, G.H. (author), Verzijl, N. (author), TeKoppele, J.M. (author), Huizinga, T.W.J. (author), de Groot, J. (author), Hanemaaijer, R. (author)
Objective: To analyse matrix metalloproteinases (MMPs) and tissue inhibitor-1 of MMPs (TIMP-1) levels in the systemic circulation and synovial fluid (SF) of patients with RA and to compare these levels with inflammatory and collagen degradation markers. Methods: ProMMP-1, -2, -3, -8, -9, TIMP-1, levels of MMP/ α2-macroglobulin complexes, and...
article 2004
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TNO Preventie en Gezondheid (author), Jukema, J.W. (author), van der Hoorn, J.W.A. (author)
Hypertension and hyperlipidaemia are major risk factors for the development of atherosclerosis. Calcium channel blockers (CCBs) have been used for decades and have established antihypertensive effects. Statins have been extensively used because of their potent lipid lowering properties. Amongst other factors, inflammation and oxidation are...
article 2004
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Heikkilä, P. (author), Teronen, O. (author), Hirn, M.Y. (author), Sorsa, T. (author), Tervahartiala, T. (author), Salo, T. (author), Konttinen, Y.T. (author), Halttunen, T. (author), Moilanen, M. (author), Hanemaaijer, R. (author), Laitinen, M. (author)
Background. Bisphosphonates reduce the bone metastasis formation and angiogenesis but the exact molecular mechanisms involved are unclear. Progelatinase A (proMMP-2; 78 KDa) is activated up during the tumor spread and metastasis by a cell surface-associated matrix metalloproteinase (membrane-type matrix metalloproteinase [MT1-MMP] or MMP-14)....
article 2003
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TNO Preventie en Gezondheid (author), Tchetverikov, I. (author), Lard, L.R. (author), de Groot, J. (author), Verzijl, N. (author), TeKoppele, J.M. (author), Breedveld, F.C. (author), Huizinga, T.W.J. (author), Hanemaaijer, R. (author)
Objective: To analyse the relation between systemic levels of pro-MMP-3, -8, and -9 matrix metalloproteinase (MMP) activity in α2 macroglobulin (α2M)/MMP complexes and the progression of joint destruction in patients with recent onset rheumatoid arthritis (RA). Methods: 109 patients with RA of recent onset were entered into this longitudinal...
article 2003
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TNO Preventie en Gezondheid (author), Starckx, S. (author), van den Steen, P.E. (author), Verbeek, R. (author), van Noort, J.M. (author), Opdenakker, G. (author)
The small heat shock protein αB-crystallin is considered as a candidate autoantigen in multiple sclerosis (MS) lesions. Gelatinase B or matrix metalloproteinase (MMP)-9 is a proteinase establishing various disease-promoting feedback loops in autoimmune diseases. Human αB-crystallin was digested with natural gelatinase B and all cleavage sites...
article 2003
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TNO Preventie en Gezondheid (author), Tolboom, T.C.A. (author), Pieterman, E. (author), van der Laan, W.H. (author), Toes, R.E.M. (author), Huidekoper, A.L. (author), Nelissen, R.G.H.H. (author), Breedveld, F.C. (author), Huizinga, T.W.J. (author)
Background: Matrix metalloproteinases (MMPs) have a pivotal role in the destruction of cartilage in rheumatoid arthritis (RA), which is mediated by the fibroblast-like synoviocytes (FLS). Objective: To examine the in vitro invasiveness of synoviocytes obtained from inflamed joints of patients with arthritis in relation to the expression of MMP 1...
article 2002
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TNO Preventie en Gezondheid (author), Waas, E.T. (author), Lomme, R.M.L.M. (author), DeGroot, J. (author), Wobbes, T. (author), Hendriks, T. (author)
The bioactivity of matrix metalloproteinases was studied in tissues from colorectal cancer patients by means of both quantitative gelatin zymography and a fluorometric activity assay. Next to paired samples of tumour tissue and distant normal mucosa (n=73), transitional tissue was analysed from a limited (n=33) number of patients. Broad-spectrum...
article 2002
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Mansell, J.P. (author), Kerrigan, J. (author), McGill, J. (author), Bailey, J. (author), TeKoppele, J. (author), Sandy, J.R. (author)
Cleft lip and palate is a common craniofacial malformation in man. The aetiology is multifactorial and not known. Since collagen is a major structural component of the developing palate, we studied its composition and metabolism during palate shelf formation and elevation in the rat. Palatal shelves were harvested at embryonic days (E) 15, 16...
article 2000
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Hanemaaijer, R. (author), Visser, H. (author), Konttinen, Y.T. (author), Koolwijk, P. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)
Here we describe a new principle for accessing the activity of the different members of the human matrix-metalloproteinases (MMPs) by a colorimetric assay. Using protein engineering, a modified pro-urokinase was made in which the activation sequence, normally recognized by plasmin (ProArgPheLys ↓ IlelleGlyGly), was replaced by a sequence that is...
article 1998
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Konttinen, Y.T. (author), Halinen, S. (author), Hanemaaijer, R. (author), Sorsa, T. (author), Hietanen, J. (author), Ceponis, A. (author), Xu, J.W. (author), Manthorpe, R. (author), Whittington, J. (author), Larsson, Å. (author), Salo, T. (author), Kjeldsen, L. (author), Stenman, U.-H. (author), Eisen, A.Z. (author), Gaubius Instituut TNO (author)
Type IV collagenases/gelatinases (matrix metalloproteinases MMP-2 and MMP-9) in labial salivary glands (LSG) and saliva in Sjogren's syndrome (SS) and healthy controls were studied. Zymograms and Western blots disclosed that SS saliva contained 92/82 kD MMP-9/type IV collagenase duplex. Specific activity measurement disclosed 53.1 ± 9.8 U/mg...
article 1998
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TNO Preventie en Gezondheid Gaubius Instituut TNO (author), Sier, C.F.M. (author), Kubben, F.J.G.M. (author), Ganesh, S. (author), Heerding, M.M. (author), Griffioen, G. (author), Hanemaaijer, R. (author), van Krieken, J.H.J.M. (author), Lamers, C.B.H.W. (author), Verspaget, H.W. (author)
Proteinases are involved in tumour invasion and metastasis. Several matrix metalloproteinases (MMPs) have been shown to be increased in various human carcinomas. We assessed the levels of MMP-2 (gelatinase A) and MMP-9 (gelatinase B) in 50 gastric carcinomas and corresponding mucosa using quantitative gelatin zymography. Both MMP levels were...
article 1996
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de Bart, A.C.W. (author), Quax, P.H.A. (author), Löwik, C.W.G.M. (author), Verheijen, J.H. (author), TNO Preventie en Gezondheid (author)
Plasmin-mediated extracellular proteolysis has been implicated in the degradation of bone in normal and pathological conditions. Normal and malignant osteoblasts can produce both tissue-type plasminogen activator (t- PA) and urokinase-type plasminogen activator (u-PA). We have used the osteosarcoma cell line MG63 to address the question of...
article 1995
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