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van Zeijl, C.M.J. (author), van de Kamp, E.H.M. (author), Punt, P.J. (author), Selten, G.C.M. (author), Hauer, B. (author), van Gorcom, R.F.M. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A method was developed to perform PCR directly on mycelial pellets or colonies treated with NOVOzym 234. The method allows rapid screening of large numbers of transformants of both sporulating and non-sporulating fungi for the presence of (co)transforming plasmid copies or for specific genetic modifications such as gene disruption and site...article 1998
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Smits, J.P. (author), Rinzema, A. (author), Tramper, J. (author), van Sonsbeek, H.M. (author), Hage, J.C. (author), Kaynak, A. (author), Knol, W. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)In this paper, correlations between temperature and kinetics of Trichoderma reesei QM9414 growing on wheat bran are described in terms of glucosamine production, oxygen consumption, and carbon dioxide production. Maximum growth temperature (T(max)) being approximately 314 K, is estimated by measuring the radial growth rate on wheat bran. The...article 1998
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- Frenken, L.G.J. (author), Hessing, J.G.M. (author), van den Hondel, C.A.M.J.J. (author), Verrips, C.T. (author), Centraal Instituut voor Voedingsonderzoek TNO (author) article 1998
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de Nijs, M. (author), van Egmond, H.P. (author), Nauta, M. (author), Rombouts, F.M. (author), Notermans, S.H.W. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Fumonisin B1 is currently regarded as the most significant mycotoxin produced by Fusarium spp. It has carcinogenic properties and may play a role in the etiology of human esophageal cancer. The human population is exposed to fumonisin B1 primarily by intake of fumonisin B1-contaminated maize. Maize consumed in the Netherlands is imported from...article 1998
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van den Brink, H.M. (author), van Gorcom, R.F.M. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The involvement of cytochrome P450 enzymes in many complex fungal bioconversion processes has been characterized in recent years. Accordingly, there is now considerable scientific interest in fungal cytochrome P450 enzyme systems. In contrast to S. cerevisiae, where surprisingly few P450 genes have been identified, biochemical data suggest that...article 1998
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Punt, P.J. (author), van Gemeren, I.A. (author), Drint-Kuijvenhoven, J. (author), Hessing, J.G.M. (author), Muijlwijk van - Harteveld, G.M. (author), Beijersbergen, A. (author), Verrips, C.T. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The function of the endoplasmic-reticulum-localized chaperone binding protein (BiP) in relation to protein secretion in filamentous fungi was studied. It was shown that the overproduction of several homologous and heterologous recombinant proteins by Aspergillus strains induces the expression of bipA, the BiP-encoding gene from Aspergillus niger...article 1998
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Gouka, R.J. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)In this study we have analyzed the effects of a glucoamylase gene fusion on the mRNA levels and protein levels for the human interleukin-6 gene (hil6) and the guar α-galactosidase gene (aglA). Previously it was shown that production of nonfused α-galactosidase and hIL-6 in Aspergillus awamori was limited at transcriptional and (post...article 1997
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van den Brink, H.J.M. (author), van Nistelrooy, H.J.G.M. (author), de Waard, M.A. (author), van den Hondel, C.A.M.J.J. (author), van Gorcom, R.F.M. (author), Prins Maurits Laboratorium TNO (author)In this paper we describe the effects of over-expression of the Penicillium italicum gene encoding eburicol 14α-demethylase (cyp51), in Aspergillus niger strains with one or multiple copies of the gene encoding cytochrome P450 reductase (cpr A), on the eburicol 14α-demethylase activity. Eburicol 14α-demethylase activity was determined by...article 1996
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Rosén, S. (author), Kata, M. (author), Persson, Y. (author), Lipniunas, P.H. (author), Wikström, M. (author), van den Hondel, C.A.M.J.J. (author), van den Brink, J.M. (author), Rask, L. (author), Hedén, L.O. (author), Tunlid, A. (author), TNO Voeding (author)It has been proposed that the interactions between several parasite and pathogenic fungi and their hosts are mediated by soluble lectins present in the fungus. We have cloned and analyzed a gene encoding such a lectin (AOL) from the nematophagous fungus Arthrobotrys oligospora (deuteromycete). The deduced primary structure of the AOL gene...article 1996
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- Centraal Instituut voor Voedingsonderzoek TNO (author), Gouka, R.J. (author) doctoral thesis 1996
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Gouka, R.J. (author), Hessing, J.G.M. (author), Stam, H. (author), Musters, W. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A homologous gene transfer system for Aspergillus awamori for site-specific integration is described, based on two components. First, a defined A. awarnori pyrG mutant strain constructed by a selection strategy for gene-replacement in fungi. Second, a vector with a homologous pyrG selection marker containing a defined mutation at a site...article 1995
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van Gemeren, I.A. (author), Musters, W. (author), van den Hondel, C.A.M.J.J. (author), Verrips, C.T. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A copy of the cutinase cDNA from Fusarium solani pisi was constructed starting from synthetic oligonucleotides. For this construction three separate cassettes were made, which were subsequently assembled to form the cutinase gene. Heterologous expression of the synthetic cutinase gene and the subsequent secretion of the recombinant enzyme was...article 1995
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Instituut voor Milieuwetenschappen TNO (author), Cox, H.H.J. (author)Styrene is an environmental pollutant, emitted in large quantities to the atmosphere by various industrial sectors. Legislation requires industry to reduce the emission of styrene. One option to purify industrial waste gases is biological treatment in biofilters.doctoral thesis 1995
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Punt, P.J. (author), Veldhuisen, G. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Although the application of filamentous fungi, such as Aspergillus niger for the production of extracellular proteins is well established for several decades, hardly any information is available about the molecular mechanisms of the process of protein secretion in these organisms. Two lines of research initiated towards a systematic analysis of...article 1994
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Verdoes, J.C. (author), Calil, M.R. (author), Punt, P.J. (author), Debets, A.J.M. (author), Swart, K. (author), Stouthamer, A.H. (author), van den Hondel, C.A.M.J.J. (author)A method is described for unambiguous assignment of cloned genes to Aspergillus niger chromosomes by CHEF gel electrophoresis and Southern ana lysis. All of the eight linkage groups (LGs), with the exception of LG VII, have previously been assigned to specific chromosomal bands in the electrophoretic karyotype of A. niger. Using a LG VII...article 1994
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- TNO Voeding (author), Verdoes, J.C. (author) doctoral thesis 1994
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- Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), van den Hondel, C.A.M.J.J. (author), Goosen, T. (author) article 1994
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- TNO Building and Construction Research (author), Adan, O.C.G. (author), Samson, R.A. (author), Wijnen, J.T.M. (author) bookPart 1994
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- TNO Voeding (author), Punt, P.J. (author) doctoral thesis 1992
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van Noort, J.M. (author), van den Berg, P. (author), Mattern, I.E. (author), Medisch Biologisch Laboratorium TNO (author)A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel...article 1991